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The map label for this gene is 94986115

Identifier: 94986115

GI number: 94986115

Start: 2126176

End: 2127429

Strand: Reverse

Name: 94986115

Synonym: Dgeo_2016

Alternate gene names: NA

Gene position: 2127429-2126176 (Counterclockwise)

Preceding gene: 94986116

Following gene: 94986114

Centisome position: 86.23

GC content: 58.13

Gene sequence:

>1254_bases
ATGGCGGGTGAGTGGATAGATACAACGGTTGGCGAAATTGCCCCGTTTTCATATGGCAAGGGTTTGCCGGAACGTGAACG
AAAGCAGACCGGATCGGTGCCTGTTTATGGCTCCAATGGCATTGTTGGTTTTCATGATAGTGCATTGACAGGTGGCCCAA
CGATAGTCATCGGACGCAAAGGAACTGTGGGCGCGGTGCATTATTCGCCTATACCGTGTTGGCCGATTGACACGACCTTC
TTTGTTTCCGATAGCGACCGTTCGCTTGTCAGGTACAGCTACTACTTGCTCAAGTCTCTTGGGCTTGAAAATATGAATGC
TGATAGTGCAGTTCCCGGCCTAAACAGAGACGCTGCACATGCACGGATAGTGCTAATCCCACGAGACAAAGCCGAACAAC
GCGCCATCGCCCATATCCTCGGCACGCTGGACGACAAGATTGAGCTGAACCGCAAGCAGAGCGAGACGCTGGAAGCCATG
GCCCGTGCCTTGTTCAAGGCGTGGTTCGTGGACTTCGAGCCGGTGCGCGCCAAGATGGAGGGCCGCTGGCAGCGCGGCCA
ATCGCTGCCCGGCCTGCCCGCCCACCTCTACGACCTCTTCCCCGACCGGCTGGTGGACTCGGAGTTGGGGGAGATTCCGG
AGGGGTGGCGCGTCTTCGCATTCGGTGATGTAGCGCAACAGGGCAAAGGCGTCGTCAATCCGGGAAACTCGCCGCAGGAC
CTCTTTACCCATTACAGCCTGCCGGCCTTCGATTCTGCGCATTGCCCATCGATAGAACCCGGACATGCCATCAAGAGCAA
CAAGACACCGGTGCCGGATGGCGCCGTGCTGGTCTCAAAACTCAACCCCCATATTCCACGCGTGTGGCACGTCGGGACCG
CCGGCCCTAACGCAGTGTGTTCCACCGAGTTCATCGTGTGGGCGCCCAAGGCACCTGCCAACAGCGCATTCCTGTACTGC
CTCGCGTCATCGCCAGAGTTCAGTGGGGCGATGCATCAACTGGTCACCGGAACATCGAATAGTCATCAGCGCGTCAAACC
CGACCAGCTGCGCGAAATCCGTGTTTTCGCCGCCACCGAGAATGCAATAGAAGCGTTCTCCGAGTGGGTGCGCTCACCAC
TGGAAAAGATCCTGCAAAATCGCCAGCAATCCCGCACTCTCGCCCAACTGCGCGACGCGTTGCTCCCAAGGCTGATCTCC
GGCGAGCTGCGCATTGCCGACGCCGAGAAGCCCATGGAGAAATTTTCGCAATGA

Upstream 100 bases:

>100_bases
GAGGAAAAAATGGCGCGGTTGGCGGCCCAGTGGCGCGAGCAGCGGGCAGCGGCGGCCAAGCTGGATGCGGCGATTGAAGC
CAACCTGAAGGAGCTTGGGT

Downstream 100 bases:

>100_bases
GCGACATGAAGACTTGTTTCAAGCGGGTCGATTACGCCCTTTCTGATCTACTGCACTATATCGATATCGGCGATATCGGC
TTGCCCGATATTCAGCGGCC

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.HindVIIP; Type I restriction enzyme HindVIIP specificity protein; S protein [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MAGEWIDTTVGEIAPFSYGKGLPERERKQTGSVPVYGSNGIVGFHDSALTGGPTIVIGRKGTVGAVHYSPIPCWPIDTTF
FVSDSDRSLVRYSYYLLKSLGLENMNADSAVPGLNRDAAHARIVLIPRDKAEQRAIAHILGTLDDKIELNRKQSETLEAM
ARALFKAWFVDFEPVRAKMEGRWQRGQSLPGLPAHLYDLFPDRLVDSELGEIPEGWRVFAFGDVAQQGKGVVNPGNSPQD
LFTHYSLPAFDSAHCPSIEPGHAIKSNKTPVPDGAVLVSKLNPHIPRVWHVGTAGPNAVCSTEFIVWAPKAPANSAFLYC
LASSPEFSGAMHQLVTGTSNSHQRVKPDQLREIRVFAATENAIEAFSEWVRSPLEKILQNRQQSRTLAQLRDALLPRLIS
GELRIADAEKPMEKFSQ

Sequences:

>Translated_417_residues
MAGEWIDTTVGEIAPFSYGKGLPERERKQTGSVPVYGSNGIVGFHDSALTGGPTIVIGRKGTVGAVHYSPIPCWPIDTTF
FVSDSDRSLVRYSYYLLKSLGLENMNADSAVPGLNRDAAHARIVLIPRDKAEQRAIAHILGTLDDKIELNRKQSETLEAM
ARALFKAWFVDFEPVRAKMEGRWQRGQSLPGLPAHLYDLFPDRLVDSELGEIPEGWRVFAFGDVAQQGKGVVNPGNSPQD
LFTHYSLPAFDSAHCPSIEPGHAIKSNKTPVPDGAVLVSKLNPHIPRVWHVGTAGPNAVCSTEFIVWAPKAPANSAFLYC
LASSPEFSGAMHQLVTGTSNSHQRVKPDQLREIRVFAATENAIEAFSEWVRSPLEKILQNRQQSRTLAQLRDALLPRLIS
GELRIADAEKPMEKFSQ
>Mature_416_residues
AGEWIDTTVGEIAPFSYGKGLPERERKQTGSVPVYGSNGIVGFHDSALTGGPTIVIGRKGTVGAVHYSPIPCWPIDTTFF
VSDSDRSLVRYSYYLLKSLGLENMNADSAVPGLNRDAAHARIVLIPRDKAEQRAIAHILGTLDDKIELNRKQSETLEAMA
RALFKAWFVDFEPVRAKMEGRWQRGQSLPGLPAHLYDLFPDRLVDSELGEIPEGWRVFAFGDVAQQGKGVVNPGNSPQDL
FTHYSLPAFDSAHCPSIEPGHAIKSNKTPVPDGAVLVSKLNPHIPRVWHVGTAGPNAVCSTEFIVWAPKAPANSAFLYCL
ASSPEFSGAMHQLVTGTSNSHQRVKPDQLREIRVFAATENAIEAFSEWVRSPLEKILQNRQQSRTLAQLRDALLPRLISG
ELRIADAEKPMEKFSQ

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 45856; Mature: 45724

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGEWIDTTVGEIAPFSYGKGLPERERKQTGSVPVYGSNGIVGFHDSALTGGPTIVIGRK
CCCCCHHCHHHHHCCCCCCCCCCHHHHHHCCCCEEECCCCEEEECCCCCCCCCEEEEECC
GTVGAVHYSPIPCWPIDTTFFVSDSDRSLVRYSYYLLKSLGLENMNADSAVPGLNRDAAH
CCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARIVLIPRDKAEQRAIAHILGTLDDKIELNRKQSETLEAMARALFKAWFVDFEPVRAKME
EEEEEEECCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GRWQRGQSLPGLPAHLYDLFPDRLVDSELGEIPEGWRVFAFGDVAQQGKGVVNPGNSPQD
CHHHCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCCCCCCHHH
LFTHYSLPAFDSAHCPSIEPGHAIKSNKTPVPDGAVLVSKLNPHIPRVWHVGTAGPNAVC
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCEE
STEFIVWAPKAPANSAFLYCLASSPEFSGAMHQLVTGTSNSHQRVKPDQLREIRVFAATE
CCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHEEEEEEECH
NAIEAFSEWVRSPLEKILQNRQQSRTLAQLRDALLPRLISGELRIADAEKPMEKFSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHCC
>Mature Secondary Structure 
AGEWIDTTVGEIAPFSYGKGLPERERKQTGSVPVYGSNGIVGFHDSALTGGPTIVIGRK
CCCCHHCHHHHHCCCCCCCCCCHHHHHHCCCCEEECCCCEEEECCCCCCCCCEEEEECC
GTVGAVHYSPIPCWPIDTTFFVSDSDRSLVRYSYYLLKSLGLENMNADSAVPGLNRDAAH
CCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARIVLIPRDKAEQRAIAHILGTLDDKIELNRKQSETLEAMARALFKAWFVDFEPVRAKME
EEEEEEECCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GRWQRGQSLPGLPAHLYDLFPDRLVDSELGEIPEGWRVFAFGDVAQQGKGVVNPGNSPQD
CHHHCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCCCCCCCHHH
LFTHYSLPAFDSAHCPSIEPGHAIKSNKTPVPDGAVLVSKLNPHIPRVWHVGTAGPNAVC
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCEE
STEFIVWAPKAPANSAFLYCLASSPEFSGAMHQLVTGTSNSHQRVKPDQLREIRVFAATE
CCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHEEEEEEECH
NAIEAFSEWVRSPLEKILQNRQQSRTLAQLRDALLPRLISGELRIADAEKPMEKFSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]