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The map label for this gene is zraS [H]

Identifier: 99080247

GI number: 99080247

Start: 416325

End: 418301

Strand: Reverse

Name: zraS [H]

Synonym: TM1040_0406

Alternate gene names: 99080247

Gene position: 418301-416325 (Counterclockwise)

Preceding gene: 99080248

Following gene: 99080237

Centisome position: 13.07

GC content: 60.24

Gene sequence:

>1977_bases
ATGCTGAAATCTGTCCGCTTGCGCCTGCTCATTCTGGCGCTGCTACCGCTTATCGTGTTGATGCCGCTTTTGATGGTGGT
GGCGATGGCGCGTTGGAATGCGGATTACGATAAAATATTGATCGAAAAGGTCGAAAGCGATCTGCGCATCGCCGAGCAAT
ATCTCGGCCACCTTCAGAGCAACTCGGCGCGTGCGCTGCAAGGACTTGCCCAATCAGCCGAGATCGCAGACGTCCTCACG
CGCGACCCAGGTTTAACGCCAGCCTTTCTGGAGCAAAGCCAGGATCGCCTCGGACTCGACTTTCTCTATCTTCTACAAGG
CGATGAGCTGACTGACGCCAAAGAAAAATGGCCGGTCATCTTTCAAGCCGCCGCTGGCCGCATGGTCTCGGAGATCGATA
TTCTCTCGGCCAGCGATCTGGCGGCCCTTTCCCCTGCGCTGGCGGCAGAAGCCCGGATTGAGCTGATCGAGACCGAAGCC
GCCGCCCCCGCCGATCACACCGTGGAGGATCGGGGTATGGTGGTGCACACCGCCGCCCCAGTGCGCGTGAATGGCGAGAT
CCGGGTGTTGGTGGGGGGGATCTTGCTCAACCGCAACCTTGATTTCATCGATACGCTCAACGAACTCGTCTATCTGCAAG
GACAGTACAGCGATGGGCGACAAGGCACCGCCACCCTGTTTTTATCAGACACCCGCGTCTCCACAAATGTGCGCCTCTTC
GAGGACGTGCGTGCGCTTGGCACGCGGGTGTCTGCTGCCGTACGTGACGCGGTGCTCGGCAAGGGGCGCACATGGCTAGA
CAGCGCCTTTGTCGTGAATGATTGGTACATTTCGGGGTATCTGCCGATCAGCGACAGCTTTGGCAAACGGGTCGGCATGC
TCTACGTCGGCTTCCTGGAAGCCCCCTATGAGACCGCAAAACGCGCGGCTTATTGGACGGTGGTTGGGGCATTTGTGCTG
GTGATCGCACTCACCGCGCCGCTGTTCTTGTGGTTGGCCAAAGGGATCTTTGCCCCACTCGAGCAGATGACGCGCACCAT
GGCGAGGGTGGAGCGCGGCGACCTCACCGCCCGCAATCAGACCTCGGCGCGCGGTGACGAGATCGGTCAGGTGGCCAATC
ACCTCAACACATTGCTTGATCAGGTCCAGGAACGCGACCGGCGGCTGCGCAACTGGGCCGGAGAGCTGAACCTGCGCGTC
GAAGAGCGCACGCGAGAGTTGCAAGTCGCAAATGCCAAACTCGAAGAGACCTTTCGCCAGCTGGTGATGAGTGAAAAACT
GGCCTCGATCGGGGAAATCACCGCAGGCGTCGCCCATGAGATCAACAATCCCGTCGCGGTGATCCAGGGCAATGTGGATG
TGATGCGTATGACCCTTGGCGACGCAGCAGAGCCGGTGCGCACCGAGCTCGATCTGATCGACAATCAAGTGATGCGCATC
GGGGCCATCGTGGGCAAACTCTTGCAGTTCGCGCGCCCGTCGGAGTTTGGCACCTTTGCCGAGCATGTGGATGTGGCCGC
CGTGGTCCGCGACTGTCTTGTGTTGGTGGATCACGTCATCTCGAAACAAGAGGTGCGGGTCCTCACACAGTTTGACGCCG
CGCCACACGTCCGCATCGACCCGGGCGAATTGCAACAGGTGGTGATCAATATCATCATCAACGCCAGCCAGGCGATGGAG
GGCAAAGGCACGCTTGCGATCTCTGTTGCGGGCGAGGTACGCGATGCACAGACAGGTGTTGCCCTGCGGATCGCCGATAC
CGGCCCCGGCATCCCCATGGAGCGGATCGATCACATATTCGACCCTTTCTTTACGACAAAACAGGGCGAAGGCACGGGGC
TTGGGCTGTCCATCAGCCAGACCCTCATTCAGCGGGCTGGCGGGCGCATCACGGTGCGCAACAGACGCGCGCGGGGGGCG
GAGTTCCTGATCTGGCTGCCAGAGCCAACTGACGCGGATGACCCGGATCAGAGATAA

Upstream 100 bases:

>100_bases
ATCCAAATCCCTCAGCGCCCGCCTTGCACCCAAGGCGCGTTCCAGCGAGACTGTTCGCACATGGAGCGCCGTCTGGCGGC
GCTGGGAGGGAGCCCCATTC

Downstream 100 bases:

>100_bases
CGCGCTGTTTCGCGCAAGACGCAGGCGGCGCTATAGCCCCCAGCTTGCGCATTTCCTGTCGACGGTCTTGCGCGACACTC
CCAGTCGTCGGGCCGCCTCT

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MLKSVRLRLLILALLPLIVLMPLLMVVAMARWNADYDKILIEKVESDLRIAEQYLGHLQSNSARALQGLAQSAEIADVLT
RDPGLTPAFLEQSQDRLGLDFLYLLQGDELTDAKEKWPVIFQAAAGRMVSEIDILSASDLAALSPALAAEARIELIETEA
AAPADHTVEDRGMVVHTAAPVRVNGEIRVLVGGILLNRNLDFIDTLNELVYLQGQYSDGRQGTATLFLSDTRVSTNVRLF
EDVRALGTRVSAAVRDAVLGKGRTWLDSAFVVNDWYISGYLPISDSFGKRVGMLYVGFLEAPYETAKRAAYWTVVGAFVL
VIALTAPLFLWLAKGIFAPLEQMTRTMARVERGDLTARNQTSARGDEIGQVANHLNTLLDQVQERDRRLRNWAGELNLRV
EERTRELQVANAKLEETFRQLVMSEKLASIGEITAGVAHEINNPVAVIQGNVDVMRMTLGDAAEPVRTELDLIDNQVMRI
GAIVGKLLQFARPSEFGTFAEHVDVAAVVRDCLVLVDHVISKQEVRVLTQFDAAPHVRIDPGELQQVVINIIINASQAME
GKGTLAISVAGEVRDAQTGVALRIADTGPGIPMERIDHIFDPFFTTKQGEGTGLGLSISQTLIQRAGGRITVRNRRARGA
EFLIWLPEPTDADDPDQR

Sequences:

>Translated_658_residues
MLKSVRLRLLILALLPLIVLMPLLMVVAMARWNADYDKILIEKVESDLRIAEQYLGHLQSNSARALQGLAQSAEIADVLT
RDPGLTPAFLEQSQDRLGLDFLYLLQGDELTDAKEKWPVIFQAAAGRMVSEIDILSASDLAALSPALAAEARIELIETEA
AAPADHTVEDRGMVVHTAAPVRVNGEIRVLVGGILLNRNLDFIDTLNELVYLQGQYSDGRQGTATLFLSDTRVSTNVRLF
EDVRALGTRVSAAVRDAVLGKGRTWLDSAFVVNDWYISGYLPISDSFGKRVGMLYVGFLEAPYETAKRAAYWTVVGAFVL
VIALTAPLFLWLAKGIFAPLEQMTRTMARVERGDLTARNQTSARGDEIGQVANHLNTLLDQVQERDRRLRNWAGELNLRV
EERTRELQVANAKLEETFRQLVMSEKLASIGEITAGVAHEINNPVAVIQGNVDVMRMTLGDAAEPVRTELDLIDNQVMRI
GAIVGKLLQFARPSEFGTFAEHVDVAAVVRDCLVLVDHVISKQEVRVLTQFDAAPHVRIDPGELQQVVINIIINASQAME
GKGTLAISVAGEVRDAQTGVALRIADTGPGIPMERIDHIFDPFFTTKQGEGTGLGLSISQTLIQRAGGRITVRNRRARGA
EFLIWLPEPTDADDPDQR
>Mature_658_residues
MLKSVRLRLLILALLPLIVLMPLLMVVAMARWNADYDKILIEKVESDLRIAEQYLGHLQSNSARALQGLAQSAEIADVLT
RDPGLTPAFLEQSQDRLGLDFLYLLQGDELTDAKEKWPVIFQAAAGRMVSEIDILSASDLAALSPALAAEARIELIETEA
AAPADHTVEDRGMVVHTAAPVRVNGEIRVLVGGILLNRNLDFIDTLNELVYLQGQYSDGRQGTATLFLSDTRVSTNVRLF
EDVRALGTRVSAAVRDAVLGKGRTWLDSAFVVNDWYISGYLPISDSFGKRVGMLYVGFLEAPYETAKRAAYWTVVGAFVL
VIALTAPLFLWLAKGIFAPLEQMTRTMARVERGDLTARNQTSARGDEIGQVANHLNTLLDQVQERDRRLRNWAGELNLRV
EERTRELQVANAKLEETFRQLVMSEKLASIGEITAGVAHEINNPVAVIQGNVDVMRMTLGDAAEPVRTELDLIDNQVMRI
GAIVGKLLQFARPSEFGTFAEHVDVAAVVRDCLVLVDHVISKQEVRVLTQFDAAPHVRIDPGELQQVVINIIINASQAME
GKGTLAISVAGEVRDAQTGVALRIADTGPGIPMERIDHIFDPFFTTKQGEGTGLGLSISQTLIQRAGGRITVRNRRARGA
EFLIWLPEPTDADDPDQR

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=32.4786324786325, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=36.864406779661, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1789149, Length=427, Percent_Identity=23.1850117096019, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790300, Length=238, Percent_Identity=28.9915966386555, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693157, Length=255, Percent_Identity=27.4509803921569, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790346, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=26.7241379310345, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=220, Percent_Identity=25, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=212, Percent_Identity=29.2452830188679, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081816, Length=355, Percent_Identity=24.5070422535211, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72357; Mature: 72357

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSVRLRLLILALLPLIVLMPLLMVVAMARWNADYDKILIEKVESDLRIAEQYLGHLQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NSARALQGLAQSAEIADVLTRDPGLTPAFLEQSQDRLGLDFLYLLQGDELTDAKEKWPVI
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCHHHCCEE
FQAAAGRMVSEIDILSASDLAALSPALAAEARIELIETEAAAPADHTVEDRGMVVHTAAP
EEHHCCCHHHHHHHCCCCCHHHHCHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECCC
VRVNGEIRVLVGGILLNRNLDFIDTLNELVYLQGQYSDGRQGTATLFLSDTRVSTNVRLF
EEECCEEEEEEEEEEECCCCHHHHHHHHHEEEECCCCCCCCCEEEEEEECCCCCCCHHHH
EDVRALGTRVSAAVRDAVLGKGRTWLDSAFVVNDWYISGYLPISDSFGKRVGMLYVGFLE
HHHHHHHHHHHHHHHHHHHCCCCCHHCCEEEEEEEEEEEEEECCHHCCCHHHHHEEEHHH
APYETAKRAAYWTVVGAFVLVIALTAPLFLWLAKGIFAPLEQMTRTMARVERGDLTARNQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TSARGDEIGQVANHLNTLLDQVQERDRRLRNWAGELNLRVEERTRELQVANAKLEETFRQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHH
LVMSEKLASIGEITAGVAHEINNPVAVIQGNVDVMRMTLGDAAEPVRTELDLIDNQVMRI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
GAIVGKLLQFARPSEFGTFAEHVDVAAVVRDCLVLVDHVISKQEVRVLTQFDAAPHVRID
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEC
PGELQQVVINIIINASQAMEGKGTLAISVAGEVRDAQTGVALRIADTGPGIPMERIDHIF
HHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
DPFFTTKQGEGTGLGLSISQTLIQRAGGRITVRNRRARGAEFLIWLPEPTDADDPDQR
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLKSVRLRLLILALLPLIVLMPLLMVVAMARWNADYDKILIEKVESDLRIAEQYLGHLQS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NSARALQGLAQSAEIADVLTRDPGLTPAFLEQSQDRLGLDFLYLLQGDELTDAKEKWPVI
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCHHHCCEE
FQAAAGRMVSEIDILSASDLAALSPALAAEARIELIETEAAAPADHTVEDRGMVVHTAAP
EEHHCCCHHHHHHHCCCCCHHHHCHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECCC
VRVNGEIRVLVGGILLNRNLDFIDTLNELVYLQGQYSDGRQGTATLFLSDTRVSTNVRLF
EEECCEEEEEEEEEEECCCCHHHHHHHHHEEEECCCCCCCCCEEEEEEECCCCCCCHHHH
EDVRALGTRVSAAVRDAVLGKGRTWLDSAFVVNDWYISGYLPISDSFGKRVGMLYVGFLE
HHHHHHHHHHHHHHHHHHHCCCCCHHCCEEEEEEEEEEEEEECCHHCCCHHHHHEEEHHH
APYETAKRAAYWTVVGAFVLVIALTAPLFLWLAKGIFAPLEQMTRTMARVERGDLTARNQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TSARGDEIGQVANHLNTLLDQVQERDRRLRNWAGELNLRVEERTRELQVANAKLEETFRQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHH
LVMSEKLASIGEITAGVAHEINNPVAVIQGNVDVMRMTLGDAAEPVRTELDLIDNQVMRI
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
GAIVGKLLQFARPSEFGTFAEHVDVAAVVRDCLVLVDHVISKQEVRVLTQFDAAPHVRID
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEC
PGELQQVVINIIINASQAMEGKGTLAISVAGEVRDAQTGVALRIADTGPGIPMERIDHIF
HHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
DPFFTTKQGEGTGLGLSISQTLIQRAGGRITVRNRRARGAEFLIWLPEPTDADDPDQR
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]