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The map label for this gene is opuD [H]

Identifier: 99080263

GI number: 99080263

Start: 432884

End: 434839

Strand: Direct

Name: opuD [H]

Synonym: TM1040_0422

Alternate gene names: 99080263

Gene position: 432884-434839 (Clockwise)

Preceding gene: 99080260

Following gene: 99080264

Centisome position: 13.52

GC content: 59.61

Gene sequence:

>1956_bases
ATGCCCGTTTTTTGTGCAAAGTTCGCGTACGGGCGCGATGCTGCGGCGCGGCGTGATTGGATCTGCGGCTTTGCGACGTG
GCTCTCAGCTTCGCGACATCCCCCCGAATTTCAGTCCCAGCGGATGCCGCATCGACGAGTTTTCCTGTCGGATCGCAGTT
GGCGCAGGTGGCGCCAAATCGAGACGATCGCGCAATCCACCTGCAGGCTTTTGCTGCGGTGGCAACCAAGATTGCGCCCG
GCACATTCGCCCGAGGTCGCCTTTCCCCAACAGAGAGGACCAACCACCATGCCGCTTAAACCGCCTTTGACGGAGCTTGA
GATCAAGACTGCCGACAGCGGATTCTACAAAGGGTTTACCAAGGATGTGACAATCACTGCCAAGATCCTTGTTGCCGCCC
TGATCCTCTGGGCCGTGGCCTTTCCAAATCAGGCGGCCAGCGTGCTGTCGCACCTAAACGGGGTCATTCTGGCCAGCTTC
AACTACTGGTACATCTACGTGGTCGCCTTTTTTGTGGTGGTGAGCCTGGGGCTTGCGATCTGGCCTGCATCCGGACGTCT
GCGGCTCGGAGCGGAGCATGAAAAGCCAGAGTTCTCGGCCTTCTCCTGGTTCTCCATGATGTTCGGCGCTGGCATGGGAA
TTGGTGTTCTGACCTATGCCACCGGTGAGCCGATCTATCACTTCCAGAACAACCCGGATGTCATCCAGGGTTTTGTCGAA
GGGGCCAGCGCCGAGACGGTGCGCTCTGCCTACAAGTGGTCCTTTTTGCATTGGGGTCTGTCTCCATGGGGGGCCTATGC
GTTGACCGGGCTTGCGCTCGCGTTCTTCGCTTACCGCCGCGGGCTCCCGCTGACCATTCGGTCGGCGCTGACGCCTCTGT
TCGGCGATCGGCTGTCGGGTTTTGCGGGTCATGCCGTCGATGTGGTGGCCGTCATCGCGACCGTGCTGGGGGTGGCGCAA
ACGCTCGGCTTTGGGGTCGAGCAATTCGTCGCGGGGCTGAGCCGCATCGGCTTTGGCGACTGGCTTCTTGTCACAACCGA
TGAAGGAACCCAAAAGGCGTCTGTGGTGGCGATCGTGCTGTCGCTGGTGATCATCATGGGGGCTTCGACGCTGTCGGCCC
TGTCGGGTGTCGGCAAGGGGATCAAATGGCTCTCCAACATCAACATGGGGTTGAGCGTGTTCTTGCTGTCGTTTTTCCTG
ATCTTTGGCTCCACGGTTTTTGGCCTCACGGCGCTGGTGACAGGGATCTATGACTATCTCCTGGCTTTTCCCGCGATGCT
GTTCACCGTCTGGAGCGACAAAGGCACCGAGACCAGCAGCGCACTTGAGAGCTGGCAGGGTGGTTGGACCATTTTCTACT
GGGCTTGGTGGATTGCGTTTGCTCCCTTCGTCGGTTTGTTCCTCGCCCGGATCTCTCGAGGTCGGACGATCCGCGAATAT
GTCTTTGGCGCGATGCTGGTACCATCCGTCATGTGTTTTGTCTGGTTCGCCCTGATTGGCGGCACCGCCATCGATCTGGA
ACTGTCGGGCATCGCAGAGGGTGCAATCCTTGGAACGGGGCTGTCAGACCAGCTCTATGCAACCCTCGCAGTTCTCCTCA
GCGATGGTCTGGCCTGGGTGTTCTCGGTGGTCGTCGCGGTTCTGTTGATGACCTACCTTGTCACCTCGGCGGATTCGGCG
GTGTTGATCATCAACACCATCAACGCCGCAGGTGACGAGGGACCAAAGGCACGTCCTCATATCATCTTCTGGGGCGTCGC
CTTGGCGTTGGTTGTCGGAGCTTTGCTGATCATTGGTGGTCTCGGTGCGATCCAGACCGCGATGATTATCGGCGCGCTGC
CCTTCTCGGTGGTGATGGCGTTGATGGGCGTTGCCTTGATCAAGGCCATCATCCTCGACGGGCTTCGGGGCCGCGCGGGA
CTGCCAGTTACGGCAGATCAGCTCGCGGGCGAGTAG

Upstream 100 bases:

>100_bases
AAGGCCGTGCGTGAACTGTTCCCCAAGCCTAAGGAGCGCCGGGTATCTGGGCAAAGGTCGCGGGGCGTGCTCTGCTTTGA
TATGCTACAAATCTCAACAT

Downstream 100 bases:

>100_bases
TCCGGTCATGTCCAAACGATTGCCCGCGCGACATCCTGCGCGCGGGCAATGACATCCTGCGCGCGGGCAATCCGGGGGCT
TTGCGCGGCACAGGGACATC

Product: BCCT transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MPVFCAKFAYGRDAAARRDWICGFATWLSASRHPPEFQSQRMPHRRVFLSDRSWRRWRQIETIAQSTCRLLLRWQPRLRP
AHSPEVAFPQQRGPTTMPLKPPLTELEIKTADSGFYKGFTKDVTITAKILVAALILWAVAFPNQAASVLSHLNGVILASF
NYWYIYVVAFFVVVSLGLAIWPASGRLRLGAEHEKPEFSAFSWFSMMFGAGMGIGVLTYATGEPIYHFQNNPDVIQGFVE
GASAETVRSAYKWSFLHWGLSPWGAYALTGLALAFFAYRRGLPLTIRSALTPLFGDRLSGFAGHAVDVVAVIATVLGVAQ
TLGFGVEQFVAGLSRIGFGDWLLVTTDEGTQKASVVAIVLSLVIIMGASTLSALSGVGKGIKWLSNINMGLSVFLLSFFL
IFGSTVFGLTALVTGIYDYLLAFPAMLFTVWSDKGTETSSALESWQGGWTIFYWAWWIAFAPFVGLFLARISRGRTIREY
VFGAMLVPSVMCFVWFALIGGTAIDLELSGIAEGAILGTGLSDQLYATLAVLLSDGLAWVFSVVVAVLLMTYLVTSADSA
VLIINTINAAGDEGPKARPHIIFWGVALALVVGALLIIGGLGAIQTAMIIGALPFSVVMALMGVALIKAIILDGLRGRAG
LPVTADQLAGE

Sequences:

>Translated_651_residues
MPVFCAKFAYGRDAAARRDWICGFATWLSASRHPPEFQSQRMPHRRVFLSDRSWRRWRQIETIAQSTCRLLLRWQPRLRP
AHSPEVAFPQQRGPTTMPLKPPLTELEIKTADSGFYKGFTKDVTITAKILVAALILWAVAFPNQAASVLSHLNGVILASF
NYWYIYVVAFFVVVSLGLAIWPASGRLRLGAEHEKPEFSAFSWFSMMFGAGMGIGVLTYATGEPIYHFQNNPDVIQGFVE
GASAETVRSAYKWSFLHWGLSPWGAYALTGLALAFFAYRRGLPLTIRSALTPLFGDRLSGFAGHAVDVVAVIATVLGVAQ
TLGFGVEQFVAGLSRIGFGDWLLVTTDEGTQKASVVAIVLSLVIIMGASTLSALSGVGKGIKWLSNINMGLSVFLLSFFL
IFGSTVFGLTALVTGIYDYLLAFPAMLFTVWSDKGTETSSALESWQGGWTIFYWAWWIAFAPFVGLFLARISRGRTIREY
VFGAMLVPSVMCFVWFALIGGTAIDLELSGIAEGAILGTGLSDQLYATLAVLLSDGLAWVFSVVVAVLLMTYLVTSADSA
VLIINTINAAGDEGPKARPHIIFWGVALALVVGALLIIGGLGAIQTAMIIGALPFSVVMALMGVALIKAIILDGLRGRAG
LPVTADQLAGE
>Mature_650_residues
PVFCAKFAYGRDAAARRDWICGFATWLSASRHPPEFQSQRMPHRRVFLSDRSWRRWRQIETIAQSTCRLLLRWQPRLRPA
HSPEVAFPQQRGPTTMPLKPPLTELEIKTADSGFYKGFTKDVTITAKILVAALILWAVAFPNQAASVLSHLNGVILASFN
YWYIYVVAFFVVVSLGLAIWPASGRLRLGAEHEKPEFSAFSWFSMMFGAGMGIGVLTYATGEPIYHFQNNPDVIQGFVEG
ASAETVRSAYKWSFLHWGLSPWGAYALTGLALAFFAYRRGLPLTIRSALTPLFGDRLSGFAGHAVDVVAVIATVLGVAQT
LGFGVEQFVAGLSRIGFGDWLLVTTDEGTQKASVVAIVLSLVIIMGASTLSALSGVGKGIKWLSNINMGLSVFLLSFFLI
FGSTVFGLTALVTGIYDYLLAFPAMLFTVWSDKGTETSSALESWQGGWTIFYWAWWIAFAPFVGLFLARISRGRTIREYV
FGAMLVPSVMCFVWFALIGGTAIDLELSGIAEGAILGTGLSDQLYATLAVLLSDGLAWVFSVVVAVLLMTYLVTSADSAV
LIINTINAAGDEGPKARPHIIFWGVALALVVGALLIIGGLGAIQTAMIIGALPFSVVMALMGVALIKAIILDGLRGRAGL
PVTADQLAGE

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=496, Percent_Identity=36.6935483870968, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1786224, Length=509, Percent_Identity=27.7013752455796, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1788102, Length=458, Percent_Identity=29.2576419213974, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 70486; Mature: 70355

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFCAKFAYGRDAAARRDWICGFATWLSASRHPPEFQSQRMPHRRVFLSDRSWRRWRQI
CCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEECCCHHHHHHHH
ETIAQSTCRLLLRWQPRLRPAHSPEVAFPQQRGPTTMPLKPPLTELEIKTADSGFYKGFT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHCCCC
KDVTITAKILVAALILWAVAFPNQAASVLSHLNGVILASFNYWYIYVVAFFVVVSLGLAI
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHE
WPASGRLRLGAEHEKPEFSAFSWFSMMFGAGMGIGVLTYATGEPIYHFQNNPDVIQGFVE
ECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHHHC
GASAETVRSAYKWSFLHWGLSPWGAYALTGLALAFFAYRRGLPLTIRSALTPLFGDRLSG
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCC
FAGHAVDVVAVIATVLGVAQTLGFGVEQFVAGLSRIGFGDWLLVTTDEGTQKASVVAIVL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
SLVIIMGASTLSALSGVGKGIKWLSNINMGLSVFLLSFFLIFGSTVFGLTALVTGIYDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFPAMLFTVWSDKGTETSSALESWQGGWTIFYWAWWIAFAPFVGLFLARISRGRTIREY
HHHHHHHHHHHCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VFGAMLVPSVMCFVWFALIGGTAIDLELSGIAEGAILGTGLSDQLYATLAVLLSDGLAWV
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCHHHH
FSVVVAVLLMTYLVTSADSAVLIINTINAAGDEGPKARPHIIFWGVALALVVGALLIIGG
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
LGAIQTAMIIGALPFSVVMALMGVALIKAIILDGLRGRAGLPVTADQLAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
PVFCAKFAYGRDAAARRDWICGFATWLSASRHPPEFQSQRMPHRRVFLSDRSWRRWRQI
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEECCCHHHHHHHH
ETIAQSTCRLLLRWQPRLRPAHSPEVAFPQQRGPTTMPLKPPLTELEIKTADSGFYKGFT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHCCCC
KDVTITAKILVAALILWAVAFPNQAASVLSHLNGVILASFNYWYIYVVAFFVVVSLGLAI
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHE
WPASGRLRLGAEHEKPEFSAFSWFSMMFGAGMGIGVLTYATGEPIYHFQNNPDVIQGFVE
ECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHHHC
GASAETVRSAYKWSFLHWGLSPWGAYALTGLALAFFAYRRGLPLTIRSALTPLFGDRLSG
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCC
FAGHAVDVVAVIATVLGVAQTLGFGVEQFVAGLSRIGFGDWLLVTTDEGTQKASVVAIVL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
SLVIIMGASTLSALSGVGKGIKWLSNINMGLSVFLLSFFLIFGSTVFGLTALVTGIYDYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFPAMLFTVWSDKGTETSSALESWQGGWTIFYWAWWIAFAPFVGLFLARISRGRTIREY
HHHHHHHHHHHCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VFGAMLVPSVMCFVWFALIGGTAIDLELSGIAEGAILGTGLSDQLYATLAVLLSDGLAWV
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCHHHH
FSVVVAVLLMTYLVTSADSAVLIINTINAAGDEGPKARPHIIFWGVALALVVGALLIIGG
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHC
LGAIQTAMIIGALPFSVVMALMGVALIKAIILDGLRGRAGLPVTADQLAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]