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The map label for this gene is clpB [H]

Identifier: 94985317

GI number: 94985317

Start: 1293731

End: 1295968

Strand: Reverse

Name: clpB [H]

Synonym: Dgeo_1215

Alternate gene names: 94985317

Gene position: 1295968-1293731 (Counterclockwise)

Preceding gene: 94985318

Following gene: 94985316

Centisome position: 52.53

GC content: 66.31

Gene sequence:

>2238_bases
ATGAACAGATACGACGACCGTGCCCGCCTCGTGTTCCACTACGCCCGTGAAGAAGGCAACCGCCTCGGGCACGCGATGGT
CGGCCCCGAACACCTGTTGCTCGGTCTGATGCGCGAGGGCGGCACAGCCGCGACCATTCTCTCCGAGTTTGGTGCTTCGC
TCGACGGGTTGCGCCGCCGTGTCGAGGAGATCATCGGTCGGGGCGAGGGCAACCGTCTGAACGACGCCCCGTCCATTACC
CCTCGCGCCCGCCGCGTGATGGAGCTGGCCTCGGCCGAGGCCCGCTCGCTGGGCGCACAGGTTACCTCCACGGAACACAT
CCTGCTCGGCATCATCCGCGAGGGGGACGGGGTGGCCTTCCGCATCCTGCAAGAACTCACCAAAGACGTCGATACCATCC
GCTGGCGCGTGCTGGCGCAGGGAGACGGCAGCAGCGGCAAGGCCGCCAAGCCGGTTGCCACGCCCTTCCTCGACGAGTAC
GGCCGTGACCTCACCAAGCAAGCCCGCGAGGGCAAGCTCGACCCGGTGATCGGGCGAAGCGAGGAAATCCGGCGCGTGAC
CCAGATCCTCACGCGGCGCACCAAGAACAACCCGGTCCTGATCGGCGACCCGGGCGTGGGCAAGACCGCCATCGTCGAGG
GCCTGGCGCTCGCCATCTTTGAAAAGCGCACCCCGCCCAATCTGCACGGTGTCCGCCTGGTCAGCCTCGACCTCTCGGGC
GTCGTGGCGGGCACCAAGTACCGCGGCGAGTTTGAGGAGCGGCTGCGCCAGATCATCGAGGAACTGCGCAATGCCAAGGT
GATGGCCTTTATCGACGAGCTGCACACCCTGGTCGGCGCGGGCGGCGCAGAGGGGACGCTGGACGCGGCGAACATCCTCA
AGCCTGCCCTCTCGCGGGGTGAGATTCAGGTGATCGGCGCCACCACGACCGGCGAGTACCACCGCTACATCGAGAAGGAT
GCAGCCCTGGAACGCCGCTTCCAGCCGGTGATTGTGCTGGAACCCAGCCCAGCCGAGACGCTCCAGATCCTGCGCGGCCT
GCGTCCACGTTACGAGGAACACCACGGCGTCCAGATTCCTGAATCTGCGCTCGAGTTGGCGGTTCGCATCGGGGAACGCT
CGCTGCCGGGCCGCAACTTCCCGGACAAGGCCATTGACCTGATCGACGAGGCCGCCAGCCGCGTTCGATTGAACATGAGC
GTGGGCCTGCCCGTCTCAGAGACCGAGGACGGTGAGCCGATGGTGTCGCGCGAGGACATCGAGAGCGTGATCAACTCGAT
GGGCGGCATCTACTCCGATGAGTCGGCGGGACAGCTCAGCGACCTCGAAGAGCAGCTTCAGGAACAGGTCTACGGCCAGC
CGGAAGCGATCAAGGCGCTCTCCAGTGCTCTGCGCCGCGCCCGAGTGGGCCTGGGCGGACGCACCCGCGTCTCGGCCAGC
TTCCTGTTCGTCGGCCCCAGCGGAGTCGGCAAGACCCACCTCGCCAAGGCGCTGGCCAAGACCCTCTTTGGCTCCGAGCG
CTCGCTGATCCGGGTGGACATGAGTGAGTTCCAGGAGGCGCACTCCATCTCCAAGCTGATCGGGTCGCCTCCCGGCTATG
TGGGCTTCGAGCAGGGCGGACGCCTGACCGAGGCGGTGCGTCGCCAGCCTTTCTCGGTGATCCTGCTCGACGAGATCGAA
AAGGCGCACCCGGATGTCTACAACACCTTCTTGCAGGTGCTGGACGATGGCCGCCTCACCGACGGGCTGGGCCGCACGGT
GGATTTCCGCCGCACCATCATCATCATGACGAGCAACACGGGCTTTAACGTCAACCCCACCGTGGGCTTCAGCCCGGTCA
CTCCCGACAACAATGCGCCGCTGCGCAACATCTTCACCCCCGAATTCCTCGATCGCCTCGACGATGTGATCCGCTTCCGC
CCGCTGGGCGAGGACGAACTGGTGCGTGTCGCGCAGCAACTGCTGGGCGAGATGCGTGAGGAACTCGCCAGCCGCGAGCT
GAACGTCACCTTCGACCCGGCCATCGCCGCCTGGCTGGTGAGCAAGCTCAAGGCACGTAGCCCCAAGCACGCCGTCGGTT
CCAGCCGGCAGCTGCGCACCCTGCTGCGCGAGGAGATCGAGGACCCGCTGGCCCTTGAGCTGATTGGTAACGCGGGTGAG
GAGCTGCGGGTGGTGCTGGGCCAGGATGGCATCCAGTTCGAGCGCGGCAAGACGGCACCGCCGCAGATTCTGGCGTAG

Upstream 100 bases:

>100_bases
ACTAGAATCGATGCAGCACCTTCGCCCGCTTTTCAACACGCTGCGCTCCGGCGCAACGTGGCCTGCCCAAGCCAAGCCTG
CAGGGCACAGGGGGTAACCC

Downstream 100 bases:

>100_bases
GGCCTGTTCAACCCGGCTGGGCGGAGCGACCTCGTGTCCTCCGCCCGCTGTCTTCCAGGTAGAGTTGCTGCCAAATGCAG
CCGCTCCTCCTGACCCTAAC

Product: ATPase AAA-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MNRYDDRARLVFHYAREEGNRLGHAMVGPEHLLLGLMREGGTAATILSEFGASLDGLRRRVEEIIGRGEGNRLNDAPSIT
PRARRVMELASAEARSLGAQVTSTEHILLGIIREGDGVAFRILQELTKDVDTIRWRVLAQGDGSSGKAAKPVATPFLDEY
GRDLTKQAREGKLDPVIGRSEEIRRVTQILTRRTKNNPVLIGDPGVGKTAIVEGLALAIFEKRTPPNLHGVRLVSLDLSG
VVAGTKYRGEFEERLRQIIEELRNAKVMAFIDELHTLVGAGGAEGTLDAANILKPALSRGEIQVIGATTTGEYHRYIEKD
AALERRFQPVIVLEPSPAETLQILRGLRPRYEEHHGVQIPESALELAVRIGERSLPGRNFPDKAIDLIDEAASRVRLNMS
VGLPVSETEDGEPMVSREDIESVINSMGGIYSDESAGQLSDLEEQLQEQVYGQPEAIKALSSALRRARVGLGGRTRVSAS
FLFVGPSGVGKTHLAKALAKTLFGSERSLIRVDMSEFQEAHSISKLIGSPPGYVGFEQGGRLTEAVRRQPFSVILLDEIE
KAHPDVYNTFLQVLDDGRLTDGLGRTVDFRRTIIIMTSNTGFNVNPTVGFSPVTPDNNAPLRNIFTPEFLDRLDDVIRFR
PLGEDELVRVAQQLLGEMREELASRELNVTFDPAIAAWLVSKLKARSPKHAVGSSRQLRTLLREEIEDPLALELIGNAGE
ELRVVLGQDGIQFERGKTAPPQILA

Sequences:

>Translated_745_residues
MNRYDDRARLVFHYAREEGNRLGHAMVGPEHLLLGLMREGGTAATILSEFGASLDGLRRRVEEIIGRGEGNRLNDAPSIT
PRARRVMELASAEARSLGAQVTSTEHILLGIIREGDGVAFRILQELTKDVDTIRWRVLAQGDGSSGKAAKPVATPFLDEY
GRDLTKQAREGKLDPVIGRSEEIRRVTQILTRRTKNNPVLIGDPGVGKTAIVEGLALAIFEKRTPPNLHGVRLVSLDLSG
VVAGTKYRGEFEERLRQIIEELRNAKVMAFIDELHTLVGAGGAEGTLDAANILKPALSRGEIQVIGATTTGEYHRYIEKD
AALERRFQPVIVLEPSPAETLQILRGLRPRYEEHHGVQIPESALELAVRIGERSLPGRNFPDKAIDLIDEAASRVRLNMS
VGLPVSETEDGEPMVSREDIESVINSMGGIYSDESAGQLSDLEEQLQEQVYGQPEAIKALSSALRRARVGLGGRTRVSAS
FLFVGPSGVGKTHLAKALAKTLFGSERSLIRVDMSEFQEAHSISKLIGSPPGYVGFEQGGRLTEAVRRQPFSVILLDEIE
KAHPDVYNTFLQVLDDGRLTDGLGRTVDFRRTIIIMTSNTGFNVNPTVGFSPVTPDNNAPLRNIFTPEFLDRLDDVIRFR
PLGEDELVRVAQQLLGEMREELASRELNVTFDPAIAAWLVSKLKARSPKHAVGSSRQLRTLLREEIEDPLALELIGNAGE
ELRVVLGQDGIQFERGKTAPPQILA
>Mature_745_residues
MNRYDDRARLVFHYAREEGNRLGHAMVGPEHLLLGLMREGGTAATILSEFGASLDGLRRRVEEIIGRGEGNRLNDAPSIT
PRARRVMELASAEARSLGAQVTSTEHILLGIIREGDGVAFRILQELTKDVDTIRWRVLAQGDGSSGKAAKPVATPFLDEY
GRDLTKQAREGKLDPVIGRSEEIRRVTQILTRRTKNNPVLIGDPGVGKTAIVEGLALAIFEKRTPPNLHGVRLVSLDLSG
VVAGTKYRGEFEERLRQIIEELRNAKVMAFIDELHTLVGAGGAEGTLDAANILKPALSRGEIQVIGATTTGEYHRYIEKD
AALERRFQPVIVLEPSPAETLQILRGLRPRYEEHHGVQIPESALELAVRIGERSLPGRNFPDKAIDLIDEAASRVRLNMS
VGLPVSETEDGEPMVSREDIESVINSMGGIYSDESAGQLSDLEEQLQEQVYGQPEAIKALSSALRRARVGLGGRTRVSAS
FLFVGPSGVGKTHLAKALAKTLFGSERSLIRVDMSEFQEAHSISKLIGSPPGYVGFEQGGRLTEAVRRQPFSVILLDEIE
KAHPDVYNTFLQVLDDGRLTDGLGRTVDFRRTIIIMTSNTGFNVNPTVGFSPVTPDNNAPLRNIFTPEFLDRLDDVIRFR
PLGEDELVRVAQQLLGEMREELASRELNVTFDPAIAAWLVSKLKARSPKHAVGSSRQLRTLLREEIEDPLALELIGNAGE
ELRVVLGQDGIQFERGKTAPPQILA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=291, Percent_Identity=36.7697594501718, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1787109, Length=731, Percent_Identity=38.4404924760602, Blast_Score=464, Evalue=1e-131,
Organism=Escherichia coli, GI1788943, Length=395, Percent_Identity=45.8227848101266, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6323002, Length=328, Percent_Identity=45.4268292682927, Blast_Score=275, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=245, Percent_Identity=55.9183673469388, Blast_Score=271, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 81848; Mature: 81848

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYDDRARLVFHYAREEGNRLGHAMVGPEHLLLGLMREGGTAATILSEFGASLDGLRRR
CCCCCCHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
VEEIIGRGEGNRLNDAPSITPRARRVMELASAEARSLGAQVTSTEHILLGIIREGDGVAF
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHH
RILQELTKDVDTIRWRVLAQGDGSSGKAAKPVATPFLDEYGRDLTKQAREGKLDPVIGRS
HHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHCCCCCCCCCCH
EEIRRVTQILTRRTKNNPVLIGDPGVGKTAIVEGLALAIFEKRTPPNLHGVRLVSLDLSG
HHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
VVAGTKYRGEFEERLRQIIEELRNAKVMAFIDELHTLVGAGGAEGTLDAANILKPALSRG
EEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
EIQVIGATTTGEYHRYIEKDAALERRFQPVIVLEPSPAETLQILRGLRPRYEEHHGVQIP
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCHHHCCCCCCC
ESALELAVRIGERSLPGRNFPDKAIDLIDEAASRVRLNMSVGLPVSETEDGEPMVSREDI
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHHHH
ESVINSMGGIYSDESAGQLSDLEEQLQEQVYGQPEAIKALSSALRRARVGLGGRTRVSAS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
FLFVGPSGVGKTHLAKALAKTLFGSERSLIRVDMSEFQEAHSISKLIGSPPGYVGFEQGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
RLTEAVRRQPFSVILLDEIEKAHPDVYNTFLQVLDDGRLTDGLGRTVDFRRTIIIMTSNT
HHHHHHHHCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEEEEECCC
GFNVNPTVGFSPVTPDNNAPLRNIFTPEFLDRLDDVIRFRPLGEDELVRVAQQLLGEMRE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ELASRELNVTFDPAIAAWLVSKLKARSPKHAVGSSRQLRTLLREEIEDPLALELIGNAGE
HHHCCCCCEEECHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHCCCCEEEHHHCCCCC
ELRVVLGQDGIQFERGKTAPPQILA
EEEEEECCCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MNRYDDRARLVFHYAREEGNRLGHAMVGPEHLLLGLMREGGTAATILSEFGASLDGLRRR
CCCCCCHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
VEEIIGRGEGNRLNDAPSITPRARRVMELASAEARSLGAQVTSTEHILLGIIREGDGVAF
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHH
RILQELTKDVDTIRWRVLAQGDGSSGKAAKPVATPFLDEYGRDLTKQAREGKLDPVIGRS
HHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHHCCCCCCCCCCH
EEIRRVTQILTRRTKNNPVLIGDPGVGKTAIVEGLALAIFEKRTPPNLHGVRLVSLDLSG
HHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
VVAGTKYRGEFEERLRQIIEELRNAKVMAFIDELHTLVGAGGAEGTLDAANILKPALSRG
EEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
EIQVIGATTTGEYHRYIEKDAALERRFQPVIVLEPSPAETLQILRGLRPRYEEHHGVQIP
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCHHHCCCCCCC
ESALELAVRIGERSLPGRNFPDKAIDLIDEAASRVRLNMSVGLPVSETEDGEPMVSREDI
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHHHH
ESVINSMGGIYSDESAGQLSDLEEQLQEQVYGQPEAIKALSSALRRARVGLGGRTRVSAS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
FLFVGPSGVGKTHLAKALAKTLFGSERSLIRVDMSEFQEAHSISKLIGSPPGYVGFEQGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
RLTEAVRRQPFSVILLDEIEKAHPDVYNTFLQVLDDGRLTDGLGRTVDFRRTIIIMTSNT
HHHHHHHHCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEEEEECCC
GFNVNPTVGFSPVTPDNNAPLRNIFTPEFLDRLDDVIRFRPLGEDELVRVAQQLLGEMRE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ELASRELNVTFDPAIAAWLVSKLKARSPKHAVGSSRQLRTLLREEIEDPLALELIGNAGE
HHHCCCCCEEECHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHCCCCEEEHHHCCCCC
ELRVVLGQDGIQFERGKTAPPQILA
EEEEEECCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]