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The map label for this gene is gcvA [H]

Identifier: 99080172

GI number: 99080172

Start: 335647

End: 336549

Strand: Direct

Name: gcvA [H]

Synonym: TM1040_0331

Alternate gene names: 99080172

Gene position: 335647-336549 (Clockwise)

Preceding gene: 99080170

Following gene: 99080174

Centisome position: 10.49

GC content: 66.22

Gene sequence:

>903_bases
ATGCGGCGCTTGCCTCCGCTCCATGCACTTCGCGCCTTTGAAGCTGCTGCACGGCACCTGCATTTTGCGCGGGCCGCGGC
AGAGCTCCACTTGACGCCAACGGCGATCAGCCACCAGATCCGGCTCCTCGAAGAGATACTCGGTGTCAAACTCTTTCACC
GCTACCCGCGCCCGATCCGGCTGTCGGCAGAGGGCGCGGCGCTTTATCCGGTTCTGCGTGAGGGTTTTGATCGCATGGCC
GATGTGATCGCTGCGCTTTCAGCACCAGATGCGCGCCGCCCGCTGACGGTCTCGGTCACGGTGGCCTTCGCGAGCCTCTG
GCTGATGCGGCGTCTGCCCGCTCTGCGCAACGAGGCGGGGTTGAACCTCAAGGTCGAAGCCGACAATCAACCGGTGGATC
TTTCCGGGGGGGATGTGGATTTTGCGGTGCGATACGCCCGACAGCCCGGCCCGGAGGGGCATTGGCGGGCACTTTTCGAG
GACCGGCTGGTGCCGCTCTGCGCGCCCGACCTGCTCTGTCGGCACCCGGTTGTGCGGGACGCGGATGTGCTGGGGTTGCC
TCTGATTGCCTACCGCTGGAGCGGGGGGGCGGAGGCCGCGCCAAGCTGGCACGGCTGGTCGCGCGCCGCGGGGCTTTCGC
AGGACGTGCCGGAGGTCTCGCAGCATTTTTCCGAGGAAATTCATGCTATTGATTCCGCGATTGCAGGGCAGGGTGTGGTG
CTTGCATCCAGCGTTCTGGCCGCCGCATCCGTTGCCGCCGGGCAATTGAAGCCGCTGTCCACAACCTCATTGCCGGGGCG
CAGCTTTTGGGCGGTTGCGCGCAACGATCACCCGCGTCTCGAGGAGGTGGAGCGTTTTGCCGCCTGGGCGCGCGACTCTG
CAGAGGCGCCGGTTGCCCCTTAG

Upstream 100 bases:

>100_bases
TATCTCAGGCTCGGGATGAAGCAGATTCATCCACCTGTCGGCCAGATTGATCCCAAAGACATCGCCTCAGACAGAACGCC
AGACAGAAAGGATGAGACCG

Downstream 100 bases:

>100_bases
CGGCTGCCTGACCCGATGGGCATCCTGAACGGTCGCGGTCAGATCTCGGTGATCGCCTGTGTGAGCGCCTGCCATTGGTC
GCGCGACACACCCTCTGGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRRLPPLHALRAFEAAARHLHFARAAAELHLTPTAISHQIRLLEEILGVKLFHRYPRPIRLSAEGAALYPVLREGFDRMA
DVIAALSAPDARRPLTVSVTVAFASLWLMRRLPALRNEAGLNLKVEADNQPVDLSGGDVDFAVRYARQPGPEGHWRALFE
DRLVPLCAPDLLCRHPVVRDADVLGLPLIAYRWSGGAEAAPSWHGWSRAAGLSQDVPEVSQHFSEEIHAIDSAIAGQGVV
LASSVLAAASVAAGQLKPLSTTSLPGRSFWAVARNDHPRLEEVERFAAWARDSAEAPVAP

Sequences:

>Translated_300_residues
MRRLPPLHALRAFEAAARHLHFARAAAELHLTPTAISHQIRLLEEILGVKLFHRYPRPIRLSAEGAALYPVLREGFDRMA
DVIAALSAPDARRPLTVSVTVAFASLWLMRRLPALRNEAGLNLKVEADNQPVDLSGGDVDFAVRYARQPGPEGHWRALFE
DRLVPLCAPDLLCRHPVVRDADVLGLPLIAYRWSGGAEAAPSWHGWSRAAGLSQDVPEVSQHFSEEIHAIDSAIAGQGVV
LASSVLAAASVAAGQLKPLSTTSLPGRSFWAVARNDHPRLEEVERFAAWARDSAEAPVAP
>Mature_300_residues
MRRLPPLHALRAFEAAARHLHFARAAAELHLTPTAISHQIRLLEEILGVKLFHRYPRPIRLSAEGAALYPVLREGFDRMA
DVIAALSAPDARRPLTVSVTVAFASLWLMRRLPALRNEAGLNLKVEADNQPVDLSGGDVDFAVRYARQPGPEGHWRALFE
DRLVPLCAPDLLCRHPVVRDADVLGLPLIAYRWSGGAEAAPSWHGWSRAAGLSQDVPEVSQHFSEEIHAIDSAIAGQGVV
LASSVLAAASVAAGQLKPLSTTSLPGRSFWAVARNDHPRLEEVERFAAWARDSAEAPVAP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=33.7931034482759, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI1786448, Length=262, Percent_Identity=35.4961832061069, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788706, Length=310, Percent_Identity=27.741935483871, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=24.1258741258741, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32672; Mature: 32672

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPPLHALRAFEAAARHLHFARAAAELHLTPTAISHQIRLLEEILGVKLFHRYPRPIR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LSAEGAALYPVLREGFDRMADVIAALSAPDARRPLTVSVTVAFASLWLMRRLPALRNEAG
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
LNLKVEADNQPVDLSGGDVDFAVRYARQPGPEGHWRALFEDRLVPLCAPDLLCRHPVVRD
CEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCC
ADVLGLPLIAYRWSGGAEAAPSWHGWSRAAGLSQDVPEVSQHFSEEIHAIDSAIAGQGVV
CCHHCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
LASSVLAAASVAAGQLKPLSTTSLPGRSFWAVARNDHPRLEEVERFAAWARDSAEAPVAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRRLPPLHALRAFEAAARHLHFARAAAELHLTPTAISHQIRLLEEILGVKLFHRYPRPIR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LSAEGAALYPVLREGFDRMADVIAALSAPDARRPLTVSVTVAFASLWLMRRLPALRNEAG
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
LNLKVEADNQPVDLSGGDVDFAVRYARQPGPEGHWRALFEDRLVPLCAPDLLCRHPVVRD
CEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCC
ADVLGLPLIAYRWSGGAEAAPSWHGWSRAAGLSQDVPEVSQHFSEEIHAIDSAIAGQGVV
CCHHCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
LASSVLAAASVAAGQLKPLSTTSLPGRSFWAVARNDHPRLEEVERFAAWARDSAEAPVAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]