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The map label for this gene is purR [H]

Identifier: 99080273

GI number: 99080273

Start: 444427

End: 445458

Strand: Reverse

Name: purR [H]

Synonym: TM1040_0432

Alternate gene names: 99080273

Gene position: 445458-444427 (Counterclockwise)

Preceding gene: 99080274

Following gene: 99080272

Centisome position: 13.92

GC content: 58.14

Gene sequence:

>1032_bases
ATGTCAGAGTCGAAAATCCGCAACATGGAAGAGCTGGCCAGCGTCAGCGGCATCTCCCGACCAACACTCTCCAAGTATTT
CAACAACCCCGACAGCGTCCGCGCCTCAACCCGCAAGAAGGTCGAGGCCGCACTGCAAGAGCACCACTATCAGCCCAACA
TCTACGCAATGAATCAGAACAGGAGGCTGACCAAGAACATTGGCATCGTGGTGCCGTATCTTGCGGATCCTTTCTTTGCT
GAGATTGCGCGTGTGATCGAGAATCTCGTCATTCAGGGTGGCTTTCGACCGATCCTGCTCAGCTCTCATGGCGAGACGAG
GCAGGAGGTCGAAAACCTCGACTCGCTGCGCTCGATCCGTCCGGCTGGCGTTCTGCTGGCCCCCCTTGGCCGCCGCTCTG
ACACGGCCGCGGTCGATCGGTTTTGCACCGATGTCCCGACCGTTCTCTTTGACAACAACCTTGATGACCTCGGACAGGCC
TTCTTTGGGCTCGACAACCCGCAAAGCGTTGGTTTAATGGTGAAACATCTCTGCAAAACTGGGGAGCCGCCCGTTTTCTT
TGAGATGAAAACCCCCACCAACCCCAATGCTTACAAACGCCGTCGCGCATATATCGATACCATGGAAGCCTTGGGACTGG
ACCCCAAACTGGTTCAGGTTGAAGGCGAAACCTGGGACTTTGAAGAGATCGGCTACAAAGGCGGCCTTGCGGCCATCCGA
AACCGCGAACTCTGCACAAACACGGTTCTATGCTCCAATGATCGCCTCGCGATCGGCTTTCTGGCTGCCGCCTATGAATG
CGGACTTCGGGTTGGCTGCGGGACCGGCTGCGCGCTGCGCGTTGCCGGCCACGACGACCACCCCTATGCGCGCTTTACAT
GCCCGCCACTCACCACCATCGCGCAGGATTATTCCGCCATCGCAACGCGCAGCGTTGAGGCGATTCTCAACCTCATCGAA
TCGGGAGAGCACCCGGACCTGCGCAACAGCACCCTGTTCGAGGGCACCTTGATCAAACGCGCCTCCGCCTAA

Upstream 100 bases:

>100_bases
TCCAGTAAAGTTTGCGCCCGCAAAGTTTAAGGTGCACAATGCGCCTCAAACCGCACGGCGAAACCTCTGCAATCGGGACC
CAGAATACTCAGGAGGGCAC

Downstream 100 bases:

>100_bases
GCCCTGCGCCAAGGTCTTGAGTTTGCTGCCCTTCGATGACTATTCTTTACGCGCGTAAATTTTTTATTGACGAATTCGCC
TCCCTGCTCCTACCGTAAGG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSESKIRNMEELASVSGISRPTLSKYFNNPDSVRASTRKKVEAALQEHHYQPNIYAMNQNRRLTKNIGIVVPYLADPFFA
EIARVIENLVIQGGFRPILLSSHGETRQEVENLDSLRSIRPAGVLLAPLGRRSDTAAVDRFCTDVPTVLFDNNLDDLGQA
FFGLDNPQSVGLMVKHLCKTGEPPVFFEMKTPTNPNAYKRRRAYIDTMEALGLDPKLVQVEGETWDFEEIGYKGGLAAIR
NRELCTNTVLCSNDRLAIGFLAAAYECGLRVGCGTGCALRVAGHDDHPYARFTCPPLTTIAQDYSAIATRSVEAILNLIE
SGEHPDLRNSTLFEGTLIKRASA

Sequences:

>Translated_343_residues
MSESKIRNMEELASVSGISRPTLSKYFNNPDSVRASTRKKVEAALQEHHYQPNIYAMNQNRRLTKNIGIVVPYLADPFFA
EIARVIENLVIQGGFRPILLSSHGETRQEVENLDSLRSIRPAGVLLAPLGRRSDTAAVDRFCTDVPTVLFDNNLDDLGQA
FFGLDNPQSVGLMVKHLCKTGEPPVFFEMKTPTNPNAYKRRRAYIDTMEALGLDPKLVQVEGETWDFEEIGYKGGLAAIR
NRELCTNTVLCSNDRLAIGFLAAAYECGLRVGCGTGCALRVAGHDDHPYARFTCPPLTTIAQDYSAIATRSVEAILNLIE
SGEHPDLRNSTLFEGTLIKRASA
>Mature_342_residues
SESKIRNMEELASVSGISRPTLSKYFNNPDSVRASTRKKVEAALQEHHYQPNIYAMNQNRRLTKNIGIVVPYLADPFFAE
IARVIENLVIQGGFRPILLSSHGETRQEVENLDSLRSIRPAGVLLAPLGRRSDTAAVDRFCTDVPTVLFDNNLDDLGQAF
FGLDNPQSVGLMVKHLCKTGEPPVFFEMKTPTNPNAYKRRRAYIDTMEALGLDPKLVQVEGETWDFEEIGYKGGLAAIRN
RELCTNTVLCSNDRLAIGFLAAAYECGLRVGCGTGCALRVAGHDDHPYARFTCPPLTTIAQDYSAIATRSVEAILNLIES
GEHPDLRNSTLFEGTLIKRASA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=342, Percent_Identity=27.7777777777778, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=27.2189349112426, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=27.3809523809524, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=25, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI48994940, Length=298, Percent_Identity=26.8456375838926, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789202, Length=340, Percent_Identity=25.8823529411765, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787948, Length=315, Percent_Identity=26.984126984127, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789068, Length=341, Percent_Identity=21.7008797653959, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=21.6300940438871, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37906; Mature: 37774

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESKIRNMEELASVSGISRPTLSKYFNNPDSVRASTRKKVEAALQEHHYQPNIYAMNQN
CCCHHHCCHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
RRLTKNIGIVVPYLADPFFAEIARVIENLVIQGGFRPILLSSHGETRQEVENLDSLRSIR
CEEECCCCEEEHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCC
PAGVLLAPLGRRSDTAAVDRFCTDVPTVLFDNNLDDLGQAFFGLDNPQSVGLMVKHLCKT
CCCEEEECCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHC
GEPPVFFEMKTPTNPNAYKRRRAYIDTMEALGLDPKLVQVEGETWDFEEIGYKGGLAAIR
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCHHHHC
NRELCTNTVLCSNDRLAIGFLAAAYECGLRVGCGTGCALRVAGHDDHPYARFTCPPLTTI
CCCHHCCEEEECCCCEEHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCEEEECCCHHHH
AQDYSAIATRSVEAILNLIESGEHPDLRNSTLFEGTLIKRASA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHCCCC
>Mature Secondary Structure 
SESKIRNMEELASVSGISRPTLSKYFNNPDSVRASTRKKVEAALQEHHYQPNIYAMNQN
CCHHHCCHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
RRLTKNIGIVVPYLADPFFAEIARVIENLVIQGGFRPILLSSHGETRQEVENLDSLRSIR
CEEECCCCEEEHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCC
PAGVLLAPLGRRSDTAAVDRFCTDVPTVLFDNNLDDLGQAFFGLDNPQSVGLMVKHLCKT
CCCEEEECCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHC
GEPPVFFEMKTPTNPNAYKRRRAYIDTMEALGLDPKLVQVEGETWDFEEIGYKGGLAAIR
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCHHHHC
NRELCTNTVLCSNDRLAIGFLAAAYECGLRVGCGTGCALRVAGHDDHPYARFTCPPLTTI
CCCHHCCEEEECCCCEEHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCEEEECCCHHHH
AQDYSAIATRSVEAILNLIESGEHPDLRNSTLFEGTLIKRASA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA