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The map label for this gene is vanB [H]

Identifier: 94314436

GI number: 94314436

Start: 2263614

End: 2264609

Strand: Reverse

Name: vanB [H]

Synonym: Rmet_5517

Alternate gene names: 94314436

Gene position: 2264609-2263614 (Counterclockwise)

Preceding gene: 94314437

Following gene: 94314435

Centisome position: 87.77

GC content: 57.03

Gene sequence:

>996_bases
ATGGAGACAGAGATGAGCAACCATGCAGGTGACGCGCCGTTCCTTGAGCTAAGACTCTCCGAGAGACGAAAGGAGGCCGA
CGGCATCTTGTCATTCGAGTTGGTGCATCCTGACGGGGAGCAACTGCCAGCTTTCGACGCCGGCGCACATATTCTGGTGG
ACATTGTTCCCGGAGTATCTCGAGCGTACTCCCTGTGCAACGATCCAGCCGAGCGACATCGCTATGTTATTGCCGTGCTC
CGCACTGAAGATTCACGAGGCGGGTCCGTCGGCATGCATCAGGCCATCGAACCTGGCCAACTGGTCCGAGTAAGTGCGCC
ACGAAACGAGTTCGAGTTGGTCCAGAGCGCGGGCCGTAGCCTGCTTTTCGCCGGCGGAATTGGGATTACCCCGCTGCTGT
CAATGGCTGAGACGCTAGCAAATGCAGATCGAGAGTTTGAACTCCACTACTGCACACGCGACGCGGCGAAGACGGCCTTC
ACGTCACGCATAGAGACGCGGTTCTCTAAGCAAGCCCGCATCTATCACGACCTGGCGTCGAAAGAACAGCCATTCGATGC
GCGAAAAGTATTGAAGCAGGGCGGCTCCGATGATCATGTGTACGCCTGCGGACCGTCGGGCTTCATCGAACATATCTTGT
CGACTGCGGCAGAGCTCGGTTGGGAAAAGCGCCAGCTACATAGAGAGTTCTTCGGTTCACCAACTACACAGAATGATGGC
AGTGCTGAGACTGCTTTCGATTTGATTCTGGCCTCGTCTGGGAAAAAGGTGCACGTGCCTTGCGGCGTGAGCGCCGCTAC
TGCGCTCCTTGAGGCGGGGATCTCGCTCTCGATGTCCTGCGAGCAAGGAATTTGTGGAACCTGCGTTACCACAGTTCTGA
ACGGTATGCCTGATCACCGGGACCATTACCTGACCGACGATGACAGAAGCCGAAACGATTGCTTCATGCCGTGCTGCTCG
CGGTCGCTGACGGCGGAGCTACTGGTTGACCTTTGA

Upstream 100 bases:

>100_bases
GCAAAGTTGACCGATTCGGTCATCGTCGTTTCCTGCACGGAACTCTAAGCTATCTCCACGCATTTTCCGGCCCATCTGAC
GAGGGACGGGAGCGAGAACG

Downstream 100 bases:

>100_bases
AGATATCAAGAAAAACTGAGGAGACGAGAATGACGATTGCTTCTGATTTCGATACTGAGCTGGCAAGCCACGAAATCTAT
TCCGATCCAGAGCGGATGCA

Product: Vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
METEMSNHAGDAPFLELRLSERRKEADGILSFELVHPDGEQLPAFDAGAHILVDIVPGVSRAYSLCNDPAERHRYVIAVL
RTEDSRGGSVGMHQAIEPGQLVRVSAPRNEFELVQSAGRSLLFAGGIGITPLLSMAETLANADREFELHYCTRDAAKTAF
TSRIETRFSKQARIYHDLASKEQPFDARKVLKQGGSDDHVYACGPSGFIEHILSTAAELGWEKRQLHREFFGSPTTQNDG
SAETAFDLILASSGKKVHVPCGVSAATALLEAGISLSMSCEQGICGTCVTTVLNGMPDHRDHYLTDDDRSRNDCFMPCCS
RSLTAELLVDL

Sequences:

>Translated_331_residues
METEMSNHAGDAPFLELRLSERRKEADGILSFELVHPDGEQLPAFDAGAHILVDIVPGVSRAYSLCNDPAERHRYVIAVL
RTEDSRGGSVGMHQAIEPGQLVRVSAPRNEFELVQSAGRSLLFAGGIGITPLLSMAETLANADREFELHYCTRDAAKTAF
TSRIETRFSKQARIYHDLASKEQPFDARKVLKQGGSDDHVYACGPSGFIEHILSTAAELGWEKRQLHREFFGSPTTQNDG
SAETAFDLILASSGKKVHVPCGVSAATALLEAGISLSMSCEQGICGTCVTTVLNGMPDHRDHYLTDDDRSRNDCFMPCCS
RSLTAELLVDL
>Mature_331_residues
METEMSNHAGDAPFLELRLSERRKEADGILSFELVHPDGEQLPAFDAGAHILVDIVPGVSRAYSLCNDPAERHRYVIAVL
RTEDSRGGSVGMHQAIEPGQLVRVSAPRNEFELVQSAGRSLLFAGGIGITPLLSMAETLANADREFELHYCTRDAAKTAF
TSRIETRFSKQARIYHDLASKEQPFDARKVLKQGGSDDHVYACGPSGFIEHILSTAAELGWEKRQLHREFFGSPTTQNDG
SAETAFDLILASSGKKVHVPCGVSAATALLEAGISLSMSCEQGICGTCVTTVLNGMPDHRDHYLTDDDRSRNDCFMPCCS
RSLTAELLVDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=325, Percent_Identity=35.0769230769231, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787658, Length=302, Percent_Identity=24.5033112582781, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 36112; Mature: 36112

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METEMSNHAGDAPFLELRLSERRKEADGILSFELVHPDGEQLPAFDAGAHILVDIVPGVS
CCCCCCCCCCCCCEEEHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHH
RAYSLCNDPAERHRYVIAVLRTEDSRGGSVGMHQAIEPGQLVRVSAPRNEFELVQSAGRS
HHHHHHCCHHHCCEEEEEEEEECCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHCCCE
LLFAGGIGITPLLSMAETLANADREFELHYCTRDAAKTAFTSRIETRFSKQARIYHDLAS
EEEECCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KEQPFDARKVLKQGGSDDHVYACGPSGFIEHILSTAAELGWEKRQLHREFFGSPTTQNDG
CCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
SAETAFDLILASSGKKVHVPCGVSAATALLEAGISLSMSCEQGICGTCVTTVLNGMPDHR
CHHHHHHHHEECCCCEEEECCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCC
DHYLTDDDRSRNDCFMPCCSRSLTAELLVDL
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHCC
>Mature Secondary Structure
METEMSNHAGDAPFLELRLSERRKEADGILSFELVHPDGEQLPAFDAGAHILVDIVPGVS
CCCCCCCCCCCCCEEEHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHH
RAYSLCNDPAERHRYVIAVLRTEDSRGGSVGMHQAIEPGQLVRVSAPRNEFELVQSAGRS
HHHHHHCCHHHCCEEEEEEEEECCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHCCCE
LLFAGGIGITPLLSMAETLANADREFELHYCTRDAAKTAFTSRIETRFSKQARIYHDLAS
EEEECCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KEQPFDARKVLKQGGSDDHVYACGPSGFIEHILSTAAELGWEKRQLHREFFGSPTTQNDG
CCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
SAETAFDLILASSGKKVHVPCGVSAATALLEAGISLSMSCEQGICGTCVTTVLNGMPDHR
CHHHHHHHHEECCCCEEEECCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCC
DHYLTDDDRSRNDCFMPCCSRSLTAELLVDL
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]