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The map label for this gene is tnpB [C]

Identifier: 94314416

GI number: 94314416

Start: 2241553

End: 2242404

Strand: Direct

Name: tnpB [C]

Synonym: Rmet_5497

Alternate gene names: 94314416

Gene position: 2241553-2242404 (Clockwise)

Preceding gene: 94314415

Following gene: 94314418

Centisome position: 86.88

GC content: 58.22

Gene sequence:

>852_bases
ATGCGCTCCCACATCAGTGAATATTGCCTGCGCACGATGTGTCGGGTACTGGGCGTTCATCGTAGCGGTTACTACGTCTG
GCAACGCCAGGCATCCAGCCCACGCCGGCGGGAGGATGATCGGCTGCTGGGTCTGATCAAGCATCACTGGTTGGCCAGCG
GTGGCGTCTATGGCTACCGCAAGATCACGGTAGATCTGCGTGAATCTGGCGAAGCTTGCAGCCGTCACCGTGTGCATCGA
CTGATGAAAGGAGAAGGCCTGCGCGCACAGGTTGGTTACGGCAGCAAGCCACGCTATCGTGGCGGTCCGGTCGGCGTGGT
GGCGAACGTCTTGAATCGAGAGTTCGCCCCCGATGCGCCGAACAAGGTCTGGGTCACCGACATCACGTACATCCGAACCT
ACGAAGGCTGGCTGTATCTGGCGGCAGTCATGGATTTGTACTCGCGTCAGATCGTTGGCTGGGCCACGCGATCAACGATG
ACCAGCGATCTGGTATTGCAGGCCTTGCTAGCCGCAGTCTGGAAACGCAAACCGAGGGCAGGTGTTATGGTGCATTCGGA
TCAGGGCAGCCAGTTCACCAGCGATGATTGGCAGTCATTCCTGAAGGCACACAACATGGTGCCGAGCATGAGTCGTCGCG
GTAATTGTCACGATAACGCCGTTGCCGAGAGCTTCTTCAGTGCATTGAAGAAGGAGCGAATCAAGCGGCGCATCTACCCG
AACAGGGCGACTGCTGCCACGGACGTATTCGATTACATTGAGATGTTCTATAACCCGATTCGCCGGCACGGTTCCGCCGG
CGACCTGTCACCTGTAGAGTTTGAGCGGCGCTACGCGCTAAACGGCTCGTGA

Upstream 100 bases:

>100_bases
GACGACTGAAGGCCGAGCTCAAGCGCGTGACAGAGGAGCGCGACATTCTAAAAAAAGCCGCCGCGTACTTTGCAAAGGGA
TAGCGGCAAGGTACGCATTC

Downstream 100 bases:

>100_bases
GTGTCTATGAAACTCTGGCCGGTCCAACGATCACGCCCGCTACCTCTGAGCCTGGGATGAAAGGCACCGCCGGCTTGACC
TGATAGCGGCCGCGGACAAT

Product: transposase catalytic site ISRme11

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRSHISEYCLRTMCRVLGVHRSGYYVWQRQASSPRRREDDRLLGLIKHHWLASGGVYGYRKITVDLRESGEACSRHRVHR
LMKGEGLRAQVGYGSKPRYRGGPVGVVANVLNREFAPDAPNKVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTM
TSDLVLQALLAAVWKRKPRAGVMVHSDQGSQFTSDDWQSFLKAHNMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYP
NRATAATDVFDYIEMFYNPIRRHGSAGDLSPVEFERRYALNGS

Sequences:

>Translated_283_residues
MRSHISEYCLRTMCRVLGVHRSGYYVWQRQASSPRRREDDRLLGLIKHHWLASGGVYGYRKITVDLRESGEACSRHRVHR
LMKGEGLRAQVGYGSKPRYRGGPVGVVANVLNREFAPDAPNKVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTM
TSDLVLQALLAAVWKRKPRAGVMVHSDQGSQFTSDDWQSFLKAHNMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYP
NRATAATDVFDYIEMFYNPIRRHGSAGDLSPVEFERRYALNGS
>Mature_283_residues
MRSHISEYCLRTMCRVLGVHRSGYYVWQRQASSPRRREDDRLLGLIKHHWLASGGVYGYRKITVDLRESGEACSRHRVHR
LMKGEGLRAQVGYGSKPRYRGGPVGVVANVLNREFAPDAPNKVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTM
TSDLVLQALLAAVWKRKPRAGVMVHSDQGSQFTSDDWQSFLKAHNMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYP
NRATAATDVFDYIEMFYNPIRRHGSAGDLSPVEFERRYALNGS

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=234, Percent_Identity=35.042735042735, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788405, Length=280, Percent_Identity=40.3571428571429, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1787263, Length=280, Percent_Identity=40.3571428571429, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1786753, Length=280, Percent_Identity=40.3571428571429, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1786570, Length=280, Percent_Identity=40.3571428571429, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1786490, Length=280, Percent_Identity=40.3571428571429, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=32.9588014981273, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790724, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789424, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789224, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788305, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787669, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786558, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32452; Mature: 32452

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHISEYCLRTMCRVLGVHRSGYYVWQRQASSPRRREDDRLLGLIKHHWLASGGVYGYR
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
KITVDLRESGEACSRHRVHRLMKGEGLRAQVGYGSKPRYRGGPVGVVANVLNREFAPDAP
EEEEEHHHCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
NKVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTMTSDLVLQALLAAVWKRKPRA
CEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVMVHSDQGSQFTSDDWQSFLKAHNMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYP
CEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NRATAATDVFDYIEMFYNPIRRHGSAGDLSPVEFERRYALNGS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MRSHISEYCLRTMCRVLGVHRSGYYVWQRQASSPRRREDDRLLGLIKHHWLASGGVYGYR
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
KITVDLRESGEACSRHRVHRLMKGEGLRAQVGYGSKPRYRGGPVGVVANVLNREFAPDAP
EEEEEHHHCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
NKVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTMTSDLVLQALLAAVWKRKPRA
CEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVMVHSDQGSQFTSDDWQSFLKAHNMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYP
CEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NRATAATDVFDYIEMFYNPIRRHGSAGDLSPVEFERRYALNGS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]