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The map label for this gene is thiC

Identifier: 94986024

GI number: 94986024

Start: 2026201

End: 2028030

Strand: Reverse

Name: thiC

Synonym: Dgeo_1924

Alternate gene names: 94986024

Gene position: 2028030-2026201 (Counterclockwise)

Preceding gene: 94986026

Following gene: 94986023

Centisome position: 82.2

GC content: 67.32

Gene sequence:

>1830_bases
ATGTCGGCACCCACTGCCCCCCAAACCACCCTGAGCACGGCGCCCTTTCCCAACAGCGAGAAGCGTTACCTGATCGGCAC
TCTGTATCCGCAGGTGCGGGTGCCTGTGCGCGCGATCCGGCAGTCTGCCACGCTGGAAATGATCGGTGGCCTGACCCGCC
AGCGCCCCAACCCCGACGTGCTGGTGCCGGATACCAGCGGCCCCTACACGGATCCCCGCATTCACATTGACCTGCGCCGG
GGGCTTCCGCACGCCCGGCCCTGGCTGGCCGCGGACGCCCGACTGGAGGTGCAGGCCGAGCGTCTGTCTGCCCCTCTTGA
TGGTGCAGGACCGCTGCCCTTTCCCGCCGTGCCGCTGTCCCGGCGTGCGCGGAGCGGGCAGGCGATCACCCAAATGCAGG
CTGCTCGCCGGGGCGAGATCACGCCGGAGATGGAGTTTGTGGCCCTGCGCGAGAACCTGCGGCAGGCCGAAGCGTTCGAG
TTGGCCCAGCAGCACCCCGGCCAGAGCTTCGGCGCGGCCATTCCGCGTGAGATCACCCCGGAATTTGTGCGCGCCGAGGT
GGCGCGGGGTCGGGCCGTGATTCCGGCCAACGTGAACCACCCCGAACTCGAACCGACGATCATCGGGCGCAACTTCCGCG
TAAAGGTCAACGCGAACCTCGGCACCAGCATCGTGACCAGCAGCATCGAGGAGGAAGTTGAAAAGATGGTCTGGGCGACC
CGCTGGGGGGCCGATACCGTGATGGACCTCTCCACCGGGCGGCACATCCATCAGACACGCGAGTGGATCCTCCGGAATAG
CCCCGTGCCGGTCGGCACCGTGCCCATCTATCAGGCGCTGGAGAAGGTGGGCGGCGTGGCCGAGGAGCTGACTTGGGAGG
TGTACCGGGACACCCTCATCGAACAGGCCGAGCAGGGGGTGGATTACTTCACCGTCCACGCGGGCGTGCGTCTGGCGCAC
ATTCCGCTCACGGCACGGCGGCGGACCGGTATCGTCTCACGCGGCGGCAGCATTCTGGCCAAGTGGTGCCTCGCCCACCA
CCGCGAGAACTTCCTCTACACCCACTTCCGGGAAATCTGCGAGATTCTGAGCGCCTACGACATCACCTTCAGCCTGGGTG
ACGGTCTGCGTCCCGGTTCGATCGAGGACGCCAACGACGCCGCCCAGTTTGCGGAACTGGACACGCTGGGCGAACTCACC
CGTGTGGCCTGGGAACACGGCGTGCAGACGATGATCGAGGGGCCGGGGCACGTGCCCATGCAGCTGATTCGCGAGAACAT
GACCCGGCAGCTGCAGGTCTGCCAGGAGGCGCCCTTCTACACGCTGGGGCCGCTCACCACCGACATTGCGCCCGGCTACG
ACCACATCACCTCGGCCATCGGTGCGGCGCAGATCGCCTGGTACGGCACCGCCATGCTGTGCTACGTGACGCCCAAGGAG
CACCTGGGCCTGCCCGACCGCCAGGACGTGCGTGACGGCGTGATCGCCTACCGCATCGCCGCGCACGCCGCCGATCTCGC
CAAGGGGCACCCCGGCGCACAGGCCCGTGACAACGCCCTGTCTCAGGCCAGATTTGAGTTTCGCTGGGAGGATCAATTCA
ATCTCGCCCTCGACCCCGAGCGTGCCCGCGCCCTTCACGACGCGACGCTGCCTGCCGACGCCGCCAAGACCGCCCATTTC
TGCTCGATGTGTGGCCCCCACTTTTGCTCCATGAAGCTCAGCCACGACCTGCGCGCGGACGACATCCTGAACGGCATGGC
GGAAAAAGCCCGCGAGTTTCGCGCGGCGGGCGGAGAACTCTACCTGGACCGCCCGGAGGGGACGCCGTGA

Upstream 100 bases:

>100_bases
ACCTAACCAGCTTCTGAACGCCAAGCAGCTTTGCCGCTTCGGGCCGCGCGAGCGCCCGGAGGTCCTGCTCCTTTCCTGCT
CTTTTGCCTGGAGGTTTTCG

Downstream 100 bases:

>100_bases
GCCGTTCCCTGGGCCGCCTGTACCTCGTCGCCACGCCCCGCCCGGATCAACCCGAAGCCGAGTTCCTGGCCCGGGTGGAG
GCGGCCCTCGACGGCGGCGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MSAPTAPQTTLSTAPFPNSEKRYLIGTLYPQVRVPVRAIRQSATLEMIGGLTRQRPNPDVLVPDTSGPYTDPRIHIDLRR
GLPHARPWLAADARLEVQAERLSAPLDGAGPLPFPAVPLSRRARSGQAITQMQAARRGEITPEMEFVALRENLRQAEAFE
LAQQHPGQSFGAAIPREITPEFVRAEVARGRAVIPANVNHPELEPTIIGRNFRVKVNANLGTSIVTSSIEEEVEKMVWAT
RWGADTVMDLSTGRHIHQTREWILRNSPVPVGTVPIYQALEKVGGVAEELTWEVYRDTLIEQAEQGVDYFTVHAGVRLAH
IPLTARRRTGIVSRGGSILAKWCLAHHRENFLYTHFREICEILSAYDITFSLGDGLRPGSIEDANDAAQFAELDTLGELT
RVAWEHGVQTMIEGPGHVPMQLIRENMTRQLQVCQEAPFYTLGPLTTDIAPGYDHITSAIGAAQIAWYGTAMLCYVTPKE
HLGLPDRQDVRDGVIAYRIAAHAADLAKGHPGAQARDNALSQARFEFRWEDQFNLALDPERARALHDATLPADAAKTAHF
CSMCGPHFCSMKLSHDLRADDILNGMAEKAREFRAAGGELYLDRPEGTP

Sequences:

>Translated_609_residues
MSAPTAPQTTLSTAPFPNSEKRYLIGTLYPQVRVPVRAIRQSATLEMIGGLTRQRPNPDVLVPDTSGPYTDPRIHIDLRR
GLPHARPWLAADARLEVQAERLSAPLDGAGPLPFPAVPLSRRARSGQAITQMQAARRGEITPEMEFVALRENLRQAEAFE
LAQQHPGQSFGAAIPREITPEFVRAEVARGRAVIPANVNHPELEPTIIGRNFRVKVNANLGTSIVTSSIEEEVEKMVWAT
RWGADTVMDLSTGRHIHQTREWILRNSPVPVGTVPIYQALEKVGGVAEELTWEVYRDTLIEQAEQGVDYFTVHAGVRLAH
IPLTARRRTGIVSRGGSILAKWCLAHHRENFLYTHFREICEILSAYDITFSLGDGLRPGSIEDANDAAQFAELDTLGELT
RVAWEHGVQTMIEGPGHVPMQLIRENMTRQLQVCQEAPFYTLGPLTTDIAPGYDHITSAIGAAQIAWYGTAMLCYVTPKE
HLGLPDRQDVRDGVIAYRIAAHAADLAKGHPGAQARDNALSQARFEFRWEDQFNLALDPERARALHDATLPADAAKTAHF
CSMCGPHFCSMKLSHDLRADDILNGMAEKAREFRAAGGELYLDRPEGTP
>Mature_608_residues
SAPTAPQTTLSTAPFPNSEKRYLIGTLYPQVRVPVRAIRQSATLEMIGGLTRQRPNPDVLVPDTSGPYTDPRIHIDLRRG
LPHARPWLAADARLEVQAERLSAPLDGAGPLPFPAVPLSRRARSGQAITQMQAARRGEITPEMEFVALRENLRQAEAFEL
AQQHPGQSFGAAIPREITPEFVRAEVARGRAVIPANVNHPELEPTIIGRNFRVKVNANLGTSIVTSSIEEEVEKMVWATR
WGADTVMDLSTGRHIHQTREWILRNSPVPVGTVPIYQALEKVGGVAEELTWEVYRDTLIEQAEQGVDYFTVHAGVRLAHI
PLTARRRTGIVSRGGSILAKWCLAHHRENFLYTHFREICEILSAYDITFSLGDGLRPGSIEDANDAAQFAELDTLGELTR
VAWEHGVQTMIEGPGHVPMQLIRENMTRQLQVCQEAPFYTLGPLTTDIAPGYDHITSAIGAAQIAWYGTAMLCYVTPKEH
LGLPDRQDVRDGVIAYRIAAHAADLAKGHPGAQARDNALSQARFEFRWEDQFNLALDPERARALHDATLPADAAKTAHFC
SMCGPHFCSMKLSHDLRADDILNGMAEKAREFRAAGGELYLDRPEGTP

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=613, Percent_Identity=66.721044045677, Blast_Score=833, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_DEIGD (Q1IX15)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605388.1
- STRING:   Q1IX15
- GeneID:   4057672
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_1924
- NMPDR:   fig|68909.1.peg.134
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q1IX15
- ProtClustDB:   PRK09284
- BioCyc:   DGEO319795:DGEO_1924-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 67342; Mature: 67211

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: BINDING 219-219 BINDING 248-248 BINDING 277-277 BINDING 313-313 BINDING 413-413 BINDING 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTAPQTTLSTAPFPNSEKRYLIGTLYPQVRVPVRAIRQSATLEMIGGLTRQRPNPDV
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCE
LVPDTSGPYTDPRIHIDLRRGLPHARPWLAADARLEVQAERLSAPLDGAGPLPFPAVPLS
EECCCCCCCCCCEEEEEHHHCCCCCCCCEECCCEEEEEHHHHCCCCCCCCCCCCCCCCHH
RRARSGQAITQMQAARRGEITPEMEFVALRENLRQAEAFELAQQHPGQSFGAAIPREITP
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
EFVRAEVARGRAVIPANVNHPELEPTIIGRNFRVKVNANLGTSIVTSSIEEEVEKMVWAT
HHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RWGADTVMDLSTGRHIHQTREWILRNSPVPVGTVPIYQALEKVGGVAEELTWEVYRDTLI
CCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EQAEQGVDYFTVHAGVRLAHIPLTARRRTGIVSRGGSILAKWCLAHHRENFLYTHFREIC
HHHHCCCCEEEEECCCEEEECCCHHHHHCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
EILSAYDITFSLGDGLRPGSIEDANDAAQFAELDTLGELTRVAWEHGVQTMIEGPGHVPM
HHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHH
QLIRENMTRQLQVCQEAPFYTLGPLTTDIAPGYDHITSAIGAAQIAWYGTAMLCYVTPKE
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHH
HLGLPDRQDVRDGVIAYRIAAHAADLAKGHPGAQARDNALSQARFEFRWEDQFNLALDPE
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCEEECHH
RARALHDATLPADAAKTAHFCSMCGPHFCSMKLSHDLRADDILNGMAEKAREFRAAGGEL
HHHHHHHCCCCCHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
YLDRPEGTP
EEECCCCCC
>Mature Secondary Structure 
SAPTAPQTTLSTAPFPNSEKRYLIGTLYPQVRVPVRAIRQSATLEMIGGLTRQRPNPDV
CCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCE
LVPDTSGPYTDPRIHIDLRRGLPHARPWLAADARLEVQAERLSAPLDGAGPLPFPAVPLS
EECCCCCCCCCCEEEEEHHHCCCCCCCCEECCCEEEEEHHHHCCCCCCCCCCCCCCCCHH
RRARSGQAITQMQAARRGEITPEMEFVALRENLRQAEAFELAQQHPGQSFGAAIPREITP
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
EFVRAEVARGRAVIPANVNHPELEPTIIGRNFRVKVNANLGTSIVTSSIEEEVEKMVWAT
HHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RWGADTVMDLSTGRHIHQTREWILRNSPVPVGTVPIYQALEKVGGVAEELTWEVYRDTLI
CCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EQAEQGVDYFTVHAGVRLAHIPLTARRRTGIVSRGGSILAKWCLAHHRENFLYTHFREIC
HHHHCCCCEEEEECCCEEEECCCHHHHHCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
EILSAYDITFSLGDGLRPGSIEDANDAAQFAELDTLGELTRVAWEHGVQTMIEGPGHVPM
HHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHH
QLIRENMTRQLQVCQEAPFYTLGPLTTDIAPGYDHITSAIGAAQIAWYGTAMLCYVTPKE
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHH
HLGLPDRQDVRDGVIAYRIAAHAADLAKGHPGAQARDNALSQARFEFRWEDQFNLALDPE
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEECCCCCEEECHH
RARALHDATLPADAAKTAHFCSMCGPHFCSMKLSHDLRADDILNGMAEKAREFRAAGGEL
HHHHHHHCCCCCHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
YLDRPEGTP
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA