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The map label for this gene is yfhM [H]

Identifier: 94984527

GI number: 94984527

Start: 427146

End: 427958

Strand: Reverse

Name: yfhM [H]

Synonym: Dgeo_0419

Alternate gene names: 94984527

Gene position: 427958-427146 (Counterclockwise)

Preceding gene: 94984528

Following gene: 94984521

Centisome position: 17.35

GC content: 68.02

Gene sequence:

>813_bases
ATGCCAGAGCTGACCGAACATCAGGTCGTCGTGAACGGCGTGCGCCTGCATTGCGTTGCTGCGGGACCGGAAGACGGGCC
GCCCGTGCTGCTGCTACACGGATTCCCAGAGTTCTGGCGGGCCTGGGAGCGTCAGATCGGACCGCTGGCGCGCGCAGGCT
TCCGGGTGGTTGTACCGGACCTGCGCGGCTACAACCTCAGCGAGAAGCCGCCGGGGGTGGCGGCATACCGGGTCAGCACC
TTGCAGAAAGACGTAGCCGCACTGATCCATGCACTGGGGTATCGGCGCTCACACGTCGTCGGGCACGACTGGGGCGGCAT
CATCGCCTGGGCGCTGGCCATCCGGCAGCCGGAAGTGGTGGACCGGCTGGTGATCCTCAACGCTCCGCATCCCGCCGCCG
CCCGCCGGATGCTGCGGCTGCCCCGGCAGTGGCTGCGGTCCTGGTACATCTTCTTCTTCCAGCTCCCATGGCTGCCCGAA
CACTTTCTGCTGCGCTCGGGCCGGAAGATGTTGCGGGGCACGAACCCGGCGGCCTACACCCCGCAGGACCGCAAACTGTA
CCAGCGGGCATGGGAACAGCCCGGCGCCGCCATCGCCATGATCAACTACTACCGCGCCCTGCCGCGCTTCGGCAACGTGA
AGGGCAACGAGGTGCGGGTGCCCACGCTGGTGCTGTGGGGCGAACGGGACATGGTGCTGCGGGCCCTCCAGACGGTCGGG
ACCACCGGCCTAGAATCGGGCAAGGAATCCGCCGAATTCATCGAACCCGAACCGGTGTGGGTGCCCCTCCCGACGCGGGC
GGAAGGGCGTTAG

Upstream 100 bases:

>100_bases
TGATCCCGGCGCTCAAACTCACGCCACGCGGGCTGCTGGATGTGGGGGCCTGGCAGCTTCTACCACGCGAAACGGTGCGT
GTGGGCGCGGAAGGTTGACC

Downstream 100 bases:

>100_bases
CGCTCCCCTGCCGGGTGCGGCGCCTCCTCCGGTGTGAGGCCCAGCCCAGCCAGCACCCGCTCGCGGCTCCAGCCGGTGAG
GGCAGCCAGTTCCTCCAGCA

Product: alpha/beta hydrolase fold

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPELTEHQVVVNGVRLHCVAAGPEDGPPVLLLHGFPEFWRAWERQIGPLARAGFRVVVPDLRGYNLSEKPPGVAAYRVST
LQKDVAALIHALGYRRSHVVGHDWGGIIAWALAIRQPEVVDRLVILNAPHPAAARRMLRLPRQWLRSWYIFFFQLPWLPE
HFLLRSGRKMLRGTNPAAYTPQDRKLYQRAWEQPGAAIAMINYYRALPRFGNVKGNEVRVPTLVLWGERDMVLRALQTVG
TTGLESGKESAEFIEPEPVWVPLPTRAEGR

Sequences:

>Translated_270_residues
MPELTEHQVVVNGVRLHCVAAGPEDGPPVLLLHGFPEFWRAWERQIGPLARAGFRVVVPDLRGYNLSEKPPGVAAYRVST
LQKDVAALIHALGYRRSHVVGHDWGGIIAWALAIRQPEVVDRLVILNAPHPAAARRMLRLPRQWLRSWYIFFFQLPWLPE
HFLLRSGRKMLRGTNPAAYTPQDRKLYQRAWEQPGAAIAMINYYRALPRFGNVKGNEVRVPTLVLWGERDMVLRALQTVG
TTGLESGKESAEFIEPEPVWVPLPTRAEGR
>Mature_269_residues
PELTEHQVVVNGVRLHCVAAGPEDGPPVLLLHGFPEFWRAWERQIGPLARAGFRVVVPDLRGYNLSEKPPGVAAYRVSTL
QKDVAALIHALGYRRSHVVGHDWGGIIAWALAIRQPEVVDRLVILNAPHPAAARRMLRLPRQWLRSWYIFFFQLPWLPEH
FLLRSGRKMLRGTNPAAYTPQDRKLYQRAWEQPGAAIAMINYYRALPRFGNVKGNEVRVPTLVLWGERDMVLRALQTVGT
TGLESGKESAEFIEPEPVWVPLPTRAEGR

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=248, Percent_Identity=38.7096774193548, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI218777840, Length=243, Percent_Identity=39.0946502057613, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI13376164, Length=243, Percent_Identity=39.0946502057613, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI27597073, Length=257, Percent_Identity=29.1828793774319, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI18777795, Length=109, Percent_Identity=34.8623853211009, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554012, Length=266, Percent_Identity=34.9624060150376, Blast_Score=166, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17562298, Length=241, Percent_Identity=34.8547717842324, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30619; Mature: 30488

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELTEHQVVVNGVRLHCVAAGPEDGPPVLLLHGFPEFWRAWERQIGPLARAGFRVVVPD
CCCCCCCCEEEECEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHCHHHHCCCEEEECC
LRGYNLSEKPPGVAAYRVSTLQKDVAALIHALGYRRSHVVGHDWGGIIAWALAIRQPEVV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHH
DRLVILNAPHPAAARRMLRLPRQWLRSWYIFFFQLPWLPEHFLLRSGRKMLRGTNPAAYT
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHCCCCCCCCC
PQDRKLYQRAWEQPGAAIAMINYYRALPRFGNVKGNEVRVPTLVLWGERDMVLRALQTVG
CHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHC
TTGLESGKESAEFIEPEPVWVPLPTRAEGR
CCCCCCCCCHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
PELTEHQVVVNGVRLHCVAAGPEDGPPVLLLHGFPEFWRAWERQIGPLARAGFRVVVPD
CCCCCCCEEEECEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHCHHHHCCCEEEECC
LRGYNLSEKPPGVAAYRVSTLQKDVAALIHALGYRRSHVVGHDWGGIIAWALAIRQPEVV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHH
DRLVILNAPHPAAARRMLRLPRQWLRSWYIFFFQLPWLPEHFLLRSGRKMLRGTNPAAYT
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHCCCCCCCCC
PQDRKLYQRAWEQPGAAIAMINYYRALPRFGNVKGNEVRVPTLVLWGERDMVLRALQTVG
CHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHC
TTGLESGKESAEFIEPEPVWVPLPTRAEGR
CCCCCCCCCHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]