Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 99079868

GI number: 99079868

Start: 26757

End: 27371

Strand: Reverse

Name: polA [H]

Synonym: TM1040_0027

Alternate gene names: 99079868

Gene position: 27371-26757 (Counterclockwise)

Preceding gene: 99079873

Following gene: 99079867

Centisome position: 0.86

GC content: 58.7

Gene sequence:

>615_bases
GTGGCCACGCATCTTTACCAACACGATTTGCCAAATTCCCTCGATCTCGGACCTGTGGTGGCAATTGACTGCGAAACCAT
GGGGCTCAACCCTCATCGCGATCGTCTCTGCGTTGTGCAGATGTCGAGCGGTGACGGCGACGCGCATCTTGTGCAGATCG
CAAAGGGGCAAACCGACGCTCCGAACCTGACCGCGATGCTTACCAACCCGGATGTGCTCAAGCTGTTTCACTACGGGCGC
TTTGACATCGCGGCACTATACCATCGCTTTGGAGCGCTGGCGGCGCCGGTCTATTGCACCAAGATCGCCAGCCGTCTGGT
GCGCACCTATACGGATCGTCACGGGTTGAAAAACCTGACCCAAGACCTGCTCGGCCAAGACATCTCCAAACAGCAGCAAA
TGAGCGACTGGGGCGCGGAAACACTCACGCGGGCGCAAATGGATTATGCCGCCTCGGACGTTCTACATCTGCACCGATTG
CGCGACGCCCTTGATCAACGCCTTGTGCGCGAGGGGCGTAGCGACCTTGCCCAGGCCTGTTTTGACTTTCTACCGACACG
GGCGCGACTGGATTTGGCCGGCTGGCCAGACACCGATATTTTCGCCCATTCCTGA

Upstream 100 bases:

>100_bases
TGGTCCAGCTTGATCCGCCGCCCTCTGCCTGTTGAGTTCGCCCCTCAAAGAGCCTATGTCGGCATCAACCCATTTTCATC
GCACGATCAGGGACCGCGCT

Downstream 100 bases:

>100_bases
CATCACCCAGCCGTGACTTTGCCCTGAAGACACGCAATGACATAGAAGATACAAGAGAAAGGAGACACCGCAATGGATCG
CTATTCCCGCGTCGTCTCTT

Product: 3'-5' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MATHLYQHDLPNSLDLGPVVAIDCETMGLNPHRDRLCVVQMSSGDGDAHLVQIAKGQTDAPNLTAMLTNPDVLKLFHYGR
FDIAALYHRFGALAAPVYCTKIASRLVRTYTDRHGLKNLTQDLLGQDISKQQQMSDWGAETLTRAQMDYAASDVLHLHRL
RDALDQRLVREGRSDLAQACFDFLPTRARLDLAGWPDTDIFAHS

Sequences:

>Translated_204_residues
MATHLYQHDLPNSLDLGPVVAIDCETMGLNPHRDRLCVVQMSSGDGDAHLVQIAKGQTDAPNLTAMLTNPDVLKLFHYGR
FDIAALYHRFGALAAPVYCTKIASRLVRTYTDRHGLKNLTQDLLGQDISKQQQMSDWGAETLTRAQMDYAASDVLHLHRL
RDALDQRLVREGRSDLAQACFDFLPTRARLDLAGWPDTDIFAHS
>Mature_203_residues
ATHLYQHDLPNSLDLGPVVAIDCETMGLNPHRDRLCVVQMSSGDGDAHLVQIAKGQTDAPNLTAMLTNPDVLKLFHYGRF
DIAALYHRFGALAAPVYCTKIASRLVRTYTDRHGLKNLTQDLLGQDISKQQQMSDWGAETLTRAQMDYAASDVLHLHRLR
DALDQRLVREGRSDLAQACFDFLPTRARLDLAGWPDTDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=141, Percent_Identity=29.7872340425532, Blast_Score=65, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324574, Length=165, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 22797; Mature: 22666

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHLYQHDLPNSLDLGPVVAIDCETMGLNPHRDRLCVVQMSSGDGDAHLVQIAKGQTDA
CCCCCCCCCCCCCCCCCCEEEEECEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCC
PNLTAMLTNPDVLKLFHYGRFDIAALYHRFGALAAPVYCTKIASRLVRTYTDRHGLKNLT
CCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDLLGQDISKQQQMSDWGAETLTRAQMDYAASDVLHLHRLRDALDQRLVREGRSDLAQAC
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDFLPTRARLDLAGWPDTDIFAHS
HHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ATHLYQHDLPNSLDLGPVVAIDCETMGLNPHRDRLCVVQMSSGDGDAHLVQIAKGQTDA
CCCCCCCCCCCCCCCCCEEEEECEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCC
PNLTAMLTNPDVLKLFHYGRFDIAALYHRFGALAAPVYCTKIASRLVRTYTDRHGLKNLT
CCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDLLGQDISKQQQMSDWGAETLTRAQMDYAASDVLHLHRLRDALDQRLVREGRSDLAQAC
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDFLPTRARLDLAGWPDTDIFAHS
HHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]