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The map label for this gene is thiE

Identifier: 94986023

GI number: 94986023

Start: 2025539

End: 2026204

Strand: Reverse

Name: thiE

Synonym: Dgeo_1923

Alternate gene names: 94986023

Gene position: 2026204-2025539 (Counterclockwise)

Preceding gene: 94986024

Following gene: 94986022

Centisome position: 82.13

GC content: 74.32

Gene sequence:

>666_bases
GTGAGCCGTTCCCTGGGCCGCCTGTACCTCGTCGCCACGCCCCGCCCGGATCAACCCGAAGCCGAGTTCCTGGCCCGGGT
GGAGGCGGCCCTCGACGGCGGCGTGGACACGCTGCAACTGCGCTGCAAGGCGGGTTCGCCCCAGTACGGGGAGGCGCGAC
CGTACATCGCCCTGGCACGGCGGGTGGGGGCGCTCGCCCAGGCCCGCGACGTGCCCTTCTTCGTCAATGACCGGGTGGAT
GTGGCGCTCGCGAGCGGGGCGGACGGGGTGCATCTGGGCCAGGATGACCTGCCCGCCGAGTGGGCCCGCCGCCTCGCGCC
CGGTCTGCAGATCGGCCTCAGCACCCACTCGCCCGAGCAGGCGGCGCGGGCGCAGGCGGAGCATCCCACCTATTTTGCCG
TCGGTCCCGTGTACGCCACGCCCACCAAACCGGGCCGGGCCGCTGCCGGTCTGGCGTACGTGCGGCACGTCGCGGCCCTC
TGTCCCGCTGTGCCCTGGTACGCCATCGGTGGCGTGGACCTGTCCACCGTGGACGACGTGGTGGCGGCGGGCGCGACCCG
CGTCGCGGTTGTTCGGGCCGTGCTCGACGCCCCTGACCCCGCGCGGGCTGCTGCGGACCTGCTCGCTCGCCTGCCGGACG
CCTGGGAGGCCAGGGTATGCAGGTGA

Upstream 100 bases:

>100_bases
ACCTGCGCGCGGACGACATCCTGAACGGCATGGCGGAAAAAGCCCGCGAGTTTCGCGCGGCGGGCGGAGAACTCTACCTG
GACCGCCCGGAGGGGACGCC

Downstream 100 bases:

>100_bases
ACGGCCAGCCCTACCCGTACCGTGAGGGCCTCACCCTGCACGACCTGCTGCGCGAGCTGAACGTGTCGCCCGAACGGGTG
GCCGTCGCGGTGGGCGACGA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSRSLGRLYLVATPRPDQPEAEFLARVEAALDGGVDTLQLRCKAGSPQYGEARPYIALARRVGALAQARDVPFFVNDRVD
VALASGADGVHLGQDDLPAEWARRLAPGLQIGLSTHSPEQAARAQAEHPTYFAVGPVYATPTKPGRAAAGLAYVRHVAAL
CPAVPWYAIGGVDLSTVDDVVAAGATRVAVVRAVLDAPDPARAAADLLARLPDAWEARVCR

Sequences:

>Translated_221_residues
MSRSLGRLYLVATPRPDQPEAEFLARVEAALDGGVDTLQLRCKAGSPQYGEARPYIALARRVGALAQARDVPFFVNDRVD
VALASGADGVHLGQDDLPAEWARRLAPGLQIGLSTHSPEQAARAQAEHPTYFAVGPVYATPTKPGRAAAGLAYVRHVAAL
CPAVPWYAIGGVDLSTVDDVVAAGATRVAVVRAVLDAPDPARAAADLLARLPDAWEARVCR
>Mature_220_residues
SRSLGRLYLVATPRPDQPEAEFLARVEAALDGGVDTLQLRCKAGSPQYGEARPYIALARRVGALAQARDVPFFVNDRVDV
ALASGADGVHLGQDDLPAEWARRLAPGLQIGLSTHSPEQAARAQAEHPTYFAVGPVYATPTKPGRAAAGLAYVRHVAALC
PAVPWYAIGGVDLSTVDDVVAAGATRVAVVRAVLDAPDPARAAADLLARLPDAWEARVCR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=195, Percent_Identity=39.4871794871795, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=229, Percent_Identity=33.6244541484716, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_DEIGD (Q1IX16)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605387.1
- ProteinModelPortal:   Q1IX16
- STRING:   Q1IX16
- GeneID:   4057671
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_1923
- NMPDR:   fig|68909.1.peg.135
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- PhylomeDB:   Q1IX16
- ProtClustDB:   CLSK445781
- BioCyc:   DGEO319795:DGEO_1923-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23291; Mature: 23159

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSLGRLYLVATPRPDQPEAEFLARVEAALDGGVDTLQLRCKAGSPQYGEARPYIALAR
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHH
RVGALAQARDVPFFVNDRVDVALASGADGVHLGQDDLPAEWARRLAPGLQIGLSTHSPEQ
HHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCHHH
AARAQAEHPTYFAVGPVYATPTKPGRAAAGLAYVRHVAALCPAVPWYAIGGVDLSTVDDV
HHHHHCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
VAAGATRVAVVRAVLDAPDPARAAADLLARLPDAWEARVCR
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure 
SRSLGRLYLVATPRPDQPEAEFLARVEAALDGGVDTLQLRCKAGSPQYGEARPYIALAR
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHH
RVGALAQARDVPFFVNDRVDVALASGADGVHLGQDDLPAEWARRLAPGLQIGLSTHSPEQ
HHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCHHH
AARAQAEHPTYFAVGPVYATPTKPGRAAAGLAYVRHVAALCPAVPWYAIGGVDLSTVDDV
HHHHHCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
VAAGATRVAVVRAVLDAPDPARAAADLLARLPDAWEARVCR
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA