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The map label for this gene is cobV [H]

Identifier: 99080418

GI number: 99080418

Start: 614143

End: 614895

Strand: Reverse

Name: cobV [H]

Synonym: TM1040_0577

Alternate gene names: 99080418

Gene position: 614895-614143 (Counterclockwise)

Preceding gene: 99080420

Following gene: 99080412

Centisome position: 19.21

GC content: 62.02

Gene sequence:

>753_bases
ATGCGAAAAAACGACTTCAAGCCCATTGATTTGCTGCTCGCGCTGGTCTTGCTGACCCGCCTGCCGCTGCCGCATCTCTC
CAAAGAGGTTTTTGCACGCCAAGCGGCTGCAGTCTGGGCCTTCCCCCTGGTTGGGGCCGTCGTGGGGGTGGTGTCAATTT
TTGCAGGCGCCGTGGCGTTGATCTGGTGTCCGCCCTTCGTGGCCGCCGGAATTGCGCTTGCGACACAGGTTCTTGTGAGC
GGCGCCATGCATGAAGACGGTCTCGCAGATATGGCGGATGGGTTCTGGGGCGGCTTTGACCCCAAGCGGCGTCTGGAGAT
CATGAAGGACAGCCATATTGGCACCTATGGTGTGTTGGCACTGCTCTTTGGATTGGGACTGCGCTGGAGCATTCTGGCCG
CACTCTTTGAGGCGGGGGACATCTGGTGCCTGATGGCGCTTGCATCACTGAGCCGCGCAGCGATGCCCGTGATGATGGCC
AGCATGCCCAACGCGCGCGGGCGAGGCCTGTCGCAGTCAGTTGGCCGTCCAGCCTGGCCAGCGACCCTTGTGGGGCTCCT
GATCGCGCTGGTTGTGGGGTTCCTCAGTACCGGCGGCAGCGTGATCTGGGCGGCACTGGTTCTGGGGCCCTTGCTGCTCG
GCTTGCGTCAGCTTGCAATGACAAAAATCGGCGGGCAGACCGGAGACGTTCTGGGCGCCACGCAGCAACTCAGCGAAATC
GCACTTGGTCTCACATTGCTCGCGCTTCTCTGA

Upstream 100 bases:

>100_bases
AAAAGCCTCGTTGCGTCCTACTGCACCGCAAAGGTAAAGCGATGTCAGGACTGTTTACCGCTCTCGCCCCCTGCCGAGAA
GCCCCACCAGAGGCATCGAC

Downstream 100 bases:

>100_bases
AAGGCCCCTCTCGCATCAAAAAAGGCCGCCCAATCGGGCGGCCTTTGATATTGGGATCCGGAAAGATCAGGCAGCGCGGG
AGGTCAGGACCTCATCAACC

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRKNDFKPIDLLLALVLLTRLPLPHLSKEVFARQAAAVWAFPLVGAVVGVVSIFAGAVALIWCPPFVAAGIALATQVLVS
GAMHEDGLADMADGFWGGFDPKRRLEIMKDSHIGTYGVLALLFGLGLRWSILAALFEAGDIWCLMALASLSRAAMPVMMA
SMPNARGRGLSQSVGRPAWPATLVGLLIALVVGFLSTGGSVIWAALVLGPLLLGLRQLAMTKIGGQTGDVLGATQQLSEI
ALGLTLLALL

Sequences:

>Translated_250_residues
MRKNDFKPIDLLLALVLLTRLPLPHLSKEVFARQAAAVWAFPLVGAVVGVVSIFAGAVALIWCPPFVAAGIALATQVLVS
GAMHEDGLADMADGFWGGFDPKRRLEIMKDSHIGTYGVLALLFGLGLRWSILAALFEAGDIWCLMALASLSRAAMPVMMA
SMPNARGRGLSQSVGRPAWPATLVGLLIALVVGFLSTGGSVIWAALVLGPLLLGLRQLAMTKIGGQTGDVLGATQQLSEI
ALGLTLLALL
>Mature_250_residues
MRKNDFKPIDLLLALVLLTRLPLPHLSKEVFARQAAAVWAFPLVGAVVGVVSIFAGAVALIWCPPFVAAGIALATQVLVS
GAMHEDGLADMADGFWGGFDPKRRLEIMKDSHIGTYGVLALLFGLGLRWSILAALFEAGDIWCLMALASLSRAAMPVMMA
SMPNARGRGLSQSVGRPAWPATLVGLLIALVVGFLSTGGSVIWAALVLGPLLLGLRQLAMTKIGGQTGDVLGATQQLSEI
ALGLTLLALL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes Ado-cobalamin 5'-phosphate from Ado-cobinamide-GDP and alpha-ribazole 5'- phosphate [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=239, Percent_Identity=34.3096234309623, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26189; Mature: 26189

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHC
>Mature Secondary Structure
MRKNDFKPIDLLLALVLLTRLPLPHLSKEVFARQAAAVWAFPLVGAVVGVVSIFAGAVAL
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWCPPFVAAGIALATQVLVSGAMHEDGLADMADGFWGGFDPKRRLEIMKDSHIGTYGVLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHH
LLFGLGLRWSILAALFEAGDIWCLMALASLSRAAMPVMMASMPNARGRGLSQSVGRPAWP
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCH
ATLVGLLIALVVGFLSTGGSVIWAALVLGPLLLGLRQLAMTKIGGQTGDVLGATQQLSEI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALGLTLLALL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1917841 [H]