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The map label for this gene is gcvT [H]

Identifier: 99080275

GI number: 99080275

Start: 448712

End: 451171

Strand: Reverse

Name: gcvT [H]

Synonym: TM1040_0434

Alternate gene names: 99080275

Gene position: 451171-448712 (Counterclockwise)

Preceding gene: 99080277

Following gene: 99080274

Centisome position: 14.09

GC content: 60.65

Gene sequence:

>2460_bases
ATGCCCTCTTCCCTTCCGACCCAAGCGCAAGTCGTGATCATCGGCGGGGGGTCGATCGGCTGCAATATCGCCTATCACCT
CACGCAACTTGGCATGACAGACGTTGTGATTCTGGAGCGCGACAAACTGACAGCTGGCACAACGTGGCACGCCGCAGGCG
AAATCGTCCCGGCCGTTCTCGGTGCGGAGTGGGAATGCGAACTCTATCTACACGGCCGCGAACTGATTGCTGGCCTTGAG
GCAGAAACAGGACTGGCGACCGGCTGGCGCGAAGTCGGCTACATTCAACCGGCAGACACCGCGGAACGCCTTGAGGAAAT
GCGCCGTGGCGCGGCCTTCATGAACCTTCACGGTTTCGAAATCCACGAGATCTCACCGCGCGAGGCGCAGGAACGCTTTC
CGATTGGCGACTTCAGCAACACGCTTGCCGCCTTCTGGTATCCCAAGGAAGGCCGCACCAACCCGGTGGACACAACGATG
GCGCTGGCGCGCGGCGCGCGCACTCGCGGCGCGCAGATCCTTGAAGATGTCGCTGTGACAGATATCCTCACGCGGCAAGG
ACGCGCTGTTGGTGTACGTACCGATCATGGCGACATCGCTGCCGAGCATGTGGTTATTGCTGCGGGCATGTGGTCGCGTC
AGATCGGCGCAAAGGCTGGCCTTAATCTGCCGCTGCAGGCCGCAGAACACTATTATCTGATCACAGAAGAGATCGAGGGT
ATCCACCGCGATCTGCCACTGCTCGAGGATCCGCACAATTGGTCCTACTACCGAGAAGAAGTTGGCGGCTTGCTGGTCGG
CATGTTCGAACCCGACGCCGCCCCGTGGATGATCGACGCCATCCCGGACAACTTTGCCTTTGGCGAGATTGAACCGGACT
GGGATCGCATGGCCCCTCACCTCGAAGCCGCTTTCAGCCGTGTGCCACTGGTCGCCGAAGCGGGTGTGCGCAAGCTCTTT
TGCGGACCCGAGAGTTTTACCCCAGATCTTGCCCCCCTGATCGGCGAGGCCCCCAGCCTGCGTAATTGCTGGGTTGCCGC
GGGGATGAACTCGCTCGGGATCCTCTATGGACCGGGGGTCGGCAAGACACTGGCGCACTGGATTGTGGAAGGACGCTGCC
CGGTAGATCACACTGCGTTCAACGTTAGCCGGTTTGCCGACGGCACGCATAACACACCCGCCTACCGACGCGCCCGCACC
CCCGAACTCCTCGCCAAGAGCTTCGGCGCGCATTTTCCAAACGACAGCTTCAAAACGGCCCGCGGCCTCAAGCGCTCGCC
ACTCTACGACCGGCTCAGGGCCGCCGGGGCCTATTTCACGGAGAGCCACGGCTGGGAACTGCCTGACTGGTTTGCCCCGA
CCCCAGAGGCGGCGAAGATCGACCGCTATAGCTGGGGCCGGCAGAACTGGTTTGACTGGCACGCCGAAGAACACCGTGCC
GCCCGCGAGGATGCAATCGTGATGGACATGTCCTCGATGGCAAAATTCCTTGTCCAGGGACGTGACGCCTGTACATTGCT
AAATCGCATCAGCTGCAATGACATCGACGTCCCCATCGGCAAGGTGGTCTATACCGCCTGGACCAACCAAGCGGGCGGCT
TTGAAGCTGACCTTACGGTAACCCGCATGGACGTCGACCGCTTCATGGTTGTGGTCGGGGAGAACAGCCACGGCCATACG
GAGACCTGGATGCGGCGCCACATCGGCGCCGATGAATTTGTAACAATCACGGATATGACCGACGGGATCACGCAGATCAA
CCTGCATGGACCCAAGGCCCGGGACATCCTCGCAAAAGTGAGCGCCGCAGACCTCTCGCAAGAGGCCTTTCCCTTCATGA
CTGCGCAACATATTGATGTCGGGCTCTTTCGCATTCACGCGCTCCGTGTCACATATGTCGGCGAGCTGGGCTGGGAACTG
CACGTCCCGAGCCTGCACGCCGTGCAAGTCTATGACCTGCTGATGCAGGCTGGCGCAGACCACGGGCTGCGCAATGCCGG
GATGCAAACACTGTCTTCGCTGCGACTGGAAAAGGCTTATCGCGACTTTGGCGTGGATCTCGACAATACCGACACACCAA
TTGAGGCAGGCCTGGGCTTTGCCGTGAAGCTCGACAAACCCGGCGGCTTCATTGGGCGCGACGCCCTCGCCAAAATAAAG
GCCGCAGGCGCGCCCAAGCGGCGCATGCTGCAATTCCTGCTGAGAGACCCCGAGCCGCTGCTGCATGGTAATGAAATCAT
CTATCTCGACGATAAACCCGTCGGCTACATCCAGGTCGGCGCCTATGGGCATACCCTTCAAGGCGCGGTCGGCATTGGAT
TTGCGGAGATCGAAACGCCACTGACCTCAGAGATCGCCACGTCAGGCGCTTGGTCAATCGACATCGCAGGGACCAAGATC
GCTGCAACCGCTTCGCTCAAACCGCTCTTCGACCCCGCGATGGAGAGAGTGAGAAAGTGA

Upstream 100 bases:

>100_bases
ATCCACTCCACTGAATGCTCATTCAATCAATTTCTTGATTTTGGCTTAGGGTCAAGCTAGCCATTTGATCAAATTAGAGT
TCGCTGCAGGAGTTGAGACT

Downstream 100 bases:

>100_bases
TAACTTTCAATCTCTTAACTTATTCTGGACTGAGGTTTCTGCACTTCACTAGCGCCAACCTTACCAAACGGTAGGCACCG
TGCACGCTCAACTTACCGAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 819; Mature: 818

Protein sequence:

>819_residues
MPSSLPTQAQVVIIGGGSIGCNIAYHLTQLGMTDVVILERDKLTAGTTWHAAGEIVPAVLGAEWECELYLHGRELIAGLE
AETGLATGWREVGYIQPADTAERLEEMRRGAAFMNLHGFEIHEISPREAQERFPIGDFSNTLAAFWYPKEGRTNPVDTTM
ALARGARTRGAQILEDVAVTDILTRQGRAVGVRTDHGDIAAEHVVIAAGMWSRQIGAKAGLNLPLQAAEHYYLITEEIEG
IHRDLPLLEDPHNWSYYREEVGGLLVGMFEPDAAPWMIDAIPDNFAFGEIEPDWDRMAPHLEAAFSRVPLVAEAGVRKLF
CGPESFTPDLAPLIGEAPSLRNCWVAAGMNSLGILYGPGVGKTLAHWIVEGRCPVDHTAFNVSRFADGTHNTPAYRRART
PELLAKSFGAHFPNDSFKTARGLKRSPLYDRLRAAGAYFTESHGWELPDWFAPTPEAAKIDRYSWGRQNWFDWHAEEHRA
AREDAIVMDMSSMAKFLVQGRDACTLLNRISCNDIDVPIGKVVYTAWTNQAGGFEADLTVTRMDVDRFMVVVGENSHGHT
ETWMRRHIGADEFVTITDMTDGITQINLHGPKARDILAKVSAADLSQEAFPFMTAQHIDVGLFRIHALRVTYVGELGWEL
HVPSLHAVQVYDLLMQAGADHGLRNAGMQTLSSLRLEKAYRDFGVDLDNTDTPIEAGLGFAVKLDKPGGFIGRDALAKIK
AAGAPKRRMLQFLLRDPEPLLHGNEIIYLDDKPVGYIQVGAYGHTLQGAVGIGFAEIETPLTSEIATSGAWSIDIAGTKI
AATASLKPLFDPAMERVRK

Sequences:

>Translated_819_residues
MPSSLPTQAQVVIIGGGSIGCNIAYHLTQLGMTDVVILERDKLTAGTTWHAAGEIVPAVLGAEWECELYLHGRELIAGLE
AETGLATGWREVGYIQPADTAERLEEMRRGAAFMNLHGFEIHEISPREAQERFPIGDFSNTLAAFWYPKEGRTNPVDTTM
ALARGARTRGAQILEDVAVTDILTRQGRAVGVRTDHGDIAAEHVVIAAGMWSRQIGAKAGLNLPLQAAEHYYLITEEIEG
IHRDLPLLEDPHNWSYYREEVGGLLVGMFEPDAAPWMIDAIPDNFAFGEIEPDWDRMAPHLEAAFSRVPLVAEAGVRKLF
CGPESFTPDLAPLIGEAPSLRNCWVAAGMNSLGILYGPGVGKTLAHWIVEGRCPVDHTAFNVSRFADGTHNTPAYRRART
PELLAKSFGAHFPNDSFKTARGLKRSPLYDRLRAAGAYFTESHGWELPDWFAPTPEAAKIDRYSWGRQNWFDWHAEEHRA
AREDAIVMDMSSMAKFLVQGRDACTLLNRISCNDIDVPIGKVVYTAWTNQAGGFEADLTVTRMDVDRFMVVVGENSHGHT
ETWMRRHIGADEFVTITDMTDGITQINLHGPKARDILAKVSAADLSQEAFPFMTAQHIDVGLFRIHALRVTYVGELGWEL
HVPSLHAVQVYDLLMQAGADHGLRNAGMQTLSSLRLEKAYRDFGVDLDNTDTPIEAGLGFAVKLDKPGGFIGRDALAKIK
AAGAPKRRMLQFLLRDPEPLLHGNEIIYLDDKPVGYIQVGAYGHTLQGAVGIGFAEIETPLTSEIATSGAWSIDIAGTKI
AATASLKPLFDPAMERVRK
>Mature_818_residues
PSSLPTQAQVVIIGGGSIGCNIAYHLTQLGMTDVVILERDKLTAGTTWHAAGEIVPAVLGAEWECELYLHGRELIAGLEA
ETGLATGWREVGYIQPADTAERLEEMRRGAAFMNLHGFEIHEISPREAQERFPIGDFSNTLAAFWYPKEGRTNPVDTTMA
LARGARTRGAQILEDVAVTDILTRQGRAVGVRTDHGDIAAEHVVIAAGMWSRQIGAKAGLNLPLQAAEHYYLITEEIEGI
HRDLPLLEDPHNWSYYREEVGGLLVGMFEPDAAPWMIDAIPDNFAFGEIEPDWDRMAPHLEAAFSRVPLVAEAGVRKLFC
GPESFTPDLAPLIGEAPSLRNCWVAAGMNSLGILYGPGVGKTLAHWIVEGRCPVDHTAFNVSRFADGTHNTPAYRRARTP
ELLAKSFGAHFPNDSFKTARGLKRSPLYDRLRAAGAYFTESHGWELPDWFAPTPEAAKIDRYSWGRQNWFDWHAEEHRAA
REDAIVMDMSSMAKFLVQGRDACTLLNRISCNDIDVPIGKVVYTAWTNQAGGFEADLTVTRMDVDRFMVVVGENSHGHTE
TWMRRHIGADEFVTITDMTDGITQINLHGPKARDILAKVSAADLSQEAFPFMTAQHIDVGLFRIHALRVTYVGELGWELH
VPSLHAVQVYDLLMQAGADHGLRNAGMQTLSSLRLEKAYRDFGVDLDNTDTPIEAGLGFAVKLDKPGGFIGRDALAKIKA
AGAPKRRMLQFLLRDPEPLLHGNEIIYLDDKPVGYIQVGAYGHTLQGAVGIGFAEIETPLTSEIATSGAWSIDIAGTKIA
ATASLKPLFDPAMERVRK

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=862, Percent_Identity=36.3109048723898, Blast_Score=540, Evalue=1e-153,
Organism=Homo sapiens, GI21361378, Length=862, Percent_Identity=36.3109048723898, Blast_Score=540, Evalue=1e-153,
Organism=Homo sapiens, GI194306651, Length=835, Percent_Identity=33.6526946107784, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI24797151, Length=824, Percent_Identity=33.1310679611651, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI257796258, Length=372, Percent_Identity=25.5376344086022, Blast_Score=94, Evalue=7e-19,
Organism=Homo sapiens, GI44662838, Length=372, Percent_Identity=25.5376344086022, Blast_Score=93, Evalue=1e-18,
Organism=Homo sapiens, GI257796256, Length=303, Percent_Identity=25.7425742574257, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1789272, Length=310, Percent_Identity=26.4516129032258, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32563613, Length=821, Percent_Identity=30.9378806333739, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71994045, Length=843, Percent_Identity=26.9276393831554, Blast_Score=236, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71994052, Length=850, Percent_Identity=26.5882352941176, Blast_Score=233, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17560118, Length=372, Percent_Identity=24.1935483870968, Blast_Score=86, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320222, Length=237, Percent_Identity=24.8945147679325, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20130091, Length=861, Percent_Identity=34.1463414634146, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI28571104, Length=836, Percent_Identity=32.8947368421053, Blast_Score=434, Evalue=1e-121,
Organism=Drosophila melanogaster, GI20129441, Length=352, Percent_Identity=26.7045454545455, Blast_Score=90, Evalue=6e-18,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 90065; Mature: 89934

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSLPTQAQVVIIGGGSIGCNIAYHLTQLGMTDVVILERDKLTAGTTWHAAGEIVPAVL
CCCCCCCCEEEEEEECCCCCCHHEEHHHHCCCCEEEEEECCCCCCCCEECCCCHHHHHHH
GAEWECELYLHGRELIAGLEAETGLATGWREVGYIQPADTAERLEEMRRGAAFMNLHGFE
CCCEEEEEEECCHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCEE
IHEISPREAQERFPIGDFSNTLAAFWYPKEGRTNPVDTTMALARGARTRGAQILEDVAVT
EECCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
DILTRQGRAVGVRTDHGDIAAEHVVIAAGMWSRQIGAKAGLNLPLQAAEHYYLITEEIEG
HHHHHCCCEEEEECCCCCCCCCEEEEEECCHHHHHCCCCCCCCCHHCCCCEEEEEHHHHH
IHRDLPLLEDPHNWSYYREEVGGLLVGMFEPDAAPWMIDAIPDNFAFGEIEPDWDRMAPH
HHHCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHH
LEAAFSRVPLVAEAGVRKLFCGPESFTPDLAPLIGEAPSLRNCWVAAGMNSLGILYGPGV
HHHHHHHCCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCCCCCEEECCCCCCCEEECCCC
GKTLAHWIVEGRCPVDHTAFNVSRFADGTHNTPAYRRARTPELLAKSFGAHFPNDSFKTA
HHHHHHHHHCCCCCCCCCCEEHHHHCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCHHHH
RGLKRSPLYDRLRAAGAYFTESHGWELPDWFAPTPEAAKIDRYSWGRQNWFDWHAEEHRA
CCCCCCCHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
AREDAIVMDMSSMAKFLVQGRDACTLLNRISCNDIDVPIGKVVYTAWTNQAGGFEADLTV
HHCCEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEECCEEE
TRMDVDRFMVVVGENSHGHTETWMRRHIGADEFVTITDMTDGITQINLHGPKARDILAKV
EEEECCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHH
SAADLSQEAFPFMTAQHIDVGLFRIHALRVTYVGELGWELHVPSLHAVQVYDLLMQAGAD
HHHHCCHHHCCCEEHHHCCCEEEEEEEEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCC
HGLRNAGMQTLSSLRLEKAYRDFGVDLDNTDTPIEAGLGFAVKLDKPGGFIGRDALAKIK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHH
AAGAPKRRMLQFLLRDPEPLLHGNEIIYLDDKPVGYIQVGAYGHTLQGAVGIGFAEIETP
HCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCHHCCCHHHHCCC
LTSEIATSGAWSIDIAGTKIAATASLKPLFDPAMERVRK
HHHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PSSLPTQAQVVIIGGGSIGCNIAYHLTQLGMTDVVILERDKLTAGTTWHAAGEIVPAVL
CCCCCCCEEEEEEECCCCCCHHEEHHHHCCCCEEEEEECCCCCCCCEECCCCHHHHHHH
GAEWECELYLHGRELIAGLEAETGLATGWREVGYIQPADTAERLEEMRRGAAFMNLHGFE
CCCEEEEEEECCHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCEE
IHEISPREAQERFPIGDFSNTLAAFWYPKEGRTNPVDTTMALARGARTRGAQILEDVAVT
EECCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
DILTRQGRAVGVRTDHGDIAAEHVVIAAGMWSRQIGAKAGLNLPLQAAEHYYLITEEIEG
HHHHHCCCEEEEECCCCCCCCCEEEEEECCHHHHHCCCCCCCCCHHCCCCEEEEEHHHHH
IHRDLPLLEDPHNWSYYREEVGGLLVGMFEPDAAPWMIDAIPDNFAFGEIEPDWDRMAPH
HHHCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHH
LEAAFSRVPLVAEAGVRKLFCGPESFTPDLAPLIGEAPSLRNCWVAAGMNSLGILYGPGV
HHHHHHHCCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCCCCCEEECCCCCCCEEECCCC
GKTLAHWIVEGRCPVDHTAFNVSRFADGTHNTPAYRRARTPELLAKSFGAHFPNDSFKTA
HHHHHHHHHCCCCCCCCCCEEHHHHCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCHHHH
RGLKRSPLYDRLRAAGAYFTESHGWELPDWFAPTPEAAKIDRYSWGRQNWFDWHAEEHRA
CCCCCCCHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
AREDAIVMDMSSMAKFLVQGRDACTLLNRISCNDIDVPIGKVVYTAWTNQAGGFEADLTV
HHCCEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEECCEEE
TRMDVDRFMVVVGENSHGHTETWMRRHIGADEFVTITDMTDGITQINLHGPKARDILAKV
EEEECCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHH
SAADLSQEAFPFMTAQHIDVGLFRIHALRVTYVGELGWELHVPSLHAVQVYDLLMQAGAD
HHHHCCHHHCCCEEHHHCCCEEEEEEEEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCC
HGLRNAGMQTLSSLRLEKAYRDFGVDLDNTDTPIEAGLGFAVKLDKPGGFIGRDALAKIK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHH
AAGAPKRRMLQFLLRDPEPLLHGNEIIYLDDKPVGYIQVGAYGHTLQGAVGIGFAEIETP
HCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCCEECCHHCCCHHHHCCC
LTSEIATSGAWSIDIAGTKIAATASLKPLFDPAMERVRK
HHHHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA