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The map label for this gene is purF [H]

Identifier: 94984171

GI number: 94984171

Start: 60394

End: 61812

Strand: Reverse

Name: purF [H]

Synonym: Dgeo_0063

Alternate gene names: 94984171

Gene position: 61812-60394 (Counterclockwise)

Preceding gene: 94984172

Following gene: 94984164

Centisome position: 2.51

GC content: 67.58

Gene sequence:

>1419_bases
ATGATCTTTGACCCCGCCACCGACAAGCCGCAAGACGAGTGCGGGGTGTTCGGCCTGTACTCGCCGCAGCCGCAGGATCT
CGCGTGGCTGACGTACCTGGGTCTCTTCGCCCTGCAGCACCGCGGGCAAGAGGCGGCGGGCATGTGCGTCTCAGACGGCG
AAAAGTTCCACGTCGACAAGGACCTGGGGCTGGTCTCGCAGGTCTTCGACGAGCGGCGGCTGGACGGCCTGCGCCTCCCC
AACGCCCGCGTGAGCATCGGCCACGTGCGCTACTCCACCACCGGCTCCAACCTGCGCTTCAACGCCCAGCCCCTCACCAC
CCGCACCAACAAGGGCATCCTGGGGCTGGCGCACAACGGCAACTTCGTGAATGCCCGCGAGGTGCGCAGCGGCATGCTGC
TGGAGGGGGCGCTCTTTCAGACCACCAACGACTCCGAAGTGATGCTCAACCTGATCGCGCGCGAGTCACACATGGACCTG
GTGGAGGCGACCGCCTCGGCCATGCGGAAGCTGAAGGGCGGCTATGCCTGCGTGCTGATGAGCCGTCACACGCTGCTCGG
CTTCCGCGACCCGCACGGCGTGCGGCCCCTGGTGATTGGCCAGCGCAACGACGGCGCGTGGGTGCTGGCCTCTGAGCCTT
GCGCCCTGTACGCCGTGGGCGCCCGACTCCTGCGCGACGTGCAGCCCGGTGAGCTGGTGTGGTTTGACCGCGAGGGGCTG
CACTCGCTGCTGGTAGAAGTGAAAACGCCTACCCCTTGTTCTTTCGAGTGGATCTACTTTGCGCGCAGCGACGGCGAACT
CGACGGCGTGGACATCCACGAGAGCCGCCTGAGGATGGGCGCGCAGCTCGCCCGCGAGAAGCCGGTGGACGCCGATCTCG
TGGTGCCGGTTCCCGACTCCGGCATCGGCGCGGCCATCGGGTACGCCCGCGAGAGTGGGATTCCCTTCGACTACGGCCTG
TACAAGAACCCCTACGCGGGCCGCACCTTTATCGCCCCCACCCAGGAGGCGCGCGAGCTGAAGGTGAAGATGAAGCTCTC
CCCCACCAGCGCCGTCCGGGGGCGGCGGGTTGTGCTGGTGGACGACTCCATCGTGCGCGGCACCACCAGCCGCCAGATCG
TGAACCTGCTGCGCGAGGCGGGGGCGCGAGAGGTGCATTTTCGCGTGTCCAGCCCGCCCATCACCCATCCGTGCTTCTAC
GGCATCGACACCGCCGCTCGCAAAGAACTCGTCGCGAGCACGCACAGCGTCGAGGAAATCCGGGAACTGATCGGGGCGGA
CACCCTCGCCTTTATCAGCGAGCGCGGCCTGCGCGAGGCCATCGGTGGCCCCGGCCTGTGTTCGGCCTGCTTCACGGGGG
ACTACCCGGCGGGAACACCGCTGCTGAACGATGTGGACAAGCTGGCCCTGGAGGTGTGA

Upstream 100 bases:

>100_bases
ACCGCGTCACCATTTCGGTCACGGGCGCAAACGTACAGTTGAGCGTGACCCTCGACACCCTCAAAACTGCCTTTGAAACG
CCGCTGCGGGAGATTTTGGG

Downstream 100 bases:

>100_bases
TCCAGGGTGGCCTTGATTTCAATCCATCCTGGAAACAATACCGACATCGGTCTCCATCGTCGCCCTCCCGTAGGTTCTTC
TCTTCGCGCTGCTGCGCCGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIFDPATDKPQDECGVFGLYSPQPQDLAWLTYLGLFALQHRGQEAAGMCVSDGEKFHVDKDLGLVSQVFDERRLDGLRLP
NARVSIGHVRYSTTGSNLRFNAQPLTTRTNKGILGLAHNGNFVNAREVRSGMLLEGALFQTTNDSEVMLNLIARESHMDL
VEATASAMRKLKGGYACVLMSRHTLLGFRDPHGVRPLVIGQRNDGAWVLASEPCALYAVGARLLRDVQPGELVWFDREGL
HSLLVEVKTPTPCSFEWIYFARSDGELDGVDIHESRLRMGAQLAREKPVDADLVVPVPDSGIGAAIGYARESGIPFDYGL
YKNPYAGRTFIAPTQEARELKVKMKLSPTSAVRGRRVVLVDDSIVRGTTSRQIVNLLREAGAREVHFRVSSPPITHPCFY
GIDTAARKELVASTHSVEEIRELIGADTLAFISERGLREAIGGPGLCSACFTGDYPAGTPLLNDVDKLALEV

Sequences:

>Translated_472_residues
MIFDPATDKPQDECGVFGLYSPQPQDLAWLTYLGLFALQHRGQEAAGMCVSDGEKFHVDKDLGLVSQVFDERRLDGLRLP
NARVSIGHVRYSTTGSNLRFNAQPLTTRTNKGILGLAHNGNFVNAREVRSGMLLEGALFQTTNDSEVMLNLIARESHMDL
VEATASAMRKLKGGYACVLMSRHTLLGFRDPHGVRPLVIGQRNDGAWVLASEPCALYAVGARLLRDVQPGELVWFDREGL
HSLLVEVKTPTPCSFEWIYFARSDGELDGVDIHESRLRMGAQLAREKPVDADLVVPVPDSGIGAAIGYARESGIPFDYGL
YKNPYAGRTFIAPTQEARELKVKMKLSPTSAVRGRRVVLVDDSIVRGTTSRQIVNLLREAGAREVHFRVSSPPITHPCFY
GIDTAARKELVASTHSVEEIRELIGADTLAFISERGLREAIGGPGLCSACFTGDYPAGTPLLNDVDKLALEV
>Mature_472_residues
MIFDPATDKPQDECGVFGLYSPQPQDLAWLTYLGLFALQHRGQEAAGMCVSDGEKFHVDKDLGLVSQVFDERRLDGLRLP
NARVSIGHVRYSTTGSNLRFNAQPLTTRTNKGILGLAHNGNFVNAREVRSGMLLEGALFQTTNDSEVMLNLIARESHMDL
VEATASAMRKLKGGYACVLMSRHTLLGFRDPHGVRPLVIGQRNDGAWVLASEPCALYAVGARLLRDVQPGELVWFDREGL
HSLLVEVKTPTPCSFEWIYFARSDGELDGVDIHESRLRMGAQLAREKPVDADLVVPVPDSGIGAAIGYARESGIPFDYGL
YKNPYAGRTFIAPTQEARELKVKMKLSPTSAVRGRRVVLVDDSIVRGTTSRQIVNLLREAGAREVHFRVSSPPITHPCFY
GIDTAARKELVASTHSVEEIRELIGADTLAFISERGLREAIGGPGLCSACFTGDYPAGTPLLNDVDKLALEV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=38.4615384615385, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1788651, Length=478, Percent_Identity=39.7489539748954, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1790167, Length=221, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=39.1304347826087, Blast_Score=318, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6323958, Length=477, Percent_Identity=37.7358490566038, Blast_Score=278, Evalue=9e-76,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=40.1197604790419, Blast_Score=345, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24659598, Length=498, Percent_Identity=39.3574297188755, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24659604, Length=477, Percent_Identity=39.4129979035639, Blast_Score=332, Evalue=5e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51686; Mature: 51686

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDPATDKPQDECGVFGLYSPQPQDLAWLTYLGLFALQHRGQEAAGMCVSDGEKFHVDK
CCCCCCCCCCCHHCCEEECCCCCCCCHHHHHHHHHHHHHHCCCHHCCCEECCCCEEEECC
DLGLVSQVFDERRLDGLRLPNARVSIGHVRYSTTGSNLRFNAQPLTTRTNKGILGLAHNG
HHHHHHHHHHHHCCCCEECCCCEEEEEEEEEECCCCCEEECCCCCCEECCCCEEEEECCC
NFVNAREVRSGMLLEGALFQTTNDSEVMLNLIARESHMDLVEATASAMRKLKGGYACVLM
CEEEHHHHHCCCEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
SRHTLLGFRDPHGVRPLVIGQRNDGAWVLASEPCALYAVGARLLRDVQPGELVWFDREGL
ECCEEEEECCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEECHHCH
HSLLVEVKTPTPCSFEWIYFARSDGELDGVDIHESRLRMGAQLAREKPVDADLVVPVPDS
HEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCC
GIGAAIGYARESGIPFDYGLYKNPYAGRTFIAPTQEARELKVKMKLSPTSAVRGRRVVLV
CCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCHHHCCCEEEEE
DDSIVRGTTSRQIVNLLREAGAREVHFRVSSPPITHPCFYGIDTAARKELVASTHSVEEI
CCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHH
RELIGADTLAFISERGLREAIGGPGLCSACFTGDYPAGTPLLNDVDKLALEV
HHHHCCHHHHHHHHCCHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIFDPATDKPQDECGVFGLYSPQPQDLAWLTYLGLFALQHRGQEAAGMCVSDGEKFHVDK
CCCCCCCCCCCHHCCEEECCCCCCCCHHHHHHHHHHHHHHCCCHHCCCEECCCCEEEECC
DLGLVSQVFDERRLDGLRLPNARVSIGHVRYSTTGSNLRFNAQPLTTRTNKGILGLAHNG
HHHHHHHHHHHHCCCCEECCCCEEEEEEEEEECCCCCEEECCCCCCEECCCCEEEEECCC
NFVNAREVRSGMLLEGALFQTTNDSEVMLNLIARESHMDLVEATASAMRKLKGGYACVLM
CEEEHHHHHCCCEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
SRHTLLGFRDPHGVRPLVIGQRNDGAWVLASEPCALYAVGARLLRDVQPGELVWFDREGL
ECCEEEEECCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEECHHCH
HSLLVEVKTPTPCSFEWIYFARSDGELDGVDIHESRLRMGAQLAREKPVDADLVVPVPDS
HEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCC
GIGAAIGYARESGIPFDYGLYKNPYAGRTFIAPTQEARELKVKMKLSPTSAVRGRRVVLV
CCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCHHHCCCEEEEE
DDSIVRGTTSRQIVNLLREAGAREVHFRVSSPPITHPCFYGIDTAARKELVASTHSVEEI
CCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHH
RELIGADTLAFISERGLREAIGGPGLCSACFTGDYPAGTPLLNDVDKLALEV
HHHHCCHHHHHHHHCCHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]