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The map label for this gene is hrdB [H]

Identifier: 94985446

GI number: 94985446

Start: 1433106

End: 1434317

Strand: Reverse

Name: hrdB [H]

Synonym: Dgeo_1346

Alternate gene names: 94985446

Gene position: 1434317-1433106 (Counterclockwise)

Preceding gene: 94985447

Following gene: 94985445

Centisome position: 58.14

GC content: 64.36

Gene sequence:

>1212_bases
ATGGAGATGATTGCCATGCCTACCAAAGTTGCCCCCAATCGCCTGTCTGAGAAGACCTCGCCCCGGCGAGCACGCGCCCC
GAAAAGCGCACAGACGGCTCTTCTTGAACCTGAGGTCCAGGAGGACCGCCTTGACCTAGAGGCCGGGGCGGATCTCAATG
ACCTTGACCTGCTCCTGCTCCCCGGCGGGGCTGAGGCGCTTGACGAGGAGCTGCAGCTGGAGGATGAGGCGCCGGCAGTC
GCTGCGAATGCTGGCGACGAGGAGGCGTTTGCCCTCGAGGCGAGTCCGGCCCTCTCGCAGGGCGACTCGCTGCGGCAGTA
CCTGCACGAGATCGGCCGGGTGCCGCTGCTGACTGTGGCCGAGGAAATGGACCTTGCGCGCCGCTACGAGGAAGGCGAGG
CGGCCCGTCAGGCCCTCGACAGCAACCCGGATCTCGACGACCGGACCCGCCGCCAGCTGCAACGCCAGGTGGAGGACGGC
GCCGCCGCCAAACAGGCCCTGATCGAGGCGAACCTGCGCCTGGTCGTGAGCATTGCCAAGAGGTATACCAACCGGGGCCT
AGGTTTGCTTGACCTGATTCAGGAGGGCAACCAGGGCCTGATCCGCGCGGTGGAAAAGTTCGAGTACCGGCGCGGCTACA
AGTTCTCCACCTATGCGACCTGGTGGATTCGTCAGTCCCTCACCCGTGCGATTGCAGACAAATCGCGCACCATTCGCGTA
CCCGTCCACATGGTGGAGACCATCAACCGGCTGTCCCGGATGACGCGTCAGCTGGAGATGGAACTGTCGCGTGAGCCCTT
GCCGGCGGAGATCGCCGAGGCGATGGGGCCGGGTTGGGACGCTGCCAAGGTCGAGGAGACACAAAAGGTCGGCTGGGAGC
CGATGTCACTGGAGATGCCGATTGGTGAGGAGGGGGACAGCACCTACGGGGACTTTCTGCCCGACGATCGCTTTGCTTCT
CCGATACAAAACGCCAGTCAGGCGCTGCTGAGCGAGGCGCTGGAACGGGCCTTTGGGACGCTGAATGAGCGGGAGGCGTT
GGTGCTTCGGCTGCGCCACGGCTTTGTGGATGGGCATGAACACACCCTGGAAGAGGTGGGACAGCTGCTGAGCGTCACCC
GCGAGCGTGTGCGGCAGATCGAGAACAAGGCGCTGCGCAAACTCAAGTACCAAGAAGGGCTGACCCGCGGCCTGCGGGAC
TTCCTGGAATAA

Upstream 100 bases:

>100_bases
CCCGCGGACACGGGGCGAACCTGCTCAGCTGGACTCCCCTTCACCCTGCTTCACACCGCGCTTCACCCTGCCCGGACTAC
CGCTGCGGTCCGGAGAACCC

Downstream 100 bases:

>100_bases
GCCGGAATAAGTTGGGCAAAGACGGCCACGTTGAGCCCCCTCGCCTCTGGAGGCCGGCTTGTGCATTGGTGTGGTGGAGG
CGCACTGGCCCATGCCCGCC

Product: RNA polymerase, sigma 28 subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEMIAMPTKVAPNRLSEKTSPRRARAPKSAQTALLEPEVQEDRLDLEAGADLNDLDLLLLPGGAEALDEELQLEDEAPAV
AANAGDEEAFALEASPALSQGDSLRQYLHEIGRVPLLTVAEEMDLARRYEEGEAARQALDSNPDLDDRTRRQLQRQVEDG
AAAKQALIEANLRLVVSIAKRYTNRGLGLLDLIQEGNQGLIRAVEKFEYRRGYKFSTYATWWIRQSLTRAIADKSRTIRV
PVHMVETINRLSRMTRQLEMELSREPLPAEIAEAMGPGWDAAKVEETQKVGWEPMSLEMPIGEEGDSTYGDFLPDDRFAS
PIQNASQALLSEALERAFGTLNEREALVLRLRHGFVDGHEHTLEEVGQLLSVTRERVRQIENKALRKLKYQEGLTRGLRD
FLE

Sequences:

>Translated_403_residues
MEMIAMPTKVAPNRLSEKTSPRRARAPKSAQTALLEPEVQEDRLDLEAGADLNDLDLLLLPGGAEALDEELQLEDEAPAV
AANAGDEEAFALEASPALSQGDSLRQYLHEIGRVPLLTVAEEMDLARRYEEGEAARQALDSNPDLDDRTRRQLQRQVEDG
AAAKQALIEANLRLVVSIAKRYTNRGLGLLDLIQEGNQGLIRAVEKFEYRRGYKFSTYATWWIRQSLTRAIADKSRTIRV
PVHMVETINRLSRMTRQLEMELSREPLPAEIAEAMGPGWDAAKVEETQKVGWEPMSLEMPIGEEGDSTYGDFLPDDRFAS
PIQNASQALLSEALERAFGTLNEREALVLRLRHGFVDGHEHTLEEVGQLLSVTRERVRQIENKALRKLKYQEGLTRGLRD
FLE
>Mature_403_residues
MEMIAMPTKVAPNRLSEKTSPRRARAPKSAQTALLEPEVQEDRLDLEAGADLNDLDLLLLPGGAEALDEELQLEDEAPAV
AANAGDEEAFALEASPALSQGDSLRQYLHEIGRVPLLTVAEEMDLARRYEEGEAARQALDSNPDLDDRTRRQLQRQVEDG
AAAKQALIEANLRLVVSIAKRYTNRGLGLLDLIQEGNQGLIRAVEKFEYRRGYKFSTYATWWIRQSLTRAIADKSRTIRV
PVHMVETINRLSRMTRQLEMELSREPLPAEIAEAMGPGWDAAKVEETQKVGWEPMSLEMPIGEEGDSTYGDFLPDDRFAS
PIQNASQALLSEALERAFGTLNEREALVLRLRHGFVDGHEHTLEEVGQLLSVTRERVRQIENKALRKLKYQEGLTRGLRD
FLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=298, Percent_Identity=49.6644295302013, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1789098, Length=298, Percent_Identity=39.9328859060403, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788231, Length=239, Percent_Identity=29.7071129707113, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 45276; Mature: 45276

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMIAMPTKVAPNRLSEKTSPRRARAPKSAQTALLEPEVQEDRLDLEAGADLNDLDLLLL
CCCCCCCCCCCCHHHHHCCCCHHHCCCCHHHHHHCCCCHHHHCCCHHCCCCCCCCEEEEE
PGGAEALDEELQLEDEAPAVAANAGDEEAFALEASPALSQGDSLRQYLHEIGRVPLLTVA
CCCHHHHHHHHCCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHH
EEMDLARRYEEGEAARQALDSNPDLDDRTRRQLQRQVEDGAAAKQALIEANLRLVVSIAK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYTNRGLGLLDLIQEGNQGLIRAVEKFEYRRGYKFSTYATWWIRQSLTRAIADKSRTIRV
HHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
PVHMVETINRLSRMTRQLEMELSREPLPAEIAEAMGPGWDAAKVEETQKVGWEPMSLEMP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECC
IGEEGDSTYGDFLPDDRFASPIQNASQALLSEALERAFGTLNEREALVLRLRHGFVDGHE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
HTLEEVGQLLSVTRERVRQIENKALRKLKYQEGLTRGLRDFLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEMIAMPTKVAPNRLSEKTSPRRARAPKSAQTALLEPEVQEDRLDLEAGADLNDLDLLLL
CCCCCCCCCCCCHHHHHCCCCHHHCCCCHHHHHHCCCCHHHHCCCHHCCCCCCCCEEEEE
PGGAEALDEELQLEDEAPAVAANAGDEEAFALEASPALSQGDSLRQYLHEIGRVPLLTVA
CCCHHHHHHHHCCCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHH
EEMDLARRYEEGEAARQALDSNPDLDDRTRRQLQRQVEDGAAAKQALIEANLRLVVSIAK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYTNRGLGLLDLIQEGNQGLIRAVEKFEYRRGYKFSTYATWWIRQSLTRAIADKSRTIRV
HHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
PVHMVETINRLSRMTRQLEMELSREPLPAEIAEAMGPGWDAAKVEETQKVGWEPMSLEMP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECC
IGEEGDSTYGDFLPDDRFASPIQNASQALLSEALERAFGTLNEREALVLRLRHGFVDGHE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
HTLEEVGQLLSVTRERVRQIENKALRKLKYQEGLTRGLRDFLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]