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The map label for this gene is metZ [H]

Identifier: 94984625

GI number: 94984625

Start: 545766

End: 547097

Strand: Reverse

Name: metZ [H]

Synonym: Dgeo_0518

Alternate gene names: 94984625

Gene position: 547097-545766 (Counterclockwise)

Preceding gene: 94984626

Following gene: 94984624

Centisome position: 22.17

GC content: 68.24

Gene sequence:

>1332_bases
ATGGCTTCCAATACGCTGCACTTCGAGACGCTCCAGGTCCACGCCGGACAGCATCCCGACCCTGCGACCGGTGCCCAGGC
CGTGCCGATCTACGCCACCAACGCCTATGTCTTTGAGTCGCCGGAACACGCCGCCGACCTGTTTGGCCTGCGGGCCTTCG
GCAACATCTACAGCCGGATCATGAATCCCACCAACGCCGTGCTGGAGGAACGCATTGCGGCGCTAGAGGGCGGCGTAGGA
GCGCTGGCGGTGGCGAGCGGGCACGCCGCGCAGTTCTTAGCCATCACCACTGTCGCGCAGGCGGGGGACAACATCGTCTC
CACGCCCAACCTCTACGGTGGTACGGTCAACCAGTTCCGCGTTACCCTGCGGCGGCTGGGCATCGAGGTCCGCTTTACCA
GCAAGGACGAGCGCCCGGAGGAATTCGCGGCGCTGATCGACGACCGCACGCGCGCCGTGTATCTGGAAACACTCGGCAAC
CCGGCGCTGAATGTCCCCGATTTTGAGGGCATCGCGGAGGTGGCCCACGCGCGGGGGGTGGCCGTGTTCGTGGACAACAC
CTTCGGGGCGGGCGGGTACTACTGCCAGCCCCTCCGCCACGGCGCGGACGTGGTGCTGCATTCGGCAAGCAAGTGGATCG
GTGGGCACGGCAACGGCATCGGTGGTCTTCTCGTGGACGGCGGAACCTTTGACTGGGGCAATGGCCGCTATCCCCTTCTC
ACCGAACCCAGCCCCTCCTACCACGGCCTGAGCTTCTGGGAGGCGTTTGGCGAGGGGAACGCGCTGGGCCTGCCCAACAT
CGCCTTTATCACCCGCGCCCGCACTGAGGGGCTGCGCGACCTGGGGCCAACGCTCGCGCCGCAGCAGGCCTGGCAGTTCC
TGCAAGGGGTGGAAACCCTCTCGCTGCGCGCCGAGCGGCACGCGCAAAACGCGCTCGCGCTGGCCTCCTGGCTAAGTGGC
CACCCGGACGTGTCACGCGTCACCTATCCGGGCCTGAGCAACCACCCGCACTACGACCGCGCCCAGACGTATCTGCCGCG
CGGGGCGGGGGCCGTGCTGACCTTTGAGCTGCGCGGGGGACGGGCGGCGGGCGAGGCATTTATTGGCGCGGTGCGGCTCG
CACAACATGTCGCCAATGTGGGCGACACCCGCACGCTGGTGATTCACCCCGCCAGCACCACCCACTCCCAGCTGGACGAG
GCGGCGCAGGCGGCCGCGGGCGTGACGCCGGGACTGGTGCGCGTGTCGGTGGGGATCGAGCACATTGACGACATCCGCGA
GGACTTTGCGCAGGCACTGGCCACCGCGCTGGTGGACGCGGAGGGCGCATGA

Upstream 100 bases:

>100_bases
GAATTCTGCCCCGCCCCTTCTCGATGCCCGAGAGGGGGCGCCTCCTTTGCGCGGCTCCCCCACAGCCCCAATAACTTTTT
TCTGTGAGGAGGATCGCCGC

Downstream 100 bases:

>100_bases
CCGCTTTGATCTCCCAGCCGGACCTGCTTCCCCCGCCGGCGCCGGAGCGATGTCCGCCGCAACAGACGGCGCGGCTGTTC
CGCGAGACACCGCTGCTCCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MASNTLHFETLQVHAGQHPDPATGAQAVPIYATNAYVFESPEHAADLFGLRAFGNIYSRIMNPTNAVLEERIAALEGGVG
ALAVASGHAAQFLAITTVAQAGDNIVSTPNLYGGTVNQFRVTLRRLGIEVRFTSKDERPEEFAALIDDRTRAVYLETLGN
PALNVPDFEGIAEVAHARGVAVFVDNTFGAGGYYCQPLRHGADVVLHSASKWIGGHGNGIGGLLVDGGTFDWGNGRYPLL
TEPSPSYHGLSFWEAFGEGNALGLPNIAFITRARTEGLRDLGPTLAPQQAWQFLQGVETLSLRAERHAQNALALASWLSG
HPDVSRVTYPGLSNHPHYDRAQTYLPRGAGAVLTFELRGGRAAGEAFIGAVRLAQHVANVGDTRTLVIHPASTTHSQLDE
AAQAAAGVTPGLVRVSVGIEHIDDIREDFAQALATALVDAEGA

Sequences:

>Translated_443_residues
MASNTLHFETLQVHAGQHPDPATGAQAVPIYATNAYVFESPEHAADLFGLRAFGNIYSRIMNPTNAVLEERIAALEGGVG
ALAVASGHAAQFLAITTVAQAGDNIVSTPNLYGGTVNQFRVTLRRLGIEVRFTSKDERPEEFAALIDDRTRAVYLETLGN
PALNVPDFEGIAEVAHARGVAVFVDNTFGAGGYYCQPLRHGADVVLHSASKWIGGHGNGIGGLLVDGGTFDWGNGRYPLL
TEPSPSYHGLSFWEAFGEGNALGLPNIAFITRARTEGLRDLGPTLAPQQAWQFLQGVETLSLRAERHAQNALALASWLSG
HPDVSRVTYPGLSNHPHYDRAQTYLPRGAGAVLTFELRGGRAAGEAFIGAVRLAQHVANVGDTRTLVIHPASTTHSQLDE
AAQAAAGVTPGLVRVSVGIEHIDDIREDFAQALATALVDAEGA
>Mature_442_residues
ASNTLHFETLQVHAGQHPDPATGAQAVPIYATNAYVFESPEHAADLFGLRAFGNIYSRIMNPTNAVLEERIAALEGGVGA
LAVASGHAAQFLAITTVAQAGDNIVSTPNLYGGTVNQFRVTLRRLGIEVRFTSKDERPEEFAALIDDRTRAVYLETLGNP
ALNVPDFEGIAEVAHARGVAVFVDNTFGAGGYYCQPLRHGADVVLHSASKWIGGHGNGIGGLLVDGGTFDWGNGRYPLLT
EPSPSYHGLSFWEAFGEGNALGLPNIAFITRARTEGLRDLGPTLAPQQAWQFLQGVETLSLRAERHAQNALALASWLSGH
PDVSRVTYPGLSNHPHYDRAQTYLPRGAGAVLTFELRGGRAAGEAFIGAVRLAQHVANVGDTRTLVIHPASTTHSQLDEA
AQAAAGVTPGLVRVSVGIEHIDDIREDFAQALATALVDAEGA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=434, Percent_Identity=30.8755760368664, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI299473759, Length=434, Percent_Identity=27.6497695852535, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI25453487, Length=433, Percent_Identity=27.0207852193995, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1790375, Length=424, Percent_Identity=29.7169811320755, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1789383, Length=442, Percent_Identity=24.6606334841629, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71980708, Length=438, Percent_Identity=31.0502283105023, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=435, Percent_Identity=29.4252873563218, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17538055, Length=438, Percent_Identity=29.2237442922374, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=445, Percent_Identity=54.1573033707865, Blast_Score=437, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319307, Length=436, Percent_Identity=32.7981651376147, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=459, Percent_Identity=25.2723311546841, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=363, Percent_Identity=25.068870523416, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321904, Length=386, Percent_Identity=24.0932642487047, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922554, Length=436, Percent_Identity=30.7339449541284, Blast_Score=182, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 47015; Mature: 46884

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNTLHFETLQVHAGQHPDPATGAQAVPIYATNAYVFESPEHAADLFGLRAFGNIYSRI
CCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECEEEECCCHHHHHHHHHHHHHHHHHHH
MNPTNAVLEERIAALEGGVGALAVASGHAAQFLAITTVAQAGDNIVSTPNLYGGTVNQFR
CCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCCHHHHH
VTLRRLGIEVRFTSKDERPEEFAALIDDRTRAVYLETLGNPALNVPDFEGIAEVAHARGV
HHHHHHCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCE
AVFVDNTFGAGGYYCQPLRHGADVVLHSASKWIGGHGNGIGGLLVDGGTFDWGNGRYPLL
EEEEECCCCCCCEEECHHHCCCCEEEECHHHHCCCCCCCCCEEEECCCEEECCCCCCCEE
TEPSPSYHGLSFWEAFGEGNALGLPNIAFITRARTEGLRDLGPTLAPQQAWQFLQGVETL
CCCCCCCCCHHHHHHHCCCCCCCCCCHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SLRAERHAQNALALASWLSGHPDVSRVTYPGLSNHPHYDRAQTYLPRGAGAVLTFELRGG
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCEEEEEEECCC
RAAGEAFIGAVRLAQHVANVGDTRTLVIHPASTTHSQLDEAAQAAAGVTPGLVRVSVGIE
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHH
HIDDIREDFAQALATALVDAEGA
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASNTLHFETLQVHAGQHPDPATGAQAVPIYATNAYVFESPEHAADLFGLRAFGNIYSRI
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECEEEECCCHHHHHHHHHHHHHHHHHHH
MNPTNAVLEERIAALEGGVGALAVASGHAAQFLAITTVAQAGDNIVSTPNLYGGTVNQFR
CCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCCHHHHH
VTLRRLGIEVRFTSKDERPEEFAALIDDRTRAVYLETLGNPALNVPDFEGIAEVAHARGV
HHHHHHCEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCE
AVFVDNTFGAGGYYCQPLRHGADVVLHSASKWIGGHGNGIGGLLVDGGTFDWGNGRYPLL
EEEEECCCCCCCEEECHHHCCCCEEEECHHHHCCCCCCCCCEEEECCCEEECCCCCCCEE
TEPSPSYHGLSFWEAFGEGNALGLPNIAFITRARTEGLRDLGPTLAPQQAWQFLQGVETL
CCCCCCCCCHHHHHHHCCCCCCCCCCHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SLRAERHAQNALALASWLSGHPDVSRVTYPGLSNHPHYDRAQTYLPRGAGAVLTFELRGG
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCEEEEEEECCC
RAAGEAFIGAVRLAQHVANVGDTRTLVIHPASTTHSQLDEAAQAAAGVTPGLVRVSVGIE
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHH
HIDDIREDFAQALATALVDAEGA
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]