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The map label for this gene is tcyA [H]

Identifier: 99080304

GI number: 99080304

Start: 481430

End: 482221

Strand: Direct

Name: tcyA [H]

Synonym: TM1040_0463

Alternate gene names: 99080304

Gene position: 481430-482221 (Clockwise)

Preceding gene: 99080303

Following gene: 99080305

Centisome position: 15.04

GC content: 59.47

Gene sequence:

>792_bases
ATGACCCGCACTTTTGCCCTGACTGCCCTTGCAACGGCTGTCTTTGCCACCGGCGCCCTGGCTGCCGATCTGCCGGATCT
TGAAGGGCGCGAGGTCGTGTTTGCGACCGAAAACACCTATCCGCCTCTGCAGTTCATCGACCCCAACAGCGGCGAGGCAA
TTGGTTGGGAATATGATGCAGTGGCCGAGATCGCCAAGCGCATCAACATCACCGTGGTCTACGAGAATTCGAGCTGGGAT
GCGATGATCCCCGCGATCACCGCAAGCCAGTATGATGTCGCCATGAATGGCATCACCATTCGCGATGACCGCAAGGAAAA
GGTCGACTTCTCCGAGAGCTACCTGACCTCGCAGATGCGCATGATCGTTGCGGGCGACGAAGACCGGTTCGAGGACGCAG
CAGGCTTTGCCGCGGATGACGACCTCCTGGCGGCAGCGCAGCCCGGCACCACGCCCTTCTATGTCACCGTCTATGATGTG
CTGGATGGTGATGAAGCAAACCCGCGGATCAAACTGTTTGAAACCTTTGGCGCCTCGCTGCAGGCGCTGCGCGCAGGTGA
TGTGGATCTCGCTCTGTCCGACAGCACCGCCGCAAGTGGCGTTGTTGCGGCCTCGGACGGTGCACTGAAAATCGTTGGCG
AGCCGCTCGGCACCGAGGACTTTGGCTTTATCTTCCCCAAAGGCAGCGATCTGGTGGAACCCGTCAATGCCGCGCTTGCT
GCGATGAAGGCCGATGGCACGCTGGATGCGCTCAACAAGAAGTGGTTCCTCGACTACAAGATGGGCCAATAA

Upstream 100 bases:

>100_bases
TCTATGGTGAAACCCGCCGGATTGCGTTGAAAGCGCGCGCCGATTGTCCGATTTGCGGACCCAATGCAGGCACCCCGGCC
CCGACAGGAGAAAACTGAAT

Downstream 100 bases:

>100_bases
GCGCCTTTCCTTCTGAACTCCAATACCGCCCCGGCCAAACACAGGCCGGGGTTTGTCGTGACAGGGCAAGCACATGACCA
ATCGCCACAAGCGCGAAGAC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTRTFALTALATAVFATGALAADLPDLEGREVVFATENTYPPLQFIDPNSGEAIGWEYDAVAEIAKRINITVVYENSSWD
AMIPAITASQYDVAMNGITIRDDRKEKVDFSESYLTSQMRMIVAGDEDRFEDAAGFAADDDLLAAAQPGTTPFYVTVYDV
LDGDEANPRIKLFETFGASLQALRAGDVDLALSDSTAASGVVAASDGALKIVGEPLGTEDFGFIFPKGSDLVEPVNAALA
AMKADGTLDALNKKWFLDYKMGQ

Sequences:

>Translated_263_residues
MTRTFALTALATAVFATGALAADLPDLEGREVVFATENTYPPLQFIDPNSGEAIGWEYDAVAEIAKRINITVVYENSSWD
AMIPAITASQYDVAMNGITIRDDRKEKVDFSESYLTSQMRMIVAGDEDRFEDAAGFAADDDLLAAAQPGTTPFYVTVYDV
LDGDEANPRIKLFETFGASLQALRAGDVDLALSDSTAASGVVAASDGALKIVGEPLGTEDFGFIFPKGSDLVEPVNAALA
AMKADGTLDALNKKWFLDYKMGQ
>Mature_262_residues
TRTFALTALATAVFATGALAADLPDLEGREVVFATENTYPPLQFIDPNSGEAIGWEYDAVAEIAKRINITVVYENSSWDA
MIPAITASQYDVAMNGITIRDDRKEKVDFSESYLTSQMRMIVAGDEDRFEDAAGFAADDDLLAAAQPGTTPFYVTVYDVL
DGDEANPRIKLFETFGASLQALRAGDVDLALSDSTAASGVVAASDGALKIVGEPLGTEDFGFIFPKGSDLVEPVNAALAA
MKADGTLDALNKKWFLDYKMGQ

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=227, Percent_Identity=29.9559471365639, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787031, Length=256, Percent_Identity=29.296875, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788648, Length=264, Percent_Identity=28.030303030303, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787085, Length=266, Percent_Identity=24.4360902255639, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787088, Length=258, Percent_Identity=24.4186046511628, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28167; Mature: 28035

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTFALTALATAVFATGALAADLPDLEGREVVFATENTYPPLQFIDPNSGEAIGWEYDA
CCCEEHHHHHHHHHHHHCHHCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCHHH
VAEIAKRINITVVYENSSWDAMIPAITASQYDVAMNGITIRDDRKEKVDFSESYLTSQMR
HHHHHHHEEEEEEEECCCCCEEEEEEECCCEEEEECCEEEECCCCCCCCCHHHHHHHEEE
MIVAGDEDRFEDAAGFAADDDLLAAAQPGTTPFYVTVYDVLDGDEANPRIKLFETFGASL
EEEECCCHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCCEEEEEHHCCHH
QALRAGDVDLALSDSTAASGVVAASDGALKIVGEPLGTEDFGFIFPKGSDLVEPVNAALA
EEHCCCCEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHH
AMKADGTLDALNKKWFLDYKMGQ
HHCCCCCHHHCCCEEEEEEECCC
>Mature Secondary Structure 
TRTFALTALATAVFATGALAADLPDLEGREVVFATENTYPPLQFIDPNSGEAIGWEYDA
CCEEHHHHHHHHHHHHCHHCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCHHH
VAEIAKRINITVVYENSSWDAMIPAITASQYDVAMNGITIRDDRKEKVDFSESYLTSQMR
HHHHHHHEEEEEEEECCCCCEEEEEEECCCEEEEECCEEEECCCCCCCCCHHHHHHHEEE
MIVAGDEDRFEDAAGFAADDDLLAAAQPGTTPFYVTVYDVLDGDEANPRIKLFETFGASL
EEEECCCHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCCEEEEEHHCCHH
QALRAGDVDLALSDSTAASGVVAASDGALKIVGEPLGTEDFGFIFPKGSDLVEPVNAALA
EEHCCCCEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHH
AMKADGTLDALNKKWFLDYKMGQ
HHCCCCCHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]