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The map label for this gene is lcfA [H]

Identifier: 94314300

GI number: 94314300

Start: 2106926

End: 2108458

Strand: Reverse

Name: lcfA [H]

Synonym: Rmet_5381

Alternate gene names: 94314300

Gene position: 2108458-2106926 (Counterclockwise)

Preceding gene: 94314301

Following gene: 94314299

Centisome position: 81.72

GC content: 67.32

Gene sequence:

>1533_bases
ATGCTTCCGATTGAACTGTTCTGGCGGGCCGCCGACCGCTGGCCCGACAACGTCGCCATTGACGCCCCCGGCGGCAGCCT
GCGCTATCGCGAACTGGCCGGCCAGGTGGCGGCGCTGGCCACGCATTTCATGACGCTCGATCCCGCGCCGCAGAGTCGCG
TGGGCATCTGCGCCGGCAACTCGGCCGAGCACATCGTGGCGCTGCTGGCGGTGCTGGCCAGCGGCAAGATCTGGGTGCCG
CTGAACCCGAAGAGCACTCAGCCCGAGTTGCGCCGCATCATCGACGCCACGACGCCGAGCATCGTGGTACTCGATGCCGC
CTCCGAAGCGCTGCTGGGCGATGCACCGGGCACGCGGCTCTACACGGACTATGCGGACCATGCGTCAGGCGGCGCAGACG
TGCTGAGCGCCGTTGTTCTCCGGTATCCCGATGCCACGCGCCCGGTGCTCGATCTGCACCGGGACGCCACGCAGGCCATC
AAGTTCACAGGCGGTACCACGGGCGCGCCCAAGGGCGTCATGCAGCCGTATCGCGCCTGGATGACCAATATCGTCAACCA
GATCCACGCCTGGGGCTTCACCGAGGCGGATCGCTATGTGATGGCCGCGCCGATCACGCATGGCACGTCCACCTACGTCA
TGCCGATCCTGGCGCAGGGCGGTTGTCACGTGATCATGGAAGGCAGCGGGGCAGAAGCGGTGCGCGCCACGTTCCGTGAC
CGCGCCGGCACGGTCTGCTTTATGCCGCCCACGCTGATCTACATGCTGATGGCGCTGCCCGGCACATCGCGCGCCGACTA
CCCGGCGCTGCGTCGCCTGATTTACGGCGGCGCGCCGATGCCGCCGCAGAAGATCCAGCAGGCGCGCGAGTTCTTCGGGT
CGGTGGTGGCCACCACATATGGCCAGACCGAAGCGCCGCAACTGCTGACGGTGATCGGCCCCGACGATCTGGCTGACGAG
CGCCGCATGGCATCGGTGGGGCGCGCCACGTGGTTCAGCGAGGTGGCGATCATGTCCCCCGACGGGCGTCTGCTGCCCCG
TGGCGAGGTGGGCGAAGTGGTGGCGCGTGGCGATCTGGTCATGACCGGCTACTGGAACCGCCCGGACCTGACCGCCGCGA
CCATCGTCGACGGCTGGTTGCATACCGGGGATCGTGGCATGTTGGACGCGCAGGGTTTCCTGTATCTGAAGGACCGCCTG
CGCGAAGTGGTGATCACCGGCGGCTTCAACGTCTACCCGATCGACGTGGAGAACGCGCTGGGCCAGCACCCGGCCGTGCA
TGAATGCGCGGTGTTCGGCGTGCCCGACGACAAATGGGGCGAAGCGGTCCATGCGGCCGTGCAATTGCGCGAAGGATGCG
CGGTGCAGGCCACCGAGCTGATCGCGTTCGTGCGCGAGCGGCTGGGTCCGGTGCAGACGCCAAAGCAGATTCATTTCCAC
ACCAGCCTGCCGCGCTCCACCGTCGGCAAGGTACTCAAGAACACCGTGCGCGACCAGGCGCTGACGGCATTGCAAACCGG
AGACCAAGCATGA

Upstream 100 bases:

>100_bases
GAGCAACTCCGTACCTTCGCGGCGTCCGAGATCAAGCGTTGGCACGACGTGGTCAAGCAATCGGGCGCGCGTGTCAACTG
AGGATTCCCAAGGACCATCC

Downstream 100 bases:

>100_bases
GCCAACAAGACAAGCCGGTGACCCGGCTGTGCACCCACACGCTGACCAGCCTGCACTATCGCGAGGCCGATCTCGTGGAC
GACCTTCTGGGCAAGAAGAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLPIELFWRAADRWPDNVAIDAPGGSLRYRELAGQVAALATHFMTLDPAPQSRVGICAGNSAEHIVALLAVLASGKIWVP
LNPKSTQPELRRIIDATTPSIVVLDAASEALLGDAPGTRLYTDYADHASGGADVLSAVVLRYPDATRPVLDLHRDATQAI
KFTGGTTGAPKGVMQPYRAWMTNIVNQIHAWGFTEADRYVMAAPITHGTSTYVMPILAQGGCHVIMEGSGAEAVRATFRD
RAGTVCFMPPTLIYMLMALPGTSRADYPALRRLIYGGAPMPPQKIQQAREFFGSVVATTYGQTEAPQLLTVIGPDDLADE
RRMASVGRATWFSEVAIMSPDGRLLPRGEVGEVVARGDLVMTGYWNRPDLTAATIVDGWLHTGDRGMLDAQGFLYLKDRL
REVVITGGFNVYPIDVENALGQHPAVHECAVFGVPDDKWGEAVHAAVQLREGCAVQATELIAFVRERLGPVQTPKQIHFH
TSLPRSTVGKVLKNTVRDQALTALQTGDQA

Sequences:

>Translated_510_residues
MLPIELFWRAADRWPDNVAIDAPGGSLRYRELAGQVAALATHFMTLDPAPQSRVGICAGNSAEHIVALLAVLASGKIWVP
LNPKSTQPELRRIIDATTPSIVVLDAASEALLGDAPGTRLYTDYADHASGGADVLSAVVLRYPDATRPVLDLHRDATQAI
KFTGGTTGAPKGVMQPYRAWMTNIVNQIHAWGFTEADRYVMAAPITHGTSTYVMPILAQGGCHVIMEGSGAEAVRATFRD
RAGTVCFMPPTLIYMLMALPGTSRADYPALRRLIYGGAPMPPQKIQQAREFFGSVVATTYGQTEAPQLLTVIGPDDLADE
RRMASVGRATWFSEVAIMSPDGRLLPRGEVGEVVARGDLVMTGYWNRPDLTAATIVDGWLHTGDRGMLDAQGFLYLKDRL
REVVITGGFNVYPIDVENALGQHPAVHECAVFGVPDDKWGEAVHAAVQLREGCAVQATELIAFVRERLGPVQTPKQIHFH
TSLPRSTVGKVLKNTVRDQALTALQTGDQA
>Mature_510_residues
MLPIELFWRAADRWPDNVAIDAPGGSLRYRELAGQVAALATHFMTLDPAPQSRVGICAGNSAEHIVALLAVLASGKIWVP
LNPKSTQPELRRIIDATTPSIVVLDAASEALLGDAPGTRLYTDYADHASGGADVLSAVVLRYPDATRPVLDLHRDATQAI
KFTGGTTGAPKGVMQPYRAWMTNIVNQIHAWGFTEADRYVMAAPITHGTSTYVMPILAQGGCHVIMEGSGAEAVRATFRD
RAGTVCFMPPTLIYMLMALPGTSRADYPALRRLIYGGAPMPPQKIQQAREFFGSVVATTYGQTEAPQLLTVIGPDDLADE
RRMASVGRATWFSEVAIMSPDGRLLPRGEVGEVVARGDLVMTGYWNRPDLTAATIVDGWLHTGDRGMLDAQGFLYLKDRL
REVVITGGFNVYPIDVENALGQHPAVHECAVFGVPDDKWGEAVHAAVQLREGCAVQATELIAFVRERLGPVQTPKQIHFH
TSLPRSTVGKVLKNTVRDQALTALQTGDQA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=520, Percent_Identity=27.6923076923077, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI187761345, Length=530, Percent_Identity=26.6037735849057, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI187761343, Length=530, Percent_Identity=26.6037735849057, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI122937307, Length=501, Percent_Identity=25.5489021956088, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI38505220, Length=469, Percent_Identity=24.3070362473348, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI42544132, Length=456, Percent_Identity=25.8771929824561, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI58082049, Length=477, Percent_Identity=25.1572327044025, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI157311624, Length=477, Percent_Identity=25.1572327044025, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157311622, Length=477, Percent_Identity=25.1572327044025, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI115511026, Length=439, Percent_Identity=25.5125284738041, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI13375727, Length=579, Percent_Identity=22.9706390328152, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI27477105, Length=325, Percent_Identity=28.9230769230769, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8923896, Length=372, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI253970489, Length=372, Percent_Identity=25.8064516129032, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=541, Percent_Identity=28.2809611829945, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI221142682, Length=484, Percent_Identity=28.099173553719, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=25.968992248062, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=25.296442687747, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790505, Length=526, Percent_Identity=24.3346007604563, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788595, Length=476, Percent_Identity=24.5798319327731, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786801, Length=392, Percent_Identity=23.9795918367347, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789201, Length=349, Percent_Identity=25.214899713467, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786529, Length=555, Percent_Identity=25.045045045045, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=438, Percent_Identity=28.5388127853881, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI32563687, Length=434, Percent_Identity=28.8018433179723, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17559526, Length=372, Percent_Identity=27.6881720430108, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=540, Percent_Identity=26.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=540, Percent_Identity=26.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=540, Percent_Identity=26.6666666666667, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=520, Percent_Identity=27.5, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=26.3358778625954, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=514, Percent_Identity=24.7081712062257, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=530, Percent_Identity=21.3207547169811, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=500, Percent_Identity=23, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=350, Percent_Identity=22.8571428571429, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564090, Length=473, Percent_Identity=23.4672304439746, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=382, Percent_Identity=24.869109947644, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17510401, Length=168, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=529, Percent_Identity=25.7088846880907, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323182, Length=554, Percent_Identity=24.5487364620939, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=560, Percent_Identity=25.3571428571429, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=26.5107212475634, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859661, Length=523, Percent_Identity=25.8126195028681, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24653035, Length=512, Percent_Identity=23.4375, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=25.1533742331288, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161076582, Length=521, Percent_Identity=23.4165067178503, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=23.1225296442688, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24648253, Length=484, Percent_Identity=25.2066115702479, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648255, Length=484, Percent_Identity=25, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922652, Length=498, Percent_Identity=25.7028112449799, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648260, Length=465, Percent_Identity=25.3763440860215, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356441, Length=483, Percent_Identity=24.223602484472, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24656500, Length=509, Percent_Identity=24.5579567779961, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648257, Length=466, Percent_Identity=26.3948497854077, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130357, Length=514, Percent_Identity=22.9571984435798, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281365686, Length=227, Percent_Identity=25.9911894273128, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21358303, Length=460, Percent_Identity=22.6086956521739, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24667959, Length=380, Percent_Identity=23.9473684210526, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472339, Length=380, Percent_Identity=23.9473684210526, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24667955, Length=380, Percent_Identity=23.9473684210526, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921316, Length=169, Percent_Identity=32.5443786982249, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55106; Mature: 55106

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPIELFWRAADRWPDNVAIDAPGGSLRYRELAGQVAALATHFMTLDPAPQSRVGICAGN
CCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECC
SAEHIVALLAVLASGKIWVPLNPKSTQPELRRIIDATTPSIVVLDAASEALLGDAPGTRL
CHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCCE
YTDYADHASGGADVLSAVVLRYPDATRPVLDLHRDATQAIKFTGGTTGAPKGVMQPYRAW
EECCHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHCHHHHEEECCCCCCCCHHHHHHHHHH
MTNIVNQIHAWGFTEADRYVMAAPITHGTSTYVMPILAQGGCHVIMEGSGAEAVRATFRD
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHH
RAGTVCFMPPTLIYMLMALPGTSRADYPALRRLIYGGAPMPPQKIQQAREFFGSVVATTY
CCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GQTEAPQLLTVIGPDDLADERRMASVGRATWFSEVAIMSPDGRLLPRGEVGEVVARGDLV
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCHHHHEECCCEE
MTGYWNRPDLTAATIVDGWLHTGDRGMLDAQGFLYLKDRLREVVITGGFNVYPIDVENAL
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEEHHHHC
GQHPAVHECAVFGVPDDKWGEAVHAAVQLREGCAVQATELIAFVRERLGPVQTPKQIHFH
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
TSLPRSTVGKVLKNTVRDQALTALQTGDQA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLPIELFWRAADRWPDNVAIDAPGGSLRYRELAGQVAALATHFMTLDPAPQSRVGICAGN
CCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECC
SAEHIVALLAVLASGKIWVPLNPKSTQPELRRIIDATTPSIVVLDAASEALLGDAPGTRL
CHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCCE
YTDYADHASGGADVLSAVVLRYPDATRPVLDLHRDATQAIKFTGGTTGAPKGVMQPYRAW
EECCHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHCHHHHEEECCCCCCCCHHHHHHHHHH
MTNIVNQIHAWGFTEADRYVMAAPITHGTSTYVMPILAQGGCHVIMEGSGAEAVRATFRD
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHH
RAGTVCFMPPTLIYMLMALPGTSRADYPALRRLIYGGAPMPPQKIQQAREFFGSVVATTY
CCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GQTEAPQLLTVIGPDDLADERRMASVGRATWFSEVAIMSPDGRLLPRGEVGEVVARGDLV
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCHHHHEECCCEE
MTGYWNRPDLTAATIVDGWLHTGDRGMLDAQGFLYLKDRLREVVITGGFNVYPIDVENAL
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEEHHHHC
GQHPAVHECAVFGVPDDKWGEAVHAAVQLREGCAVQATELIAFVRERLGPVQTPKQIHFH
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
TSLPRSTVGKVLKNTVRDQALTALQTGDQA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]