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The map label for this gene is pucI [H]

Identifier: 99080179

GI number: 99080179

Start: 342242

End: 343732

Strand: Direct

Name: pucI [H]

Synonym: TM1040_0338

Alternate gene names: 99080179

Gene position: 342242-343732 (Clockwise)

Preceding gene: 99080178

Following gene: 99080180

Centisome position: 10.69

GC content: 60.23

Gene sequence:

>1491_bases
ATGACTGACATAACAAGCGGTTATGCCGCCTCAGAGGGTGCCGCGCACGCGCAGCTGGACCGTGCGCGCCTGGACCCCGA
ACTCTATAACGAGGATCAACTGCCCACCACCGCCGCCGAGCGCACATGGAATTGGCTGTCGATTTCGGCGCTTTGGGTCG
GGATGGTGGTCTGTATCCCAACGTATCTTCTGGCGTCCTATCTGATTGGCGCGGGAATGAGCTGGGATCAGGCGGTTCTG
ACCATTCTGGCGGCCAATGCCATCGTGCTCATCCCGATGGTGCTGGTGGGCCATGCCGGCACCAAATACGGCATCCCGTT
TCCGGTGCTGTTGCGCGCGTCCTTTGGTCCCGTGGGGGCCAAGATCCCGGCTGTTGCCCGTGGGATCGTGGCCTGCGGCT
GGTTTGGCATCCAGACATGGGTTGGCGGTTCTGCGATCTTTGTGATCGTCAACAAGCTCACCGGAGGCGCGCTCGCGGCG
GAGGCGCTGCCGCTTCTGGGGATCAGCCTTGGCGAATTTGTCTGCTTCTTGGCGTTCTGGGGCCTGCATCTCTACTTCAT
CAAGAACGGCACCGAGTCGATCCGCTGGCTTGAGACCTATGCCGCGCCGTTCCTGCTGGCGATGGGGCTTGCGCTCCTGG
CATGGGCCTATTCTGCGGCGGGCGGATTTGGCGAGATGCTCTCCACCCCCAGCGCCTTTGATGTGGGCCAGCCCAAAGAG
GGGCAGTTCTGGGCGGTGTTCTGGCCAAGCCTGACGGGTATGATTGGCTATTGGGCGACGCTTGCGCTCAATATTCCGGA
TTTCACCCGTCACGCCCGCAGCCAGAAAGATCAGTTGGTGGGGCAGATGGTGGGTCTGCCGATCCCGATGGCGTTGTTTG
CCTTTATCGCCTCTGCTGTGACCTCGGCCACGGTTGTGATCTTTGGTGAGGCGATCTGGGATCCGGTGCAGCTCTCTGAA
CGCATGGGCGGCTCTGCGGTGATCATCGCGCTTTTTGCGCTGATCGTGGCGACGCTGACGACAAACCTTGCGGCCAATGT
GGTGGCCCCGGCGCATGGGTTTGCCAATCTTGCGCCAAGCAAGATCAACCTTAAGCGTGGCGGCTATATCACGGCAGCCA
TCGGGATCGCGATGTTCCCGTGGATTCTGGTGAACCACATCGTTGGGTGGCTGATTGCCTATTCCGCGCTTTTGGGGCCG
ATCGCGGGCGTGATGCTGGCGGATTATTACCTCTTGCGCAAAACCCGGCTCGAGGTTGCGGATCTCTTTAAATCAAACGG
CATCTATGCGGGGCACAATGGCACCAACTGGGCCGGTGTCCTGGCGCTGGTCATCGGCATTCTGCCAAATCTGCCGGGCT
TTCTGGCCGGGGTGGGGCTTACGGATGGGACCTCGCCGTTCTTTGCGATGATCTATACCTACGCCTGGTTTGTGGGGCTC
TTTGTTGCGGGCGCGGCCTATTTGCTGCTCTCCAAAATGGTTAACAAATAA

Upstream 100 bases:

>100_bases
TCTTGCGTCTCACATTTTGTCCCCGCACGTCGGGTTTTTTTGGTTTGCGCCCTTTGAAAGAACACGCCACAAATCCGGCT
GTAAGGGAGGAAAACTAGAA

Downstream 100 bases:

>100_bases
CAACTGTTGCTCCGGGGGAAGACCCCGGAGCGCCTTGATTTTAGCGCCAACTATCCAGCGTCTAGCGACAAAAAGCACCC
TCAACTCAAAATGTTGACAT

Product: NCS1 nucleoside transporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTDITSGYAASEGAAHAQLDRARLDPELYNEDQLPTTAAERTWNWLSISALWVGMVVCIPTYLLASYLIGAGMSWDQAVL
TILAANAIVLIPMVLVGHAGTKYGIPFPVLLRASFGPVGAKIPAVARGIVACGWFGIQTWVGGSAIFVIVNKLTGGALAA
EALPLLGISLGEFVCFLAFWGLHLYFIKNGTESIRWLETYAAPFLLAMGLALLAWAYSAAGGFGEMLSTPSAFDVGQPKE
GQFWAVFWPSLTGMIGYWATLALNIPDFTRHARSQKDQLVGQMVGLPIPMALFAFIASAVTSATVVIFGEAIWDPVQLSE
RMGGSAVIIALFALIVATLTTNLAANVVAPAHGFANLAPSKINLKRGGYITAAIGIAMFPWILVNHIVGWLIAYSALLGP
IAGVMLADYYLLRKTRLEVADLFKSNGIYAGHNGTNWAGVLALVIGILPNLPGFLAGVGLTDGTSPFFAMIYTYAWFVGL
FVAGAAYLLLSKMVNK

Sequences:

>Translated_496_residues
MTDITSGYAASEGAAHAQLDRARLDPELYNEDQLPTTAAERTWNWLSISALWVGMVVCIPTYLLASYLIGAGMSWDQAVL
TILAANAIVLIPMVLVGHAGTKYGIPFPVLLRASFGPVGAKIPAVARGIVACGWFGIQTWVGGSAIFVIVNKLTGGALAA
EALPLLGISLGEFVCFLAFWGLHLYFIKNGTESIRWLETYAAPFLLAMGLALLAWAYSAAGGFGEMLSTPSAFDVGQPKE
GQFWAVFWPSLTGMIGYWATLALNIPDFTRHARSQKDQLVGQMVGLPIPMALFAFIASAVTSATVVIFGEAIWDPVQLSE
RMGGSAVIIALFALIVATLTTNLAANVVAPAHGFANLAPSKINLKRGGYITAAIGIAMFPWILVNHIVGWLIAYSALLGP
IAGVMLADYYLLRKTRLEVADLFKSNGIYAGHNGTNWAGVLALVIGILPNLPGFLAGVGLTDGTSPFFAMIYTYAWFVGL
FVAGAAYLLLSKMVNK
>Mature_495_residues
TDITSGYAASEGAAHAQLDRARLDPELYNEDQLPTTAAERTWNWLSISALWVGMVVCIPTYLLASYLIGAGMSWDQAVLT
ILAANAIVLIPMVLVGHAGTKYGIPFPVLLRASFGPVGAKIPAVARGIVACGWFGIQTWVGGSAIFVIVNKLTGGALAAE
ALPLLGISLGEFVCFLAFWGLHLYFIKNGTESIRWLETYAAPFLLAMGLALLAWAYSAAGGFGEMLSTPSAFDVGQPKEG
QFWAVFWPSLTGMIGYWATLALNIPDFTRHARSQKDQLVGQMVGLPIPMALFAFIASAVTSATVVIFGEAIWDPVQLSER
MGGSAVIIALFALIVATLTTNLAANVVAPAHGFANLAPSKINLKRGGYITAAIGIAMFPWILVNHIVGWLIAYSALLGPI
AGVMLADYYLLRKTRLEVADLFKSNGIYAGHNGTNWAGVLALVIGILPNLPGFLAGVGLTDGTSPFFAMIYTYAWFVGLF
VAGAAYLLLSKMVNK

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=483, Percent_Identity=28.9855072463768, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786530, Length=381, Percent_Identity=26.7716535433071, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322219, Length=497, Percent_Identity=28.5714285714286, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=472, Percent_Identity=28.1779661016949, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319429, Length=511, Percent_Identity=28.1800391389432, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323266, Length=456, Percent_Identity=24.780701754386, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324645, Length=445, Percent_Identity=23.8202247191011, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324766, Length=456, Percent_Identity=22.5877192982456, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 52760; Mature: 52628

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDITSGYAASEGAAHAQLDRARLDPELYNEDQLPTTAAERTWNWLSISALWVGMVVCIP
CCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
TYLLASYLIGAGMSWDQAVLTILAANAIVLIPMVLVGHAGTKYGIPFPVLLRASFGPVGA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCC
KIPAVARGIVACGWFGIQTWVGGSAIFVIVNKLTGGALAAEALPLLGISLGEFVCFLAFW
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLHLYFIKNGTESIRWLETYAAPFLLAMGLALLAWAYSAAGGFGEMLSTPSAFDVGQPKE
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCC
GQFWAVFWPSLTGMIGYWATLALNIPDFTRHARSQKDQLVGQMVGLPIPMALFAFIASAV
CCEEEEECCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TSATVVIFGEAIWDPVQLSERMGGSAVIIALFALIVATLTTNLAANVVAPAHGFANLAPS
HHHEEEEEHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
KINLKRGGYITAAIGIAMFPWILVNHIVGWLIAYSALLGPIAGVMLADYYLLRKTRLEVA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLFKSNGIYAGHNGTNWAGVLALVIGILPNLPGFLAGVGLTDGTSPFFAMIYTYAWFVGL
HHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
FVAGAAYLLLSKMVNK
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDITSGYAASEGAAHAQLDRARLDPELYNEDQLPTTAAERTWNWLSISALWVGMVVCIP
CCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
TYLLASYLIGAGMSWDQAVLTILAANAIVLIPMVLVGHAGTKYGIPFPVLLRASFGPVGA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCC
KIPAVARGIVACGWFGIQTWVGGSAIFVIVNKLTGGALAAEALPLLGISLGEFVCFLAFW
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLHLYFIKNGTESIRWLETYAAPFLLAMGLALLAWAYSAAGGFGEMLSTPSAFDVGQPKE
HHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCC
GQFWAVFWPSLTGMIGYWATLALNIPDFTRHARSQKDQLVGQMVGLPIPMALFAFIASAV
CCEEEEECCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TSATVVIFGEAIWDPVQLSERMGGSAVIIALFALIVATLTTNLAANVVAPAHGFANLAPS
HHHEEEEEHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
KINLKRGGYITAAIGIAMFPWILVNHIVGWLIAYSALLGPIAGVMLADYYLLRKTRLEVA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLFKSNGIYAGHNGTNWAGVLALVIGILPNLPGFLAGVGLTDGTSPFFAMIYTYAWFVGL
HHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
FVAGAAYLLLSKMVNK
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]