Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 99080344

GI number: 99080344

Start: 530303

End: 531805

Strand: Direct

Name: hisS

Synonym: TM1040_0503

Alternate gene names: 99080344

Gene position: 530303-531805 (Clockwise)

Preceding gene: 99080341

Following gene: 99080345

Centisome position: 16.57

GC content: 60.94

Gene sequence:

>1503_bases
ATGGCCAAAGCCAAGAAACAGCCCCGTCCCAAGGCAATTACGCCCAAAGGATTCCGTGACTATTTCGGCGAAGAGGTGAC
CCAGCGCACGCATATGCTGGCCACCATTGCGGAGGTCTATCATCATTACGGGTTTGAGGCGCTTGAGTCTTCTGCCGTTG
AAACGGTCGAGGCTCTGGGCAAGTTCCTCCCCGATGTGGATCGCCCGAATGAGGGCGTCTTTGCCTGGCAAGAGGCCGAG
GACGATGGCAAAGGTGACTGGATGGCGCTGCGCTATGATCTGACAGCGCCTCTGGCGCGGGTTTATGCCCAGCACCGTAA
CGATCTGCCAAGCCCCTATCGCCGCTATGCGATGGGGCCTGTGTGGCGCAACGAGAAGCCCGGCCCCGGTCGGTTCCGTC
AGTTCTATCAATGCGATGCGGATACCGTTGGCACCGCGTCGATGGCAGCAGATGCCGAGATCTGCATGATGCTCTCGGAT
ACGCTGGAAAAGGTCGGCATCCCGCGTGGCGACTATCTGGTGCGCGTCAACAACCGCAAGGTTCTGAACGGTGTTCTCGA
GGCGATGGGCCTGCTCGAGGACGACCCCAAGCGCGACGACGTCCTGCGCACCATCGACAAGTTCGACAAGGTCGGCGAAA
GCGGCGTGCGTGAGCTTCTGGGTAAAGGGCGGCTTGACGCCTCTGGAGCCTATATCGACGGCGTGGGCCTTGAAGATCAT
CAGGCCGAGCCGGTTTTGGCGTTCCTGACTTCGAAGGGCGACACCGTTACCGAGACCATGACCAACCTGCGGGCTGCCGT
AGGGGACAGCAAGGTCGGCCAGGAGGGCATCGCCGAGCTGGAGCTGATGGGGTCGCTCTTTGCCGCTGCGGGCTATGGTG
AAGACCGCATCCTGATCGACCCGTCGATTGTGCGTGGCCTTGGCTACTACACCGGTCCGGTATTTGAGGCGGAGCTGACC
TTTGAAATCTTTGACGAAAAAGGCCGTAAGCGCCAGTTCGGCTCGGTTGCAGGCGGTGGGCGCTATGACGGTTTGGTGAA
ACGTTTCACCGGCCAAGAAGTCCCTGCCGTGGGGCTCTCGATTGGCGTGGACCGCCTGTTGGCGGCGCTGCGCGAAAAGG
GCCGTTTGGCCGCGACCCCGACGGGGCCGGTTGTGGTCACGGTGATGGATCGTGATCGCATGGCGGACTACCAGGCGATG
GTGGCTGAGCTGCGTCAGGCGGGCATTCGCGCCGAGGTCTATCTCGGCAATCCCAAGAACTTTGGCAATCAGTTGAAATA
CGCCGACAAGCGCCATTCGCCGATTGCGGTCATCGAAGGGGGTGACGAAAAGGATCGTGGCGTGGTCCAGATCAAGGACC
TGATCCTCGGCGCCAAGATCGCAGAAAGCGCCACCTTGGAAGAGTGGAAGGAACGCCCCAGCCAGTTTGAAGTGCCCCGC
ACGGAACTGGTTGCCAAGGTGCGTGAGATCCTCGTCAGCCAATCCAGCGATCGGGAGGGCTGA

Upstream 100 bases:

>100_bases
TCTATGGGAGTCCTGAAGCGCCGGAAGGGTGTCGCACCTTGCTCACTGAGGCCCCTTGGGCTAGACGCAAGCGCGAGAAA
CCACCCGCAAGGAGGCCACC

Downstream 100 bases:

>100_bases
GCATGCCCAACAGATCCCAAATCGAAGCCCGCGCGGCACTATTGCGCCAGCGTTTTGAGGTGGCAGGCGGCGTTGTCGTC
GATCCGCCGCTGCTGCAGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAKAKKQPRPKAITPKGFRDYFGEEVTQRTHMLATIAEVYHHYGFEALESSAVETVEALGKFLPDVDRPNEGVFAWQEAE
DDGKGDWMALRYDLTAPLARVYAQHRNDLPSPYRRYAMGPVWRNEKPGPGRFRQFYQCDADTVGTASMAADAEICMMLSD
TLEKVGIPRGDYLVRVNNRKVLNGVLEAMGLLEDDPKRDDVLRTIDKFDKVGESGVRELLGKGRLDASGAYIDGVGLEDH
QAEPVLAFLTSKGDTVTETMTNLRAAVGDSKVGQEGIAELELMGSLFAAAGYGEDRILIDPSIVRGLGYYTGPVFEAELT
FEIFDEKGRKRQFGSVAGGGRYDGLVKRFTGQEVPAVGLSIGVDRLLAALREKGRLAATPTGPVVVTVMDRDRMADYQAM
VAELRQAGIRAEVYLGNPKNFGNQLKYADKRHSPIAVIEGGDEKDRGVVQIKDLILGAKIAESATLEEWKERPSQFEVPR
TELVAKVREILVSQSSDREG

Sequences:

>Translated_500_residues
MAKAKKQPRPKAITPKGFRDYFGEEVTQRTHMLATIAEVYHHYGFEALESSAVETVEALGKFLPDVDRPNEGVFAWQEAE
DDGKGDWMALRYDLTAPLARVYAQHRNDLPSPYRRYAMGPVWRNEKPGPGRFRQFYQCDADTVGTASMAADAEICMMLSD
TLEKVGIPRGDYLVRVNNRKVLNGVLEAMGLLEDDPKRDDVLRTIDKFDKVGESGVRELLGKGRLDASGAYIDGVGLEDH
QAEPVLAFLTSKGDTVTETMTNLRAAVGDSKVGQEGIAELELMGSLFAAAGYGEDRILIDPSIVRGLGYYTGPVFEAELT
FEIFDEKGRKRQFGSVAGGGRYDGLVKRFTGQEVPAVGLSIGVDRLLAALREKGRLAATPTGPVVVTVMDRDRMADYQAM
VAELRQAGIRAEVYLGNPKNFGNQLKYADKRHSPIAVIEGGDEKDRGVVQIKDLILGAKIAESATLEEWKERPSQFEVPR
TELVAKVREILVSQSSDREG
>Mature_499_residues
AKAKKQPRPKAITPKGFRDYFGEEVTQRTHMLATIAEVYHHYGFEALESSAVETVEALGKFLPDVDRPNEGVFAWQEAED
DGKGDWMALRYDLTAPLARVYAQHRNDLPSPYRRYAMGPVWRNEKPGPGRFRQFYQCDADTVGTASMAADAEICMMLSDT
LEKVGIPRGDYLVRVNNRKVLNGVLEAMGLLEDDPKRDDVLRTIDKFDKVGESGVRELLGKGRLDASGAYIDGVGLEDHQ
AEPVLAFLTSKGDTVTETMTNLRAAVGDSKVGQEGIAELELMGSLFAAAGYGEDRILIDPSIVRGLGYYTGPVFEAELTF
EIFDEKGRKRQFGSVAGGGRYDGLVKRFTGQEVPAVGLSIGVDRLLAALREKGRLAATPTGPVVVTVMDRDRMADYQAMV
AELRQAGIRAEVYLGNPKNFGNQLKYADKRHSPIAVIEGGDEKDRGVVQIKDLILGAKIAESATLEEWKERPSQFEVPRT
ELVAKVREILVSQSSDREG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=465, Percent_Identity=31.8279569892473, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=32.9718004338395, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI65287717, Length=482, Percent_Identity=23.2365145228216, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788861, Length=452, Percent_Identity=25.4424778761062, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=30.6521739130435, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71993686, Length=458, Percent_Identity=31.0043668122271, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325290, Length=498, Percent_Identity=30.1204819277108, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24643061, Length=470, Percent_Identity=32.1276595744681, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24643059, Length=483, Percent_Identity=31.2629399585921, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SILST (Q1GJD0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612498.1
- ProteinModelPortal:   Q1GJD0
- SMR:   Q1GJD0
- STRING:   Q1GJD0
- GeneID:   4078249
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0503
- NMPDR:   fig|292414.1.peg.2331
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- PhylomeDB:   Q1GJD0
- ProtClustDB:   PRK00037
- BioCyc:   SSP292414:TM1040_0503-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 55112; Mature: 54981

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKKQPRPKAITPKGFRDYFGEEVTQRTHMLATIAEVYHHYGFEALESSAVETVEALG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
KFLPDVDRPNEGVFAWQEAEDDGKGDWMALRYDLTAPLARVYAQHRNDLPSPYRRYAMGP
HHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCC
VWRNEKPGPGRFRQFYQCDADTVGTASMAADAEICMMLSDTLEKVGIPRGDYLVRVNNRK
CCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHH
VLNGVLEAMGLLEDDPKRDDVLRTIDKFDKVGESGVRELLGKGRLDASGAYIDGVGLEDH
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
QAEPVLAFLTSKGDTVTETMTNLRAAVGDSKVGQEGIAELELMGSLFAAAGYGEDRILID
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEC
PSIVRGLGYYTGPVFEAELTFEIFDEKGRKRQFGSVAGGGRYDGLVKRFTGQEVPAVGLS
HHHHHHCCHHCCCEEEEEEEHEECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHH
IGVDRLLAALREKGRLAATPTGPVVVTVMDRDRMADYQAMVAELRQAGIRAEVYLGNPKN
HHHHHHHHHHHHCCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
FGNQLKYADKRHSPIAVIEGGDEKDRGVVQIKDLILGAKIAESATLEEWKERPSQFEVPR
CCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
TELVAKVREILVSQSSDREG
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKAKKQPRPKAITPKGFRDYFGEEVTQRTHMLATIAEVYHHYGFEALESSAVETVEALG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
KFLPDVDRPNEGVFAWQEAEDDGKGDWMALRYDLTAPLARVYAQHRNDLPSPYRRYAMGP
HHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCC
VWRNEKPGPGRFRQFYQCDADTVGTASMAADAEICMMLSDTLEKVGIPRGDYLVRVNNRK
CCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCHH
VLNGVLEAMGLLEDDPKRDDVLRTIDKFDKVGESGVRELLGKGRLDASGAYIDGVGLEDH
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
QAEPVLAFLTSKGDTVTETMTNLRAAVGDSKVGQEGIAELELMGSLFAAAGYGEDRILID
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEC
PSIVRGLGYYTGPVFEAELTFEIFDEKGRKRQFGSVAGGGRYDGLVKRFTGQEVPAVGLS
HHHHHHCCHHCCCEEEEEEEHEECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHH
IGVDRLLAALREKGRLAATPTGPVVVTVMDRDRMADYQAMVAELRQAGIRAEVYLGNPKN
HHHHHHHHHHHHCCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCC
FGNQLKYADKRHSPIAVIEGGDEKDRGVVQIKDLILGAKIAESATLEEWKERPSQFEVPR
CCCCCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
TELVAKVREILVSQSSDREG
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA