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The map label for this gene is gyaR [H]

Identifier: 99080321

GI number: 99080321

Start: 501938

End: 502924

Strand: Direct

Name: gyaR [H]

Synonym: TM1040_0480

Alternate gene names: 99080321

Gene position: 501938-502924 (Clockwise)

Preceding gene: 99080319

Following gene: 99080323

Centisome position: 15.68

GC content: 62.61

Gene sequence:

>987_bases
GTGCCCAGAGAACGCCTGAGTGTTGTTGTTACGCGACGGTTGCCAGAGCCGGTGGAAACCCGCCTGAGCGAATTGTTCGA
CGTGCGGTTGCGCACCGACGACACCCCAATGACCCGCACAGAGCTGGCCGCCGCCATGAAAGAGGCGGATGTGCTGGTAC
CGACGGTCACCGACACGATTGACGCAGGCCTTCTGGGGCAGGCGGGCGAGCGGCTGCGGCTGATCGCCAATTATGGCGCG
GGTGTCGACAATATCGACGTGGCCACTGCGCGCCAACGGGGCATTCTGGTCTCCAACACCCCCGGCGTGCTGACCGACGA
CACGGCCGATATGACGATGGCGCTGATCATGGCTGTCGTGCGCCGCATTCCCGAGGGTCTTGCGATCATGCAGCGCGGCG
ATTGGGAGGGCTGGTCGCCCACGGCCATGCTTGGCGGGCGTCTTGCAGGGCGCCGACTTGGCATTCTGGGCATGGGCCGC
ATCGGTCAGGCCGTCGCACGCCGTGCCCGCGCTTTTGGCATGCAGGTGCATTACAACAATCGCCGGCGCCTGCGTCCCGA
GGTCGAGGAAGAGCTCGAAGCAACATGGTGGGAAAGCCTTGATCAGATGGTGGCCCGCATGGATGTGATTTCGATCCACT
GTCCCTCGACGCCTTCGACCTTTCACTTGATGAACGCGCGGCGCCTGAAGCTGCTGAAGCCCGATGCGGTGATCGTCAAC
ACCTCGCGCGGGGAGGTGATCGACGAAAACGCTCTGACGCGCGGCCTGCGCGCGGGCGAAATCGCGGGCTGTGGGCTTGA
TGTCTACGAACATGGTACCACCGTTAATCCGCGCCTGCGCGAGCTGCCCAATGTGGTGCTCCTGCCTCATATGGGGTCTG
CTACGATCGAGGGGCGGATCGAAATGGGCGAGAAGGTTCTATTGAATATCAAGACATTTGAAGATGGGCATCGCCCACCG
GATCAGGTGGTGCCTGCCATGTTGTAA

Upstream 100 bases:

>100_bases
CGCAATGCAAGCGCGGGCGGAAGGGTGCTTGTACCCGCAATTATCTTGCGACACGATGTCATGGAATGGAACGACTTGCA
TAGCCCGAGGGAGAAGGCAC

Downstream 100 bases:

>100_bases
GCGCAGAGTCCGGTGCAACGCGGGCAGCGCGATTTGGGAGGAAAGATATGAGACCATTATTTTTAGGCGCGGCGCTCTGT
GCGGCGCTGGCGCATCCTTT

Product: glycolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPRERLSVVVTRRLPEPVETRLSELFDVRLRTDDTPMTRTELAAAMKEADVLVPTVTDTIDAGLLGQAGERLRLIANYGA
GVDNIDVATARQRGILVSNTPGVLTDDTADMTMALIMAVVRRIPEGLAIMQRGDWEGWSPTAMLGGRLAGRRLGILGMGR
IGQAVARRARAFGMQVHYNNRRRLRPEVEEELEATWWESLDQMVARMDVISIHCPSTPSTFHLMNARRLKLLKPDAVIVN
TSRGEVIDENALTRGLRAGEIAGCGLDVYEHGTTVNPRLRELPNVVLLPHMGSATIEGRIEMGEKVLLNIKTFEDGHRPP
DQVVPAML

Sequences:

>Translated_328_residues
MPRERLSVVVTRRLPEPVETRLSELFDVRLRTDDTPMTRTELAAAMKEADVLVPTVTDTIDAGLLGQAGERLRLIANYGA
GVDNIDVATARQRGILVSNTPGVLTDDTADMTMALIMAVVRRIPEGLAIMQRGDWEGWSPTAMLGGRLAGRRLGILGMGR
IGQAVARRARAFGMQVHYNNRRRLRPEVEEELEATWWESLDQMVARMDVISIHCPSTPSTFHLMNARRLKLLKPDAVIVN
TSRGEVIDENALTRGLRAGEIAGCGLDVYEHGTTVNPRLRELPNVVLLPHMGSATIEGRIEMGEKVLLNIKTFEDGHRPP
DQVVPAML
>Mature_327_residues
PRERLSVVVTRRLPEPVETRLSELFDVRLRTDDTPMTRTELAAAMKEADVLVPTVTDTIDAGLLGQAGERLRLIANYGAG
VDNIDVATARQRGILVSNTPGVLTDDTADMTMALIMAVVRRIPEGLAIMQRGDWEGWSPTAMLGGRLAGRRLGILGMGRI
GQAVARRARAFGMQVHYNNRRRLRPEVEEELEATWWESLDQMVARMDVISIHCPSTPSTFHLMNARRLKLLKPDAVIVNT
SRGEVIDENALTRGLRAGEIAGCGLDVYEHGTTVNPRLRELPNVVLLPHMGSATIEGRIEMGEKVLLNIKTFEDGHRPPD
QVVPAML

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=304, Percent_Identity=35.8552631578947, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI23308577, Length=281, Percent_Identity=34.5195729537367, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI61743967, Length=259, Percent_Identity=34.3629343629344, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4557497, Length=259, Percent_Identity=34.3629343629344, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI145580578, Length=246, Percent_Identity=32.520325203252, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4557499, Length=246, Percent_Identity=32.520325203252, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI145580575, Length=246, Percent_Identity=32.520325203252, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI87082289, Length=306, Percent_Identity=36.6013071895425, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1789279, Length=257, Percent_Identity=31.5175097276265, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787645, Length=339, Percent_Identity=26.8436578171091, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788660, Length=289, Percent_Identity=26.2975778546713, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=291, Percent_Identity=34.3642611683849, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147481, Length=241, Percent_Identity=33.6099585062241, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=241, Percent_Identity=36.5145228215768, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=253, Percent_Identity=28.8537549407115, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=253, Percent_Identity=28.0632411067194, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=329, Percent_Identity=25.531914893617, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=261, Percent_Identity=26.0536398467433, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325144, Length=166, Percent_Identity=31.9277108433735, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571528, Length=278, Percent_Identity=39.2086330935252, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45552429, Length=283, Percent_Identity=32.8621908127208, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24585514, Length=283, Percent_Identity=32.8621908127208, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI28574282, Length=283, Percent_Identity=32.8621908127208, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45551003, Length=283, Percent_Identity=32.8621908127208, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI28574284, Length=283, Percent_Identity=32.8621908127208, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI28574286, Length=289, Percent_Identity=31.4878892733564, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24585516, Length=274, Percent_Identity=29.1970802919708, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI62472511, Length=247, Percent_Identity=34.412955465587, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24646446, Length=226, Percent_Identity=36.283185840708, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646448, Length=226, Percent_Identity=36.283185840708, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646452, Length=226, Percent_Identity=36.283185840708, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646450, Length=226, Percent_Identity=36.283185840708, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19921140, Length=309, Percent_Identity=30.7443365695793, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 36248; Mature: 36116

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRERLSVVVTRRLPEPVETRLSELFDVRLRTDDTPMTRTELAAAMKEADVLVPTVTDTI
CCHHHHEEHEECCCCCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCEEEECHHHHH
DAGLLGQAGERLRLIANYGAGVDNIDVATARQRGILVSNTPGVLTDDTADMTMALIMAVV
CCCHHCCCCCEEEEEEECCCCCCCCEEHHHHHCCEEEECCCCCEECCHHHHHHHHHHHHH
RRIPEGLAIMQRGDWEGWSPTAMLGGRLAGRRLGILGMGRIGQAVARRARAFGMQVHYNN
HHCCCCCEEEECCCCCCCCCHHHHCCCCCCCEECEEECCHHHHHHHHHHHHHCEEEEECC
RRRLRPEVEEELEATWWESLDQMVARMDVISIHCPSTPSTFHLMNARRLKLLKPDAVIVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCEEEEECCCEEEEE
TSRGEVIDENALTRGLRAGEIAGCGLDVYEHGTTVNPRLRELPNVVLLPHMGSATIEGRI
CCCCCEECCHHHHCCCCCCCCCCCCCCHHHCCCCCCCHHHHCCCEEEEECCCCCEEECCE
EMGEKVLLNIKTFEDGHRPPDQVVPAML
ECCCEEEEEEEECCCCCCCHHHHCCCCC
>Mature Secondary Structure 
PRERLSVVVTRRLPEPVETRLSELFDVRLRTDDTPMTRTELAAAMKEADVLVPTVTDTI
CHHHHEEHEECCCCCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCEEEECHHHHH
DAGLLGQAGERLRLIANYGAGVDNIDVATARQRGILVSNTPGVLTDDTADMTMALIMAVV
CCCHHCCCCCEEEEEEECCCCCCCCEEHHHHHCCEEEECCCCCEECCHHHHHHHHHHHHH
RRIPEGLAIMQRGDWEGWSPTAMLGGRLAGRRLGILGMGRIGQAVARRARAFGMQVHYNN
HHCCCCCEEEECCCCCCCCCHHHHCCCCCCCEECEEECCHHHHHHHHHHHHHCEEEEECC
RRRLRPEVEEELEATWWESLDQMVARMDVISIHCPSTPSTFHLMNARRLKLLKPDAVIVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCEEEEECCCEEEEE
TSRGEVIDENALTRGLRAGEIAGCGLDVYEHGTTVNPRLRELPNVVLLPHMGSATIEGRI
CCCCCEECCHHHHCCCCCCCCCCCCCCHHHCCCCCCCHHHHCCCEEEEECCCCCEEECCE
EMGEKVLLNIKTFEDGHRPPDQVVPAML
ECCCEEEEEEEECCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA