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The map label for this gene is mls [H]

Identifier: 94972079

GI number: 94972079

Start: 441046

End: 442428

Strand: Direct

Name: mls [H]

Synonym: Dgeo_2611

Alternate gene names: 94972079

Gene position: 441046-442428 (Clockwise)

Preceding gene: 94972086

Following gene: 94972078

Centisome position: 76.82

GC content: 71.44

Gene sequence:

>1383_bases
GTGACCCTACCCGGTACCGACCTGTCCCGCGCCCTGGAGGCCCGCTTCTCGCAGCGCCGCCGCGCCCTGCTCGCCCGCCC
CCGGGACGGCTGGACACCCGGCTTCGATCCCCGCACCGCGGCCATTCGCCAGGCCGACTGGCAGGTGGCGCCCCCGCCCG
CTGAACTGCGGGGGCGAGTGGTGGAACAACTGGTGGAACCCAGCGACGCGGCGGCGATTCAAGCCGCGCTGGAGGCTGGC
CCCGACGCGCTGATCTTCGACTTCGACGACACCTTCTCCCCCACCCCCGCGAATGTCCGCGCGGGGCACGCCAACCTGCG
CACGCTGCCCACGGCGGGCGGCCCGCTCCCGATGACCCGGCCCCGGCCCCTCTACCTGGAGGACGGGCAGGGGGCCAGCG
CCAGCCTTCAGGACCTGGCCGCCTTTGTGGAGGCGTTTGGAGACCGGGAAACCCTGTACGTCTACCTCCCCAAGCTGGAA
TTCCCCGAAGAGGCCGAGTTCTGGCAGGACCTGCTGACCGAAACCGAGCGGCTCACGGGGCGCGCGCCGGGCAGCCTCCG
GGTCTGTCTTCAGATCGAGACGCTGCCCGGTGCCTTCTATGCCGACGAGCTGTTGTACGCGCTGCGGGAACGGGCCTTTG
GGCTGAACGCGGGGCGCTGGGATTACGTGTTCAGCGCGGTCAAGTGGCTGAGCCAGGATCGCCGCTTCTGCCTGCCGGAA
CGCGGCAAGCTGAACATGGATCAGCCCTCCATGCGCGCCTACGAGCAGCACCTGGCCCGCGTGTGTGCCCGGCGGGGCGC
GCAGGCCATCGGCGGAACCGCTGCACTGGCGCCCGATCCCGCCGATCCCGAACCCGCGCTGGCGGTGGTCCGCGCCGACA
AGGCTCGCGAGGCGGCGCAAGGCTTTGTCGCGGCTTGGGCCGGGTTGCCAAGTCTGATTCCGACCGTCCGGGCTGTGTTC
CAGTCCACCCCACCCGCCGCGCCGCCTGCGCCAAAGCCAGAAGAGGAGGTCGCCTCGGCGCTGCTGGCCTTCCCCCGCGT
GGTCGAGGTACCGCTGTCCGCCGTGCGCGAGGCCATCGGCGTGGCGACGGACTATTTCCGAGCCTGGTTGGCCGGACAGG
GCGTGATCGTGCGGCAGCATCGGGTAGAGGACACCGCGACCGCCGAACTCGCCCGCGCGCAACTGTGGCAATGGGTGAGA
CACCGCGTCCCGCTGGAAACCGGCGAGGTGCTGACGCCCGAACGCTTCGGGGCACTGCTGACGGAACAGGCCGACCCCCA
GGAACCCGCCGCCCGGTTGCTGCGCGCGCTGGTCCTCTCCGAGACCTGCCCGCCCTTCTTTCCCGCCGCCGCCCGCACCC
TCGACGAGGTTCCGCTCGCATGA

Upstream 100 bases:

>100_bases
GGTCTCGGAACGTGTTAGACCTTCTCTCACTGCGCCTGCCCCTGTCCGCCGCCCTTTCTCCGGGAGAACCCGTTCGCCTG
CCGGCAGCTCTGGAGACACT

Downstream 100 bases:

>100_bases
CCACTTCCCCGCTGCCCCACACCCCCGACTGGGCCGACCTGGCCGGGCGCACCCTCGCCTGCTGCCTGGAGATCGCCGCC
TGCACCGAGGAACCCGGCCA

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTLPGTDLSRALEARFSQRRRALLARPRDGWTPGFDPRTAAIRQADWQVAPPPAELRGRVVEQLVEPSDAAAIQAALEAG
PDALIFDFDDTFSPTPANVRAGHANLRTLPTAGGPLPMTRPRPLYLEDGQGASASLQDLAAFVEAFGDRETLYVYLPKLE
FPEEAEFWQDLLTETERLTGRAPGSLRVCLQIETLPGAFYADELLYALRERAFGLNAGRWDYVFSAVKWLSQDRRFCLPE
RGKLNMDQPSMRAYEQHLARVCARRGAQAIGGTAALAPDPADPEPALAVVRADKAREAAQGFVAAWAGLPSLIPTVRAVF
QSTPPAAPPAPKPEEEVASALLAFPRVVEVPLSAVREAIGVATDYFRAWLAGQGVIVRQHRVEDTATAELARAQLWQWVR
HRVPLETGEVLTPERFGALLTEQADPQEPAARLLRALVLSETCPPFFPAAARTLDEVPLA

Sequences:

>Translated_460_residues
MTLPGTDLSRALEARFSQRRRALLARPRDGWTPGFDPRTAAIRQADWQVAPPPAELRGRVVEQLVEPSDAAAIQAALEAG
PDALIFDFDDTFSPTPANVRAGHANLRTLPTAGGPLPMTRPRPLYLEDGQGASASLQDLAAFVEAFGDRETLYVYLPKLE
FPEEAEFWQDLLTETERLTGRAPGSLRVCLQIETLPGAFYADELLYALRERAFGLNAGRWDYVFSAVKWLSQDRRFCLPE
RGKLNMDQPSMRAYEQHLARVCARRGAQAIGGTAALAPDPADPEPALAVVRADKAREAAQGFVAAWAGLPSLIPTVRAVF
QSTPPAAPPAPKPEEEVASALLAFPRVVEVPLSAVREAIGVATDYFRAWLAGQGVIVRQHRVEDTATAELARAQLWQWVR
HRVPLETGEVLTPERFGALLTEQADPQEPAARLLRALVLSETCPPFFPAAARTLDEVPLA
>Mature_459_residues
TLPGTDLSRALEARFSQRRRALLARPRDGWTPGFDPRTAAIRQADWQVAPPPAELRGRVVEQLVEPSDAAAIQAALEAGP
DALIFDFDDTFSPTPANVRAGHANLRTLPTAGGPLPMTRPRPLYLEDGQGASASLQDLAAFVEAFGDRETLYVYLPKLEF
PEEAEFWQDLLTETERLTGRAPGSLRVCLQIETLPGAFYADELLYALRERAFGLNAGRWDYVFSAVKWLSQDRRFCLPER
GKLNMDQPSMRAYEQHLARVCARRGAQAIGGTAALAPDPADPEPALAVVRADKAREAAQGFVAAWAGLPSLIPTVRAVFQ
STPPAAPPAPKPEEEVASALLAFPRVVEVPLSAVREAIGVATDYFRAWLAGQGVIVRQHRVEDTATAELARAQLWQWVRH
RVPLETGEVLTPERFGALLTEQADPQEPAARLLRALVLSETCPPFFPAAARTLDEVPLA

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=448, Percent_Identity=33.2589285714286, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17561814, Length=470, Percent_Identity=33.1914893617021, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71982926, Length=356, Percent_Identity=33.7078651685393, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6324212, Length=460, Percent_Identity=33.2608695652174, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322222, Length=460, Percent_Identity=33.695652173913, Blast_Score=223, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 50297; Mature: 50165

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPGTDLSRALEARFSQRRRALLARPRDGWTPGFDPRTAAIRQADWQVAPPPAELRGRV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHH
VEQLVEPSDAAAIQAALEAGPDALIFDFDDTFSPTPANVRAGHANLRTLPTAGGPLPMTR
HHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
PRPLYLEDGQGASASLQDLAAFVEAFGDRETLYVYLPKLEFPEEAEFWQDLLTETERLTG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
RAPGSLRVCLQIETLPGAFYADELLYALRERAFGLNAGRWDYVFSAVKWLSQDRRFCLPE
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
RGKLNMDQPSMRAYEQHLARVCARRGAQAIGGTAALAPDPADPEPALAVVRADKAREAAQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCEECCCCCCCCCCCEEEEECHHHHHHHH
GFVAAWAGLPSLIPTVRAVFQSTPPAAPPAPKPEEEVASALLAFPRVVEVPLSAVREAIG
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATDYFRAWLAGQGVIVRQHRVEDTATAELARAQLWQWVRHRVPLETGEVLTPERFGALL
HHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TEQADPQEPAARLLRALVLSETCPPFFPAAARTLDEVPLA
HCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TLPGTDLSRALEARFSQRRRALLARPRDGWTPGFDPRTAAIRQADWQVAPPPAELRGRV
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHH
VEQLVEPSDAAAIQAALEAGPDALIFDFDDTFSPTPANVRAGHANLRTLPTAGGPLPMTR
HHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
PRPLYLEDGQGASASLQDLAAFVEAFGDRETLYVYLPKLEFPEEAEFWQDLLTETERLTG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
RAPGSLRVCLQIETLPGAFYADELLYALRERAFGLNAGRWDYVFSAVKWLSQDRRFCLPE
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
RGKLNMDQPSMRAYEQHLARVCARRGAQAIGGTAALAPDPADPEPALAVVRADKAREAAQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCEECCCCCCCCCCCEEEEECHHHHHHHH
GFVAAWAGLPSLIPTVRAVFQSTPPAAPPAPKPEEEVASALLAFPRVVEVPLSAVREAIG
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATDYFRAWLAGQGVIVRQHRVEDTATAELARAQLWQWVRHRVPLETGEVLTPERFGALL
HHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TEQADPQEPAARLLRALVLSETCPPFFPAAARTLDEVPLA
HCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA