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The map label for this gene is pstI [H]

Identifier: 94985083

GI number: 94985083

Start: 1049083

End: 1050627

Strand: Reverse

Name: pstI [H]

Synonym: Dgeo_0978

Alternate gene names: 94985083

Gene position: 1050627-1049083 (Counterclockwise)

Preceding gene: 94985092

Following gene: 94985082

Centisome position: 42.58

GC content: 67.57

Gene sequence:

>1545_bases
ATGAACTCACGTCTTGCGCTTGGTCTTCTCGGCCTCACGCTCCTGCTCGCGGCCTGCGGACAGCAGGCGAATACGCCGGC
GGACACGGCCCAGGCCAGCACGCCGGATCGCAGCAGCCACACGGCGCCCCTGCTGGGCACGAGCAATCCCGAGGCGATTC
CCGGCCAGTACATCGTGGTGTTCAGCGACGGCGCGCTGGGAGCGAATCTGGGCGCGCAGGATGCCGGAAGCCTGATCCGC
ACGCTCGGACTGGATCCCCAGGGCATCAGCGTGCAGCACATCTACACGCAGGCCCTTAGCGGCTTCGCGGCCAAGCTCAG
CGCGCAGAACCTCGCCAAACTTCAGGCGGACCGGCGGGTCAAGTACATCGAGCAGGACGCGACGGTCCACGCCACCGCCA
CCCAGAGCGGTGCCACCTGGGGCCTGGACCGCATCGACCAACGCAACCTGCCCCTCGATGGCAACTACAGCTACAGCACG
ACGGCCAGCAACGTCACCGCCTACATCATCGACACTGGGATCAACACGGCGCATACGGATTTCGGCGGACGGGCGGTGTG
GGGCACCAACACCACCGGGGACGGCAACAACAGTGACTGCCAGGGGCACGGAACGCACGTGGCGGGCACGGTGGGCAGCA
GCACCTGGGGCGTGGCCAAGGGCGTGAAGCTCGTCGCCGTGAAGGTGCTGGGCTGTGACGGCAGCGGCACAAACTCCGGC
GTGATCGCGGGCGTCAACTGGGCCGTGAGCAACAAGAGCGGTCCCGCGGTGGCGAACATGAGCCTGGGCGGTGGCGTCAG
CCAGGCGCTCGACGACGCGGTGAACAACGCCGCCAGCAAGAATCTGGTGATGGCGGTCGCGGGCGGGAACGACAATGTGG
ACGCCTGCACCAGCAGCCCGGCGCGCGCCGCGAACGCCATCACCGTCGGCGCGACCGACCGGAACGATGCCCGCGCCAGC
TTCAGCAACTATGGCTCTTGCCTCGATCTCTTCGCCCCCGGCGTAAACATCACCAGCACCTGGATCGGCTCCACCACCGC
CACCAACACCATCAGCGGCACCAGCATGGCGACGCCCCACGTGACCGGCGCGGCGGCCCTGATCCTGGCGGCCAACCCCT
CCTACACGACGGCTCAGGTCACCAGCGCTCTGCTGAATAACGCCACGACCGGCAAGGTCACCTCCGCGGGCAGCGGCAGC
CCCAACCGCCTGCTCTACACCGGCAGCGGCAGCACCACGCCCGCTCCCGGTACCTCGACCACCTACAGCGGCTCGGTCAG
TCAGGGCAGCAGCAGCTGGAAGCCCAGCACCAGCGGCTTCAGCTACGCGGGCGGCACCCTCAGGGGCACGCTGAGCGGCC
CCAGCGGAACGGATTTTGACCTCTATCTCCAAAAGTACAACGGCAGCAGCTGGGTGGATGTGGCGGCCAGCGAAGGCAGC
AGCAGCAGCGAGAGCATCAACTATGTGGCGGGCAGCGGCACCTACCGCTGGGAGGTCTACGCCTATAGCGGCAGCGGCAG
CTACACCCTGGTCGAGACGAAGTAG

Upstream 100 bases:

>100_bases
TTGTCTAGATGTTTCAAAAAAGGGCCTTGGAAGGCCCGTGATACACCTTCGCTTGTCGGAAAACCAAACTTTCTCATCAG
CCCAACCCCGGAGGTTCAGG

Downstream 100 bases:

>100_bases
AAGTCACAGAGCAGGAGCGGCGAGGGGCATCCGGCGACGGGTGCCTCCTTGTCGTTTTGGATGCGGTCAGGCAGGAACAA
AGAGCAGAGGCGCGCGCCGA

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Aqualysin-I [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNSRLALGLLGLTLLLAACGQQANTPADTAQASTPDRSSHTAPLLGTSNPEAIPGQYIVVFSDGALGANLGAQDAGSLIR
TLGLDPQGISVQHIYTQALSGFAAKLSAQNLAKLQADRRVKYIEQDATVHATATQSGATWGLDRIDQRNLPLDGNYSYST
TASNVTAYIIDTGINTAHTDFGGRAVWGTNTTGDGNNSDCQGHGTHVAGTVGSSTWGVAKGVKLVAVKVLGCDGSGTNSG
VIAGVNWAVSNKSGPAVANMSLGGGVSQALDDAVNNAASKNLVMAVAGGNDNVDACTSSPARAANAITVGATDRNDARAS
FSNYGSCLDLFAPGVNITSTWIGSTTATNTISGTSMATPHVTGAAALILAANPSYTTAQVTSALLNNATTGKVTSAGSGS
PNRLLYTGSGSTTPAPGTSTTYSGSVSQGSSSWKPSTSGFSYAGGTLRGTLSGPSGTDFDLYLQKYNGSSWVDVAASEGS
SSSESINYVAGSGTYRWEVYAYSGSGSYTLVETK

Sequences:

>Translated_514_residues
MNSRLALGLLGLTLLLAACGQQANTPADTAQASTPDRSSHTAPLLGTSNPEAIPGQYIVVFSDGALGANLGAQDAGSLIR
TLGLDPQGISVQHIYTQALSGFAAKLSAQNLAKLQADRRVKYIEQDATVHATATQSGATWGLDRIDQRNLPLDGNYSYST
TASNVTAYIIDTGINTAHTDFGGRAVWGTNTTGDGNNSDCQGHGTHVAGTVGSSTWGVAKGVKLVAVKVLGCDGSGTNSG
VIAGVNWAVSNKSGPAVANMSLGGGVSQALDDAVNNAASKNLVMAVAGGNDNVDACTSSPARAANAITVGATDRNDARAS
FSNYGSCLDLFAPGVNITSTWIGSTTATNTISGTSMATPHVTGAAALILAANPSYTTAQVTSALLNNATTGKVTSAGSGS
PNRLLYTGSGSTTPAPGTSTTYSGSVSQGSSSWKPSTSGFSYAGGTLRGTLSGPSGTDFDLYLQKYNGSSWVDVAASEGS
SSSESINYVAGSGTYRWEVYAYSGSGSYTLVETK
>Mature_514_residues
MNSRLALGLLGLTLLLAACGQQANTPADTAQASTPDRSSHTAPLLGTSNPEAIPGQYIVVFSDGALGANLGAQDAGSLIR
TLGLDPQGISVQHIYTQALSGFAAKLSAQNLAKLQADRRVKYIEQDATVHATATQSGATWGLDRIDQRNLPLDGNYSYST
TASNVTAYIIDTGINTAHTDFGGRAVWGTNTTGDGNNSDCQGHGTHVAGTVGSSTWGVAKGVKLVAVKVLGCDGSGTNSG
VIAGVNWAVSNKSGPAVANMSLGGGVSQALDDAVNNAASKNLVMAVAGGNDNVDACTSSPARAANAITVGATDRNDARAS
FSNYGSCLDLFAPGVNITSTWIGSTTATNTISGTSMATPHVTGAAALILAANPSYTTAQVTSALLNNATTGKVTSAGSGS
PNRLLYTGSGSTTPAPGTSTTYSGSVSQGSSSWKPSTSGFSYAGGTLRGTLSGPSGTDFDLYLQKYNGSSWVDVAASEGS
SSSESINYVAGSGTYRWEVYAYSGSGSYTLVETK

Specific function: Aqualysin I is a thermophilic alkaline serine protease [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI31317307, Length=383, Percent_Identity=32.6370757180157, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4506775, Length=337, Percent_Identity=24.9258160237389, Blast_Score=73, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320775, Length=440, Percent_Identity=39.7727272727273, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324576, Length=338, Percent_Identity=40.8284023668639, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319893, Length=312, Percent_Identity=39.7435897435897, Blast_Score=186, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.111 [H]

Molecular weight: Translated: 51859; Mature: 51859

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER ; PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRLALGLLGLTLLLAACGQQANTPADTAQASTPDRSSHTAPLLGTSNPEAIPGQYIVV
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEE
FSDGALGANLGAQDAGSLIRTLGLDPQGISVQHIYTQALSGFAAKLSAQNLAKLQADRRV
ECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
KYIEQDATVHATATQSGATWGLDRIDQRNLPLDGNYSYSTTASNVTAYIIDTGINTAHTD
EEEECCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCC
FGGRAVWGTNTTGDGNNSDCQGHGTHVAGTVGSSTWGVAKGVKLVAVKVLGCDGSGTNSG
CCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCEEEEEEEEECCCCCCCCC
VIAGVNWAVSNKSGPAVANMSLGGGVSQALDDAVNNAASKNLVMAVAGGNDNVDACTSSP
EEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
ARAANAITVGATDRNDARASFSNYGSCLDLFAPGVNITSTWIGSTTATNTISGTSMATPH
CCCCCEEEECCCCCCCCHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCC
VTGAAALILAANPSYTTAQVTSALLNNATTGKVTSAGSGSPNRLLYTGSGSTTPAPGTST
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCC
TYSGSVSQGSSSWKPSTSGFSYAGGTLRGTLSGPSGTDFDLYLQKYNGSSWVDVAASEGS
EECCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEECCCC
SSSESINYVAGSGTYRWEVYAYSGSGSYTLVETK
CCCCCEEEEECCCEEEEEEEEECCCCCEEEEEEC
>Mature Secondary Structure
MNSRLALGLLGLTLLLAACGQQANTPADTAQASTPDRSSHTAPLLGTSNPEAIPGQYIVV
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEE
FSDGALGANLGAQDAGSLIRTLGLDPQGISVQHIYTQALSGFAAKLSAQNLAKLQADRRV
ECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
KYIEQDATVHATATQSGATWGLDRIDQRNLPLDGNYSYSTTASNVTAYIIDTGINTAHTD
EEEECCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCC
FGGRAVWGTNTTGDGNNSDCQGHGTHVAGTVGSSTWGVAKGVKLVAVKVLGCDGSGTNSG
CCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCEEEEEEEEECCCCCCCCC
VIAGVNWAVSNKSGPAVANMSLGGGVSQALDDAVNNAASKNLVMAVAGGNDNVDACTSSP
EEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
ARAANAITVGATDRNDARASFSNYGSCLDLFAPGVNITSTWIGSTTATNTISGTSMATPH
CCCCCEEEECCCCCCCCHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCC
VTGAAALILAANPSYTTAQVTSALLNNATTGKVTSAGSGSPNRLLYTGSGSTTPAPGTST
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCC
TYSGSVSQGSSSWKPSTSGFSYAGGTLRGTLSGPSGTDFDLYLQKYNGSSWVDVAASEGS
EECCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEECCCC
SSSESINYVAGSGTYRWEVYAYSGSGSYTLVETK
CCCCCEEEEECCCEEEEEEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2182621; 3286255; 3162211 [H]