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The map label for this gene is nrdF [H]

Identifier: 99080359

GI number: 99080359

Start: 544203

End: 545219

Strand: Reverse

Name: nrdF [H]

Synonym: TM1040_0518

Alternate gene names: 99080359

Gene position: 545219-544203 (Counterclockwise)

Preceding gene: 99080360

Following gene: 99080348

Centisome position: 17.03

GC content: 57.92

Gene sequence:

>1017_bases
ATGAAGGGGCCTTGCCCCCAAGCTGCTGAGATCGAGTGTATGAAAGATATGACCCAAGCCCGCGCTGTCCCCAAAGCGAT
CAACTGGAACCGGCTTCAGGACGACAAGGATCTCGAGATCTGGAACCGGTTGACCGTAAACTTCTGGTTGCCTGAAAAGG
TGCCGCTGTCCAATGACATCCAGTCCTGGTCCCAGCTCACACCGGACGAGCAGACCCTGACCATTCGGGTGTTCACTGGC
CTCACGCTTCTGGATACAATCCAGAATGCCGTGGGCGCGCCGACCCTGATGCAGGACGCGATCACCCCGCATGAAGAGGC
TGTGCTGAGCAACATCTCCTTCATGGAGGCGGTGCATGCGCGGTCTTATTCCTCGGTGTTTTCGACCCTCTGTCAGACCA
CCGAGGTGGATGAAGCCTTCCGCTGGTCGGCCGAAAACGAGCATCTGCAGGCCAAATCGCGTCTGATCCTCGGCGAATAT
GACGCCACCGCCTCGCCGCTGCGCAAAAAGATCGCCAGCGTGTTCCTTGAGAGCTTCCTGTTCTATTCGGGCTTCTATCT
GCCGATGCACTGGTCGAGCCGCGCGCGCCTGACCAACACCGCAGACCTCATTCGTCTGATCATTCGCGACGAGGCCATTC
ACGGCTATTACATCGGCTACAAGTTCCAGCGCGGCCTGGAGCGCGCCAGCGAGGCTGAGCGCAAGGAGCTGAAGGATTTT
GCCTTTGGTCTGCTGTTTGACCTCTATGATATCGAGGCGCGCTACACAGAGCAGCTCTATGATGGGCTTGGGCTCACAGA
GGATGTGAAGAACTTCCTGCACTATAACGCCAACAAGGCGCTGCAGAACCTTGGCTTTGAGGCCCTGTTCCCGGAAGAGA
CCTGCCGCGTCAATCCGGCAATCATGGCCGCACTGTCGCCGGGGTCGGATGAAAACCACGACTTCTTTTCCGGGTCCGGC
TCAAGCTATGTGATCGGCAAGGCAGTTGCGACCGAGGATGACGACTGGGATTTCTGA

Upstream 100 bases:

>100_bases
GGCACCGAAGTCGAAGGCTGCGTGTCCTGTTCGCTGTAAATCGGGTGGGGGCTCTGCCCCCGGCCACCTCCGTGGCCTCC
CCCGAGGTATTTTGGAAAAG

Downstream 100 bases:

>100_bases
GGGACTGGTCCTCAAAACACGAAAGCCGCCGCGGGGTTCCCGCTGGCGGCTTTTTTGTCTCCTGTGCGGTGGCACGTGAG
GCCACCCTAGAGCAATCCGC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKGPCPQAAEIECMKDMTQARAVPKAINWNRLQDDKDLEIWNRLTVNFWLPEKVPLSNDIQSWSQLTPDEQTLTIRVFTG
LTLLDTIQNAVGAPTLMQDAITPHEEAVLSNISFMEAVHARSYSSVFSTLCQTTEVDEAFRWSAENEHLQAKSRLILGEY
DATASPLRKKIASVFLESFLFYSGFYLPMHWSSRARLTNTADLIRLIIRDEAIHGYYIGYKFQRGLERASEAERKELKDF
AFGLLFDLYDIEARYTEQLYDGLGLTEDVKNFLHYNANKALQNLGFEALFPEETCRVNPAIMAALSPGSDENHDFFSGSG
SSYVIGKAVATEDDDWDF

Sequences:

>Translated_338_residues
MKGPCPQAAEIECMKDMTQARAVPKAINWNRLQDDKDLEIWNRLTVNFWLPEKVPLSNDIQSWSQLTPDEQTLTIRVFTG
LTLLDTIQNAVGAPTLMQDAITPHEEAVLSNISFMEAVHARSYSSVFSTLCQTTEVDEAFRWSAENEHLQAKSRLILGEY
DATASPLRKKIASVFLESFLFYSGFYLPMHWSSRARLTNTADLIRLIIRDEAIHGYYIGYKFQRGLERASEAERKELKDF
AFGLLFDLYDIEARYTEQLYDGLGLTEDVKNFLHYNANKALQNLGFEALFPEETCRVNPAIMAALSPGSDENHDFFSGSG
SSYVIGKAVATEDDDWDF
>Mature_338_residues
MKGPCPQAAEIECMKDMTQARAVPKAINWNRLQDDKDLEIWNRLTVNFWLPEKVPLSNDIQSWSQLTPDEQTLTIRVFTG
LTLLDTIQNAVGAPTLMQDAITPHEEAVLSNISFMEAVHARSYSSVFSTLCQTTEVDEAFRWSAENEHLQAKSRLILGEY
DATASPLRKKIASVFLESFLFYSGFYLPMHWSSRARLTNTADLIRLIIRDEAIHGYYIGYKFQRGLERASEAERKELKDF
AFGLLFDLYDIEARYTEQLYDGLGLTEDVKNFLHYNANKALQNLGFEALFPEETCRVNPAIMAALSPGSDENHDFFSGSG
SSYVIGKAVATEDDDWDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=313, Percent_Identity=71.5654952076677, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1788567, Length=293, Percent_Identity=25.5972696245734, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38387; Mature: 38387

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGPCPQAAEIECMKDMTQARAVPKAINWNRLQDDKDLEIWNRLTVNFWLPEKVPLSNDI
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEECCCCCCCCHHH
QSWSQLTPDEQTLTIRVFTGLTLLDTIQNAVGAPTLMQDAITPHEEAVLSNISFMEAVHA
HHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
RSYSSVFSTLCQTTEVDEAFRWSAENEHLQAKSRLILGEYDATASPLRKKIASVFLESFL
HHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHH
FYSGFYLPMHWSSRARLTNTADLIRLIIRDEAIHGYYIGYKFQRGLERASEAERKELKDF
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AFGLLFDLYDIEARYTEQLYDGLGLTEDVKNFLHYNANKALQNLGFEALFPEETCRVNPA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCEECCHHHHCCCHH
IMAALSPGSDENHDFFSGSGSSYVIGKAVATEDDDWDF
HEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MKGPCPQAAEIECMKDMTQARAVPKAINWNRLQDDKDLEIWNRLTVNFWLPEKVPLSNDI
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEECCCCCCCCHHH
QSWSQLTPDEQTLTIRVFTGLTLLDTIQNAVGAPTLMQDAITPHEEAVLSNISFMEAVHA
HHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
RSYSSVFSTLCQTTEVDEAFRWSAENEHLQAKSRLILGEYDATASPLRKKIASVFLESFL
HHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHH
FYSGFYLPMHWSSRARLTNTADLIRLIIRDEAIHGYYIGYKFQRGLERASEAERKELKDF
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AFGLLFDLYDIEARYTEQLYDGLGLTEDVKNFLHYNANKALQNLGFEALFPEETCRVNPA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCEECCHHHHCCCHH
IMAALSPGSDENHDFFSGSGSSYVIGKAVATEDDDWDF
HEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8195103; 11677609; 2691838; 2548994; 9748343 [H]