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The map label for this gene is aceF [H]

Identifier: 94985986

GI number: 94985986

Start: 1981731

End: 1983515

Strand: Reverse

Name: aceF [H]

Synonym: Dgeo_1886

Alternate gene names: 94985986

Gene position: 1983515-1981731 (Counterclockwise)

Preceding gene: 94985987

Following gene: 94985982

Centisome position: 80.4

GC content: 68.35

Gene sequence:

>1785_bases
ATGGCAACAGAACTGAAACTTCCCGATGTGGGCGACAATATCGAGCAGGGGACGGTCGTCACGGTGCTGGTGAAGCCCGG
CGACCAGATCACAGAAGGGCAACCCGTCATCGAGATCGAGACCGATAAGGCGGTGGTGGAAGTCCCGGCCAGTGCAGGGG
GGATCGTCGAGGCGGTGCAGGTGAAGGTGGGAGACAGCGTGAAGGTGGGCGACGTGCTCCTGACGCTGGCCGGCACTGGG
GCTGCGGCGGCGCCGGCCAGTGCCCCCGGGAGCACCCCAGTTGCCCCATCCGCCGAAGCGGAAAGCAGCGCTGTTGCGTC
CGATCCCGCTGTGGCCAATCGCGTCGCGCAGGGGCAGCAGGCCGCACAGAAGGCGCAGGCAGCGTCCGGCAGCCAGCCGC
AGGCCCCGGTGGGTCAGCCCTCCGAACAGGCGGGCGGCACCGCCCAGGTCACTCTCCCCGACGTGGGCGACAACATCGAG
CAGGGGACGGTCGTCACGGTGTTGGTGAAGCCCGGCGACCAGATCACGGAAGGGCAGCCTGTCATCGAGATTGAAACGGA
CAAGGCGGTCGTCGAGGTGCCCTCCAGCGCGGGAGGAACCGTGCAGGACGTTCGGGTGAAGGTGGGGGACAGCGTGAAGG
TGGGTGACGTGCTCCTGACGCTGGTGGGCCAAACGGGCACGACCCAGGATCAGGGTGCTCAAGCGCCTGCGTCTCAGCCG
GCGCCCGCCGCGCCTGCGTCTCGGCCGGTGCAGCCGCCCGCCCAAGGCGCATTGGAACCCGGCAGCCTGACACCGACCGC
CCCGACGCAGGCCTCGGGTGCCCAGCGGCCCTACAACACCCAGACCTACGACGGCCGCCCGGTGATTCCCGCTGCGCCCA
GCGTGCGCCGTCTGGCCCGCGAGCTGCACGTGAACATTCAAGCCGTGCACGGCACCGGTATCGCGGGGCGCATCAGCGAG
GAGGACGTGCGCCGTGCGGCGGGGACGCCCAGCGTGCAGGCTCCTGCGGCCCAGGCCGCGCCGACCACCGCTGCTCCGGC
TCCCGCCGCCGCTGCCCAGCCCCTCCCCGACTTCACGAAGTGGGGTCCGGTGCGCCGCGAGGACATGAGCGGTATCCGCA
AGGCGACGGTGCGCTCCATGACCCAGTCGTGGACGACCATCCCGATGGTCACGCACTTCGACAAGGCCGATGTGACCCGC
ATGGAGGAGGTCCGCAAGGCGTTTGCCCCGCGGGTGGAGAAGGCGGGCGGCAAGCTCACCATGACCCACATCCTGATGAA
GGTGGTGGCGAACGCCCTGCGGAAATTCCCCAAGTTCGGAGCCAGCCTCGACCTCGAACACCAGCAGGTGATCTACAAGG
ACTACGTGAATCTCGGCGTGGCGGTCGACACGCCCCAGGGGCTGCTGGTGCCCGTTCTCAAGGACGCCGACCGCAAGAGC
ATCACCGAAATCGTGCTGGAGCTGAGTGAGCTGGCGGCCAAGGCGCGCGACCGCAAGCTGAGCCCAAGCGAGATGCAGGG
GGCGACCTTCACCATCTCCAACCTCGGCGGGATCGGCGGCACTGGCTTTACCCCGATCGTCAACGCGCCGGAGGTCGCCA
TCCTGGGGGTGTCGCGCGGCGGCTTCGAGCCGGTGTGGAACAAGGAGACGGGCAGCTTTGAGCCCCGCAATATGCTGCCC
CTCTCCCTCACCTATGACCACCGCCTGATCGATGGGGCGGATGCCGCCCGCTTCCTGCGGTACATCAGCGAGGCGCTGGA
AGACCCGTTCCTGATCTCGTTGTAA

Upstream 100 bases:

>100_bases
CGTTAACCCGTCAGCCATCAGCTTGTAGTGACCAGCCCTCTATGCTGAAGGCCGATCGCTGCCGGCTGATGGCCCTCTCA
ATCAAGGAGCGTGACTGCCC

Downstream 100 bases:

>100_bases
AGGGGAGTGAACGTTTAGCGGTCAGCAGCCAGCAAAATCAACCCCCGTCCGTGTGGCGGGGGTATTCGCTGATGGCTGCC
ATTACCGCGCGGTGTCGTCC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MATELKLPDVGDNIEQGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPASAGGIVEAVQVKVGDSVKVGDVLLTLAGTG
AAAAPASAPGSTPVAPSAEAESSAVASDPAVANRVAQGQQAAQKAQAASGSQPQAPVGQPSEQAGGTAQVTLPDVGDNIE
QGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPSSAGGTVQDVRVKVGDSVKVGDVLLTLVGQTGTTQDQGAQAPASQP
APAAPASRPVQPPAQGALEPGSLTPTAPTQASGAQRPYNTQTYDGRPVIPAAPSVRRLARELHVNIQAVHGTGIAGRISE
EDVRRAAGTPSVQAPAAQAAPTTAAPAPAAAAQPLPDFTKWGPVRREDMSGIRKATVRSMTQSWTTIPMVTHFDKADVTR
MEEVRKAFAPRVEKAGGKLTMTHILMKVVANALRKFPKFGASLDLEHQQVIYKDYVNLGVAVDTPQGLLVPVLKDADRKS
ITEIVLELSELAAKARDRKLSPSEMQGATFTISNLGGIGGTGFTPIVNAPEVAILGVSRGGFEPVWNKETGSFEPRNMLP
LSLTYDHRLIDGADAARFLRYISEALEDPFLISL

Sequences:

>Translated_594_residues
MATELKLPDVGDNIEQGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPASAGGIVEAVQVKVGDSVKVGDVLLTLAGTG
AAAAPASAPGSTPVAPSAEAESSAVASDPAVANRVAQGQQAAQKAQAASGSQPQAPVGQPSEQAGGTAQVTLPDVGDNIE
QGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPSSAGGTVQDVRVKVGDSVKVGDVLLTLVGQTGTTQDQGAQAPASQP
APAAPASRPVQPPAQGALEPGSLTPTAPTQASGAQRPYNTQTYDGRPVIPAAPSVRRLARELHVNIQAVHGTGIAGRISE
EDVRRAAGTPSVQAPAAQAAPTTAAPAPAAAAQPLPDFTKWGPVRREDMSGIRKATVRSMTQSWTTIPMVTHFDKADVTR
MEEVRKAFAPRVEKAGGKLTMTHILMKVVANALRKFPKFGASLDLEHQQVIYKDYVNLGVAVDTPQGLLVPVLKDADRKS
ITEIVLELSELAAKARDRKLSPSEMQGATFTISNLGGIGGTGFTPIVNAPEVAILGVSRGGFEPVWNKETGSFEPRNMLP
LSLTYDHRLIDGADAARFLRYISEALEDPFLISL
>Mature_593_residues
ATELKLPDVGDNIEQGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPASAGGIVEAVQVKVGDSVKVGDVLLTLAGTGA
AAAPASAPGSTPVAPSAEAESSAVASDPAVANRVAQGQQAAQKAQAASGSQPQAPVGQPSEQAGGTAQVTLPDVGDNIEQ
GTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPSSAGGTVQDVRVKVGDSVKVGDVLLTLVGQTGTTQDQGAQAPASQPA
PAAPASRPVQPPAQGALEPGSLTPTAPTQASGAQRPYNTQTYDGRPVIPAAPSVRRLARELHVNIQAVHGTGIAGRISEE
DVRRAAGTPSVQAPAAQAAPTTAAPAPAAAAQPLPDFTKWGPVRREDMSGIRKATVRSMTQSWTTIPMVTHFDKADVTRM
EEVRKAFAPRVEKAGGKLTMTHILMKVVANALRKFPKFGASLDLEHQQVIYKDYVNLGVAVDTPQGLLVPVLKDADRKSI
TEIVLELSELAAKARDRKLSPSEMQGATFTISNLGGIGGTGFTPIVNAPEVAILGVSRGGFEPVWNKETGSFEPRNMLPL
SLTYDHRLIDGADAARFLRYISEALEDPFLISL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=473, Percent_Identity=29.3868921775898, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI19923748, Length=235, Percent_Identity=39.5744680851064, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI31711992, Length=599, Percent_Identity=27.8797996661102, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI203098816, Length=481, Percent_Identity=28.0665280665281, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=27.8617710583153, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI260898739, Length=140, Percent_Identity=40.7142857142857, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1786305, Length=645, Percent_Identity=34.5736434108527, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=31.9101123595506, Blast_Score=191, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17537937, Length=445, Percent_Identity=29.438202247191, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=458, Percent_Identity=30.5676855895196, Blast_Score=165, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=225, Percent_Identity=40.4444444444444, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=225, Percent_Identity=33.7777777777778, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=458, Percent_Identity=28.6026200873362, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=27.9220779220779, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI18859875, Length=454, Percent_Identity=32.3788546255507, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI20129315, Length=450, Percent_Identity=30.2222222222222, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24582497, Length=440, Percent_Identity=30.2272727272727, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=38.1974248927039, Blast_Score=138, Evalue=9e-33,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 61587; Mature: 61456

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00120 LIPASE_SER ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATELKLPDVGDNIEQGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPASAGGIVEAVQ
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCCCCEEEEE
VKVGDSVKVGDVLLTLAGTGAAAAPASAPGSTPVAPSAEAESSAVASDPAVANRVAQGQQ
EECCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AAQKAQAASGSQPQAPVGQPSEQAGGTAQVTLPDVGDNIEQGTVVTVLVKPGDQITEGQP
HHHHHHHCCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCC
VIEIETDKAVVEVPSSAGGTVQDVRVKVGDSVKVGDVLLTLVGQTGTTQDQGAQAPASQP
EEEEECCCEEEECCCCCCCCEEEEEEEECCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
APAAPASRPVQPPAQGALEPGSLTPTAPTQASGAQRPYNTQTYDGRPVIPAAPSVRRLAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ELHVNIQAVHGTGIAGRISEEDVRRAAGTPSVQAPAAQAAPTTAAPAPAAAAQPLPDFTK
HHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WGPVRREDMSGIRKATVRSMTQSWTTIPMVTHFDKADVTRMEEVRKAFAPRVEKAGGKLT
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCH
MTHILMKVVANALRKFPKFGASLDLEHQQVIYKDYVNLGVAVDTPQGLLVPVLKDADRKS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEEHHHCCCCHHH
ITEIVLELSELAAKARDRKLSPSEMQGATFTISNLGGIGGTGFTPIVNAPEVAILGVSRG
HHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCC
GFEPVWNKETGSFEPRNMLPLSLTYDHRLIDGADAARFLRYISEALEDPFLISL
CCCCCCCCCCCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
ATELKLPDVGDNIEQGTVVTVLVKPGDQITEGQPVIEIETDKAVVEVPASAGGIVEAVQ
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCCCCEEEEE
VKVGDSVKVGDVLLTLAGTGAAAAPASAPGSTPVAPSAEAESSAVASDPAVANRVAQGQQ
EECCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AAQKAQAASGSQPQAPVGQPSEQAGGTAQVTLPDVGDNIEQGTVVTVLVKPGDQITEGQP
HHHHHHHCCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCC
VIEIETDKAVVEVPSSAGGTVQDVRVKVGDSVKVGDVLLTLVGQTGTTQDQGAQAPASQP
EEEEECCCEEEECCCCCCCCEEEEEEEECCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCC
APAAPASRPVQPPAQGALEPGSLTPTAPTQASGAQRPYNTQTYDGRPVIPAAPSVRRLAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ELHVNIQAVHGTGIAGRISEEDVRRAAGTPSVQAPAAQAAPTTAAPAPAAAAQPLPDFTK
HHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WGPVRREDMSGIRKATVRSMTQSWTTIPMVTHFDKADVTRMEEVRKAFAPRVEKAGGKLT
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCH
MTHILMKVVANALRKFPKFGASLDLEHQQVIYKDYVNLGVAVDTPQGLLVPVLKDADRKS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEEHHHCCCCHHH
ITEIVLELSELAAKARDRKLSPSEMQGATFTISNLGGIGGTGFTPIVNAPEVAILGVSRG
HHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCC
GFEPVWNKETGSFEPRNMLPLSLTYDHRLIDGADAARFLRYISEALEDPFLISL
CCCCCCCCCCCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]