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The map label for this gene is nrdE [H]

Identifier: 99080360

GI number: 99080360

Start: 545281

End: 547413

Strand: Reverse

Name: nrdE [H]

Synonym: TM1040_0519

Alternate gene names: 99080360

Gene position: 547413-545281 (Counterclockwise)

Preceding gene: 99080361

Following gene: 99080359

Centisome position: 17.1

GC content: 56.96

Gene sequence:

>2133_bases
ATGCTTGACGCAAGCGTAACCGCCGATCTCGACTATCACGCGCTGAACGCGATGTTGAACCTCTATGACGACGAGGGCAA
CATCCAGTTCGACGCCGACCGTATGGCGGCCCGGCAGTATTTCCTGCAACACGTGAACCAGAACACCGTGTTCTTTCACT
CGCTGGATGAAAAGCTCGGCTACCTCGTCGAGAAAGGCTATTACGAGCCGGAGGTGCTGGATCAGTATTCCCGCAACTTC
CACCGCAAGATCTGGGATGCGGCCTACGCCAAGAAGTTCCGCTTCCCGTCCTTCCTCGGGGCGTTCAAATACTACACCTC
CTACACGCTCAAGACCCGTGACGGGCAGCGCTATCTCGAGCGCTATGAAGATCGTGTGGTGATGAACGCGCTGACGCTGG
CACGCGGCGATGAAAAGCTCGCGATGAAGCTGATGTCGGAGATCATCGACGGTCGTTTCCAGCCTGCTACGCCAACCTTC
CTCAATGCCGGCAAAAAAGCCCGTGGCGAGTTTGTGTCCTGCTTCCTCTTGCGTCTCGAAGACAACATGGAGTCGATCGG
ACGCGGCATCAACTCGGCGCTGCAGCTCTCCAAACGCGGCGGTGGCGTGGCCTTGATGCTGACCAACATTCGTGAACACG
GCGCCCCGATCAAGGGCATCGAGAACCAGTCTTCGGGCGTCATCCCGGTGATGAAGCTTCTTGAGGACAGCTTTTCCTAC
GCCAACCAGCTGGGCGCACGCCAGGGCGCGGGCGCGGTCTATCTGAACGCGCATCACCCGGATATTCTGCGTTTCTTGGA
TACCAAGCGCGAAAACGCAGATGAGAAAATCCGCATCAAGACGCTCTCGCTTGGCGTGGTGATCCCCGACATCACCTTCC
AGCTCGCCAAGAAAAACGAGGACATGTATCTGTTCTCGCCGCATGACGTGGAAAAGGTCTACGGTCAGGCCTTCTCGGAA
ATCTCGGTCACCGAGAAATATCACGAGATGGTGGACAACCCGCAGATCCGCAAAAAGAAGATCAACGCGCGCGCGTTCTT
CCAGACCATTGCCGAGATCCAGTTCGAGTCCGGCTACCCTTACATCATGTTCGAGGACACGGTGAATGCCGCAAACCCGA
TTGCGGGCCGCATCGCGATGTCGAACCTCTGCTCGGAGATCCTGCAGGTCAACGAGCCGTCCAAATTCAATGACGACCTG
TCCTATGAGCAGATGGGCACGGATATTTCCTGCAACCTTGGCTCGATGAACATCGCCCGTGCGATGGACGGTGGCGATCT
GGCAGGCACTGTGGGCACCGCGATCCGCGCGCTCAATGCAGTGTCGGAAATGTCGAGCATCGACTCCGTACCTTCCATCC
GCAAGGGCAACGAAGAAGGCCACGCAATTGGTCTTGGTCAGATGAACCTGCATGGATACCTCGCCCGCGAGCGCATCCAT
TACGGCAGCGCAGAAGGTGTGGATTTCACCAATATCTACTTCTGCACGGTTCTGTATCACGCGATCAGCGCCTCGAACGC
CTTGGCAAAGGAAAAGAAATCCAAGTTCAAAGGGTTTGAAGACTCGGCCTATGCCGATGGCAGCTTCTTTGAGAAATATG
TCGAGAAGGACTGGCTGCCGGAAACCGACCGGGTGAAAGAGCTGTTTGAGACCGCAGGTGTCGAGATCCCCACCCGCAAG
GACTGGGAAGCGCTGCGCGACAAGGTGATGAAGCACGGGCTTTATAACCGCAACCTTCAGGCGGTGCCGCCCACCGGCTC
GATCAGCTATATCAACTATGCCACCTCCTCGATCCATCCGATCACCGCAAAGATCGAGATCCGCAAGGAAGGCAAGATCG
GTCGCGTCTACTATCCCGCGCCCTATATGACCAATGACAACCTCGAATATTACCGCGATGCCTATGAAATCGGGCCTGAG
GCGATCATCGACACATATGCGGCTGCGACACAGCATGTGGATCAGGGGCTTTCACTGACCCTGTTCTTCCCGGCGGAAGC
ATCGACCCGCGACATCAACCGCGCGCAGATCTACGCGTGGAAGAAGGGGATCAAGACGATCTACTACATCCGCCTGCGCC
AGCAGGCCCTCGAAGGCACCGAAGTCGAAGGCTGCGTGTCCTGTTCGCTGTAA

Upstream 100 bases:

>100_bases
TCCCCGTCCTTTACCGCTTTGAGTTGGCGGGAACCGAAACAGACATCGCCCGCATGCGCGCGGGCCTCGCCCGGTTCTGG
GCGGCACAGGAGCAGCAGAC

Downstream 100 bases:

>100_bases
ATCGGGTGGGGGCTCTGCCCCCGGCCACCTCCGTGGCCTCCCCCGAGGTATTTTGGAAAAGATGAAGGGGCCTTGCCCCC
AAGCTGCTGAGATCGAGTGT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MLDASVTADLDYHALNAMLNLYDDEGNIQFDADRMAARQYFLQHVNQNTVFFHSLDEKLGYLVEKGYYEPEVLDQYSRNF
HRKIWDAAYAKKFRFPSFLGAFKYYTSYTLKTRDGQRYLERYEDRVVMNALTLARGDEKLAMKLMSEIIDGRFQPATPTF
LNAGKKARGEFVSCFLLRLEDNMESIGRGINSALQLSKRGGGVALMLTNIREHGAPIKGIENQSSGVIPVMKLLEDSFSY
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFQLAKKNEDMYLFSPHDVEKVYGQAFSE
ISVTEKYHEMVDNPQIRKKKINARAFFQTIAEIQFESGYPYIMFEDTVNAANPIAGRIAMSNLCSEILQVNEPSKFNDDL
SYEQMGTDISCNLGSMNIARAMDGGDLAGTVGTAIRALNAVSEMSSIDSVPSIRKGNEEGHAIGLGQMNLHGYLARERIH
YGSAEGVDFTNIYFCTVLYHAISASNALAKEKKSKFKGFEDSAYADGSFFEKYVEKDWLPETDRVKELFETAGVEIPTRK
DWEALRDKVMKHGLYNRNLQAVPPTGSISYINYATSSIHPITAKIEIRKEGKIGRVYYPAPYMTNDNLEYYRDAYEIGPE
AIIDTYAAATQHVDQGLSLTLFFPAEASTRDINRAQIYAWKKGIKTIYYIRLRQQALEGTEVEGCVSCSL

Sequences:

>Translated_710_residues
MLDASVTADLDYHALNAMLNLYDDEGNIQFDADRMAARQYFLQHVNQNTVFFHSLDEKLGYLVEKGYYEPEVLDQYSRNF
HRKIWDAAYAKKFRFPSFLGAFKYYTSYTLKTRDGQRYLERYEDRVVMNALTLARGDEKLAMKLMSEIIDGRFQPATPTF
LNAGKKARGEFVSCFLLRLEDNMESIGRGINSALQLSKRGGGVALMLTNIREHGAPIKGIENQSSGVIPVMKLLEDSFSY
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFQLAKKNEDMYLFSPHDVEKVYGQAFSE
ISVTEKYHEMVDNPQIRKKKINARAFFQTIAEIQFESGYPYIMFEDTVNAANPIAGRIAMSNLCSEILQVNEPSKFNDDL
SYEQMGTDISCNLGSMNIARAMDGGDLAGTVGTAIRALNAVSEMSSIDSVPSIRKGNEEGHAIGLGQMNLHGYLARERIH
YGSAEGVDFTNIYFCTVLYHAISASNALAKEKKSKFKGFEDSAYADGSFFEKYVEKDWLPETDRVKELFETAGVEIPTRK
DWEALRDKVMKHGLYNRNLQAVPPTGSISYINYATSSIHPITAKIEIRKEGKIGRVYYPAPYMTNDNLEYYRDAYEIGPE
AIIDTYAAATQHVDQGLSLTLFFPAEASTRDINRAQIYAWKKGIKTIYYIRLRQQALEGTEVEGCVSCSL
>Mature_710_residues
MLDASVTADLDYHALNAMLNLYDDEGNIQFDADRMAARQYFLQHVNQNTVFFHSLDEKLGYLVEKGYYEPEVLDQYSRNF
HRKIWDAAYAKKFRFPSFLGAFKYYTSYTLKTRDGQRYLERYEDRVVMNALTLARGDEKLAMKLMSEIIDGRFQPATPTF
LNAGKKARGEFVSCFLLRLEDNMESIGRGINSALQLSKRGGGVALMLTNIREHGAPIKGIENQSSGVIPVMKLLEDSFSY
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFQLAKKNEDMYLFSPHDVEKVYGQAFSE
ISVTEKYHEMVDNPQIRKKKINARAFFQTIAEIQFESGYPYIMFEDTVNAANPIAGRIAMSNLCSEILQVNEPSKFNDDL
SYEQMGTDISCNLGSMNIARAMDGGDLAGTVGTAIRALNAVSEMSSIDSVPSIRKGNEEGHAIGLGQMNLHGYLARERIH
YGSAEGVDFTNIYFCTVLYHAISASNALAKEKKSKFKGFEDSAYADGSFFEKYVEKDWLPETDRVKELFETAGVEIPTRK
DWEALRDKVMKHGLYNRNLQAVPPTGSISYINYATSSIHPITAKIEIRKEGKIGRVYYPAPYMTNDNLEYYRDAYEIGPE
AIIDTYAAATQHVDQGLSLTLFFPAEASTRDINRAQIYAWKKGIKTIYYIRLRQQALEGTEVEGCVSCSL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=25.609756097561, Blast_Score=195, Evalue=1e-49,
Organism=Escherichia coli, GI1789030, Length=701, Percent_Identity=69.0442225392297, Blast_Score=1033, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=560, Percent_Identity=27.8571428571429, Blast_Score=186, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17555242, Length=671, Percent_Identity=27.123695976155, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6322123, Length=658, Percent_Identity=26.7477203647416, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320914, Length=655, Percent_Identity=26.1068702290076, Blast_Score=190, Evalue=6e-49,
Organism=Drosophila melanogaster, GI17352481, Length=639, Percent_Identity=25.508607198748, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80137; Mature: 80137

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00089 RIBORED_LARGE ; PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDASVTADLDYHALNAMLNLYDDEGNIQFDADRMAARQYFLQHVNQNTVFFHSLDEKLG
CCCCCEECCCHHHHHHHHHHHEECCCCEEECHHHHHHHHHHHHHCCCCEEEEEEHHHHHH
YLVEKGYYEPEVLDQYSRNFHRKIWDAAYAKKFRFPSFLGAFKYYTSYTLKTRDGQRYLE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCHHHHHH
RYEDRVVMNALTLARGDEKLAMKLMSEIIDGRFQPATPTFLNAGKKARGEFVSCFLLRLE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHH
DNMESIGRGINSALQLSKRGGGVALMLTNIREHGAPIKGIENQSSGVIPVMKLLEDSFSY
HCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFQLAKKNE
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHEECCCCC
DMYLFSPHDVEKVYGQAFSEISVTEKYHEMVDNPQIRKKKINARAFFQTIAEIQFESGYP
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCC
YIMFEDTVNAANPIAGRIAMSNLCSEILQVNEPSKFNDDLSYEQMGTDISCNLGSMNIAR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEEECCCCCHHHH
AMDGGDLAGTVGTAIRALNAVSEMSSIDSVPSIRKGNEEGHAIGLGQMNLHGYLARERIH
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCEEEECCCCHHHHHHHHHHC
YGSAEGVDFTNIYFCTVLYHAISASNALAKEKKSKFKGFEDSAYADGSFFEKYVEKDWLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
ETDRVKELFETAGVEIPTRKDWEALRDKVMKHGLYNRNLQAVPPTGSISYINYATSSIHP
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCE
ITAKIEIRKEGKIGRVYYPAPYMTNDNLEYYRDAYEIGPEAIIDTYAAATQHVDQGLSLT
EEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEE
LFFPAEASTRDINRAQIYAWKKGIKTIYYIRLRQQALEGTEVEGCVSCSL
EEECCCCCCCCCCHHHHHHHHHCCCEEEEHEEHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MLDASVTADLDYHALNAMLNLYDDEGNIQFDADRMAARQYFLQHVNQNTVFFHSLDEKLG
CCCCCEECCCHHHHHHHHHHHEECCCCEEECHHHHHHHHHHHHHCCCCEEEEEEHHHHHH
YLVEKGYYEPEVLDQYSRNFHRKIWDAAYAKKFRFPSFLGAFKYYTSYTLKTRDGQRYLE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECCHHHHHH
RYEDRVVMNALTLARGDEKLAMKLMSEIIDGRFQPATPTFLNAGKKARGEFVSCFLLRLE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHH
DNMESIGRGINSALQLSKRGGGVALMLTNIREHGAPIKGIENQSSGVIPVMKLLEDSFSY
HCHHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ANQLGARQGAGAVYLNAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFQLAKKNE
HHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHEECCCCC
DMYLFSPHDVEKVYGQAFSEISVTEKYHEMVDNPQIRKKKINARAFFQTIAEIQFESGYP
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCC
YIMFEDTVNAANPIAGRIAMSNLCSEILQVNEPSKFNDDLSYEQMGTDISCNLGSMNIAR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEEECCCCCHHHH
AMDGGDLAGTVGTAIRALNAVSEMSSIDSVPSIRKGNEEGHAIGLGQMNLHGYLARERIH
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCEEEECCCCHHHHHHHHHHC
YGSAEGVDFTNIYFCTVLYHAISASNALAKEKKSKFKGFEDSAYADGSFFEKYVEKDWLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
ETDRVKELFETAGVEIPTRKDWEALRDKVMKHGLYNRNLQAVPPTGSISYINYATSSIHP
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCE
ITAKIEIRKEGKIGRVYYPAPYMTNDNLEYYRDAYEIGPEAIIDTYAAATQHVDQGLSLT
EEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEE
LFFPAEASTRDINRAQIYAWKKGIKTIYYIRLRQQALEGTEVEGCVSCSL
EEECCCCCCCCCCHHHHHHHHHCCCEEEEHEEHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]