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The map label for this gene is arsB [H]

Identifier: 94972015

GI number: 94972015

Start: 502551

End: 503852

Strand: Direct

Name: arsB [H]

Synonym: Dgeo_2547

Alternate gene names: 94972015

Gene position: 502551-503852 (Clockwise)

Preceding gene: 94972016

Following gene: 94972014

Centisome position: 87.53

GC content: 67.43

Gene sequence:

>1302_bases
ATGCTGCTCGCCGTCCTGATCTTCCTGTTCACCCTCGTCCTCGTCATCTGGCAGCCGAAGCTCAGGTGGCAACCAGGGGG
CCTGGGCATCGGCTGGAGTGCGTCACTCGGCGCGGTCCTCGCCCTGCTCACCGGGGTCGTCCACCTCGCGGACATTCCGG
TGGTGTGGAACATCGTGTGGAACGCGACCATCACCTTCGTTGCCCTCATCATCATCAGCCTGATCCTCGACGAGGCCGGG
TTCTTCAAGTGGTCTGCCCTGCACGTGGCCCGCTGGGGCCGCGGGCACGGCCACCTGCTCTTTGCTCTGGTGATCCTGTT
GGGTGCCGCCGTGAGTGCCCTGTTCGCCAACGACGGCACGGCGCTGATCCTCACGCCCATCGTGCTCGCCATGCTCACCG
CGCTGGGCTTCCGGCCCGCCACCACCCTCGCGTTCATCCTCGCCACGGGGTTCATCGCCGACAGTGCCAGCCTGCCGCTG
GTCATCAGCAACCTGGTGAACATTGTCAGCGCCGACTACTTCAACCTGGACTTCGGACAGTACGCCAGGGTGATGGTGCC
GGTGGACCTCGCGGCGATCCTCGCCAGTCTCGGCGTGCTGTACGTGATGTTCCGCCGCGATCTGCCTGCGCGTTACGACC
CAGGCACGCTGGGAACGCCTGCCCAGGCCATCCGTGACCCCAACGTCTTCCGGGTGGGCTGGATCGTCCTGGTGGTCCTG
CTGGTCGGGTACTTCGCCGCCGGGCCGCTCGGGGTGCCCGTCAGCCTGGTCGCGGCGCTGGGGGCAGGCCTCCTGTGGCT
CGTCGCCGCTCGTGGGCACGTCGTGAGCACCCGGAACGTCCTCAGGGGCGCGCCCTGGCAGATCGTCATTTTCTCGCTGG
GCATGTACCTGGTCGTGTACGGTCTGCGAAACGCCGGGCTGACCGACTTGCTGGCGGGCGTCCTTGACCGACTGGCTCAG
GGCGGACTTTGGAGCGCCACCCTCGGCACCGGCTTTCTGACCGCCTTCCTCGCCAGTGTGATGAACAACATGCCCAGCGT
CCTGATCGGCGCGCTCGCCATCGACGCCAGCCAGGCCACCGGAGCCGTCAAGCAGGGCATGGTCTACGCGAATGTCGTCG
GCAACGACCTGGGGCCGAAGATCACGCCCATCGGGAGCCTCGCCACGCTGCTGTGGCTGCACGTGCTGGCCAGCAAGGGG
ATCCGCATCGGGTGGGGCCAGTATTTCCGGGTCGGGATCGTCCTCACGCTGCCGGTGCTGCTGGTCACGCTCGCGGCGCT
CGCGCTGCGCCTGGGAGGCTGA

Upstream 100 bases:

>100_bases
TTCAGGGGCAGTTCGAGGCCTTCGTGCAGGCCCTCAAGGATGGGCAGCCCGTGCCCGAGAGCTACGAGGACAGCCCCGCC
GTGAACGCGCAGTAAGCCGC

Downstream 100 bases:

>100_bases
CATGCGACCGCTGAGGCCTGAAACCTGTCTCCGCCTGCTGAGCGTCCTGGGGCTGATCCCACTTGCCGGCCTGGCGTACG
TCCTGGTCGTGAGTGCCGGG

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLLAVLIFLFTLVLVIWQPKLRWQPGGLGIGWSASLGAVLALLTGVVHLADIPVVWNIVWNATITFVALIIISLILDEAG
FFKWSALHVARWGRGHGHLLFALVILLGAAVSALFANDGTALILTPIVLAMLTALGFRPATTLAFILATGFIADSASLPL
VISNLVNIVSADYFNLDFGQYARVMVPVDLAAILASLGVLYVMFRRDLPARYDPGTLGTPAQAIRDPNVFRVGWIVLVVL
LVGYFAAGPLGVPVSLVAALGAGLLWLVAARGHVVSTRNVLRGAPWQIVIFSLGMYLVVYGLRNAGLTDLLAGVLDRLAQ
GGLWSATLGTGFLTAFLASVMNNMPSVLIGALAIDASQATGAVKQGMVYANVVGNDLGPKITPIGSLATLLWLHVLASKG
IRIGWGQYFRVGIVLTLPVLLVTLAALALRLGG

Sequences:

>Translated_433_residues
MLLAVLIFLFTLVLVIWQPKLRWQPGGLGIGWSASLGAVLALLTGVVHLADIPVVWNIVWNATITFVALIIISLILDEAG
FFKWSALHVARWGRGHGHLLFALVILLGAAVSALFANDGTALILTPIVLAMLTALGFRPATTLAFILATGFIADSASLPL
VISNLVNIVSADYFNLDFGQYARVMVPVDLAAILASLGVLYVMFRRDLPARYDPGTLGTPAQAIRDPNVFRVGWIVLVVL
LVGYFAAGPLGVPVSLVAALGAGLLWLVAARGHVVSTRNVLRGAPWQIVIFSLGMYLVVYGLRNAGLTDLLAGVLDRLAQ
GGLWSATLGTGFLTAFLASVMNNMPSVLIGALAIDASQATGAVKQGMVYANVVGNDLGPKITPIGSLATLLWLHVLASKG
IRIGWGQYFRVGIVLTLPVLLVTLAALALRLGG
>Mature_433_residues
MLLAVLIFLFTLVLVIWQPKLRWQPGGLGIGWSASLGAVLALLTGVVHLADIPVVWNIVWNATITFVALIIISLILDEAG
FFKWSALHVARWGRGHGHLLFALVILLGAAVSALFANDGTALILTPIVLAMLTALGFRPATTLAFILATGFIADSASLPL
VISNLVNIVSADYFNLDFGQYARVMVPVDLAAILASLGVLYVMFRRDLPARYDPGTLGTPAQAIRDPNVFRVGWIVLVVL
LVGYFAAGPLGVPVSLVAALGAGLLWLVAARGHVVSTRNVLRGAPWQIVIFSLGMYLVVYGLRNAGLTDLLAGVLDRLAQ
GGLWSATLGTGFLTAFLASVMNNMPSVLIGALAIDASQATGAVKQGMVYANVVGNDLGPKITPIGSLATLLWLHVLASKG
IRIGWGQYFRVGIVLTLPVLLVTLAALALRLGG

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=431, Percent_Identity=70.5336426914153, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45702; Mature: 45702

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAVLIFLFTLVLVIWQPKLRWQPGGLGIGWSASLGAVLALLTGVVHLADIPVVWNIVW
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NATITFVALIIISLILDEAGFFKWSALHVARWGRGHGHLLFALVILLGAAVSALFANDGT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
ALILTPIVLAMLTALGFRPATTLAFILATGFIADSASLPLVISNLVNIVSADYFNLDFGQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHH
YARVMVPVDLAAILASLGVLYVMFRRDLPARYDPGTLGTPAQAIRDPNVFRVGWIVLVVL
HHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
LVGYFAAGPLGVPVSLVAALGAGLLWLVAARGHVVSTRNVLRGAPWQIVIFSLGMYLVVY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCHHHHHHHHHHHHHHH
GLRNAGLTDLLAGVLDRLAQGGLWSATLGTGFLTAFLASVMNNMPSVLIGALAIDASQAT
HHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHH
GAVKQGMVYANVVGNDLGPKITPIGSLATLLWLHVLASKGIRIGWGQYFRVGIVLTLPVL
HHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHH
LVTLAALALRLGG
HHHHHHHHHHCCC
>Mature Secondary Structure
MLLAVLIFLFTLVLVIWQPKLRWQPGGLGIGWSASLGAVLALLTGVVHLADIPVVWNIVW
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NATITFVALIIISLILDEAGFFKWSALHVARWGRGHGHLLFALVILLGAAVSALFANDGT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
ALILTPIVLAMLTALGFRPATTLAFILATGFIADSASLPLVISNLVNIVSADYFNLDFGQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHH
YARVMVPVDLAAILASLGVLYVMFRRDLPARYDPGTLGTPAQAIRDPNVFRVGWIVLVVL
HHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
LVGYFAAGPLGVPVSLVAALGAGLLWLVAARGHVVSTRNVLRGAPWQIVIFSLGMYLVVY
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCHHHHHHHHHHHHHHH
GLRNAGLTDLLAGVLDRLAQGGLWSATLGTGFLTAFLASVMNNMPSVLIGALAIDASQAT
HHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHH
GAVKQGMVYANVVGNDLGPKITPIGSLATLLWLHVLASKGIRIGWGQYFRVGIVLTLPVL
HHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHH
LVTLAALALRLGG
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]