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The map label for this gene is metG [H]

Identifier: 94984831

GI number: 94984831

Start: 781714

End: 783726

Strand: Reverse

Name: metG [H]

Synonym: Dgeo_0724

Alternate gene names: 94984831

Gene position: 783726-781714 (Counterclockwise)

Preceding gene: 94984834

Following gene: 94984830

Centisome position: 31.77

GC content: 65.42

Gene sequence:

>2013_bases
ATGACGAGACAGCCTGAGTCCACACAGAACGAGTTTTTTATTACGACCGCCATTGACTACGCCAACGGCGCGCCCCACAT
CGGGCACGTGTACGAGAAGATCCTGGCGGACGCCCTGGCCCGCTACCACCGCCTTGCCGGGTACGACGTGACCTTTGTGA
CCGGTACCGACGAACACGGCGAGAAGATCGCCAAGGCTGCCGCCAAGGCCGGGCAGACGCCCCAGACCTTCGTGGACGAC
CTTTCCCTGCGGGCCTTCAAGGGCCTATGGGACCGCCTGGAGATCAGTTACGACGACTTTATCCGCACCACCGAAGGCCG
CCACAAACGCTTCGTGGCAGAGATTCTCCAGCGCGTGTACGACGCGGGTGACATCTACTTCGCGGAGTACGAGGGCCTCT
ACTCGGTCGGGGCCGAACGCTACGTGACCGAAAAGGAATTGGTGGAGGGCCCCGACGGCGTGCGCCGCTATCCCGGCGAC
AAGGACCCGCCCGAGCTGCGGCGCGAGGCCAACTACTTCTTCCGGATGGAGAAATATCAGGACTGGCTGCGCGCGCACAT
CGAGCAGAACCCCGACTTCATCCAGCCTGCCGGATACCGGAATGAGGTGCTCGAGATGCTGCGCGAGCCTATCGGGGACC
TCTCCATCAGCCGCCCGAAAAGCCGTGTGCCGTGGGGCATCGAGCTGCCTTGGGACCCCGACCACGTGACCTACGTGTGG
TTCGACGCGCTGCTGAACTACGTCTCGGCGCCGGTCAGCCGCGGGCTTGGCGAGGAGACCATCGGCAAGGCGTGGCATGT
GATTGGCAAGGACATCCTCAAGCCGCACGCGGTCTTCTGGCCGACCATGCTGCGGGCGGCGGGATTGCCGATCTACCGCA
AGCTGGTCGTCCACAGCCACATCCTCGCGGAGGACGGGCGCAAGATGGGCAAGTCACTCGGCAACGCCATCGACCCCGAA
CAACTCGTGCGCGAGTACCCGGTGGACGCGATCCGCTACACACTGCTGCGTGAGGCCACGCTGAGTGCCGACAGTCCCTA
CGGCGAGGGGATTCTGGTCTCGCGCCTGAATTCCGACTTGGCCAACGACCTGGGCAACCTGCTCTCCCGTACGGTGAGCA
TGATCCAGAAGTACCGGGGCGGCGTTCTGCCGCAAGCGCTGGAGGTAGGAGAGCGTGAGCGTGAGGTCGAGGCGAAGGCG
CTGGCCCTTCCCAGCGAGGTGATGACGCTGGTCCGCGATCTCAAGGTGAACATGGCGATTGAGACCGCAATGAACTTTGT
GCGCGACCTCAACCGCTACATCGCAGAGAGTCAGCCGTGGAACCTCGCCAAATCGGAGGAGACGGCCCGCCGCCTGGACA
CGGTGCTGTATACCGCCGCCGAGGGCCTGCGCGTGGCCAGCGTGGCGCTGGAAGCCGTGATTCCCGGCAAGGCCCGCGAA
CTGCGCGCGCAGCTTGGCTTGGGCGGGCAGTCCTACGCGCTCAGGGGAGCCTGGGGCCTCACGCCCGCCGGAACGCGGGT
GGTGGGCGGTCCGGTCCTCTTCCCCAAACCAGAACTGCCCAAACCCGAGGCCCCAGAAGCGGCCGCCACTCCACCCACCC
CCAGAAAAGAGAAGACAACCGTGACCCCTCCCCGGAACGCTCAGGCCCCTGCCCCCACCCCCACCGCCACGCCCGCCGCC
CCCGAAGCGCTCATCTCCATCGACGACTTCGCCCGGGTGGACCTGCGGATTGCGGAAGTTGTGGCCGCCGAGGCCGTCGA
GAAGGCCGATAAGCTCCTCAAACTCACTGTGAAGATGGGTGACGAGACACGCACGGTCGTGAGCGGCATCCGCAAGTGGT
TTGCGCCCGAAGATCTGGTGGGCCGCAAGGTGATCTTGGTGGCGAACCTCAAGCCCGCGCGGCTGCGCGGCATCGAGTCG
CAGGGCATGATTCTGGCCGCCGAGGATGACGCCGGGAATCTCGACTTGGTGGGCACGCGGCTGGACCTGCCGAGCGGAAC
GAAAGTGCGGTAG

Upstream 100 bases:

>100_bases
CTTGTCTCAGACACTTTCCTGCGGCGCGTGATTTTTTCCGTATCTTTGGGAAGTTGCCTGGCCCTCCTCTCATCAGGGAC
CGGCCAGGCGGGGAGCGGCC

Downstream 100 bases:

>100_bases
ACCAAGGATCCGCTGCTCACCTCGTGTGAGGGCGCTGTGGAGGGCAGACGTCAGCGGCCTGCCCTCCCGCTACACTACCG
CCATGCCGTTTGTGGTTGTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTRQPESTQNEFFITTAIDYANGAPHIGHVYEKILADALARYHRLAGYDVTFVTGTDEHGEKIAKAAAKAGQTPQTFVDD
LSLRAFKGLWDRLEISYDDFIRTTEGRHKRFVAEILQRVYDAGDIYFAEYEGLYSVGAERYVTEKELVEGPDGVRRYPGD
KDPPELRREANYFFRMEKYQDWLRAHIEQNPDFIQPAGYRNEVLEMLREPIGDLSISRPKSRVPWGIELPWDPDHVTYVW
FDALLNYVSAPVSRGLGEETIGKAWHVIGKDILKPHAVFWPTMLRAAGLPIYRKLVVHSHILAEDGRKMGKSLGNAIDPE
QLVREYPVDAIRYTLLREATLSADSPYGEGILVSRLNSDLANDLGNLLSRTVSMIQKYRGGVLPQALEVGEREREVEAKA
LALPSEVMTLVRDLKVNMAIETAMNFVRDLNRYIAESQPWNLAKSEETARRLDTVLYTAAEGLRVASVALEAVIPGKARE
LRAQLGLGGQSYALRGAWGLTPAGTRVVGGPVLFPKPELPKPEAPEAAATPPTPRKEKTTVTPPRNAQAPAPTPTATPAA
PEALISIDDFARVDLRIAEVVAAEAVEKADKLLKLTVKMGDETRTVVSGIRKWFAPEDLVGRKVILVANLKPARLRGIES
QGMILAAEDDAGNLDLVGTRLDLPSGTKVR

Sequences:

>Translated_670_residues
MTRQPESTQNEFFITTAIDYANGAPHIGHVYEKILADALARYHRLAGYDVTFVTGTDEHGEKIAKAAAKAGQTPQTFVDD
LSLRAFKGLWDRLEISYDDFIRTTEGRHKRFVAEILQRVYDAGDIYFAEYEGLYSVGAERYVTEKELVEGPDGVRRYPGD
KDPPELRREANYFFRMEKYQDWLRAHIEQNPDFIQPAGYRNEVLEMLREPIGDLSISRPKSRVPWGIELPWDPDHVTYVW
FDALLNYVSAPVSRGLGEETIGKAWHVIGKDILKPHAVFWPTMLRAAGLPIYRKLVVHSHILAEDGRKMGKSLGNAIDPE
QLVREYPVDAIRYTLLREATLSADSPYGEGILVSRLNSDLANDLGNLLSRTVSMIQKYRGGVLPQALEVGEREREVEAKA
LALPSEVMTLVRDLKVNMAIETAMNFVRDLNRYIAESQPWNLAKSEETARRLDTVLYTAAEGLRVASVALEAVIPGKARE
LRAQLGLGGQSYALRGAWGLTPAGTRVVGGPVLFPKPELPKPEAPEAAATPPTPRKEKTTVTPPRNAQAPAPTPTATPAA
PEALISIDDFARVDLRIAEVVAAEAVEKADKLLKLTVKMGDETRTVVSGIRKWFAPEDLVGRKVILVANLKPARLRGIES
QGMILAAEDDAGNLDLVGTRLDLPSGTKVR
>Mature_669_residues
TRQPESTQNEFFITTAIDYANGAPHIGHVYEKILADALARYHRLAGYDVTFVTGTDEHGEKIAKAAAKAGQTPQTFVDDL
SLRAFKGLWDRLEISYDDFIRTTEGRHKRFVAEILQRVYDAGDIYFAEYEGLYSVGAERYVTEKELVEGPDGVRRYPGDK
DPPELRREANYFFRMEKYQDWLRAHIEQNPDFIQPAGYRNEVLEMLREPIGDLSISRPKSRVPWGIELPWDPDHVTYVWF
DALLNYVSAPVSRGLGEETIGKAWHVIGKDILKPHAVFWPTMLRAAGLPIYRKLVVHSHILAEDGRKMGKSLGNAIDPEQ
LVREYPVDAIRYTLLREATLSADSPYGEGILVSRLNSDLANDLGNLLSRTVSMIQKYRGGVLPQALEVGEREREVEAKAL
ALPSEVMTLVRDLKVNMAIETAMNFVRDLNRYIAESQPWNLAKSEETARRLDTVLYTAAEGLRVASVALEAVIPGKAREL
RAQLGLGGQSYALRGAWGLTPAGTRVVGGPVLFPKPELPKPEAPEAAATPPTPRKEKTTVTPPRNAQAPAPTPTATPAAP
EALISIDDFARVDLRIAEVVAAEAVEKADKLLKLTVKMGDETRTVVSGIRKWFAPEDLVGRKVILVANLKPARLRGIESQ
GMILAAEDDAGNLDLVGTRLDLPSGTKVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=36.8627450980392, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI14043022, Length=596, Percent_Identity=25, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI4507947, Length=137, Percent_Identity=33.5766423357664, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788432, Length=688, Percent_Identity=28.4883720930233, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=33.6448598130841, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI212645096, Length=492, Percent_Identity=35.5691056910569, Blast_Score=268, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI71992781, Length=385, Percent_Identity=33.2467532467532, Blast_Score=183, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17540794, Length=534, Percent_Identity=26.2172284644195, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=530, Percent_Identity=32.6415094339623, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=422, Percent_Identity=25.1184834123223, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=36.6733466933868, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24655311, Length=536, Percent_Identity=25, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74341; Mature: 74209

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQPESTQNEFFITTAIDYANGAPHIGHVYEKILADALARYHRLAGYDVTFVTGTDEHG
CCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
EKIAKAAAKAGQTPQTFVDDLSLRAFKGLWDRLEISYDDFIRTTEGRHKRFVAEILQRVY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHH
DAGDIYFAEYEGLYSVGAERYVTEKELVEGPDGVRRYPGDKDPPELRREANYFFRMEKYQ
CCCCEEEEECCCHHHHCHHHHCCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DWLRAHIEQNPDFIQPAGYRNEVLEMLREPIGDLSISRPKSRVPWGIELPWDPDHVTYVW
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHHHH
FDALLNYVSAPVSRGLGEETIGKAWHVIGKDILKPHAVFWPTMLRAAGLPIYRKLVVHSH
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHH
ILAEDGRKMGKSLGNAIDPEQLVREYPVDAIRYTLLREATLSADSPYGEGILVSRLNSDL
HHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
ANDLGNLLSRTVSMIQKYRGGVLPQALEVGEREREVEAKALALPSEVMTLVRDLKVNMAI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ETAMNFVRDLNRYIAESQPWNLAKSEETARRLDTVLYTAAEGLRVASVALEAVIPGKARE
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
LRAQLGLGGQSYALRGAWGLTPAGTRVVGGPVLFPKPELPKPEAPEAAATPPTPRKEKTT
HHHHHCCCCCCEEEECCCCCCCCCCEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
VTPPRNAQAPAPTPTATPAAPEALISIDDFARVDLRIAEVVAAEAVEKADKLLKLTVKMG
CCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DETRTVVSGIRKWFAPEDLVGRKVILVANLKPARLRGIESQGMILAAEDDAGNLDLVGTR
CCHHHHHHHHHHHCCCHHHCCCEEEEEECCCHHHHCCCCCCCEEEEECCCCCCEEEEEEE
LDLPSGTKVR
ECCCCCCCCC
>Mature Secondary Structure 
TRQPESTQNEFFITTAIDYANGAPHIGHVYEKILADALARYHRLAGYDVTFVTGTDEHG
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH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ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]