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The map label for this gene is amiC [H]

Identifier: 94994193

GI number: 94994193

Start: 770187

End: 771641

Strand: Direct

Name: amiC [H]

Synonym: MGAS10750_Spy0797

Alternate gene names: 94994193

Gene position: 770187-771641 (Clockwise)

Preceding gene: 94994192

Following gene: 94994194

Centisome position: 39.76

GC content: 42.34

Gene sequence:

>1455_bases
ATGACTTATCAAGATGCGACGGCTATGGCTATTGCTGTTCAGACTGGTCAGACCACACCACTAGAATTAGTAACCCAGGC
TATTTATAAGGCTAAAAAGTTGAACCCTACCTTAAATGCTATTACCTCAGAACGCTTTGAAGCAGCCCTAGAAGAAGCCA
AACAAAGGGATTTTTCAGGTCTGCCTTTTGCAGGAGTTCCCATTTTTTTGAAAGATTTGGGACAAGAATTAAAAGGTCAC
TCATCAACCTCAGGTTCCAGATTGTTTAAAGAGTATCAAGCGACTAAAACGGATTTATTTGTGAAAAGATTAGAAGCTTT
AGGCTTTATTATTTTAGGGCGCTCCAACACGCCTGAATTTGGCTTTAAAAATATTAGCGATAGCAGTTTGCACGGACCAG
TGAATTTACCAAAGGATAATACACGAAATGCTGGTGGCTCGTCAGGTGGTGCGGCAGCTCTAGTGTCATCAGGAATTAGT
GCACTAGCACCAGCTAGTGATGGTGGTGGTTCTATTCGAATTCCAGCCTCGTTTAATGGCTTAATTGGGCTCAAACCTAG
CCGTGGGCGCATGCCTGTCGGACCTGGTTCTTATCGAAGCTGGCAAGGAGCCTCAGTTCATTTTGCCTTGACCAAATCCG
TTAGAGATACTAGAAATCTACTCTACTATTTACAGATGGAACAAATGGAAAGCCCCTTCCCCCTAGCCACCTTAACAAAA
GATAGTATTTATCAATCGCTACAACGTCCCTTGACAATTGCTTTTTATCAACGGTTAAGTGACGGTAGCCCAGTATCTTT
AGATACTGCAAAAGCTTTAAAACAAGCAGTAACTTGGCTAAGAGAACAAGGGCATCAGCTAGTTGAATTGGAAGAGTTTC
CTGTAAATATGACAGAAGTGATTCGTCATTATTATATTATGAATAGTGTGGAAACGGCTGCTATGTTTGCTGATATTGAA
GACACTTTAGGACGTCCGATGACCAAAGACGATATGGAAACCATGACCTGGGCTATTTACCAATCAGGTAAAGACATCCC
AGCTTGGCGTTACTCACAGGTCCTTCAAAAATGGGATACGTACAGTGCAACCATGGCTAGTTTTCATGAGACTTATGACC
TGTTGTTGACGTTCACAACCAACACACCAGCTCCTAAGCACGGAGAGCTTGTCCCAGATTCGAAACTCATGGCTAACTTG
GCTCAAGCTGAAATTTTCAGCAGTGAAGAACAGTTCAATTTGGTCGAAACCATGTTTGGTAAGAGTCTGGCTATTAATCC
TTACACAGCTCTGCCTAATCTAACTGGCCAACCAGCTATTAGTTTACCTACTTACGAAACTAAGGAAGGTCTCCCAATGG
GCATTCAACTAATTGCAGCCAAAGGTAGAGAGGACCTATTATTAGGAATTGCAGAGCAGTTTGAGGCAGCAGGATTATTG
AAGATTCCTCAATAA

Upstream 100 bases:

>100_bases
CACATTCTAATTTCTAACCTTTTAATGGTGGTTTTCTTTTATTTGTGTTTTAATAAAAAAGAATGTTTTAGGATTCATAT
CTTAGAAAGGAAGAGTAGCT

Downstream 100 bases:

>100_bases
TAATCTTATAAAACAGTTTAACTATTATAGATTAACTTTTTAAAGAGATTGAGCGCTCACTGATAACACAACTTAATCAA
AATGTTATACTATTCTTATC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGLPFAGVPIFLKDLGQELKGH
SSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
ALAPASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIE
DTLGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANL
AQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
KIPQ

Sequences:

>Translated_484_residues
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGLPFAGVPIFLKDLGQELKGH
SSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
ALAPASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIE
DTLGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANL
AQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
KIPQ
>Mature_483_residues
TYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSGLPFAGVPIFLKDLGQELKGHS
STSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGISA
LAPASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTKD
SIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQAVTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIED
TLGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLA
QAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLLK
IPQ

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=510, Percent_Identity=27.0588235294118, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI222831590, Length=525, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=358, Percent_Identity=29.0502793296089, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=532, Percent_Identity=25.187969924812, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=185, Percent_Identity=37.8378378378378, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=522, Percent_Identity=25.095785440613, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=533, Percent_Identity=24.953095684803, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=318, Percent_Identity=29.2452830188679, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=318, Percent_Identity=29.2452830188679, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=154, Percent_Identity=37.6623376623377, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=469, Percent_Identity=24.9466950959488, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=24.5283018867925, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=202, Percent_Identity=29.2079207920792, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24652985, Length=524, Percent_Identity=26.3358778625954, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI19922090, Length=524, Percent_Identity=26.3358778625954, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24652981, Length=524, Percent_Identity=26.3358778625954, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24652983, Length=524, Percent_Identity=26.3358778625954, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24644968, Length=526, Percent_Identity=25.8555133079848, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45550774, Length=196, Percent_Identity=37.7551020408163, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648435, Length=196, Percent_Identity=37.7551020408163, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648437, Length=196, Percent_Identity=37.7551020408163, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=26.6019417475728, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=29.3617021276596, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648441, Length=127, Percent_Identity=41.7322834645669, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648439, Length=127, Percent_Identity=41.7322834645669, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648113, Length=186, Percent_Identity=35.4838709677419, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53031; Mature: 52900

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSG
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
LPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEF
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
GFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGISALAPASDGGGSIRIPASFNG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCCEEEECCCCCC
LIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
EEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHEEEEEHHHCCCCCCCCCCCH
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQAVTWLREQGHQLVELEEFPVNMTEV
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHH
IRHYYIMNSVETAAMFADIEDTLGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLAQAEIFSSEEQFNLVETMFG
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHC
KSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
CCEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCE
KIPQ
ECCC
>Mature Secondary Structure 
TYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSERFEAALEEAKQRDFSG
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
LPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVKRLEALGFIILGRSNTPEF
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
GFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGISALAPASDGGGSIRIPASFNG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCCEEEECCCCCC
LIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTRNLLYYLQMEQMESPFPLATLTK
EEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHEEEEEHHHCCCCCCCCCCCH
DSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQAVTWLREQGHQLVELEEFPVNMTEV
HHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHH
IRHYYIMNSVETAAMFADIEDTLGRPMTKDDMETMTWAIYQSGKDIPAWRYSQVLQKWDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YSATMASFHETYDLLLTFTTNTPAPKHGELVPDSKLMANLAQAEIFSSEEQFNLVETMFG
HHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHC
KSLAINPYTALPNLTGQPAISLPTYETKEGLPMGIQLIAAKGREDLLLGIAEQFEAAGLL
CCEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCE
KIPQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]