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The map label for this gene is pth

Identifier: 99080055

GI number: 99080055

Start: 230182

End: 230928

Strand: Reverse

Name: pth

Synonym: TM1040_0214

Alternate gene names: 99080055

Gene position: 230928-230182 (Counterclockwise)

Preceding gene: 99080057

Following gene: 99080053

Centisome position: 7.21

GC content: 61.18

Gene sequence:

>747_bases
ATGAAACTCTTTGTAGGGCTTGGCAATCCGGGCGCAAAATACGCCGGAAACCGACACAATATCGGTTTCATGGCGCTGGA
TCAGATCGCTTCAGATCACGGGTTTTCCCCCTGGAAATCGAAATTCCAGGCCCAAATGAGCGAAGGCACCCTGTCAGGTG
AAAAAGTGATCCTGCTTAAGCCCCAGACCTTTATGAACAACTCCGGCCAATCCGTCGGCGAGGCGCTGCGTTTTTTCAAA
CTCACACCGGCGGATGTTGTGGTCTTTCATGACGAACTTGACCTCGCGCCGAGCAAATGCCGCGTGAAAAACGGCGGCGG
CCATGCAGGTCACAACGGGTTGCGCTCGATCCATGCCCATATTGGTGCCGACTACGGCCGGGTGCGCCTCGGGGTGGGCC
ACCCCGGACACAAGGATGCAGTTGCCGGCTACGTTCTGCGGGACTTCCCCAAAGCGGATCAGGGCTGGCTAGATGATCTG
ATGCGCGGGCTATCGGATGGGGCGGCCGCGCTGGCCACGGGCGACAGCGGGCGGTTCATGAACGCGGTCGCATTGCGGGT
CGCGCCACCGCGCAGCTCTACCGGTGAGGCCTCCAAAGGGCGCAAGAAAGCACAGAAGTCCGAGCCGGGGGTCGCAAAAA
CGCCAGCCAAGGCCGCGACGCCCGAAGCCCCCGCCGCGGGCGACATTCCCGCAGCGCCCGAGGACAGCCGTAGCCCGATG
CAAAAGCTGCTCGACAAGTTCAAATAG

Upstream 100 bases:

>100_bases
GAGCGGGAAAATGCACCTGCATATCCCTATCACCGCTGCTCTGGATTTTCCTCTCAAAAAAGGGCATCACTGCGCCCGAA
ACGACAGCGGGACGCAGCGC

Downstream 100 bases:

>100_bases
AGATCCCGAAGCGCCTCGCTTTGGTATCCGCTTTCACAAGGCAGACCACGCCCATTCCGGCCCGGACGCCAAGGCGAAGA
GATCTCATCCCAAGGTCGCG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKLFVGLGNPGAKYAGNRHNIGFMALDQIASDHGFSPWKSKFQAQMSEGTLSGEKVILLKPQTFMNNSGQSVGEALRFFK
LTPADVVVFHDELDLAPSKCRVKNGGGHAGHNGLRSIHAHIGADYGRVRLGVGHPGHKDAVAGYVLRDFPKADQGWLDDL
MRGLSDGAAALATGDSGRFMNAVALRVAPPRSSTGEASKGRKKAQKSEPGVAKTPAKAATPEAPAAGDIPAAPEDSRSPM
QKLLDKFK

Sequences:

>Translated_248_residues
MKLFVGLGNPGAKYAGNRHNIGFMALDQIASDHGFSPWKSKFQAQMSEGTLSGEKVILLKPQTFMNNSGQSVGEALRFFK
LTPADVVVFHDELDLAPSKCRVKNGGGHAGHNGLRSIHAHIGADYGRVRLGVGHPGHKDAVAGYVLRDFPKADQGWLDDL
MRGLSDGAAALATGDSGRFMNAVALRVAPPRSSTGEASKGRKKAQKSEPGVAKTPAKAATPEAPAAGDIPAAPEDSRSPM
QKLLDKFK
>Mature_248_residues
MKLFVGLGNPGAKYAGNRHNIGFMALDQIASDHGFSPWKSKFQAQMSEGTLSGEKVILLKPQTFMNNSGQSVGEALRFFK
LTPADVVVFHDELDLAPSKCRVKNGGGHAGHNGLRSIHAHIGADYGRVRLGVGHPGHKDAVAGYVLRDFPKADQGWLDDL
MRGLSDGAAALATGDSGRFMNAVALRVAPPRSSTGEASKGRKKAQKSEPGVAKTPAKAATPEAPAAGDIPAAPEDSRSPM
QKLLDKFK

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=163, Percent_Identity=36.1963190184049, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787455, Length=163, Percent_Identity=45.398773006135, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_SILST (Q1GK69)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612209.1
- ProteinModelPortal:   Q1GK69
- SMR:   Q1GK69
- STRING:   Q1GK69
- GeneID:   4078662
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0214
- NMPDR:   fig|292414.1.peg.2590
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   TAQSSEM
- PhylomeDB:   Q1GK69
- ProtClustDB:   PRK05426
- BioCyc:   SSP292414:TM1040_0214-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 26219; Mature: 26219

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFVGLGNPGAKYAGNRHNIGFMALDQIASDHGFSPWKSKFQAQMSEGTLSGEKVILLK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEC
PQTFMNNSGQSVGEALRFFKLTPADVVVFHDELDLAPSKCRVKNGGGHAGHNGLRSIHAH
CHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHH
IGADYGRVRLGVGHPGHKDAVAGYVLRDFPKADQGWLDDLMRGLSDGAAALATGDSGRFM
HCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEE
NAVALRVAPPRSSTGEASKGRKKAQKSEPGVAKTPAKAATPEAPAAGDIPAAPEDSRSPM
EEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
QKLLDKFK
HHHHHHCC
>Mature Secondary Structure
MKLFVGLGNPGAKYAGNRHNIGFMALDQIASDHGFSPWKSKFQAQMSEGTLSGEKVILLK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEC
PQTFMNNSGQSVGEALRFFKLTPADVVVFHDELDLAPSKCRVKNGGGHAGHNGLRSIHAH
CHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHHHH
IGADYGRVRLGVGHPGHKDAVAGYVLRDFPKADQGWLDDLMRGLSDGAAALATGDSGRFM
HCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEE
NAVALRVAPPRSSTGEASKGRKKAQKSEPGVAKTPAKAATPEAPAAGDIPAAPEDSRSPM
EEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
QKLLDKFK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA