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The map label for this gene is dadA [C]

Identifier: 94986020

GI number: 94986020

Start: 2023570

End: 2024487

Strand: Reverse

Name: dadA [C]

Synonym: Dgeo_1920

Alternate gene names: 94986020

Gene position: 2024487-2023570 (Counterclockwise)

Preceding gene: 94986021

Following gene: 94986019

Centisome position: 82.06

GC content: 72.44

Gene sequence:

>918_bases
GTGACGCCCGTGAGCCGCGCACGGGAAGTGATTGTGGTGGGCGGCGGTCTGATCGGGTCGCTCGTGGCGTTCACGTTGCG
GCAGGCAGGTGCCGATGTGCTGGTGCTCGATGCCGATCGGTGCGGGGCGGCGTGGCGTGCGGCAGCGGGCCTCCTGACGC
CAGATGGGGAGCGGTTACGCGGTCCGTTGCACGGGGCTGCCCTGGAGAGCCTGCGGCGCTGGCCTGCCCTGGTGGCACAA
CTCGAACGTTCGGGGGTACCGGTGTCGTTCCGGGCGGGCGTGGCGCGGATGCAACCTGGTGGCGGCGTGCAGACCACGCC
GGGCGAGGGCAGCCTGCATCCGCCGTCGGTGGTGCGGGCCGCTCGACAGGCGCTGGAGGTGGTGACCGAGCACGTGCACG
CCCTGGTGCCGGGTGGTTGTGGCGTGCGGGTGCGAACAGCTGCGGGCGATTGGCGCGCCCAGCGGGTGGTGCTTGCCGCC
GGCGCGTGGAGTGGTGCGTTTGGTGTGCCGGTCACGGCGCAGCGGGGGCAAGCGCTCCTGCTGCGGGCCGTGCCGGACGT
GGGGGCGCGCTATGGGCCGCCCACGCGGGGCTTTTCCCGCTATGCCCTGTCTCGTCCGGATGGCCTCTACATTGGTGCCA
CAAGTGCCCGGGGCTGGTCAGTGACGCCTGATCCACGTGCTGCGTGCTGGCTGCGCGGCGTGGCACGGAGGATCGTGCCG
GAAGCGAATGGCGCCGAAGTCGCCGCCCACCTGGTGGGCTTGCGCCCGGTGACGCCAGATGGCCTGCCGCTGGTGGCGCC
GCACCCCACGCTTCCGGGTGTGTTCGTCGCGACGGGGCACGGGCGGCACGGCGCGTTGCTCGCTCCGGTCACGGCGGCAC
GCATCGTGGGCCTGTTGGAACAGGGTGTGAGCGCATGA

Upstream 100 bases:

>100_bases
GGGGTGCCGTGCCTGCCCGACCCGGAGGTGCCCGTGTGAAGGGGCAGGCTGCGAAAAACGCTCTGCTGTTCCCCGGCTGG
CAGGCTGGCGGAGAAGGGCG

Downstream 100 bases:

>100_bases
CCGTTCCCGTTGCTCTCACCATCGCTGGCTCCGACTCCGGGGGCGGGGCGGGGATCCAGGCGGACCTCAAGACCTTTGAG
GCGCATGGCGTCTTCGGCAC

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTPVSRAREVIVVGGGLIGSLVAFTLRQAGADVLVLDADRCGAAWRAAAGLLTPDGERLRGPLHGAALESLRRWPALVAQ
LERSGVPVSFRAGVARMQPGGGVQTTPGEGSLHPPSVVRAARQALEVVTEHVHALVPGGCGVRVRTAAGDWRAQRVVLAA
GAWSGAFGVPVTAQRGQALLLRAVPDVGARYGPPTRGFSRYALSRPDGLYIGATSARGWSVTPDPRAACWLRGVARRIVP
EANGAEVAAHLVGLRPVTPDGLPLVAPHPTLPGVFVATGHGRHGALLAPVTAARIVGLLEQGVSA

Sequences:

>Translated_305_residues
MTPVSRAREVIVVGGGLIGSLVAFTLRQAGADVLVLDADRCGAAWRAAAGLLTPDGERLRGPLHGAALESLRRWPALVAQ
LERSGVPVSFRAGVARMQPGGGVQTTPGEGSLHPPSVVRAARQALEVVTEHVHALVPGGCGVRVRTAAGDWRAQRVVLAA
GAWSGAFGVPVTAQRGQALLLRAVPDVGARYGPPTRGFSRYALSRPDGLYIGATSARGWSVTPDPRAACWLRGVARRIVP
EANGAEVAAHLVGLRPVTPDGLPLVAPHPTLPGVFVATGHGRHGALLAPVTAARIVGLLEQGVSA
>Mature_304_residues
TPVSRAREVIVVGGGLIGSLVAFTLRQAGADVLVLDADRCGAAWRAAAGLLTPDGERLRGPLHGAALESLRRWPALVAQL
ERSGVPVSFRAGVARMQPGGGVQTTPGEGSLHPPSVVRAARQALEVVTEHVHALVPGGCGVRVRTAAGDWRAQRVVLAAG
AWSGAFGVPVTAQRGQALLLRAVPDVGARYGPPTRGFSRYALSRPDGLYIGATSARGWSVTPDPRAACWLRGVARRIVPE
ANGAEVAAHLVGLRPVTPDGLPLVAPHPTLPGVFVATGHGRHGALLAPVTAARIVGLLEQGVSA

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 31404; Mature: 31272

Theoretical pI: Translated: 11.87; Mature: 11.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVSRAREVIVVGGGLIGSLVAFTLRQAGADVLVLDADRCGAAWRAAAGLLTPDGERLR
CCCHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCHHHHC
GPLHGAALESLRRWPALVAQLERSGVPVSFRAGVARMQPGGGVQTTPGEGSLHPPSVVRA
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCEEECCCCCCCCCCCCCCCCCHHHHHH
ARQALEVVTEHVHALVPGGCGVRVRTAAGDWRAQRVVLAAGAWSGAFGVPVTAQRGQALL
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCEECCCCCEEE
LRAVPDVGARYGPPTRGFSRYALSRPDGLYIGATSARGWSVTPDPRAACWLRGVARRIVP
EEECCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
EANGAEVAAHLVGLRPVTPDGLPLVAPHPTLPGVFVATGHGRHGALLAPVTAARIVGLLE
CCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHH
QGVSA
HCCCC
>Mature Secondary Structure 
TPVSRAREVIVVGGGLIGSLVAFTLRQAGADVLVLDADRCGAAWRAAAGLLTPDGERLR
CCHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCHHHHC
GPLHGAALESLRRWPALVAQLERSGVPVSFRAGVARMQPGGGVQTTPGEGSLHPPSVVRA
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCEEECCCCCCCCCCCCCCCCCHHHHHH
ARQALEVVTEHVHALVPGGCGVRVRTAAGDWRAQRVVLAAGAWSGAFGVPVTAQRGQALL
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCEECCCCCEEE
LRAVPDVGARYGPPTRGFSRYALSRPDGLYIGATSARGWSVTPDPRAACWLRGVARRIVP
EEECCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
EANGAEVAAHLVGLRPVTPDGLPLVAPHPTLPGVFVATGHGRHGALLAPVTAARIVGLLE
CCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHH
QGVSA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]