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The map label for this gene is dadA [C]

Identifier: 99080331

GI number: 99080331

Start: 512568

End: 513653

Strand: Reverse

Name: dadA [C]

Synonym: TM1040_0490

Alternate gene names: 99080331

Gene position: 513653-512568 (Counterclockwise)

Preceding gene: 99080332

Following gene: 99080330

Centisome position: 16.05

GC content: 64.0

Gene sequence:

>1086_bases
ATGATAGACACTCTGATCATCGGCGGCGGAGTGGCCGGGCTTGGTGCAGCGGCACATTTGTCGCCATCGCATCAGGTGGT
GGTGCTGGAACGCGAGGAGGTGCTTGGCTACCACGCCTCCGGGCGCTCTGCCGCGATGTTTGAGCAGGACTATGGCCCTC
CTGCCATCAAGGCACTGTCGCGGGCCAGCCGCACCTTTTTCGAGGCCCCCGGCGAGGGGCGCGAGACATATCTCAGCCCG
CGCGGACTGATGCTCGTTGGGCGCGCGGATCAACGCGCAGCGTTTGATGCAGATCTTGCGGAACTCGATCTTCAGGAGAT
TCCCATCAACGATGCGCTGGCACGGGTGCCGATCCTGAACCGGGAGGTCGTCGCCTTTGCTGCGCTTCATGACACGGCAA
CCGACATCGACACCGACCGGTTGATGGGAGACTGGACCAAAGACCTGCGCCGGCAGGGTGGCCAAACGGTCTTGCGACAG
GAGGTGATGACGATCTCGCGCACCGACAAGGGCTGGCGCGTTGAAACCCGCGACACCCAATATGAGGCCCGCCAGATCGT
CAACGCTGCCGGGGCTTGGGCAGACATCATCGCAGAAACCGCCGGGGTCACTCCGATCGGGCTCCAGCCCAAACGCCGCT
CCATCGCGCAACTGCCTGCCCCAGAGGGTCGAGACGTCGCCAACTGGCCTATGCTCATGGGCGTGGGAGAGGCCTGGTAC
GCCAAACCCCAAGCGGGCAAGCTTCTGGTTTCGCCTGCGGATGCTGATCCCCTGCCTCCACAGGACGCCTGGGCGGATGA
CATGGTACTGGCGGAGGGGCTTGCGCGCTACGAGGAGATGGTGACCACGCCCGTTACACGGCTCGAAACCAGCTGGGCCG
GATTGCGCAGTTTCGCTCCCGACGGTGTGCCGGTTATTGGCGAGGATCCGGATCATCCCGGATTCTGGTGGTACGCGGGC
CAAGGTGGCTACGGGTTTCAAAGCGCCCCTGCCTCGGCCCGGCTACTGCGCGACCTGATGACAGGGCAGCGCCCAGAGAT
CGCTCCTCACATCATTGACGCTCTTGATCCTGCGAGGTTGCGCTGA

Upstream 100 bases:

>100_bases
CGCTCAAGGCCACGGAATTTGACATGTCTCCAGAACTTTATGCGCGTATCGAAGCCCTGAGCCCCCGCCCGGCCCCCGCC
ACGGACCGGCTCGAAGAAGC

Downstream 100 bases:

>100_bases
TGCCCTTTCGTTCCGTCCCGGGCCAGGCTTCGGTCGCCGAGCAAAGTGAGGGTGGCAAGCTCTTTGCCCCTTCTGCGGCG
CGTAATCTGGAGCCCCTGCT

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Oxidoreductase; Glycine/D-Amino Acid Oxidase; Oxidoreductase Protein; D-Amino Acid Dehydrogenase Small Subunit Family Protein; Amino Acid Oxidase; FAD-Dependent Oxidoreductase; D-Amino-Acid Dehydrogenase; FAD Dependent Oxidoreductase Family; Oxidase; FAD Dependent Oxidoreductase Domain-Containing Protein; FAD-Dependent Glycerol-3-Phosphate Dehydrogenase Protein; Fad Dependent Oxidoreductase; Glycine Oxidase; D-Amino Acid Dehydrogenase Small Subunit

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIDTLIIGGGVAGLGAAAHLSPSHQVVVLEREEVLGYHASGRSAAMFEQDYGPPAIKALSRASRTFFEAPGEGRETYLSP
RGLMLVGRADQRAAFDADLAELDLQEIPINDALARVPILNREVVAFAALHDTATDIDTDRLMGDWTKDLRRQGGQTVLRQ
EVMTISRTDKGWRVETRDTQYEARQIVNAAGAWADIIAETAGVTPIGLQPKRRSIAQLPAPEGRDVANWPMLMGVGEAWY
AKPQAGKLLVSPADADPLPPQDAWADDMVLAEGLARYEEMVTTPVTRLETSWAGLRSFAPDGVPVIGEDPDHPGFWWYAG
QGGYGFQSAPASARLLRDLMTGQRPEIAPHIIDALDPARLR

Sequences:

>Translated_361_residues
MIDTLIIGGGVAGLGAAAHLSPSHQVVVLEREEVLGYHASGRSAAMFEQDYGPPAIKALSRASRTFFEAPGEGRETYLSP
RGLMLVGRADQRAAFDADLAELDLQEIPINDALARVPILNREVVAFAALHDTATDIDTDRLMGDWTKDLRRQGGQTVLRQ
EVMTISRTDKGWRVETRDTQYEARQIVNAAGAWADIIAETAGVTPIGLQPKRRSIAQLPAPEGRDVANWPMLMGVGEAWY
AKPQAGKLLVSPADADPLPPQDAWADDMVLAEGLARYEEMVTTPVTRLETSWAGLRSFAPDGVPVIGEDPDHPGFWWYAG
QGGYGFQSAPASARLLRDLMTGQRPEIAPHIIDALDPARLR
>Mature_361_residues
MIDTLIIGGGVAGLGAAAHLSPSHQVVVLEREEVLGYHASGRSAAMFEQDYGPPAIKALSRASRTFFEAPGEGRETYLSP
RGLMLVGRADQRAAFDADLAELDLQEIPINDALARVPILNREVVAFAALHDTATDIDTDRLMGDWTKDLRRQGGQTVLRQ
EVMTISRTDKGWRVETRDTQYEARQIVNAAGAWADIIAETAGVTPIGLQPKRRSIAQLPAPEGRDVANWPMLMGVGEAWY
AKPQAGKLLVSPADADPLPPQDAWADDMVLAEGLARYEEMVTTPVTRLETSWAGLRSFAPDGVPVIGEDPDHPGFWWYAG
QGGYGFQSAPASARLLRDLMTGQRPEIAPHIIDALDPARLR

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39234; Mature: 39234

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTLIIGGGVAGLGAAAHLSPSHQVVVLEREEVLGYHASGRSAAMFEQDYGPPAIKALS
CCCEEEECCCHHHCCHHHCCCCCCCEEEEEHHHHHEECCCCCCCCHHHHCCCCHHHHHHH
RASRTFFEAPGEGRETYLSPRGLMLVGRADQRAAFDADLAELDLQEIPINDALARVPILN
HHHHHHHCCCCCCCCEEECCCCEEEEECCCCCHHHCCHHHHCCHHHCCCCHHHHHCCCCC
REVVAFAALHDTATDIDTDRLMGDWTKDLRRQGGQTVLRQEVMTISRTDKGWRVETRDTQ
CHHEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCH
YEARQIVNAAGAWADIIAETAGVTPIGLQPKRRSIAQLPAPEGRDVANWPMLMGVGEAWY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHEECCHHHC
AKPQAGKLLVSPADADPLPPQDAWADDMVLAEGLARYEEMVTTPVTRLETSWAGLRSFAP
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
DGVPVIGEDPDHPGFWWYAGQGGYGFQSAPASARLLRDLMTGQRPEIAPHIIDALDPARL
CCCEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHCC
R
C
>Mature Secondary Structure
MIDTLIIGGGVAGLGAAAHLSPSHQVVVLEREEVLGYHASGRSAAMFEQDYGPPAIKALS
CCCEEEECCCHHHCCHHHCCCCCCCEEEEEHHHHHEECCCCCCCCHHHHCCCCHHHHHHH
RASRTFFEAPGEGRETYLSPRGLMLVGRADQRAAFDADLAELDLQEIPINDALARVPILN
HHHHHHHCCCCCCCCEEECCCCEEEEECCCCCHHHCCHHHHCCHHHCCCCHHHHHCCCCC
REVVAFAALHDTATDIDTDRLMGDWTKDLRRQGGQTVLRQEVMTISRTDKGWRVETRDTQ
CHHEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCH
YEARQIVNAAGAWADIIAETAGVTPIGLQPKRRSIAQLPAPEGRDVANWPMLMGVGEAWY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHEECCHHHC
AKPQAGKLLVSPADADPLPPQDAWADDMVLAEGLARYEEMVTTPVTRLETSWAGLRSFAP
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
DGVPVIGEDPDHPGFWWYAGQGGYGFQSAPASARLLRDLMTGQRPEIAPHIIDALDPARL
CCCEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA