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The map label for this gene is pyrB [H]

Identifier: 94994126

GI number: 94994126

Start: 704509

End: 705498

Strand: Direct

Name: pyrB [H]

Synonym: MGAS10750_Spy0730

Alternate gene names: 94994126

Gene position: 704509-705498 (Clockwise)

Preceding gene: 94994125

Following gene: 94994127

Centisome position: 36.37

GC content: 39.19

Gene sequence:

>990_bases
TTGTTGTTAGACTACTTACATAGTCATCAGTCGTTAGTGATAAAGGGGATAATCATGTCAGTTGTAAATAATCGCGTTGC
ACTTACCAATTTAGTTTCAATGGAAGCATTAACCACCGAAGAAGTTTTAGGTCTTATCAACCGTGGTTCAGAATATAAGG
CTGGTAAGGTTGTTATTTCGGATCATCAAAAGGATCTTGTTGCCAATTTATTCTTTGAAAACTCCACCCGAACCCATAAG
TCTTTTGAAGTGGCTGAGAAGAAACTCGGCTTGACAGTTCTTGATTTCAATGCTGATGCAAGTGCGGTTAACAAGGGAGA
AAGTTTGTATGATACTGTGTTAACCATGAGTGCACTTGGAACAGACATCTGTGTGATTCGTCACCCAGAAGATGATTATT
ACAAGGAATTAGTAGAAAGTCCAACTATTACAGCTTCCATTGTCAATGGAGGAGATGGTTCTGGACAACACCCGAGTCAA
TGTTTACTTGACTTATTAACCATCTATGAAGAATTTGGACGCTTTGAAGGCTTAAAGATTGCGATCGCTGGTGACTTAAC
TCACTCTAGGGTGGCAAAGTCCAACATGCAAATCCTCAAAAGATTAGGAGCAGAGCTTTATTTCTATGGACCTGAAGAAT
GGTATTCAGAAGCCTTTAATGCTTACGGCACTTATATTGCTATTGACCAGATTATTAAGGAATTGGATGTTCTTATGTTG
CTGCGTGTACAGCACGAACGTCATGACGGCCATCAGAGTTTTTCAAAAGAAAGTTACCATCAAGCGTTTGGATTGACACA
GGAACGTTACCAACAATTAAAAGATTCTGCTATTATTATGCATCCAGCTCCAGTTAATCGGGATGTGGAAATTGCAGATA
GTTTGGTGGAAGCTCCTAAAGCGAGAATTGTATCTCAGATGGCTAATGGTGTCTTTGTTCGCATGGCTATTATTGAAGCC
ATTTTGAATGGTAGAAATAAAAACTCATGA

Upstream 100 bases:

>100_bases
ATTGATGCTTCAAGTAAATGGTCTTCAAATTTCAGGTGTCGCTTTCTCAACACTTCTTGGCATCATCTTATATCAGGTAC
TTCCTGAAAAATAGGAAGAA

Downstream 100 bases:

>100_bases
TGAAAGATTAAAGCATGTTTAGAAGGTAGGAAAAGGTAGGAAAATGACAAAACGATTACTTATTTTAGAAGACGGCACTA
TTTTTGAAGGAGAACCCTTT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLLDYLHSHQSLVIKGIIMSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRTHK
SFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQ
CLLDLLTIYEEFGRFEGLKIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLML
LRVQHERHDGHQSFSKESYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAIIEA
ILNGRNKNS

Sequences:

>Translated_329_residues
MLLDYLHSHQSLVIKGIIMSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRTHK
SFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQ
CLLDLLTIYEEFGRFEGLKIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLML
LRVQHERHDGHQSFSKESYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAIIEA
ILNGRNKNS
>Mature_329_residues
MLLDYLHSHQSLVIKGIIMSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRTHK
SFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQ
CLLDLLTIYEEFGRFEGLKIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLML
LRVQHERHDGHQSFSKESYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAIIEA
ILNGRNKNS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=267, Percent_Identity=35.2059925093633, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI2367364, Length=270, Percent_Identity=34.4444444444444, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=287, Percent_Identity=33.4494773519164, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=268, Percent_Identity=32.089552238806, Blast_Score=125, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24642586, Length=271, Percent_Identity=33.210332103321, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 36708; Mature: 36708

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDYLHSHQSLVIKGIIMSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVIS
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
DHQKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHCCHHHHHHHHHHHHCC
TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGLKI
CCEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
AIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLML
EEECCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LRVQHERHDGHQSFSKESYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPK
HHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHH
ARIVSQMANGVFVRMAIIEAILNGRNKNS
HHHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLLDYLHSHQSLVIKGIIMSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVIS
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
DHQKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHCCHHHHHHHHHHHHCC
TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGLKI
CCEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
AIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLML
EEECCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LRVQHERHDGHQSFSKESYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPK
HHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHH
ARIVSQMANGVFVRMAIIEAILNGRNKNS
HHHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]