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The map label for this gene is sat/cysC [H]

Identifier: 99080453

GI number: 99080453

Start: 650528

End: 652246

Strand: Reverse

Name: sat/cysC [H]

Synonym: TM1040_0612

Alternate gene names: 99080453

Gene position: 652246-650528 (Counterclockwise)

Preceding gene: 99080454

Following gene: 99080451

Centisome position: 20.38

GC content: 59.92

Gene sequence:

>1719_bases
ATGCCAATGTCGAGTAATTTGGCTCCGATCCCGGAGCTGTATGTGTCATATGATTCCGCGCAGAAACTCAAGGTCGAAGC
GGCGGATCTGACCAGCCACGACCTCACCCCGCGCCAGATCTGCGACCTGGAACTGTTGATGAACGGCGGGTTCAACCCGC
TCAAGGGCTTCCTCTCCGAAGAGGACTACAACGGCGTCGTGGAAAACATGCGCCTTGCGGATGGCCAACTTTGGCCGATG
CCGATCACCCTTGATGTCACCGAAGATTTTGCCGCCTCCATCGAGATCGGTCAGGATATTGCCCTGCGCGATCAGGAAGG
CGTGATCCTGGCGACCATGACCGTCACCGACCGTTGGGAGCCCAACAAGTCCCGCGAGGCTGAAAAGGTCTTTGGTGCTG
ATGACGAGGCGCATCCGGCGGTGAATTACCTGCACAACACTGCAGGCAAAATCTACCTCGGCGGACCGGTGACCGGCATC
CAGCAACCCGTCCACTATGACTTCCGCGCCCGTCGCGATACTCCCAACGAGCTGCGCGCCTATTTCCGCAAGCTCGGCTG
GCGCAAGGTTGTGGCCTTCCAGACTCGTAACCCGTTGCACCGCGCCCACCAGGAGCTGACCTTCCGCGCCGCGCGTGAAG
CACAGGCCAACCTGCTCATCCATCCTGTTGTCGGCATGACCAAACCAGGCGATGTGGACCACTTCACCCGCGTGCGCTGC
TACGAGGCGGTGCTGGACAAATACCCGGCGGCCACCACCTCCATGTCGCTCTTGAACCTTGCGATGCGCATGGCTGGCCC
GCGTGAGGCCGTCTGGCATGGTATCATCCGCAAGAACCACGGCTGCACACATTTCATCGTGGGCCGCGATCACGCAGGCC
CGGGCAAGAACTCTGCCGGTGAAGATTTCTATGGCCCCTATGACGCGCAGGACCTGTTCCGCGCCCATCAGGAAGAAATC
GGCATCGAAATGGTCGACTTCAAGCACATGGTCTATGTGCAGGAGCGGGCCCAGTACGAGCCCGCCGACGAAATTGCCGA
CAAGGATGACGTCACCATCCTCAACATCTCCGGCACCGAATTGCGCCGCCGTCTGGCCGAAGGTCTGGAAATCCCCGAGT
GGTTCTCCTTCCCCGAGGTTGTGGCCGAGCTGCGCAAAACCAAGCCGCCGCGCGCGCAGCAGGGCTTTACCGTCTTCTTC
ACCGGGTTCTCCGGCTCGGGCAAATCCACCATTGCAAACGCCTTGATGGTCAAGCTGATGGAAATGGGTGGCCGTCCGGT
CACGCTTCTGGATGGGGACATCGTGCGCAAGAACCTCAGCTCCGAGCTGGGCTTCTCCAAAGAGCACCGCGATCTCAACA
TCAAGCGCATCGGCTATGTCGCCTCCGAGATCACCAAGAACGGCGGTATCGCCATCTGTGCGCCGATCGCGCCTTATGCA
GCCACCCGTCGTCACGTGCGCGAAGAGATCGAACAGTTCGGCGCCTTCGTTGAGGTTCACGTCGCAACCACGATCGAAGA
ATGCGAACGCCGTGACCGCAAGGGGCTCTACAAGCTCGCACGTGAAGGCAAGATCAAGGAATTCACCGGGATTTCCGATC
CCTACGACGTGCCGCAGAACCCTGAACTTCGCGTTGAGACCGAGAATGTCGAAGTCGACAACTGCGCCCATCAGGTGCTG
CTGAAGCTTGAGAGCATGGGTCTCATCAACGGCAACTGA

Upstream 100 bases:

>100_bases
CCTTTTTCTTTGGCGGTCCTAGACATTCCTTTGCCCGAGGGAGTATTGCCCGACACAGCGCTGCCATCGCCCGAGCGACG
CAAGACGAGAGAGATTTATT

Downstream 100 bases:

>100_bases
TCCTTGTATTCAGAAAAGAACAACGGCCCGTCTTGCGCGGGCCGTTTTTTTGTCTCTGAACATGCGGGCTGTGACGTCGA
GTTCGGGGCTCCCAAGTATA

Product: bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase protein

Products: NA

Alternate protein names: Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenosine-5'-phosphosulfate kinase [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MPMSSNLAPIPELYVSYDSAQKLKVEAADLTSHDLTPRQICDLELLMNGGFNPLKGFLSEEDYNGVVENMRLADGQLWPM
PITLDVTEDFAASIEIGQDIALRDQEGVILATMTVTDRWEPNKSREAEKVFGADDEAHPAVNYLHNTAGKIYLGGPVTGI
QQPVHYDFRARRDTPNELRAYFRKLGWRKVVAFQTRNPLHRAHQELTFRAAREAQANLLIHPVVGMTKPGDVDHFTRVRC
YEAVLDKYPAATTSMSLLNLAMRMAGPREAVWHGIIRKNHGCTHFIVGRDHAGPGKNSAGEDFYGPYDAQDLFRAHQEEI
GIEMVDFKHMVYVQERAQYEPADEIADKDDVTILNISGTELRRRLAEGLEIPEWFSFPEVVAELRKTKPPRAQQGFTVFF
TGFSGSGKSTIANALMVKLMEMGGRPVTLLDGDIVRKNLSSELGFSKEHRDLNIKRIGYVASEITKNGGIAICAPIAPYA
ATRRHVREEIEQFGAFVEVHVATTIEECERRDRKGLYKLAREGKIKEFTGISDPYDVPQNPELRVETENVEVDNCAHQVL
LKLESMGLINGN

Sequences:

>Translated_572_residues
MPMSSNLAPIPELYVSYDSAQKLKVEAADLTSHDLTPRQICDLELLMNGGFNPLKGFLSEEDYNGVVENMRLADGQLWPM
PITLDVTEDFAASIEIGQDIALRDQEGVILATMTVTDRWEPNKSREAEKVFGADDEAHPAVNYLHNTAGKIYLGGPVTGI
QQPVHYDFRARRDTPNELRAYFRKLGWRKVVAFQTRNPLHRAHQELTFRAAREAQANLLIHPVVGMTKPGDVDHFTRVRC
YEAVLDKYPAATTSMSLLNLAMRMAGPREAVWHGIIRKNHGCTHFIVGRDHAGPGKNSAGEDFYGPYDAQDLFRAHQEEI
GIEMVDFKHMVYVQERAQYEPADEIADKDDVTILNISGTELRRRLAEGLEIPEWFSFPEVVAELRKTKPPRAQQGFTVFF
TGFSGSGKSTIANALMVKLMEMGGRPVTLLDGDIVRKNLSSELGFSKEHRDLNIKRIGYVASEITKNGGIAICAPIAPYA
ATRRHVREEIEQFGAFVEVHVATTIEECERRDRKGLYKLAREGKIKEFTGISDPYDVPQNPELRVETENVEVDNCAHQVL
LKLESMGLINGN
>Mature_571_residues
PMSSNLAPIPELYVSYDSAQKLKVEAADLTSHDLTPRQICDLELLMNGGFNPLKGFLSEEDYNGVVENMRLADGQLWPMP
ITLDVTEDFAASIEIGQDIALRDQEGVILATMTVTDRWEPNKSREAEKVFGADDEAHPAVNYLHNTAGKIYLGGPVTGIQ
QPVHYDFRARRDTPNELRAYFRKLGWRKVVAFQTRNPLHRAHQELTFRAAREAQANLLIHPVVGMTKPGDVDHFTRVRCY
EAVLDKYPAATTSMSLLNLAMRMAGPREAVWHGIIRKNHGCTHFIVGRDHAGPGKNSAGEDFYGPYDAQDLFRAHQEEIG
IEMVDFKHMVYVQERAQYEPADEIADKDDVTILNISGTELRRRLAEGLEIPEWFSFPEVVAELRKTKPPRAQQGFTVFFT
GFSGSGKSTIANALMVKLMEMGGRPVTLLDGDIVRKNLSSELGFSKEHRDLNIKRIGYVASEITKNGGIAICAPIAPYAA
TRRHVREEIEQFGAFVEVHVATTIEECERRDRKGLYKLAREGKIKEFTGISDPYDVPQNPELRVETENVEVDNCAHQVLL
KLESMGLINGN

Specific function: Catalyzes The Synthesis Of Activated Sulfate. [C]

COG id: COG2046

COG function: function code P; ATP sulfurylase (sulfate adenylyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=179, Percent_Identity=41.8994413407821, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI62912492, Length=177, Percent_Identity=41.8079096045198, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI34447231, Length=177, Percent_Identity=41.8079096045198, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1789107, Length=187, Percent_Identity=42.2459893048128, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17542422, Length=177, Percent_Identity=44.6327683615819, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322469, Length=401, Percent_Identity=55.1122194513716, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322852, Length=183, Percent_Identity=43.7158469945355, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI116007838, Length=180, Percent_Identity=42.7777777777778, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667044, Length=180, Percent_Identity=42.7777777777778, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667040, Length=180, Percent_Identity=42.7777777777778, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667032, Length=180, Percent_Identity=42.7777777777778, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667028, Length=180, Percent_Identity=42.7777777777778, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667036, Length=180, Percent_Identity=42.7777777777778, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR015947
- InterPro:   IPR014729
- InterPro:   IPR020792
- InterPro:   IPR002650 [H]

Pfam domain/function: PF01583 APS_kinase; PF01747 ATP-sulfurylase [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 64191; Mature: 64059

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSSNLAPIPELYVSYDSAQKLKVEAADLTSHDLTPRQICDLELLMNGGFNPLKGFLSE
CCCCCCCCCCCHHHEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCC
EDYNGVVENMRLADGQLWPMPITLDVTEDFAASIEIGQDIALRDQEGVILATMTVTDRWE
CCCCHHHHHCEECCCCCCCCEEEEECCCCHHHHHCCCCCEEEECCCCEEEEEEEEECCCC
PNKSREAEKVFGADDEAHPAVNYLHNTAGKIYLGGPVTGIQQPVHYDFRARRDTPNELRA
CCCCCCHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
YFRKLGWRKVVAFQTRNPLHRAHQELTFRAAREAQANLLIHPVVGMTKPGDVDHFTRVRC
HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHH
YEAVLDKYPAATTSMSLLNLAMRMAGPREAVWHGIIRKNHGCTHFIVGRDHAGPGKNSAG
HHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
EDFYGPYDAQDLFRAHQEEIGIEMVDFKHMVYVQERAQYEPADEIADKDDVTILNISGTE
CCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCHHH
LRRRLAEGLEIPEWFSFPEVVAELRKTKPPRAQQGFTVFFTGFSGSGKSTIANALMVKLM
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
EMGGRPVTLLDGDIVRKNLSSELGFSKEHRDLNIKRIGYVASEITKNGGIAICAPIAPYA
HHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHH
ATRRHVREEIEQFGAFVEVHVATTIEECERRDRKGLYKLAREGKIKEFTGISDPYDVPQN
HHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
PELRVETENVEVDNCAHQVLLKLESMGLINGN
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PMSSNLAPIPELYVSYDSAQKLKVEAADLTSHDLTPRQICDLELLMNGGFNPLKGFLSE
CCCCCCCCCCHHHEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCC
EDYNGVVENMRLADGQLWPMPITLDVTEDFAASIEIGQDIALRDQEGVILATMTVTDRWE
CCCCHHHHHCEECCCCCCCCEEEEECCCCHHHHHCCCCCEEEECCCCEEEEEEEEECCCC
PNKSREAEKVFGADDEAHPAVNYLHNTAGKIYLGGPVTGIQQPVHYDFRARRDTPNELRA
CCCCCCHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
YFRKLGWRKVVAFQTRNPLHRAHQELTFRAAREAQANLLIHPVVGMTKPGDVDHFTRVRC
HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHH
YEAVLDKYPAATTSMSLLNLAMRMAGPREAVWHGIIRKNHGCTHFIVGRDHAGPGKNSAG
HHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
EDFYGPYDAQDLFRAHQEEIGIEMVDFKHMVYVQERAQYEPADEIADKDDVTILNISGTE
CCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCHHH
LRRRLAEGLEIPEWFSFPEVVAELRKTKPPRAQQGFTVFFTGFSGSGKSTIANALMVKLM
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
EMGGRPVTLLDGDIVRKNLSSELGFSKEHRDLNIKRIGYVASEITKNGGIAICAPIAPYA
HHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHH
ATRRHVREEIEQFGAFVEVHVATTIEECERRDRKGLYKLAREGKIKEFTGISDPYDVPQN
HHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
PELRVETENVEVDNCAHQVLLKLESMGLINGN
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]