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The map label for this gene is carX

Identifier: 94314563

GI number: 94314563

Start: 2404916

End: 2406352

Strand: Reverse

Name: carX

Synonym: Rmet_5644

Alternate gene names: NA

Gene position: 2406352-2404916 (Counterclockwise)

Preceding gene: 94314566

Following gene: 94314562

Centisome position: 93.27

GC content: 67.01

Gene sequence:

>1437_bases
ATGGAAGACAGCTTGTCCGAAGTGGAAGGTGCAGCGGGTGACGTGAGCGACGTGAGTGAGGTGGATAACTTCGCGCCGGT
CACGCAGGAAGTCGATGCAAGCGATCTCTGCGTGCGTGGCGTGTTGCCAGCGGGGTTGCAGGGCACGTTGTATCGCATCG
GCCCGAACCCGCGCTTTCCGCAGCCCGAGGCGCACTGGTTCGGTGGCGACGGCATGGTGCATGTGTTCGCCATCGACGAT
GGGCGCGTGTCCTACCGCAACCGCTGGGTGCGCACGCCCAAGTGGCGTGACGAGGATCGTGCCGGGCGTCCACTATTCCG
CGCCTTCGCCGGCAAGTTGCCCGATGCGCCGGAGTGGTCGGGCAGCGATGGCGGCGTGGCCAATACCAACATCATCTGGC
ACGGCGGCGCGCTGCTGGCGCTCGAGGAAGCGCACCTGCCCACTGCTGTGACGCCGCGCGCGATCGACACCCTTGGATAT
ATGGAATACGGCCCGCTGAAGGGGCCGTTCACCGCGCATCCGAAGACCGACCCGCGCACTGGCGAACTACTGTTCTTCGG
CTACAACGCCGACGGCAAGCTCACGCCGGGCGTGCAGGTTGGTGCGATCGATGCACAGGGCCGCCTGACGCATTACGCGC
GTCTGCAGGCGCCGTACCCGAGCATGATCCACGACTTCATGGCCACGCCGGGTCATCTGTTGATCCCCGTGCTGCCGCTG
ACCGGTAGCCTTGCGCGCGTGGAGCGCGGCGGCCCTGGCTATGCATGGGAGCCAGAGAAGCCCGCCATGATCGGCGTCCT
GCCGCGTGGTGGCGGCGCTGACGACGTGCGGTGGTTCGAGGGCCCTGCCTGCTATGTGTTCCACGTGATGAACGCGTGGG
AGGAAGGCGAGGGGGGACAACGCATTCTGGCGGACGTCATGGTCTACGACGAACCTCCCCTGTTCCCCCACGCCGACGGA
TCGCCAGGAGATCCCGCCCGGCAGCAGGCGTACCTGACGCGCTGGACGTTCGATCTTGCGTCGGACATACGCCGGTTCAC
CGCCGAACGGCTCGACGATACGGCGGGCGAGTTTCCGCGCATCGACGACCGCGTTTCGGGTTTCCGCTATCGGCACGGAT
GGTTCGCCGCGTCGCATCCCGAGTACGGCGAAGGCGCGCTCAACGGCATCATGCACCTGGACATGCAGGACGGCACGCGC
GACGCCTACTGGCTGCCGCGGGGCGATATCACGTCCGAGCCGGTGTTCGCGCCGGGTGGTGGCGCTCATGGCGGCGAGGG
CGATGGCTGGCTGTTGGCGGTGGTGTGGCGGGCTGCGGCACAATGTAGCGAATTGCTGGTGTTCGATGCGCGGCACGTCG
CGCGCGGTCCGCTGGCAACCGTCGAGCTGCCGCAGCGCGTGCCGTTCGGCTTCCATGGCAACTGGGTAGCGGGATAG

Upstream 100 bases:

>100_bases
GGATGTGCGGTCAGCGGCCTTCGCCGAATCCTTCCTTGACCTCTTAGCACTTGCCACCGATACTTCCCAAACTATTCACT
GTTTAGTTACGGGGGCGTGC

Downstream 100 bases:

>100_bases
CGGGATAGCGAGAGGGATGAACGAAGGCGGCACGAAACAGAACGGCGGGCGCAAGGGTGGTACGCGCAAGGTGGCGGGTG
GCACGACCAAGCCCGCCGGA

Product: Carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEDSLSEVEGAAGDVSDVSEVDNFAPVTQEVDASDLCVRGVLPAGLQGTLYRIGPNPRFPQPEAHWFGGDGMVHVFAIDD
GRVSYRNRWVRTPKWRDEDRAGRPLFRAFAGKLPDAPEWSGSDGGVANTNIIWHGGALLALEEAHLPTAVTPRAIDTLGY
MEYGPLKGPFTAHPKTDPRTGELLFFGYNADGKLTPGVQVGAIDAQGRLTHYARLQAPYPSMIHDFMATPGHLLIPVLPL
TGSLARVERGGPGYAWEPEKPAMIGVLPRGGGADDVRWFEGPACYVFHVMNAWEEGEGGQRILADVMVYDEPPLFPHADG
SPGDPARQQAYLTRWTFDLASDIRRFTAERLDDTAGEFPRIDDRVSGFRYRHGWFAASHPEYGEGALNGIMHLDMQDGTR
DAYWLPRGDITSEPVFAPGGGAHGGEGDGWLLAVVWRAAAQCSELLVFDARHVARGPLATVELPQRVPFGFHGNWVAG

Sequences:

>Translated_478_residues
MEDSLSEVEGAAGDVSDVSEVDNFAPVTQEVDASDLCVRGVLPAGLQGTLYRIGPNPRFPQPEAHWFGGDGMVHVFAIDD
GRVSYRNRWVRTPKWRDEDRAGRPLFRAFAGKLPDAPEWSGSDGGVANTNIIWHGGALLALEEAHLPTAVTPRAIDTLGY
MEYGPLKGPFTAHPKTDPRTGELLFFGYNADGKLTPGVQVGAIDAQGRLTHYARLQAPYPSMIHDFMATPGHLLIPVLPL
TGSLARVERGGPGYAWEPEKPAMIGVLPRGGGADDVRWFEGPACYVFHVMNAWEEGEGGQRILADVMVYDEPPLFPHADG
SPGDPARQQAYLTRWTFDLASDIRRFTAERLDDTAGEFPRIDDRVSGFRYRHGWFAASHPEYGEGALNGIMHLDMQDGTR
DAYWLPRGDITSEPVFAPGGGAHGGEGDGWLLAVVWRAAAQCSELLVFDARHVARGPLATVELPQRVPFGFHGNWVAG
>Mature_478_residues
MEDSLSEVEGAAGDVSDVSEVDNFAPVTQEVDASDLCVRGVLPAGLQGTLYRIGPNPRFPQPEAHWFGGDGMVHVFAIDD
GRVSYRNRWVRTPKWRDEDRAGRPLFRAFAGKLPDAPEWSGSDGGVANTNIIWHGGALLALEEAHLPTAVTPRAIDTLGY
MEYGPLKGPFTAHPKTDPRTGELLFFGYNADGKLTPGVQVGAIDAQGRLTHYARLQAPYPSMIHDFMATPGHLLIPVLPL
TGSLARVERGGPGYAWEPEKPAMIGVLPRGGGADDVRWFEGPACYVFHVMNAWEEGEGGQRILADVMVYDEPPLFPHADG
SPGDPARQQAYLTRWTFDLASDIRRFTAERLDDTAGEFPRIDDRVSGFRYRHGWFAASHPEYGEGALNGIMHLDMQDGTR
DAYWLPRGDITSEPVFAPGGGAHGGEGDGWLLAVVWRAAAQCSELLVFDARHVARGPLATVELPQRVPFGFHGNWVAG

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 52127; Mature: 52127

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDSLSEVEGAAGDVSDVSEVDNFAPVTQEVDASDLCVRGVLPAGLQGTLYRIGPNPRFP
CCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCC
QPEAHWFGGDGMVHVFAIDDGRVSYRNRWVRTPKWRDEDRAGRPLFRAFAGKLPDAPEWS
CCCCEEECCCCEEEEEEECCCCCHHHHCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
GSDGGVANTNIIWHGGALLALEEAHLPTAVTPRAIDTLGYMEYGPLKGPFTAHPKTDPRT
CCCCCEECCEEEEECCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GELLFFGYNADGKLTPGVQVGAIDAQGRLTHYARLQAPYPSMIHDFMATPGHLLIPVLPL
CCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEECC
TGSLARVERGGPGYAWEPEKPAMIGVLPRGGGADDVRWFEGPACYVFHVMNAWEEGEGGQ
CCCHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCEEEEEEEHHHHHCCCCCC
RILADVMVYDEPPLFPHADGSPGDPARQQAYLTRWTFDLASDIRRFTAERLDDTAGEFPR
EEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCC
IDDRVSGFRYRHGWFAASHPEYGEGALNGIMHLDMQDGTRDAYWLPRGDITSEPVFAPGG
CCHHHCCEEEECCEEECCCCCCCCHHHCCEEEEECCCCCCCEEECCCCCCCCCCEECCCC
GAHGGEGDGWLLAVVWRAAAQCSELLVFDARHVARGPLATVELPQRVPFGFHGNWVAG
CCCCCCCCCEEHHHHHHHHHHHHHHEEECCHHHCCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEDSLSEVEGAAGDVSDVSEVDNFAPVTQEVDASDLCVRGVLPAGLQGTLYRIGPNPRFP
CCCCHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCC
QPEAHWFGGDGMVHVFAIDDGRVSYRNRWVRTPKWRDEDRAGRPLFRAFAGKLPDAPEWS
CCCCEEECCCCEEEEEEECCCCCHHHHCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
GSDGGVANTNIIWHGGALLALEEAHLPTAVTPRAIDTLGYMEYGPLKGPFTAHPKTDPRT
CCCCCEECCEEEEECCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GELLFFGYNADGKLTPGVQVGAIDAQGRLTHYARLQAPYPSMIHDFMATPGHLLIPVLPL
CCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEECC
TGSLARVERGGPGYAWEPEKPAMIGVLPRGGGADDVRWFEGPACYVFHVMNAWEEGEGGQ
CCCHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCEEEEEEEHHHHHCCCCCC
RILADVMVYDEPPLFPHADGSPGDPARQQAYLTRWTFDLASDIRRFTAERLDDTAGEFPR
EEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCC
IDDRVSGFRYRHGWFAASHPEYGEGALNGIMHLDMQDGTRDAYWLPRGDITSEPVFAPGG
CCHHHCCEEEECCEEECCCCCCCCHHHCCEEEEECCCCCCCEEECCCCCCCCCCEECCCC
GAHGGEGDGWLLAVVWRAAAQCSELLVFDARHVARGPLATVELPQRVPFGFHGNWVAG
CCCCCCCCCEEHHHHHHHHHHHHHHEEECCHHHCCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]