Click here to switch to the map view.

The map label for this gene is lutB [H]

Identifier: 94314816

GI number: 94314816

Start: 97248

End: 98693

Strand: Reverse

Name: lutB [H]

Synonym: Rmet_5897

Alternate gene names: 94314816

Gene position: 98693-97248 (Counterclockwise)

Preceding gene: 94314817

Following gene: 94314815

Centisome position: 3.83

GC content: 66.87

Gene sequence:

>1446_bases
ATGAGCCAGCAACCCCTGCATTTCGTTCCCACCGAGGACTTTCGCGCCCGCGCGCGCGCCGCGCTCGACGATCCCAAGCT
GCGCAAGAGCTTTCGCGGCGCTATGGACTTCCTCCAGCAGAAGCGGGCCGTGCAGTTCCCCGACGGTGACGAACTCGAGC
ACCTGCGCGACCTCGGCGAAGCCATCCGCCAGCACGCGCTGGCCAATCTGCCCGACCTGCTGGTCGAACTCGAAGGCAAT
CTGACCGCAGCCGGCGTGCAGGTCCACTGGGCCGAGACCGCCGATGAAGCCAACGCGATCATCCTGCGGATCGCCCAGGG
CCACGCCGCCCGGCGCGTGATCAAGGGCAAGTCGATGGCCAGCGAGGAGATGGAACTCAACCATTACCTGGCCGAGCGCG
GCATCGACTGCATCGAATCGGACATGGGCGAGTACATCGTCCAGCTGGCCGGCGAGAAGCCATCGCACATCGTGATGCCG
GCGATCCACAAGACCAAGGCCGATATCGCGACGCTGTTCGAGCAACACATCCCCGATACGCCGTACACGGAAGACGTGGA
TGCGCTGATCCAGACCGGCCGCCGCGCGCTGCGCCAGGCGTTCGTCGAGGCCGATATCGGCCTGTCCGGCGTCAACTTCG
CGGCGGCGGACACCGGCACGCTGTGGCTGGTGGAGAACGAGGGCAACGGCCGTCTTTCCACCACGGTGCCCGACGTGCAT
ATCGCCGTCATGGGGATGGAGAAGGTGGTGGCGAAGCTGTCGCACATCGTGCCGCTCTCCAGCCTGCTGACGCGTTCCGC
GACCGGGCAGCCCATCACCACCTACTTCAACCTGATCTCGAGCCCACGCAAGCCGCATGAGCAGGACGGTCCGCGCGAAG
TCCATCTGGTGCTGCTCGACAACGGCCGCAGCCAGGCATACGCCGACGAGCAGCTACGCGCCACGCTGCAGTGCATTCGC
TGCGGCGCGTGCATGAACCACTGCCCGGTCTACACGCGCATCGGCGGTCATGCCTACGGCACGACTTATCCGGGACCGAT
CGGCAAGATCATCTCGCCCCACCTGCTCGGCCTCGACGCCACGGCCGATCTGGCAACGGCATCGAGCCTGTGTGGCGCGT
GCGGCGAGGTCTGTCCCGTGCGCATTCCGATTCCGCAGCTGCTGGTGCGATTGCGCACTGAGGCGAACCGCAACCCCGAC
GAGGCCGTGCCGCACCCGCTCAAGGGCCAAGGCGCGAAGTTCAGTCGCCGCGAGCACCTGGTGTGGCGCTTCTGGAGCGG
CGCGTTCGCCCATCCCACCACGTACCGCCTGTTCCGCTGGGCCGCCACGCGGCTGCGAGCGCTGACGCCATCGCGGCAAA
TGGGCTGGACGCAGCATCGCAAGCCACTGACGCCGGCGCCAAAGAGCCTTTCCGACCTGCTCGACGCACGCAACCAGCCC
GAGTAG

Upstream 100 bases:

>100_bases
GCCCGTCCAAGACCTCTGACATCCAGCAAACGCTGGCCTACGGCGCGCACGGCCCACGCAGGCTGTGGGTCGTGATCGTG
ACCGACGGAGAAGCCGCGCG

Downstream 100 bases:

>100_bases
CGGCATCGCGCGGCATCCCACCAAAAGAACTCAGACAAACCACCAAGGAGACTTCCAGATGCAACCCTGGACCCAGATCT
ATACGCCGCTGGGCAGCCTC

Product: Iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSQQPLHFVPTEDFRARARAALDDPKLRKSFRGAMDFLQQKRAVQFPDGDELEHLRDLGEAIRQHALANLPDLLVELEGN
LTAAGVQVHWAETADEANAIILRIAQGHAARRVIKGKSMASEEMELNHYLAERGIDCIESDMGEYIVQLAGEKPSHIVMP
AIHKTKADIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVEADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
IAVMGMEKVVAKLSHIVPLSSLLTRSATGQPITTYFNLISSPRKPHEQDGPREVHLVLLDNGRSQAYADEQLRATLQCIR
CGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLDATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPD
EAVPHPLKGQGAKFSRREHLVWRFWSGAFAHPTTYRLFRWAATRLRALTPSRQMGWTQHRKPLTPAPKSLSDLLDARNQP
E

Sequences:

>Translated_481_residues
MSQQPLHFVPTEDFRARARAALDDPKLRKSFRGAMDFLQQKRAVQFPDGDELEHLRDLGEAIRQHALANLPDLLVELEGN
LTAAGVQVHWAETADEANAIILRIAQGHAARRVIKGKSMASEEMELNHYLAERGIDCIESDMGEYIVQLAGEKPSHIVMP
AIHKTKADIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVEADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
IAVMGMEKVVAKLSHIVPLSSLLTRSATGQPITTYFNLISSPRKPHEQDGPREVHLVLLDNGRSQAYADEQLRATLQCIR
CGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLDATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPD
EAVPHPLKGQGAKFSRREHLVWRFWSGAFAHPTTYRLFRWAATRLRALTPSRQMGWTQHRKPLTPAPKSLSDLLDARNQP
E
>Mature_480_residues
SQQPLHFVPTEDFRARARAALDDPKLRKSFRGAMDFLQQKRAVQFPDGDELEHLRDLGEAIRQHALANLPDLLVELEGNL
TAAGVQVHWAETADEANAIILRIAQGHAARRVIKGKSMASEEMELNHYLAERGIDCIESDMGEYIVQLAGEKPSHIVMPA
IHKTKADIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVEADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVHI
AVMGMEKVVAKLSHIVPLSSLLTRSATGQPITTYFNLISSPRKPHEQDGPREVHLVLLDNGRSQAYADEQLRATLQCIRC
GACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLDATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDE
AVPHPLKGQGAKFSRREHLVWRFWSGAFAHPTTYRLFRWAATRLRALTPSRQMGWTQHRKPLTPAPKSLSDLLDARNQPE

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=473, Percent_Identity=39.7463002114165, Blast_Score=341, Evalue=7e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 53110; Mature: 52979

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQPLHFVPTEDFRARARAALDDPKLRKSFRGAMDFLQQKRAVQFPDGDELEHLRDLGE
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AIRQHALANLPDLLVELEGNLTAAGVQVHWAETADEANAIILRIAQGHAARRVIKGKSMA
HHHHHHHHCCCHHHHEECCCEEEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCHHH
SEEMELNHYLAERGIDCIESDMGEYIVQLAGEKPSHIVMPAIHKTKADIATLFEQHIPDT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCC
PYTEDVDALIQTGRRALRQAFVEADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCEEEECCCCEE
IAVMGMEKVVAKLSHIVPLSSLLTRSATGQPITTYFNLISSPRKPHEQDGPREVHLVLLD
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
NGRSQAYADEQLRATLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLDA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHCCCHHCCCCC
TADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDEAVPHPLKGQGAKFSRREHL
HHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCHHHCE
VWRFWSGAFAHPTTYRLFRWAATRLRALTPSRQMGWTQHRKPLTPAPKSLSDLLDARNQP
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCHHCCCCCCCCCHHHHHHHHCCCCC
E
C
>Mature Secondary Structure 
SQQPLHFVPTEDFRARARAALDDPKLRKSFRGAMDFLQQKRAVQFPDGDELEHLRDLGE
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AIRQHALANLPDLLVELEGNLTAAGVQVHWAETADEANAIILRIAQGHAARRVIKGKSMA
HHHHHHHHCCCHHHHEECCCEEEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCHHH
SEEMELNHYLAERGIDCIESDMGEYIVQLAGEKPSHIVMPAIHKTKADIATLFEQHIPDT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCC
PYTEDVDALIQTGRRALRQAFVEADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCEEEECCCCEE
IAVMGMEKVVAKLSHIVPLSSLLTRSATGQPITTYFNLISSPRKPHEQDGPREVHLVLLD
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
NGRSQAYADEQLRATLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLDA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHCCCHHCCCCC
TADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDEAVPHPLKGQGAKFSRREHL
HHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCHHHCE
VWRFWSGAFAHPTTYRLFRWAATRLRALTPSRQMGWTQHRKPLTPAPKSLSDLLDARNQP
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCHHCCCCCCCCCHHHHHHHHCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]