Click here to switch to the map view.

The map label for this gene is glcD [H]

Identifier: 99080324

GI number: 99080324

Start: 505027

End: 506433

Strand: Direct

Name: glcD [H]

Synonym: TM1040_0483

Alternate gene names: 99080324

Gene position: 505027-506433 (Clockwise)

Preceding gene: 99080323

Following gene: 99080325

Centisome position: 15.78

GC content: 59.42

Gene sequence:

>1407_bases
ATGGTTCAGGCCATCACATTGCCCCGTAACGAAGGCGGTATCAAAGCTGCAATCGCCGCGCTGTCTGAGGCGTTTGGCGA
TCGTCTGCAGACCGGGCAGGCCCTTCGTGAGCAGCATGGGCACACGACCACCTGGATCGACAACCAGGCGCCGGATGCAG
TGGTCTTTCCAACATCCTCCGAAGAGGTCTCTGGTATTGTCAAAATATGCGCCGAGTTTGGTGTGCCGATTATCCCCTTC
GGAACGGGCACCTCGTTGGAGGGGCATGTCAACGCCCCCGCTGGTGGCATCTGCGTGGATATGATGCGGATGGATCAGAT
CGTTGCGGTCCACGCGGAGGATCTCGACGTGGTGGTGCAGCCCGGAGTGACCCGCGAGCAGCTCAACACGTATCTGCGCG
ATCAGGGGCTTTTCTTCCCCATTGATCCCGGCGCAAACGCATCACTGGGTGGCATGGTGGCAACGCGGGCTTCTGGCACC
AATGCTGTGCGCTATGGCACCATGAAGGACAATGTTCTGAGCCTTGAAGCGGTGATGGCAGACGGCAGCATCATCCGGAC
CGCGTCTCGGGCCAAGAAGTCCTCGGCGGGGTATGACATGACCCGTCTTCTGGTTGGTTCTGAGGGGACTTTGGGGCTGA
TCACCGAGATCACCCTGAAGCTGCAGGGCATTCCCGAGGCAATCCGCTCGGCGCGCTGTTCGTTCCGTTCGGTGGATGAG
GCCTGTCAGGCTGTGATGATGACCATTCAATACGGCATCCCCGTCGCCCGCATCGAGCTTTTGGATGAAATGAGTGTGCG
CGCCGCCAATCAGCATTCCGGGCTGGATTTGCCTGAGGTGCCGCTGTTGCTCCTTGAATTTCATGGCTCGGACGCCGGCG
TCGTCGAGCAGTCGGATACCTTTGCGCAAATTGCAGAAGAGTTCGGCGGGTTTGACATTGCCGCGACATCGACGCCGGAA
GAACGCAACAAGCTCTGGCAGGCGCGTCACGACATGTATTGGGCCAGCCTTGGGCTGCGCCCCGGTGCCAAGGGGATCTC
GACCGATGTCTGCGTGCCGATTTCCAAACTCGCCGATTGCGTCTCTGCGGCCCGCTCCAAGGCCGAAGAAATGGGGCTGA
TTGCGCCCATCGTGGGACATGTGGGAGATGGCAATTTCCATGCGCTTTTGCTGATCGATATGGAGAGCGCAGAAGAGCGA
GAAAAGGCGGATACCTATGTCAGCTGGCTCAACGATCTGGCGATTTCCATGGAAGGCACCTGCACCGGGGAACACGGCAT
CGGTCAGGGCAAACGCCCCTATCTGGTTCGTGAATTGGGGGAGACCACCCGCTTTATGGCGGCGGTCAAGGCAGCGCTGG
ACCCGGATAATATCCTCAACCCGGGCAAGATCCTGATTGACGGCTAA

Upstream 100 bases:

>100_bases
CTCTGAGCGCGACGGCCTAGAGTGCGCGGTATATTTTGTTAACAAGGCAAATGCTTTGCGAGATCTTGTTGGCGCTGGGA
ATACGAGAGAGGACACCATC

Downstream 100 bases:

>100_bases
CCTCTGGCCGGTCAAAGGCGCGGCATCAGCTGCTCCTTTCTATGGCCCAATTTCTGCCCCATCTTCTTGCTAGCAGGGCT
TTGTGAGAAGGTGGGGTAAG

Product: FAD linked oxidase-like

Products: (R)-acetoin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVQAITLPRNEGGIKAAIAALSEAFGDRLQTGQALREQHGHTTTWIDNQAPDAVVFPTSSEEVSGIVKICAEFGVPIIPF
GTGTSLEGHVNAPAGGICVDMMRMDQIVAVHAEDLDVVVQPGVTREQLNTYLRDQGLFFPIDPGANASLGGMVATRASGT
NAVRYGTMKDNVLSLEAVMADGSIIRTASRAKKSSAGYDMTRLLVGSEGTLGLITEITLKLQGIPEAIRSARCSFRSVDE
ACQAVMMTIQYGIPVARIELLDEMSVRAANQHSGLDLPEVPLLLLEFHGSDAGVVEQSDTFAQIAEEFGGFDIAATSTPE
ERNKLWQARHDMYWASLGLRPGAKGISTDVCVPISKLADCVSAARSKAEEMGLIAPIVGHVGDGNFHALLLIDMESAEER
EKADTYVSWLNDLAISMEGTCTGEHGIGQGKRPYLVRELGETTRFMAAVKAALDPDNILNPGKILIDG

Sequences:

>Translated_468_residues
MVQAITLPRNEGGIKAAIAALSEAFGDRLQTGQALREQHGHTTTWIDNQAPDAVVFPTSSEEVSGIVKICAEFGVPIIPF
GTGTSLEGHVNAPAGGICVDMMRMDQIVAVHAEDLDVVVQPGVTREQLNTYLRDQGLFFPIDPGANASLGGMVATRASGT
NAVRYGTMKDNVLSLEAVMADGSIIRTASRAKKSSAGYDMTRLLVGSEGTLGLITEITLKLQGIPEAIRSARCSFRSVDE
ACQAVMMTIQYGIPVARIELLDEMSVRAANQHSGLDLPEVPLLLLEFHGSDAGVVEQSDTFAQIAEEFGGFDIAATSTPE
ERNKLWQARHDMYWASLGLRPGAKGISTDVCVPISKLADCVSAARSKAEEMGLIAPIVGHVGDGNFHALLLIDMESAEER
EKADTYVSWLNDLAISMEGTCTGEHGIGQGKRPYLVRELGETTRFMAAVKAALDPDNILNPGKILIDG
>Mature_468_residues
MVQAITLPRNEGGIKAAIAALSEAFGDRLQTGQALREQHGHTTTWIDNQAPDAVVFPTSSEEVSGIVKICAEFGVPIIPF
GTGTSLEGHVNAPAGGICVDMMRMDQIVAVHAEDLDVVVQPGVTREQLNTYLRDQGLFFPIDPGANASLGGMVATRASGT
NAVRYGTMKDNVLSLEAVMADGSIIRTASRAKKSSAGYDMTRLLVGSEGTLGLITEITLKLQGIPEAIRSARCSFRSVDE
ACQAVMMTIQYGIPVARIELLDEMSVRAANQHSGLDLPEVPLLLLEFHGSDAGVVEQSDTFAQIAEEFGGFDIAATSTPE
ERNKLWQARHDMYWASLGLRPGAKGISTDVCVPISKLADCVSAARSKAEEMGLIAPIVGHVGDGNFHALLLIDMESAEER
EKADTYVSWLNDLAISMEGTCTGEHGIGQGKRPYLVRELGETTRFMAAVKAALDPDNILNPGKILIDG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=444, Percent_Identity=50.4504504504504, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI37595754, Length=467, Percent_Identity=47.96573875803, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI119964728, Length=418, Percent_Identity=29.9043062200957, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4501993, Length=457, Percent_Identity=24.72647702407, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1789351, Length=419, Percent_Identity=31.980906921241, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787977, Length=499, Percent_Identity=28.4569138276553, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI48994907, Length=174, Percent_Identity=32.7586206896552, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994914, Length=205, Percent_Identity=28.2926829268293, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=461, Percent_Identity=46.2039045553145, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71992381, Length=461, Percent_Identity=44.6854663774403, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71992392, Length=376, Percent_Identity=49.7340425531915, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17534361, Length=476, Percent_Identity=26.2605042016807, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17556096, Length=480, Percent_Identity=25, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=37.7637130801688, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6320023, Length=438, Percent_Identity=29.9086757990868, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6320764, Length=478, Percent_Identity=26.1506276150628, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18921117, Length=422, Percent_Identity=29.6208530805687, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24639277, Length=422, Percent_Identity=29.6208530805687, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24639275, Length=422, Percent_Identity=29.6208530805687, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24653753, Length=455, Percent_Identity=26.1538461538462, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.4

Molecular weight: Translated: 49949; Mature: 49949

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQAITLPRNEGGIKAAIAALSEAFGDRLQTGQALREQHGHTTTWIDNQAPDAVVFPTSS
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCC
EEVSGIVKICAEFGVPIIPFGTGTSLEGHVNAPAGGICVDMMRMDQIVAVHAEDLDVVVQ
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCEEEEC
PGVTREQLNTYLRDQGLFFPIDPGANASLGGMVATRASGTNAVRYGTMKDNVLSLEAVMA
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEEEECC
DGSIIRTASRAKKSSAGYDMTRLLVGSEGTLGLITEITLKLQGIPEAIRSARCSFRSVDE
CCCCEEHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHCCHHHHHH
ACQAVMMTIQYGIPVARIELLDEMSVRAANQHSGLDLPEVPLLLLEFHGSDAGVVEQSDT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEECCHH
FAQIAEEFGGFDIAATSTPEERNKLWQARHDMYWASLGLRPGAKGISTDVCVPISKLADC
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHH
VSAARSKAEEMGLIAPIVGHVGDGNFHALLLIDMESAEEREKADTYVSWLNDLAISMEGT
HHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEECCC
CTGEHGIGQGKRPYLVRELGETTRFMAAVKAALDPDNILNPGKILIDG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure
MVQAITLPRNEGGIKAAIAALSEAFGDRLQTGQALREQHGHTTTWIDNQAPDAVVFPTSS
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCC
EEVSGIVKICAEFGVPIIPFGTGTSLEGHVNAPAGGICVDMMRMDQIVAVHAEDLDVVVQ
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCEEEEC
PGVTREQLNTYLRDQGLFFPIDPGANASLGGMVATRASGTNAVRYGTMKDNVLSLEAVMA
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEEEECC
DGSIIRTASRAKKSSAGYDMTRLLVGSEGTLGLITEITLKLQGIPEAIRSARCSFRSVDE
CCCCEEHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHHCCHHHHHH
ACQAVMMTIQYGIPVARIELLDEMSVRAANQHSGLDLPEVPLLLLEFHGSDAGVVEQSDT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEECCHH
FAQIAEEFGGFDIAATSTPEERNKLWQARHDMYWASLGLRPGAKGISTDVCVPISKLADC
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHH
VSAARSKAEEMGLIAPIVGHVGDGNFHALLLIDMESAEEREKADTYVSWLNDLAISMEGT
HHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEECCC
CTGEHGIGQGKRPYLVRELGETTRFMAAVKAALDPDNILNPGKILIDG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R,R)-butane-2,3-diol; NAD+

Specific reaction: (R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]