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The map label for this gene is murE [H]

Identifier: 94984207

GI number: 94984207

Start: 93423

End: 94889

Strand: Reverse

Name: murE [H]

Synonym: Dgeo_0099

Alternate gene names: 94984207

Gene position: 94889-93423 (Counterclockwise)

Preceding gene: 94984209

Following gene: 94984206

Centisome position: 3.85

GC content: 71.44

Gene sequence:

>1467_bases
ATGCGTCTGCCCGACCTCGCCGCCGCCCTGTCCACGCCCACACCGCCGCTGCCCGACGTGGAGGTGCGCGGCGTCACCCA
CCACGCCGCCTGGGTGGAGCCGGGCTTCCTGTTCGTGGCGATTCGCGGGGCGCGCTTTGACGGGCACAGCTTTCTGCCAG
AGGTGGCAGCCCGGGGGGCAGTGGCCGTGCTGGGCGAGGGGTTGCCGGCGGACGTGGCCTCTCCCCTGCCCTACCTCAGC
GTGCCCAATGCGCGGGCAGCGCTGGCCGACGCAGCGGCAGCCCTGGCCGGACACCCCAGCCGGGCTTTAAAGGTGGTGGG
CGTGACCGGCACCGACGGCAAAACCACGACGAGCTGGCTCACCCGGCATCTGCTGCGTGCAGCCGGCCTGAGGACGGGCC
TCCTCAGCACCGTCGGGTACGAGTTGCCGGACGGGGTGCTGCGGCACTTCCCGGCACATTTCACCACGCCAGAGGCGCCG
CAGGTCCAGACCACGCTGCGCGCGCTGCTGGAGGCAGGCGGAGAGGCCGCCGTGCTGGAAGCGAGCAGCCACGCCCTCGC
GCTCGACCGGGTGCGCGGTGTGGACTGGGACGTGGCGGTCTGGACCCACCTCAGCAGCGAACACCTCGATTTTCACGGCA
CCCTCGAGAACTACTTCGCGGACAAGCGCAAGCTGATCGAGCGCGCGCCCTTCGCCGTGCTGAATGTGGACGATCCCTGG
ACGGCACAACTGCGCGGCGTCGCCCCGCAGGAGACGACCTACAGCGCCGAGAACCAGCACGCCGACTGGCAGGCGCGCCA
CATCGAAGAGCGCGCAACCGGCCTACATTTCCAGGTCGCCTCGCCGCTGGGCGAGTTCGCGGCACACCTGCCGATGATCG
GGCGCTTCAACGTAGCAAACGCGCTCGCGGGGATGGCGGCGGCAGCTCAGCTGGGCGCGACTGTTCCGCAGCTGGTGGAG
GGCCTGGCCTCCTTCCGGGGGGTGCCGGGACGCATGGAACTTGTTCCGGGAGGCGCGAGCGACCCACGAGTGATCGTCGA
TTTCGCGCATACGCCGCCCAGCCTGGAAAAGGCGCTGACCACGCTGCGCGCCACGACGGGCGGGCGGCTGTGGGTGCTGC
TCGGCTCGGCGGGCGGGCCGCGCGACCCGGGCAAGCGGGCCCCGCTGGGCGAGGTGGCGACGCGGCTCGCCGATCACGCG
GTCTTCACCGAGGAGGACTGCCGCGACACGCCCCTCTGGGACATCCTGCGCGAGATGGAGCGGGGCGCACGGGAGGCGGG
CCGGAATAACTTCACCTCCATCGAGGACCGGCGCGAGGCGATCCGCTTCGTGATTCGCGAGGCGCGCCCGGGAGACACGG
TGCTGCTCGCCGGGAAGGGGCCGGAAGACACGTTGGAACGGAACGGCGAGGTCATCGCCTGGAACGAGGTGGAGGAGGCC
CGGGAAGCGCTGGCCGCTCGGGCCTGA

Upstream 100 bases:

>100_bases
ACAGTCAAGATACGCGGGTTGGCGAGGAGAAAAAGGAGCCTCAGCCTCTTCTGTATCCGCTTCTGGCCATCCGCTTCTGG
CCTTCTGCTACCTTCCCCCC

Downstream 100 bases:

>100_bases
CCTCCAGGCATCCCCGGACGCCGGATGGGCAGAACCGAAAGAAGACCCTCAGCCTGTTGGATGACACCGACCAGGCGCAT
CCGCGGGCTGGCTGACAGCG

Product: UDP-N-acetylmuramyl-tripeptide synthetases

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRLPDLAAALSTPTPPLPDVEVRGVTHHAAWVEPGFLFVAIRGARFDGHSFLPEVAARGAVAVLGEGLPADVASPLPYLS
VPNARAALADAAAALAGHPSRALKVVGVTGTDGKTTTSWLTRHLLRAAGLRTGLLSTVGYELPDGVLRHFPAHFTTPEAP
QVQTTLRALLEAGGEAAVLEASSHALALDRVRGVDWDVAVWTHLSSEHLDFHGTLENYFADKRKLIERAPFAVLNVDDPW
TAQLRGVAPQETTYSAENQHADWQARHIEERATGLHFQVASPLGEFAAHLPMIGRFNVANALAGMAAAAQLGATVPQLVE
GLASFRGVPGRMELVPGGASDPRVIVDFAHTPPSLEKALTTLRATTGGRLWVLLGSAGGPRDPGKRAPLGEVATRLADHA
VFTEEDCRDTPLWDILREMERGAREAGRNNFTSIEDRREAIRFVIREARPGDTVLLAGKGPEDTLERNGEVIAWNEVEEA
REALAARA

Sequences:

>Translated_488_residues
MRLPDLAAALSTPTPPLPDVEVRGVTHHAAWVEPGFLFVAIRGARFDGHSFLPEVAARGAVAVLGEGLPADVASPLPYLS
VPNARAALADAAAALAGHPSRALKVVGVTGTDGKTTTSWLTRHLLRAAGLRTGLLSTVGYELPDGVLRHFPAHFTTPEAP
QVQTTLRALLEAGGEAAVLEASSHALALDRVRGVDWDVAVWTHLSSEHLDFHGTLENYFADKRKLIERAPFAVLNVDDPW
TAQLRGVAPQETTYSAENQHADWQARHIEERATGLHFQVASPLGEFAAHLPMIGRFNVANALAGMAAAAQLGATVPQLVE
GLASFRGVPGRMELVPGGASDPRVIVDFAHTPPSLEKALTTLRATTGGRLWVLLGSAGGPRDPGKRAPLGEVATRLADHA
VFTEEDCRDTPLWDILREMERGAREAGRNNFTSIEDRREAIRFVIREARPGDTVLLAGKGPEDTLERNGEVIAWNEVEEA
REALAARA
>Mature_488_residues
MRLPDLAAALSTPTPPLPDVEVRGVTHHAAWVEPGFLFVAIRGARFDGHSFLPEVAARGAVAVLGEGLPADVASPLPYLS
VPNARAALADAAAALAGHPSRALKVVGVTGTDGKTTTSWLTRHLLRAAGLRTGLLSTVGYELPDGVLRHFPAHFTTPEAP
QVQTTLRALLEAGGEAAVLEASSHALALDRVRGVDWDVAVWTHLSSEHLDFHGTLENYFADKRKLIERAPFAVLNVDDPW
TAQLRGVAPQETTYSAENQHADWQARHIEERATGLHFQVASPLGEFAAHLPMIGRFNVANALAGMAAAAQLGATVPQLVE
GLASFRGVPGRMELVPGGASDPRVIVDFAHTPPSLEKALTTLRATTGGRLWVLLGSAGGPRDPGKRAPLGEVATRLADHA
VFTEEDCRDTPLWDILREMERGAREAGRNNFTSIEDRREAIRFVIREARPGDTVLLAGKGPEDTLERNGEVIAWNEVEEA
REALAARA

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=470, Percent_Identity=34.6808510638298, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1786274, Length=374, Percent_Identity=28.0748663101604, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 52246; Mature: 52246

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPDLAAALSTPTPPLPDVEVRGVTHHAAWVEPGFLFVAIRGARFDGHSFLPEVAARGA
CCCCHHHHHHCCCCCCCCCCEECCCEEHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCC
VAVLGEGLPADVASPLPYLSVPNARAALADAAAALAGHPSRALKVVGVTGTDGKTTTSWL
EEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
TRHLLRAAGLRTGLLSTVGYELPDGVLRHFPAHFTTPEAPQVQTTLRALLEAGGEAAVLE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
ASSHALALDRVRGVDWDVAVWTHLSSEHLDFHGTLENYFADKRKLIERAPFAVLNVDDPW
CCCCHHHHHHHCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
TAQLRGVAPQETTYSAENQHADWQARHIEERATGLHFQVASPLGEFAAHLPMIGRFNVAN
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCHHH
ALAGMAAAAQLGATVPQLVEGLASFRGVPGRMELVPGGASDPRVIVDFAHTPPSLEKALT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHH
TLRATTGGRLWVLLGSAGGPRDPGKRAPLGEVATRLADHAVFTEEDCRDTPLWDILREME
HHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
RGAREAGRNNFTSIEDRREAIRFVIREARPGDTVLLAGKGPEDTLERNGEVIAWNEVEEA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCEEEECHHHHH
REALAARA
HHHHHCCC
>Mature Secondary Structure
MRLPDLAAALSTPTPPLPDVEVRGVTHHAAWVEPGFLFVAIRGARFDGHSFLPEVAARGA
CCCCHHHHHHCCCCCCCCCCEECCCEEHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCC
VAVLGEGLPADVASPLPYLSVPNARAALADAAAALAGHPSRALKVVGVTGTDGKTTTSWL
EEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
TRHLLRAAGLRTGLLSTVGYELPDGVLRHFPAHFTTPEAPQVQTTLRALLEAGGEAAVLE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
ASSHALALDRVRGVDWDVAVWTHLSSEHLDFHGTLENYFADKRKLIERAPFAVLNVDDPW
CCCCHHHHHHHCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
TAQLRGVAPQETTYSAENQHADWQARHIEERATGLHFQVASPLGEFAAHLPMIGRFNVAN
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCHHH
ALAGMAAAAQLGATVPQLVEGLASFRGVPGRMELVPGGASDPRVIVDFAHTPPSLEKALT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHH
TLRATTGGRLWVLLGSAGGPRDPGKRAPLGEVATRLADHAVFTEEDCRDTPLWDILREME
HHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
RGAREAGRNNFTSIEDRREAIRFVIREARPGDTVLLAGKGPEDTLERNGEVIAWNEVEEA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCEEEECHHHHH
REALAARA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]