Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 99080515

GI number: 99080515

Start: 721127

End: 721825

Strand: Reverse

Name: cobB [H]

Synonym: TM1040_0674

Alternate gene names: 99080515

Gene position: 721825-721127 (Counterclockwise)

Preceding gene: 99080517

Following gene: 99080510

Centisome position: 22.55

GC content: 59.94

Gene sequence:

>699_bases
ATGAAAAAAATCGTGATCCTCACCGGCGCAGGAATTTCAGCAGAAAGCGGGCTTGGCACGTTTCGCGATAAAGGAGGCCT
GTGGTCGCAGCATCGGATCGAGGATGTTGCAACGCCCGAAGGATTTGCTCGCGATCCGGAACTTGTGCACAGGTTTTATT
CCGCACGGCGCCAGCACGCGGCAGAAGCCCTACCCAACGCGGCCCATGACGCGCTGGCACGTCTTGAAGAACGGTGGACT
GGTGACGTGATCATCATAACGCAGAACGTCGATGGCCTTCATGAGAAAGCAGGCTCTCGCGGTGTGCTGCACATGCATGG
CGCGCTCTCAGGCGCGCTCTGTGCCGCCTGTGCGCACCGTTGGCCCGCCCCGCTCCAGATGACGCCCGAAACCCGGTGTC
CTGCCTGTGACGCAAACACGGCTCGGCCGGATGTGGTCTGGTTTGGCGAGGTTCCCTATCATATGGAGCAAATCGCAAGC
CATCTCGCAGAGGCCGATCTCTTTGTTGCCATTGGCACCTCGGGTCAGGTCTATCCGGCAGCGGCGTTTGTCCAAGAGGC
GGCCACATACGGCGCGCAGACGCTCGAACTCAACCTCGAACCCTCGGAAGTGAGTGCAGATTTTGCACAGCATCGCTTTG
GCAAGGCCTCCGACGTTGTTCCGATCTGGGTAGAGGACCTCCTGCGGGACGCTGCGTAA

Upstream 100 bases:

>100_bases
TTTTTGGCACTTCAAAATAATATATATAGTTTTGATAGGCACCAGAGCGCAGGTCATCAATGCTGGAACCAATTTGAAAC
CAGAGGGCAAAGATACTGAG

Downstream 100 bases:

>100_bases
AAAAAGGCTCCGGTCATAACCGGAGCCTTGTCTGACATCTCGGGTGTCTCTGCCAAGATCAGTTCGAGTGATCACCGTGC
CCGCTGTGATTTCCGTGGCC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKKIVILTGAGISAESGLGTFRDKGGLWSQHRIEDVATPEGFARDPELVHRFYSARRQHAAEALPNAAHDALARLEERWT
GDVIIITQNVDGLHEKAGSRGVLHMHGALSGALCAACAHRWPAPLQMTPETRCPACDANTARPDVVWFGEVPYHMEQIAS
HLAEADLFVAIGTSGQVYPAAAFVQEAATYGAQTLELNLEPSEVSADFAQHRFGKASDVVPIWVEDLLRDAA

Sequences:

>Translated_232_residues
MKKIVILTGAGISAESGLGTFRDKGGLWSQHRIEDVATPEGFARDPELVHRFYSARRQHAAEALPNAAHDALARLEERWT
GDVIIITQNVDGLHEKAGSRGVLHMHGALSGALCAACAHRWPAPLQMTPETRCPACDANTARPDVVWFGEVPYHMEQIAS
HLAEADLFVAIGTSGQVYPAAAFVQEAATYGAQTLELNLEPSEVSADFAQHRFGKASDVVPIWVEDLLRDAA
>Mature_232_residues
MKKIVILTGAGISAESGLGTFRDKGGLWSQHRIEDVATPEGFARDPELVHRFYSARRQHAAEALPNAAHDALARLEERWT
GDVIIITQNVDGLHEKAGSRGVLHMHGALSGALCAACAHRWPAPLQMTPETRCPACDANTARPDVVWFGEVPYHMEQIAS
HLAEADLFVAIGTSGQVYPAAAFVQEAATYGAQTLELNLEPSEVSADFAQHRFGKASDVVPIWVEDLLRDAA

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=237, Percent_Identity=38.8185654008439, Blast_Score=157, Evalue=6e-39,
Organism=Homo sapiens, GI6912664, Length=248, Percent_Identity=37.0967741935484, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI13787215, Length=236, Percent_Identity=37.7118644067797, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI6912660, Length=211, Percent_Identity=30.8056872037915, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI63054862, Length=206, Percent_Identity=31.0679611650485, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI6912662, Length=261, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI7657575, Length=221, Percent_Identity=27.6018099547511, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI308199517, Length=230, Percent_Identity=53.4782608695652, Blast_Score=244, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17541892, Length=198, Percent_Identity=28.7878787878788, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17567771, Length=125, Percent_Identity=36.8, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17137536, Length=221, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571445, Length=204, Percent_Identity=32.3529411764706, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25113; Mature: 25113

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVILTGAGISAESGLGTFRDKGGLWSQHRIEDVATPEGFARDPELVHRFYSARRQHA
CCEEEEEECCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AEALPNAAHDALARLEERWTGDVIIITQNVDGLHEKAGSRGVLHMHGALSGALCAACAHR
HHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHC
WPAPLQMTPETRCPACDANTARPDVVWFGEVPYHMEQIASHLAEADLFVAIGTSGQVYPA
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCHHH
AAFVQEAATYGAQTLELNLEPSEVSADFAQHRFGKASDVVPIWVEDLLRDAA
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIVILTGAGISAESGLGTFRDKGGLWSQHRIEDVATPEGFARDPELVHRFYSARRQHA
CCEEEEEECCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AEALPNAAHDALARLEERWTGDVIIITQNVDGLHEKAGSRGVLHMHGALSGALCAACAHR
HHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHC
WPAPLQMTPETRCPACDANTARPDVVWFGEVPYHMEQIASHLAEADLFVAIGTSGQVYPA
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCHHH
AAFVQEAATYGAQTLELNLEPSEVSADFAQHRFGKASDVVPIWVEDLLRDAA
HHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]