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The map label for this gene is rbsB [H]

Identifier: 99080209

GI number: 99080209

Start: 377973

End: 378989

Strand: Direct

Name: rbsB [H]

Synonym: TM1040_0368

Alternate gene names: 99080209

Gene position: 377973-378989 (Clockwise)

Preceding gene: 99080205

Following gene: 99080210

Centisome position: 11.81

GC content: 61.06

Gene sequence:

>1017_bases
ATGAAGAAACTACTGCTGACCACCATTGCCGGTGTGGGCTTGCTGGCGGGTGCTGCGCAAGCGGATGAAAAGGTGACGGC
CTGCCTGATCACCAAAACGGATACCAACCCGTTCTTTGTCAAAATGAAAGAAGGGGCGACCGCCAAGGCCGAGGAACTGG
GCATGGAGCTCAAGGCGTTTGCGGGCAAGATCGACGGGGACCATGAAACCCAGGTGGCCGCGATTGAGACCTGCATTGCA
GACGGCGCCAAGGGTATCCTGCTCACCGCGTCTGATACGTCTTCGATTGTACCCGCTGTTCAGCAGGCGCGCGATGCGGG
CCTCGTGGTGATCGCACTCGATACACCTCTCAACCCGATTGATGCGGCTGATGCGACCTTTGCCACCGACAACTTCCTTG
CCGGCGAGCTGATTGGCCAGTGGGCGGCGGCGGCCCTTGGGGATGACGCGGCCAATGCCAAGATTGGCATGCTCGATCTG
GCCGTGAGCCAGCCCACCGTCGGCGTGCTGCGCGATCAGGGCTTCTTGCAGGGCTTTGGCATCGACATCGGGGACCCCAA
CAAATGGGGTGACGAGACCGACCCGCGGATCGTCGGCAATGACGTCACGGCGGGCAATGAGGAAGGTGGCCGCAAGGCGA
TGGAGAATCTTCTCGCCAAAGATCCAATGATCAACGTGGTTTACACCATCAACGAGCCTGCCGCTGCCGGGGCCTATGAG
GCGCTGAAGTCCATCGGTCGGGAAAACGATGTGCTCATCGTCTCCGTCGATGGCGGCTGCCCTGGCGTGCAGAACGTCAA
GGATGGCGTGATCGGCGCCACCTCGCAGCAATACCCGCTGATGATGGCCTCGCTGGGCGTTGAGGCGATTAAGAAATGGG
CCGAAGAAGGCGTGAAGCCGGAGCCTACTCCGGGCAAGGCGTTCTTTGACACCGGCGTTGCGCTGGTGACGGACCAGCCG
GTTGACGGTGTTGAAAGCATCGACACCACAGAGGGCACCAACCTCTGCTGGGGCTGA

Upstream 100 bases:

>100_bases
TCACGGCTTTGATGCAGGCATCGACCGCAACAGCCCCACGGAGGAAGAGGGCTGAGCGACGCAAACTGACTGAGTAGAAT
GTTGTTTCTGGGAGGAGACC

Downstream 100 bases:

>100_bases
TTTGACCCAAAGGCGAGGCCCACGCGGCCTCGCCTGATCCGGAAATGACTGCCTTTACCTCGAGGCGAGGCGTCAAAGGG
CGTTCTCAGCACTCAATTCA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKKLLLTTIAGVGLLAGAAQADEKVTACLITKTDTNPFFVKMKEGATAKAEELGMELKAFAGKIDGDHETQVAAIETCIA
DGAKGILLTASDTSSIVPAVQQARDAGLVVIALDTPLNPIDAADATFATDNFLAGELIGQWAAAALGDDAANAKIGMLDL
AVSQPTVGVLRDQGFLQGFGIDIGDPNKWGDETDPRIVGNDVTAGNEEGGRKAMENLLAKDPMINVVYTINEPAAAGAYE
ALKSIGRENDVLIVSVDGGCPGVQNVKDGVIGATSQQYPLMMASLGVEAIKKWAEEGVKPEPTPGKAFFDTGVALVTDQP
VDGVESIDTTEGTNLCWG

Sequences:

>Translated_338_residues
MKKLLLTTIAGVGLLAGAAQADEKVTACLITKTDTNPFFVKMKEGATAKAEELGMELKAFAGKIDGDHETQVAAIETCIA
DGAKGILLTASDTSSIVPAVQQARDAGLVVIALDTPLNPIDAADATFATDNFLAGELIGQWAAAALGDDAANAKIGMLDL
AVSQPTVGVLRDQGFLQGFGIDIGDPNKWGDETDPRIVGNDVTAGNEEGGRKAMENLLAKDPMINVVYTINEPAAAGAYE
ALKSIGRENDVLIVSVDGGCPGVQNVKDGVIGATSQQYPLMMASLGVEAIKKWAEEGVKPEPTPGKAFFDTGVALVTDQP
VDGVESIDTTEGTNLCWG
>Mature_338_residues
MKKLLLTTIAGVGLLAGAAQADEKVTACLITKTDTNPFFVKMKEGATAKAEELGMELKAFAGKIDGDHETQVAAIETCIA
DGAKGILLTASDTSSIVPAVQQARDAGLVVIALDTPLNPIDAADATFATDNFLAGELIGQWAAAALGDDAANAKIGMLDL
AVSQPTVGVLRDQGFLQGFGIDIGDPNKWGDETDPRIVGNDVTAGNEEGGRKAMENLLAKDPMINVVYTINEPAAAGAYE
ALKSIGRENDVLIVSVDGGCPGVQNVKDGVIGATSQQYPLMMASLGVEAIKKWAEEGVKPEPTPGKAFFDTGVALVTDQP
VDGVESIDTTEGTNLCWG

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=298, Percent_Identity=30.2013422818792, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790526, Length=298, Percent_Identity=24.8322147651007, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790674, Length=267, Percent_Identity=26.2172284644195, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34974; Mature: 34974

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLTTIAGVGLLAGAAQADEKVTACLITKTDTNPFFVKMKEGATAKAEELGMELKAF
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHCCHHHHH
AGKIDGDHETQVAAIETCIADGAKGILLTASDTSSIVPAVQQARDAGLVVIALDTPLNPI
HCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCC
DAADATFATDNFLAGELIGQWAAAALGDDAANAKIGMLDLAVSQPTVGVLRDQGFLQGFG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCCEEECC
IDIGDPNKWGDETDPRIVGNDVTAGNEEGGRKAMENLLAKDPMINVVYTINEPAAAGAYE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
ALKSIGRENDVLIVSVDGGCPGVQNVKDGVIGATSQQYPLMMASLGVEAIKKWAEEGVKP
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCC
EPTPGKAFFDTGVALVTDQPVDGVESIDTTEGTNLCWG
CCCCCCHHHHCCEEEEECCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKLLLTTIAGVGLLAGAAQADEKVTACLITKTDTNPFFVKMKEGATAKAEELGMELKAF
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHCCHHHHH
AGKIDGDHETQVAAIETCIADGAKGILLTASDTSSIVPAVQQARDAGLVVIALDTPLNPI
HCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCC
DAADATFATDNFLAGELIGQWAAAALGDDAANAKIGMLDLAVSQPTVGVLRDQGFLQGFG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCCEEECC
IDIGDPNKWGDETDPRIVGNDVTAGNEEGGRKAMENLLAKDPMINVVYTINEPAAAGAYE
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
ALKSIGRENDVLIVSVDGGCPGVQNVKDGVIGATSQQYPLMMASLGVEAIKKWAEEGVKP
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCC
EPTPGKAFFDTGVALVTDQPVDGVESIDTTEGTNLCWG
CCCCCCHHHHCCEEEEECCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]