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The map label for this gene is thiG

Identifier: 94986021

GI number: 94986021

Start: 2024549

End: 2025352

Strand: Reverse

Name: thiG

Synonym: Dgeo_1921

Alternate gene names: 94986021

Gene position: 2025352-2024549 (Counterclockwise)

Preceding gene: 94986022

Following gene: 94986020

Centisome position: 82.09

GC content: 70.9

Gene sequence:

>804_bases
ATGTTCCCGCAAGACACCCTCACCATCGGCGGCAAGGTCTTTACGTCGCGCCTGATGCTGGGCACCGGAAAATTCACGGA
CTTTCACGTGATGCGTGACGCCTTGGAGGCGAGCGGTACCCAAATCGTGACGGTGGCGATCCGCCGCGTCGAGCTGAAGG
CCCCCGGTCATGTGGGGCTGCTGGACGCCCTGGACCTCAGCCGCTATCAACTGCTGCCGAACACAGCGGGCTGCCGCACG
GCGGAGGAAGCCGTGCGCGTGGCCCGGCTGGCGCGGGCGGCGACGGGCGTGAATTGGGTGAAGCTGGAAGTGATCCCGGA
CCCCCGCTGGCTGCTCCCCGACCCCATCGGTACCCTGAAAGCCGCGGAGATCCTGGTGGGCGAGGGATTCACGGTCCTGC
CCTACGTGCAGCCGGACGCGGTGCTGGCCCGCGCGCTGGAGCGGGTGGGCTGCGCCACCGTGATGCCTCTTGCCAGTCCC
ATCGGCAGCGGGCGCGGCCTGCGGACGGGCGAACTGCTGCGGACGGTGCTTGATGGGGCGGGCGTGCCCATCGTCGTAGA
CGCGGGCTTAGGGGTTCCCAGCGACGCGGCCCAGGCGCTGGAAGCCGGGGCGGATGCGGTGCTGGTGAACACGGCGGTGG
CCGAGGCCCGCGACCCAGTGGGGATGGCCCAGGCCTTCGCATTGGGGGTGCAGGCAGGCCGCGCGGCGTTTCTGGCGGGC
CGCATGGCTGAGCGCACCCATGCCAGCCCCAGCAGTCCTGCCGCCGGGGTGCCGTGCCTGCCCGACCCGGAGGTGCCCGT
GTGA

Upstream 100 bases:

>100_bases
GCGGTGGGCGACGACTTCTATCCCGGCGCGCGCATCCCCGACCGGCCCCTGGAAGAACAGGATGTCATCGAAATCGTCCG
CATCATCGGAGGAGGTTGAG

Downstream 100 bases:

>100_bases
AGGGGCAGGCTGCGAAAAACGCTCTGCTGTTCCCCGGCTGGCAGGCTGGCGGAGAAGGGCGGTGACGCCCGTGAGCCGCG
CACGGGAAGTGATTGTGGTG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MFPQDTLTIGGKVFTSRLMLGTGKFTDFHVMRDALEASGTQIVTVAIRRVELKAPGHVGLLDALDLSRYQLLPNTAGCRT
AEEAVRVARLARAATGVNWVKLEVIPDPRWLLPDPIGTLKAAEILVGEGFTVLPYVQPDAVLARALERVGCATVMPLASP
IGSGRGLRTGELLRTVLDGAGVPIVVDAGLGVPSDAAQALEAGADAVLVNTAVAEARDPVGMAQAFALGVQAGRAAFLAG
RMAERTHASPSSPAAGVPCLPDPEVPV

Sequences:

>Translated_267_residues
MFPQDTLTIGGKVFTSRLMLGTGKFTDFHVMRDALEASGTQIVTVAIRRVELKAPGHVGLLDALDLSRYQLLPNTAGCRT
AEEAVRVARLARAATGVNWVKLEVIPDPRWLLPDPIGTLKAAEILVGEGFTVLPYVQPDAVLARALERVGCATVMPLASP
IGSGRGLRTGELLRTVLDGAGVPIVVDAGLGVPSDAAQALEAGADAVLVNTAVAEARDPVGMAQAFALGVQAGRAAFLAG
RMAERTHASPSSPAAGVPCLPDPEVPV
>Mature_267_residues
MFPQDTLTIGGKVFTSRLMLGTGKFTDFHVMRDALEASGTQIVTVAIRRVELKAPGHVGLLDALDLSRYQLLPNTAGCRT
AEEAVRVARLARAATGVNWVKLEVIPDPRWLLPDPIGTLKAAEILVGEGFTVLPYVQPDAVLARALERVGCATVMPLASP
IGSGRGLRTGELLRTVLDGAGVPIVVDAGLGVPSDAAQALEAGADAVLVNTAVAEARDPVGMAQAFALGVQAGRAAFLAG
RMAERTHASPSSPAAGVPCLPDPEVPV

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=55.6, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_DEIGD (Q1IX18)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605385.1
- ProteinModelPortal:   Q1IX18
- SMR:   Q1IX18
- STRING:   Q1IX18
- GeneID:   4057669
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_1921
- NMPDR:   fig|68909.1.peg.137
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q1IX18
- ProtClustDB:   PRK00208
- BioCyc:   DGEO319795:DGEO_1921-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27679; Mature: 27679

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: ACT_SITE 101-101 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPQDTLTIGGKVFTSRLMLGTGKFTDFHVMRDALEASGTQIVTVAIRRVELKAPGHVGL
CCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCH
LDALDLSRYQLLPNTAGCRTAEEAVRVARLARAATGVNWVKLEVIPDPRWLLPDPIGTLK
HHHHCCHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHH
AAEILVGEGFTVLPYVQPDAVLARALERVGCATVMPLASPIGSGRGLRTGELLRTVLDGA
HHHEEECCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
GVPIVVDAGLGVPSDAAQALEAGADAVLVNTAVAEARDPVGMAQAFALGVQAGRAAFLAG
CCCEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHCHHHHHHH
RMAERTHASPSSPAAGVPCLPDPEVPV
HHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPQDTLTIGGKVFTSRLMLGTGKFTDFHVMRDALEASGTQIVTVAIRRVELKAPGHVGL
CCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCH
LDALDLSRYQLLPNTAGCRTAEEAVRVARLARAATGVNWVKLEVIPDPRWLLPDPIGTLK
HHHHCCHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHH
AAEILVGEGFTVLPYVQPDAVLARALERVGCATVMPLASPIGSGRGLRTGELLRTVLDGA
HHHEEECCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
GVPIVVDAGLGVPSDAAQALEAGADAVLVNTAVAEARDPVGMAQAFALGVQAGRAAFLAG
CCCEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHCHHHHHHH
RMAERTHASPSSPAAGVPCLPDPEVPV
HHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA