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The map label for this gene is mdeA [H]

Identifier: 94984120

GI number: 94984120

Start: 11803

End: 13008

Strand: Reverse

Name: mdeA [H]

Synonym: Dgeo_0011

Alternate gene names: 94984120

Gene position: 13008-11803 (Counterclockwise)

Preceding gene: 94984121

Following gene: 94984119

Centisome position: 0.53

GC content: 69.07

Gene sequence:

>1206_bases
ATGGGCACCATGTCTAAACAAGAAAGCCAGGGGTTCCGGACCCGGGCCGTTCATGCGGGGCATGGCCTTGACCCAGTGAC
CGGCGCGCACGCGGTGCCCATCTACGCCACCTCTACCTTCGGCTACGGCAATGCTGAGCGCGGCGCCCGCCTCTTTGCCG
GGGAGGAACCGGGCTACTTCTACTCGCGCCTTTCCAATCCCACAGTGCGGGCCTTCGAGGAGAAGATCGCCAGCTTGGAA
GAGGCCGGGGACGCGGTGGCCTTCGGGAGCGGCATGGGTGCAGCCAGTGCCGTCGCCCTCACCTTGCTGAAGGCCGGGGA
CGCGGTGGCCTTTGTCGGGCCGCTGTACGGCGGGACCGAGGGGTTACTGCGCGACATCCTGGGCCGCTTCGGCGTGACGG
TCCACGAGGCGCGCGACGTGGAGGAGTTGCGCGCCCTGGTATCGGACCGAACGCGGCTGGTGTGGCTGGAGACGCCCACC
AATCCGACCCTGAAGGTGGTGGACCTGCGGGCGGCCGCGAAGGTGGCGCACGCGGCCGGCGCGCTGGTCGTGGTGGACAA
CACCTTTTCCACGCCGTACCTCACCCGCCCGCTGGAGTTCGGGGCCGATCTGGTGATGCACTCCGCGACCAAGTACCTGG
GCGGACACGGCGACGTGATCGCGGGCGTGGTTGCCGGGAGCACCGACCTGGTCGCCGAACTGCGCCTGCACGGGCTGCGG
CACGTCGGGGCGGTGCTTGGACCTTTCGAGGCCTACCTGTTGCTGCGCGGCCTGAAGACCCTGCCGCTGCGGATGGAGGC
TCACTGTGCCAATGCCCAGGCGCTCGCCGAAGCCCTGCAGGACCACCCGGCCCTCCAAGCCCTGCACTACCCCGGCCTGC
AAGGGCACCCTGGCCACGAGGTCGCCGCTCGCCAGATGCGCGCTTTTGGCGGCTTGGTGAGCCTTGATCTCGGCTCGCAG
GAGGCGGCCTTTGCCTTCCTCGACCACCTCAAGCTCTTCACGCAGGCCGTCAGCCTGGGCGACGTGGAAAGCCTCTCCTC
CCACCCCGCCAGCACCACCCACCAGCTGCTGGGCGAGGAAACGCTGGCGCGTCAGGGCATCACGCCCGGCCTGGTGCGCC
TCTCGGTCGGCATCGAGGATCCTGAGGATCTGATTCGGGACGTGCTGGGCGCCCTCGAAAAGGTGCCGGTGAGCGCGGGG
CGCTGA

Upstream 100 bases:

>100_bases
CGTCATGCTGCTGCGCTGGCTCTCGCGCCGGGGCTGGGTCAGATTGCCGGTGCGGCGGCTGCCGGGAGAGTGAAGCGCGA
CGAGCAAGCCGCACAGTAGA

Downstream 100 bases:

>100_bases
GTGAGCAGGGGTGGGGCAGGCACCGCCTGCGCGCTTCGCTCCACCTCACAGGCATGAGCATCTTGCACACATCACTCAAA
TAAAGGTGCTATGCTCACTC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MGTMSKQESQGFRTRAVHAGHGLDPVTGAHAVPIYATSTFGYGNAERGARLFAGEEPGYFYSRLSNPTVRAFEEKIASLE
EAGDAVAFGSGMGAASAVALTLLKAGDAVAFVGPLYGGTEGLLRDILGRFGVTVHEARDVEELRALVSDRTRLVWLETPT
NPTLKVVDLRAAAKVAHAAGALVVVDNTFSTPYLTRPLEFGADLVMHSATKYLGGHGDVIAGVVAGSTDLVAELRLHGLR
HVGAVLGPFEAYLLLRGLKTLPLRMEAHCANAQALAEALQDHPALQALHYPGLQGHPGHEVAARQMRAFGGLVSLDLGSQ
EAAFAFLDHLKLFTQAVSLGDVESLSSHPASTTHQLLGEETLARQGITPGLVRLSVGIEDPEDLIRDVLGALEKVPVSAG
R

Sequences:

>Translated_401_residues
MGTMSKQESQGFRTRAVHAGHGLDPVTGAHAVPIYATSTFGYGNAERGARLFAGEEPGYFYSRLSNPTVRAFEEKIASLE
EAGDAVAFGSGMGAASAVALTLLKAGDAVAFVGPLYGGTEGLLRDILGRFGVTVHEARDVEELRALVSDRTRLVWLETPT
NPTLKVVDLRAAAKVAHAAGALVVVDNTFSTPYLTRPLEFGADLVMHSATKYLGGHGDVIAGVVAGSTDLVAELRLHGLR
HVGAVLGPFEAYLLLRGLKTLPLRMEAHCANAQALAEALQDHPALQALHYPGLQGHPGHEVAARQMRAFGGLVSLDLGSQ
EAAFAFLDHLKLFTQAVSLGDVESLSSHPASTTHQLLGEETLARQGITPGLVRLSVGIEDPEDLIRDVLGALEKVPVSAG
R
>Mature_400_residues
GTMSKQESQGFRTRAVHAGHGLDPVTGAHAVPIYATSTFGYGNAERGARLFAGEEPGYFYSRLSNPTVRAFEEKIASLEE
AGDAVAFGSGMGAASAVALTLLKAGDAVAFVGPLYGGTEGLLRDILGRFGVTVHEARDVEELRALVSDRTRLVWLETPTN
PTLKVVDLRAAAKVAHAAGALVVVDNTFSTPYLTRPLEFGADLVMHSATKYLGGHGDVIAGVVAGSTDLVAELRLHGLRH
VGAVLGPFEAYLLLRGLKTLPLRMEAHCANAQALAEALQDHPALQALHYPGLQGHPGHEVAARQMRAFGGLVSLDLGSQE
AAFAFLDHLKLFTQAVSLGDVESLSSHPASTTHQLLGEETLARQGITPGLVRLSVGIEDPEDLIRDVLGALEKVPVSAGR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=374, Percent_Identity=41.1764705882353, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI299473759, Length=374, Percent_Identity=37.1657754010695, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=35.8490566037736, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=41.9525065963061, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=28.1329923273657, Blast_Score=141, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=382, Percent_Identity=39.2670157068063, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17538055, Length=375, Percent_Identity=40.2666666666667, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17560056, Length=376, Percent_Identity=40.6914893617021, Blast_Score=254, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=41.645885286783, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=34.8623853211009, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=29.9492385786802, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321254, Length=391, Percent_Identity=31.4578005115089, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=265, Percent_Identity=29.811320754717, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6322970, Length=292, Percent_Identity=26.3698630136986, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922554, Length=393, Percent_Identity=40.7124681933842, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42266; Mature: 42134

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTMSKQESQGFRTRAVHAGHGLDPVTGAHAVPIYATSTFGYGNAERGARLFAGEEPGYF
CCCCCCCHHCCCHHEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCHH
YSRLSNPTVRAFEEKIASLEEAGDAVAFGSGMGAASAVALTLLKAGDAVAFVGPLYGGTE
HHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCHH
GLLRDILGRFGVTVHEARDVEELRALVSDRTRLVWLETPTNPTLKVVDLRAAAKVAHAAG
HHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHCC
ALVVVDNTFSTPYLTRPLEFGADLVMHSATKYLGGHGDVIAGVVAGSTDLVAELRLHGLR
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHH
HVGAVLGPFEAYLLLRGLKTLPLRMEAHCANAQALAEALQDHPALQALHYPGLQGHPGHE
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHCHHHHHHCCCCCCCCCCHH
VAARQMRAFGGLVSLDLGSQEAAFAFLDHLKLFTQAVSLGDVESLSSHPASTTHQLLGEE
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHH
TLARQGITPGLVRLSVGIEDPEDLIRDVLGALEKVPVSAGR
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GTMSKQESQGFRTRAVHAGHGLDPVTGAHAVPIYATSTFGYGNAERGARLFAGEEPGYF
CCCCCCHHCCCHHEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCHH
YSRLSNPTVRAFEEKIASLEEAGDAVAFGSGMGAASAVALTLLKAGDAVAFVGPLYGGTE
HHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCHH
GLLRDILGRFGVTVHEARDVEELRALVSDRTRLVWLETPTNPTLKVVDLRAAAKVAHAAG
HHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHCC
ALVVVDNTFSTPYLTRPLEFGADLVMHSATKYLGGHGDVIAGVVAGSTDLVAELRLHGLR
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHH
HVGAVLGPFEAYLLLRGLKTLPLRMEAHCANAQALAEALQDHPALQALHYPGLQGHPGHE
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCHHHHHHHHHHCHHHHHHCCCCCCCCCCHH
VAARQMRAFGGLVSLDLGSQEAAFAFLDHLKLFTQAVSLGDVESLSSHPASTTHQLLGEE
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHH
TLARQGITPGLVRLSVGIEDPEDLIRDVLGALEKVPVSAGR
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]