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The map label for this gene is odhL [H]

Identifier: 94984461

GI number: 94984461

Start: 356938

End: 358344

Strand: Reverse

Name: odhL [H]

Synonym: Dgeo_0353

Alternate gene names: 94984461

Gene position: 358344-356938 (Counterclockwise)

Preceding gene: 94984462

Following gene: 94984459

Centisome position: 14.52

GC content: 65.6

Gene sequence:

>1407_bases
ATGGATGCTTACGACGTGTTGGTGATTGGCGGCGGCCCGGCCGGGTACGTCGCCGCGATTCGTGCCGCTCAGCTCGGGTT
CAAAACCGCCTGTGTGGACGCCTTTACGCGAAATGGCAAGGCCAGCCTGGGCGGCACTTGCCTGAACGTGGGCTGCATCC
CCAGCAAGGCGCTGCTCGATTCCAGCGAGCGCTTCGAGATGATCAAACACGAGGCCGGGGAACACGGCATCCAGGTAGAC
GGCGCCACAGTGGACGTGGCGAAGATGCTGGGCCGCAAAGAAAGCGTGGTGGACAAGCTGACCGGCGGCATCGCCTACCT
CTTCAAGAAGAACAAGGTGACGAGCATCCACGGCCTGGGCCGCCTGGTCCGCCGCGAAGGTGACAGCTGGATCGTGGACG
CAGCAGGGACCGAGGTGCGCGCCAAGAATGTGATCGTGGCGACCGGCAGTACCCCTCGCCAGCTTCCCGGCGTGCCCTTT
GGCGGCAACATCGTGGACAACGAGGGCGCCCTCGCCTTCACGCAGGTGCCCGCCCAGCTCGGCGTGATTGGCGCAGGCGT
GATCGGCGTCGAGCTGGGGAGCGTGTGGCGCCGTCTGGGGGCGCAGGTGACCATTCTGGAGGCCCTTCCCGGCTTCCTGA
TGGCTGCGGACGACGCCGTGAGCAAGGAAGCGCTCAAGCAGTTCCAGAAGCAGGGTCTAGACTTCCACTTTGGTGTGAAG
ATCACGGAGGTCAAGCAGGACGACTCCGGCGTGACCGTCACCTACGAGGAGCAGGGCCAGCCGGTGACCGCCCGCTTCGA
CAAGCTGATCGTGTCCATCGGGCGCGTGCCGAACACCCAGGGCCTGGGCGCCCAGGAGGTGGGCCTCCAGCTCGACGAGC
GCGGCTTCGTGAAGGTGGATCATCACTTCCGCACCAACCTCCCCGGCGTCTATGCCATCGGGGACGTGATCGGCGGCCCG
ATGCTGGCCCACAAGGCCGAAGAAGAAGGGGTGGCGGTGGCCGAGTTGCTGGCCGGACAGGCTGGACATGTCAACTACGC
CGTGGTCCCCTGGGTGATCTACACCAGCCCAGAGATCGCCTGGGCGGGCCTCACCGAGAAACAGGCGAAGGAACAGGGCC
ACAAGGTCAAGACCGGCCAGTTTCCTTTCAGCGCCAATGGCCGCGCGCTGGGTCACGGCGATCCGCGCGGCTTCGTGAAG
GTGGTGGCAGACGCCGACACGGACCGGATTCTGGGCGTTCACATGGTCGGTCCCAATGTCTCCGAACTGATCGGTGAGAC
GGTTGCCCTCATGGAGTTCGGGGCCAGCGCGGAAGACCTCGCCCGCACTGTCCACGCCCACCCCACCCTGTCGGAAGTGG
TGAAGGAAGCGGCGCTCGCGACGGACAAGCGCGCGCTCCACATGTAA

Upstream 100 bases:

>100_bases
CGTCAAACGGTACCGGCGGGCGTGACAGCGACAGGCTGTCCCTGCAACGGGAACGCGCCGCAGTTGGCCGGCGGGGTGGG
TGGTAGGCTCGGGAGTGGTT

Downstream 100 bases:

>100_bases
GAGCCTCAACAGCGTCAAAGGCGCGGGGCGGGAGGCAGCAGCGTTCCCGCCCCTTCTCGTTGGCTGTATCAGACAGACGG
CGTGGACTCGAGCGCCAGGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MDAYDVLVIGGGPAGYVAAIRAAQLGFKTACVDAFTRNGKASLGGTCLNVGCIPSKALLDSSERFEMIKHEAGEHGIQVD
GATVDVAKMLGRKESVVDKLTGGIAYLFKKNKVTSIHGLGRLVRREGDSWIVDAAGTEVRAKNVIVATGSTPRQLPGVPF
GGNIVDNEGALAFTQVPAQLGVIGAGVIGVELGSVWRRLGAQVTILEALPGFLMAADDAVSKEALKQFQKQGLDFHFGVK
ITEVKQDDSGVTVTYEEQGQPVTARFDKLIVSIGRVPNTQGLGAQEVGLQLDERGFVKVDHHFRTNLPGVYAIGDVIGGP
MLAHKAEEEGVAVAELLAGQAGHVNYAVVPWVIYTSPEIAWAGLTEKQAKEQGHKVKTGQFPFSANGRALGHGDPRGFVK
VVADADTDRILGVHMVGPNVSELIGETVALMEFGASAEDLARTVHAHPTLSEVVKEAALATDKRALHM

Sequences:

>Translated_468_residues
MDAYDVLVIGGGPAGYVAAIRAAQLGFKTACVDAFTRNGKASLGGTCLNVGCIPSKALLDSSERFEMIKHEAGEHGIQVD
GATVDVAKMLGRKESVVDKLTGGIAYLFKKNKVTSIHGLGRLVRREGDSWIVDAAGTEVRAKNVIVATGSTPRQLPGVPF
GGNIVDNEGALAFTQVPAQLGVIGAGVIGVELGSVWRRLGAQVTILEALPGFLMAADDAVSKEALKQFQKQGLDFHFGVK
ITEVKQDDSGVTVTYEEQGQPVTARFDKLIVSIGRVPNTQGLGAQEVGLQLDERGFVKVDHHFRTNLPGVYAIGDVIGGP
MLAHKAEEEGVAVAELLAGQAGHVNYAVVPWVIYTSPEIAWAGLTEKQAKEQGHKVKTGQFPFSANGRALGHGDPRGFVK
VVADADTDRILGVHMVGPNVSELIGETVALMEFGASAEDLARTVHAHPTLSEVVKEAALATDKRALHM
>Mature_468_residues
MDAYDVLVIGGGPAGYVAAIRAAQLGFKTACVDAFTRNGKASLGGTCLNVGCIPSKALLDSSERFEMIKHEAGEHGIQVD
GATVDVAKMLGRKESVVDKLTGGIAYLFKKNKVTSIHGLGRLVRREGDSWIVDAAGTEVRAKNVIVATGSTPRQLPGVPF
GGNIVDNEGALAFTQVPAQLGVIGAGVIGVELGSVWRRLGAQVTILEALPGFLMAADDAVSKEALKQFQKQGLDFHFGVK
ITEVKQDDSGVTVTYEEQGQPVTARFDKLIVSIGRVPNTQGLGAQEVGLQLDERGFVKVDHHFRTNLPGVYAIGDVIGGP
MLAHKAEEEGVAVAELLAGQAGHVNYAVVPWVIYTSPEIAWAGLTEKQAKEQGHKVKTGQFPFSANGRALGHGDPRGFVK
VVADADTDRILGVHMVGPNVSELIGETVALMEFGASAEDLARTVHAHPTLSEVVKEAALATDKRALHM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=47.4137931034483, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=28.8172043010753, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=28.5412262156448, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=24.6252676659529, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=24.6252676659529, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=24.6252676659529, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=24.6252676659529, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=24.6252676659529, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI291045266, Length=470, Percent_Identity=25.1063829787234, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=464, Percent_Identity=23.2758620689655, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=41.978021978022, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=29.1571753986333, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=29.4623655913978, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=27.114967462039, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=49.1341991341991, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=27.6923076923077, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=27.6923076923077, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=26.6806722689076, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=25.7322175732218, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=224, Percent_Identity=26.3392857142857, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=46.7640918580376, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=32.0833333333333, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=29.3361884368308, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=48.7012987012987, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=28.6585365853659, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24640549, Length=492, Percent_Identity=29.0650406504065, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24640551, Length=492, Percent_Identity=29.0650406504065, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=27.4261603375527, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49403; Mature: 49403

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAYDVLVIGGGPAGYVAAIRAAQLGFKTACVDAFTRNGKASLGGTCLNVGCIPSKALLD
CCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHC
SSERFEMIKHEAGEHGIQVDGATVDVAKMLGRKESVVDKLTGGIAYLFKKNKVTSIHGLG
CHHHHHHHHHHCCCCCEEECCCEEHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHHH
RLVRREGDSWIVDAAGTEVRAKNVIVATGSTPRQLPGVPFGGNIVDNEGALAFTQVPAQL
HHHHHCCCCEEEECCCCEEEECEEEEECCCCCCCCCCCCCCCEEECCCCCEEEEECCHHH
GVIGAGVIGVELGSVWRRLGAQVTILEALPGFLMAADDAVSKEALKQFQKQGLDFHFGVK
CEEECCHHHHHHHHHHHHHCCCEEEHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEECEE
ITEVKQDDSGVTVTYEEQGQPVTARFDKLIVSIGRVPNTQGLGAQEVGLQLDERGFVKVD
EEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCEECCCCCEEEC
HHFRTNLPGVYAIGDVIGGPMLAHKAEEEGVAVAELLAGQAGHVNYAVVPWVIYTSPEIA
CCHHCCCCCEEEEHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEEEEECCCEE
WAGLTEKQAKEQGHKVKTGQFPFSANGRALGHGDPRGFVKVVADADTDRILGVHMVGPNV
ECCCCHHHHHHCCCEEECCCCCCCCCCCEECCCCCCCEEEEEECCCCCCEEEEEEECCCH
SELIGETVALMEFGASAEDLARTVHAHPTLSEVVKEAALATDKRALHM
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MDAYDVLVIGGGPAGYVAAIRAAQLGFKTACVDAFTRNGKASLGGTCLNVGCIPSKALLD
CCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHC
SSERFEMIKHEAGEHGIQVDGATVDVAKMLGRKESVVDKLTGGIAYLFKKNKVTSIHGLG
CHHHHHHHHHHCCCCCEEECCCEEHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHHH
RLVRREGDSWIVDAAGTEVRAKNVIVATGSTPRQLPGVPFGGNIVDNEGALAFTQVPAQL
HHHHHCCCCEEEECCCCEEEECEEEEECCCCCCCCCCCCCCCEEECCCCCEEEEECCHHH
GVIGAGVIGVELGSVWRRLGAQVTILEALPGFLMAADDAVSKEALKQFQKQGLDFHFGVK
CEEECCHHHHHHHHHHHHHCCCEEEHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEECEE
ITEVKQDDSGVTVTYEEQGQPVTARFDKLIVSIGRVPNTQGLGAQEVGLQLDERGFVKVD
EEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCEECCCCCEEEC
HHFRTNLPGVYAIGDVIGGPMLAHKAEEEGVAVAELLAGQAGHVNYAVVPWVIYTSPEIA
CCHHCCCCCEEEEHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEEEEECCCEE
WAGLTEKQAKEQGHKVKTGQFPFSANGRALGHGDPRGFVKVVADADTDRILGVHMVGPNV
ECCCCHHHHHHCCCEEECCCCCCCCCCCEECCCCCCCEEEEEECCCCCCEEEEEEECCCH
SELIGETVALMEFGASAEDLARTVHAHPTLSEVVKEAALATDKRALHM
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]