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The map label for this gene is mdlY [H]

Identifier: 99080168

GI number: 99080168

Start: 331873

End: 333141

Strand: Direct

Name: mdlY [H]

Synonym: TM1040_0327

Alternate gene names: 99080168

Gene position: 331873-333141 (Clockwise)

Preceding gene: 99080167

Following gene: 99080169

Centisome position: 10.37

GC content: 67.53

Gene sequence:

>1269_bases
ATGAGCGCTGTCCTCGAGGCCACCCAAGAGGCGCTGAGCCGCGCCGAGCAAGCGCCGGAGGTGTTCCTGTCGCGCTTGCC
GGAGCGCGCCCGCGCAGAAGCCCGAGAAAGCGCGGCGCGTCGCTCCAAGCTGGGCCCGCTTGATGGGGCGACAATCTCCT
GGAAGGACATGATTGCCCTAAAGGGTGTCGCGGCGCGTGCAGGCGCGGCCCATGACCCGAAGCTGGCGCCCGCCGTTTCG
GATGCAGACGTCGTGACCCGTGCGCGCAGGGCGGGGCTTGTCAGCATCGGTATCACCAACCAGTCCGAGCTTGCGTTTTC
CGGGATCGGTTACAATCCGCATCACGGCACCCCGCTCCTTGAGGGGCGGGTGCCGGGGGGATCGTCCTCGGGAGCGGCGG
CTTCGGTGGCGCGGGGGATCACCCGGCTCGCGGTGGGCACGGATACCGCTGGCTCTTGCCGGGTGCCAGCGGCGTTTCAG
GGCGTGGTGGGCTTTCGCCCCACACGCGGGCGCTACCCGATGGCGGGGGTGATGCCGCTTGCCCCGAGTTACGACACCGT
AGGCGGCTTTGCCCGCTCGGTGAGTGACATGATCGCGCTGGATGCGGTGCTCTCGGGCGATGAGACCTGTGCGGATGCGG
CCAGCAGCCTTGGGGTGCTCCATGAGGTGCTTGGGGCTGGTGATGTGGCTACAGGGGTCGCGGCGTCTTTCAAAGCGGCG
GTGGTCCGACTTGCTGAGGCGGGGCTGAAGATGGAGCGCGCCAGACAGTCGCCCCTTCTGGACGCATTGGCGCTGATCGC
TGCAGGGGGCTGGCCCGGCGCGCGTGAGGCGGTGGACACCTACGGGCATCTGTTGGCTCCCGAGACGGCTCTGCGCCTTG
ACCCCTTTGTTGCGGAGCGGTTGCGTGTGTCGGCCCGCCTGGACCGCGAACCACTCGAAAAGGTCAAAAGGCAGGCCTGC
AGTCTGCGCGCCAGGATGCAGACCGAAGACACCCTCTATGTGTTGCCCACCGTCGCCATCGAGGCGCCCCTTCTGGCGCC
GCTCTTGGCAGACCCGGAGGCCTTTGCCGCCACCAATGCCGCTGCCCTGCGCCTGACGATGCCTGCCAGCCTGCTCGATT
TGCCAGCGATCTCGCTGCCCATCGGCCAGACGGAGGCGGGCTGCTGGGTGGGCCTGCAGCTGGTCGGGCCGCCACATAGC
GACCGTCGTCTGCTCGCGACATCACAAACGGTCGAAAAGCTGTTGATGAGAAAGGATAAGCTCAGATGA

Upstream 100 bases:

>100_bases
CCAGCATTGGCGCGCAGGTCTATGCCTCCTGGCACCCGAGCGACGGATTCCTTCTCGAGCCGCCGCAGGCCGAGGGGGGC
GATGCGAGGGGCAGGGCGGC

Downstream 100 bases:

>100_bases
TCCGCAATCCGATAAAATGGCCCAATGGCGCCCGCTGCGCCTGTGCGATCACGTTTGATGTCGATGCCGACAGCCTGATC
CATATCGCCCGTCCCGAAGA

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSAVLEATQEALSRAEQAPEVFLSRLPERARAEARESAARRSKLGPLDGATISWKDMIALKGVAARAGAAHDPKLAPAVS
DADVVTRARRAGLVSIGITNQSELAFSGIGYNPHHGTPLLEGRVPGGSSSGAAASVARGITRLAVGTDTAGSCRVPAAFQ
GVVGFRPTRGRYPMAGVMPLAPSYDTVGGFARSVSDMIALDAVLSGDETCADAASSLGVLHEVLGAGDVATGVAASFKAA
VVRLAEAGLKMERARQSPLLDALALIAAGGWPGAREAVDTYGHLLAPETALRLDPFVAERLRVSARLDREPLEKVKRQAC
SLRARMQTEDTLYVLPTVAIEAPLLAPLLADPEAFAATNAAALRLTMPASLLDLPAISLPIGQTEAGCWVGLQLVGPPHS
DRRLLATSQTVEKLLMRKDKLR

Sequences:

>Translated_422_residues
MSAVLEATQEALSRAEQAPEVFLSRLPERARAEARESAARRSKLGPLDGATISWKDMIALKGVAARAGAAHDPKLAPAVS
DADVVTRARRAGLVSIGITNQSELAFSGIGYNPHHGTPLLEGRVPGGSSSGAAASVARGITRLAVGTDTAGSCRVPAAFQ
GVVGFRPTRGRYPMAGVMPLAPSYDTVGGFARSVSDMIALDAVLSGDETCADAASSLGVLHEVLGAGDVATGVAASFKAA
VVRLAEAGLKMERARQSPLLDALALIAAGGWPGAREAVDTYGHLLAPETALRLDPFVAERLRVSARLDREPLEKVKRQAC
SLRARMQTEDTLYVLPTVAIEAPLLAPLLADPEAFAATNAAALRLTMPASLLDLPAISLPIGQTEAGCWVGLQLVGPPHS
DRRLLATSQTVEKLLMRKDKLR
>Mature_421_residues
SAVLEATQEALSRAEQAPEVFLSRLPERARAEARESAARRSKLGPLDGATISWKDMIALKGVAARAGAAHDPKLAPAVSD
ADVVTRARRAGLVSIGITNQSELAFSGIGYNPHHGTPLLEGRVPGGSSSGAAASVARGITRLAVGTDTAGSCRVPAAFQG
VVGFRPTRGRYPMAGVMPLAPSYDTVGGFARSVSDMIALDAVLSGDETCADAASSLGVLHEVLGAGDVATGVAASFKAAV
VRLAEAGLKMERARQSPLLDALALIAAGGWPGAREAVDTYGHLLAPETALRLDPFVAERLRVSARLDREPLEKVKRQACS
LRARMQTEDTLYVLPTVAIEAPLLAPLLADPEAFAATNAAALRLTMPASLLDLPAISLPIGQTEAGCWVGLQLVGPPHSD
RRLLATSQTVEKLLMRKDKLR

Specific function: Hydrolyzes both the R- and the S-enantiomers of mandelamide, and phenylacetamide. Has lower activity on 3- phenylpropionaide and lactamide. Does not hydrolyze benzamide. Hydrolyzes esters and amides with little steric bulk. Preferentially hydrolyzes aroma

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=432, Percent_Identity=26.3888888888889, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=449, Percent_Identity=25.8351893095768, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=134, Percent_Identity=32.8358208955224, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=204, Percent_Identity=29.9019607843137, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=137, Percent_Identity=39.4160583941606, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=239, Percent_Identity=26.7782426778243, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=29.4573643410853, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=197, Percent_Identity=32.994923857868, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=214, Percent_Identity=35.981308411215, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=198, Percent_Identity=32.8282828282828, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=198, Percent_Identity=32.8282828282828, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648437, Length=198, Percent_Identity=32.8282828282828, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648441, Length=180, Percent_Identity=32.7777777777778, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648439, Length=180, Percent_Identity=32.7777777777778, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652985, Length=434, Percent_Identity=23.2718894009217, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922090, Length=434, Percent_Identity=23.2718894009217, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652981, Length=434, Percent_Identity=23.2718894009217, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24652983, Length=434, Percent_Identity=23.2718894009217, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644968, Length=455, Percent_Identity=23.5164835164835, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.86 [H]

Molecular weight: Translated: 44016; Mature: 43885

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVLEATQEALSRAEQAPEVFLSRLPERARAEARESAARRSKLGPLDGATISWKDMIAL
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHH
KGVAARAGAAHDPKLAPAVSDADVVTRARRAGLVSIGITNQSELAFSGIGYNPHHGTPLL
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCEEECCCCCCCCCCCEE
EGRVPGGSSSGAAASVARGITRLAVGTDTAGSCRVPAAFQGVVGFRPTRGRYPMAGVMPL
ECCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
APSYDTVGGFARSVSDMIALDAVLSGDETCADAASSLGVLHEVLGAGDVATGVAASFKAA
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VVRLAEAGLKMERARQSPLLDALALIAAGGWPGAREAVDTYGHLLAPETALRLDPFVAER
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHH
LRVSARLDREPLEKVKRQACSLRARMQTEDTLYVLPTVAIEAPLLAPLLADPEAFAATNA
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCHHHHHHHCCCHHHHHCCC
AALRLTMPASLLDLPAISLPIGQTEAGCWVGLQLVGPPHSDRRLLATSQTVEKLLMRKDK
EEEEEECCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHC
LR
CC
>Mature Secondary Structure 
SAVLEATQEALSRAEQAPEVFLSRLPERARAEARESAARRSKLGPLDGATISWKDMIAL
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHH
KGVAARAGAAHDPKLAPAVSDADVVTRARRAGLVSIGITNQSELAFSGIGYNPHHGTPLL
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCEEECCCCCCCCCCCEE
EGRVPGGSSSGAAASVARGITRLAVGTDTAGSCRVPAAFQGVVGFRPTRGRYPMAGVMPL
ECCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
APSYDTVGGFARSVSDMIALDAVLSGDETCADAASSLGVLHEVLGAGDVATGVAASFKAA
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VVRLAEAGLKMERARQSPLLDALALIAAGGWPGAREAVDTYGHLLAPETALRLDPFVAER
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHH
LRVSARLDREPLEKVKRQACSLRARMQTEDTLYVLPTVAIEAPLLAPLLADPEAFAATNA
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCHHHHHHHCCCHHHHHCCC
AALRLTMPASLLDLPAISLPIGQTEAGCWVGLQLVGPPHSDRRLLATSQTVEKLLMRKDK
EEEEEECCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHC
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12670968 [H]