BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

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The map label for this gene is crt [H]

Identifier: 94314476

GI number: 94314476

Start: 2305091

End: 2305885

Strand: Direct

Name: crt [H]

Synonym: Rmet_5557

Alternate gene names: 94314476

Gene position: 2305091-2305885 (Clockwise)

Preceding gene: 94314475

Following gene: 94314478

Centisome position: 89.34

GC content: 57.23

Gene sequence:

>795_bases
ATGAGCCTACGATATGGCAGCGTCCTCTACGCGGTAAAAGGTAGCGTGGCAATCGTGACGTTAAATCGGCCCGAGTTTCG
CAACGCCTTGGGCGGCACGATTCGGGAAGACATCATCGAAGTGATGGCGGTCGCTGAGGCAAACGATAGCGTTCGAGCCA
TTATTCTCACGGGTGCTGGAAGTGCCTTCTGCTCGGGCGGAGACCTGAACGAGCTGTATCTCCGCGCAGTTCAGGGACAG
ACGATCGCTGAGAAAACAGAGCCCATTCGTGACCGGACACTCCTCGCGGTGTATGAGGCAAAGAAGCCAGTGATAGCGGC
AGTGAATGGCCCGGCCATGGGCGCGGGAATGAACCTGGCACTGGCCGCAGACATTCGTATCGCGTCGAAAGAAGCTCGGT
TCTCCCAAGCTCACACGATGCGCGGGATGATGCCCGACTATGGAGGCACGTATCTGCTACCGGCGCTGTTGGGTAGCTCA
AAGGCGTACGAGCTCATCTGTACAGGCGCCACTTTGGATGCCGAGGAAGCGTTGAGGCTTGGCTTGGTTAGCGATGTGGT
GGAACCCTCGACTCTAATGGATCGAGCCCGCACGATGGCTCAAGCTATTGCGCTCAACGCTCCTATTCCGATTCGCTTGG
CGAAACGAGCCGTCCAACAGCACAATCTCGGAGGGTTGCGCGAAGCCTTGGCCCGCGAGACTGCTGCCCAGAACGTCTGC
TATGAATCCCAAGATGCTCGCGAAGGATTACGATCCTTCCTCGAGAAGCGAACTCCGACATTCCAAGGGAAATAA

Upstream 100 bases:

>100_bases
ATAGGCTGCTGACGACGGCAGAAGACATCTCGCGACAGTGGGAATCCACAAACAACAGGTAACCCCTGCCCTCTTTCAGA
GCGCAGAACATGGAGAAAAG

Downstream 100 bases:

>100_bases
TTTCCACTCCGAAGCGGCGCGGCGCTAGAAAATGCCGGAGCTATACCGCGGCCGACTAGGTCTTGCCGGTCTCCGGATCG
CGGTACTGCGGAGATGACGA

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSLRYGSVLYAVKGSVAIVTLNRPEFRNALGGTIREDIIEVMAVAEANDSVRAIILTGAGSAFCSGGDLNELYLRAVQGQ
TIAEKTEPIRDRTLLAVYEAKKPVIAAVNGPAMGAGMNLALAADIRIASKEARFSQAHTMRGMMPDYGGTYLLPALLGSS
KAYELICTGATLDAEEALRLGLVSDVVEPSTLMDRARTMAQAIALNAPIPIRLAKRAVQQHNLGGLREALARETAAQNVC
YESQDAREGLRSFLEKRTPTFQGK

Sequences:

>Translated_264_residues
MSLRYGSVLYAVKGSVAIVTLNRPEFRNALGGTIREDIIEVMAVAEANDSVRAIILTGAGSAFCSGGDLNELYLRAVQGQ
TIAEKTEPIRDRTLLAVYEAKKPVIAAVNGPAMGAGMNLALAADIRIASKEARFSQAHTMRGMMPDYGGTYLLPALLGSS
KAYELICTGATLDAEEALRLGLVSDVVEPSTLMDRARTMAQAIALNAPIPIRLAKRAVQQHNLGGLREALARETAAQNVC
YESQDAREGLRSFLEKRTPTFQGK
>Mature_263_residues
SLRYGSVLYAVKGSVAIVTLNRPEFRNALGGTIREDIIEVMAVAEANDSVRAIILTGAGSAFCSGGDLNELYLRAVQGQT
IAEKTEPIRDRTLLAVYEAKKPVIAAVNGPAMGAGMNLALAADIRIASKEARFSQAHTMRGMMPDYGGTYLLPALLGSSK
AYELICTGATLDAEEALRLGLVSDVVEPSTLMDRARTMAQAIALNAPIPIRLAKRAVQQHNLGGLREALARETAAQNVCY
ESQDAREGLRSFLEKRTPTFQGK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=33.8709677419355, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI37594469, Length=206, Percent_Identity=32.5242718446602, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI37594471, Length=207, Percent_Identity=32.8502415458937, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI221136756, Length=237, Percent_Identity=28.2700421940928, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI221136753, Length=237, Percent_Identity=28.2700421940928, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=31.7204301075269, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI221307494, Length=237, Percent_Identity=28.2700421940928, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=25.8823529411765, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=25.8823529411765, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=25.8823529411765, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=32.156862745098, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=27.34375, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=27.34375, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI70995211, Length=207, Percent_Identity=31.8840579710145, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4757966, Length=250, Percent_Identity=28, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI51538581, Length=250, Percent_Identity=28, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI68989263, Length=208, Percent_Identity=31.25, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI4757968, Length=256, Percent_Identity=26.5625, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI49355787, Length=256, Percent_Identity=26.5625, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=31.5384615384615, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116235487, Length=205, Percent_Identity=32.6829268292683, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=28.125, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=28.125, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=204, Percent_Identity=27.9411764705882, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=33.7349397590361, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=36.6412213740458, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=35.408560311284, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=31.1023622047244, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082183, Length=271, Percent_Identity=29.1512915129151, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790281, Length=205, Percent_Identity=30.2439024390244, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=29.2682926829268, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=33.59375, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=34.8178137651822, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=33.4801762114537, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=233, Percent_Identity=32.618025751073, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=248, Percent_Identity=28.2258064516129, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=215, Percent_Identity=29.7674418604651, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=29.3193717277487, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=29.3193717277487, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=28.9617486338798, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=29.8578199052133, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=184, Percent_Identity=28.2608695652174, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=184, Percent_Identity=28.2608695652174, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=184, Percent_Identity=28.2608695652174, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=25, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=27.319587628866, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=33.8645418326693, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=33.8645418326693, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=32.6923076923077, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=33.6363636363636, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24654903, Length=188, Percent_Identity=27.1276595744681, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357171, Length=205, Percent_Identity=30.2439024390244, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24650670, Length=203, Percent_Identity=32.0197044334975, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=27.1317829457364, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=189, Percent_Identity=30.6878306878307, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571730, Length=189, Percent_Identity=30.6878306878307, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28283; Mature: 28152

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRYGSVLYAVKGSVAIVTLNRPEFRNALGGTIREDIIEVMAVAEANDSVRAIILTGAG
CCCCCCCEEEEECCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
SAFCSGGDLNELYLRAVQGQTIAEKTEPIRDRTLLAVYEAKKPVIAAVNGPAMGAGMNLA
CCEECCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCEE
LAADIRIASKEARFSQAHTMRGMMPDYGGTYLLPALLGSSKAYELICTGATLDAEEALRL
EEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
GLVSDVVEPSTLMDRARTMAQAIALNAPIPIRLAKRAVQQHNLGGLREALARETAAQNVC
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
YESQDAREGLRSFLEKRTPTFQGK
CCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLRYGSVLYAVKGSVAIVTLNRPEFRNALGGTIREDIIEVMAVAEANDSVRAIILTGAG
CCCCCCEEEEECCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
SAFCSGGDLNELYLRAVQGQTIAEKTEPIRDRTLLAVYEAKKPVIAAVNGPAMGAGMNLA
CCEECCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCEE
LAADIRIASKEARFSQAHTMRGMMPDYGGTYLLPALLGSSKAYELICTGATLDAEEALRL
EEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
GLVSDVVEPSTLMDRARTMAQAIALNAPIPIRLAKRAVQQHNLGGLREALARETAAQNVC
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
YESQDAREGLRSFLEKRTPTFQGK
CCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]