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The map label for this gene is nifS1 [C]

Identifier: 94994114

GI number: 94994114

Start: 693785

End: 694906

Strand: Direct

Name: nifS1 [C]

Synonym: MGAS10750_Spy0718

Alternate gene names: 94994114

Gene position: 693785-694906 (Clockwise)

Preceding gene: 94994111

Following gene: 94994115

Centisome position: 35.82

GC content: 41.98

Gene sequence:

>1122_bases
ATGATTTATTTTGATAATGCAGCCACAACCATACCTTATGGAGAGGCTCTTAAAACTTACCAAGAAGTAGCAACAAAAAT
ATATGGCAATCCCTCAAGTCTGCACCAACTAGGGACAAATGCGTCTCGCATTTTAGAAGCTTCCCGTAAACAGATTGCTG
GTTTGTTGGGTGTTAAGTCAGAAGAAATCTTTTTTACGTCTGGTGGGACTGAAAGTGCTAATTGGGCTATTAAGGGAATT
GCTTTTGAAAAAACCGCTTTTGGCAAACATATTATTATCTCAGCCATTGAACACCCTGCCGTGAGTGAAAGTGTCAAATG
GCTTCTCACACAAGGATTCGAAGTTTCTTATGCTCCAGTGACAACACAGGGAGTTGTTGATGTGAATGCTTTAGCTGAGC
TGATTAGACCAGACACCATCTTAATCTCGATTATGGCTGTTAATAATGAAATGGGAGCTATCCAACCGATTAGAGCCATT
TCAAACCTATTGGCTAACCAACCAACGATCACCTTTCACGTTGATGCTGTTCAAGCCATTGGTAAAATACCACTCTGTGA
TTATATGACTAATCGTGTGGATTTGGCTTCTTTTTCAGGGCACAAATTTCATAGTGTTCGTGGTGTTGGCTTCCTTTATA
AAAAAGCAGGCAAGCGACTCAATCCTCTCTTGTCAGGCGGCGGCCAAGAGCAGGAGTTACGTTCAACCACTGAAAATGTT
GCAGGAATTGCTAGCATGGCCAAAGCATTAAGAATAGTCACTGAAAAACAAGTGAGCGTCTTACCTAAACTAACAGCGAT
GCGAGATGTGATTTACAAAGCTTTGTCCGCTTACCCAGATGTGACAGTATTTTCAGCTCAAGAAGGCTTTGCGCCCAATA
TTTTGACCTTTGGGATTAGAGGGGTTCGAGGAGAAGTTATTGTCCACGCCTTTGAAAAATATGAGATTTATATTTCGACC
ACAAGTGCATGTTCGTCCAAAGCAGGTAAGCCAGCAGGAAGCCTAGTGGCTATGGGGGTTCCTGTAAAAACTGCCCAGAC
AGCCGTTCGTATTAGTCTAGACGATGACAACGATATGGGACAAGTAGAGCAGTTTTTGACCAATATTCAAACAGATTTAT
GA

Upstream 100 bases:

>100_bases
ACAAGGAAGTCCTTTGGGCTTATTGTAATACGAATATTCTTGATTTCATTTTAGCTATTTTGTGATAGAATAAAGTATTG
AGTAAAAATGAAGGTGACAC

Downstream 100 bases:

>100_bases
TAAAACACAGAAAGTTAGGTAAGATTAGGTAAGATGGACTATTCAGAAATTATGGTTCGCCATGGTGAACTATCAACTAA
AGGGAAGAATCGCATGCGAT

Product: cysteine desulfurase / Selenocysteine lyase

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGI
AFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYIST
TSACSSKAGKPAGSLVAMGVPVKTAQTAVRISLDDDNDMGQVEQFLTNIQTDL

Sequences:

>Translated_373_residues
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGI
AFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYIST
TSACSSKAGKPAGSLVAMGVPVKTAQTAVRISLDDDNDMGQVEQFLTNIQTDL
>Mature_373_residues
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGI
AFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYIST
TSACSSKAGKPAGSLVAMGVPVKTAQTAVRISLDDDNDMGQVEQFLTNIQTDL

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=34.2696629213483, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI156713448, Length=406, Percent_Identity=30.7881773399015, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI48994898, Length=357, Percent_Identity=38.0952380952381, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789175, Length=350, Percent_Identity=26.8571428571429, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787970, Length=395, Percent_Identity=24.3037974683544, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=357, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=30.4878048780488, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=357, Percent_Identity=35.8543417366947, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI20129463, Length=360, Percent_Identity=35.5555555555556, Blast_Score=192, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 40203; Mature: 40203

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKS
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
EEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPV
CEEEEECCCCCCCCEEEEEEEEEHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCC
TTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAISNLLANQPTITFHVDAVQAI
CCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHH
GKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
CCCCCHHHHCCCEEEECCCCCCEEEECCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHH
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFAPNILTFGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCC
GVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGSLVAMGVPVKTAQTAVRISLDDDNDMG
CCCCCEEEEEEEEEEEEEEECCHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHH
QVEQFLTNIQTDL
HHHHHHHHHHCCC
>Mature Secondary Structure
MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKS
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
EEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPV
CEEEEECCCCCCCCEEEEEEEEEHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCC
TTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAISNLLANQPTITFHVDAVQAI
CCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHH
GKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRSTTENV
CCCCCHHHHCCCEEEECCCCCCEEEECCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHH
AGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALSAYPDVTVFSAQEGFAPNILTFGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCC
GVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGSLVAMGVPVKTAQTAVRISLDDDNDMG
CCCCCEEEEEEEEEEEEEEECCHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHH
QVEQFLTNIQTDL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]