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The map label for this gene is benM [H]

Identifier: 94314288

GI number: 94314288

Start: 2092658

End: 2093740

Strand: Direct

Name: benM [H]

Synonym: Rmet_5369

Alternate gene names: 94314288

Gene position: 2092658-2093740 (Clockwise)

Preceding gene: 94314285

Following gene: 94314289

Centisome position: 81.11

GC content: 65.1

Gene sequence:

>1083_bases
ATGTGTCTCCTGAGCAGGAATGCCTCCCATGGTACGAACGGCAGCCGCCCCAGCTTTGACGAATCGAGAAAGCTCCCGCG
CCAGAACGTGTATGCGACGCGCAAGCGGCTGGTGCATACTCCGGCTGGCACAGCCATCGCGCCAGCTTTTCCAAATCGAG
CACGCACACCCATGGACATCGCCTCGCTTGCCATCCTGATCGCCGCTATCGAGGAGAAGAGTCTTTCACGCGCGGCCGAG
CGCGAGAATCTGGTCACCTCCGCGGCCAGCAAGCGGATTGCCGAACTGGAACGTCGCGCGGGCACGGCGCTGCTGCGCCG
CCATGGACGCGGCGTGGAGCCGACACCGGCAGGCGCCATGCTGTATCAGCGTGCCAAGGCCATCGTGCGCAGCGTGCAAC
TGGCGCAGGACGCGCTGGCGTCCTACTCGATGGACGGCATCGCCAAAATTCGCCTGGCATCGAACCGGTCGACGATCCTG
CAGTTCCTGCCGACCGATATCAGCGGCTTTCTCAAGCACGACCCCGACGCCCGCATCGACCTGATCGAGGCGTTCAGCTT
CGACATTCCGCGCATGGTGGCCAACGGCGACGCCGACATCGGCATCTATCACGCGCGCAGCCCGAGCCCGGGCGTGTACT
CCCTGCCCTACCGCAAGGACCGAGTCGGACTGGTCGTGCCGCGCGGCCACCCGCTGGAGGCGAAAGGGTCGCTACGGCTC
GAGGAAGCACTGGACTACGACTTCCTTGGCTACTTCCCACGCCATACGTTCGACGCCTTCCTCACCCTGGTGTCGGGCAC
GATCTCTCGCCCGCTGACCGTGAAGACACAGGTCTCGAACTTCGAGGCCCGCTGCCGCATGGTGCGTGAAGGCCTGGGCC
TTGCCGTGGTGCCGGAGGGCATCGCCAGGAACTACCTGCAGATGATGGGGCTGTCGCTGCTGACACTGCGCGATGCTTGG
GCCGAGCGCGAGTTCTACGTCTGCGTGCGCGACAAGGAAAGCCTGCCGTCCGGCGTGGCCCGGCTGCTCGAATACCTGAG
CCGCTGCGAGGCGATGGAGCAGCATTTCCTGAAGCCGCGATGA

Upstream 100 bases:

>100_bases
AGTCGATTACAGCGCGTCGCGCGTGGTGCATGACGTCGTCCGCCAACGTCGCGCGGGCCTGGTCGCCGACGTACTCGGCA
AAATCGTCGATCAGCATTCC

Downstream 100 bases:

>100_bases
AGTCTGCGTTTCCATATCGTCAAAGCTCGGGTACGTCAGCTCTCTAAGATGAAAAACAGCCACCCTGCCTTGAAAGGGGG
GCGCTGATTACAAGATTCAT

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MCLLSRNASHGTNGSRPSFDESRKLPRQNVYATRKRLVHTPAGTAIAPAFPNRARTPMDIASLAILIAAIEEKSLSRAAE
RENLVTSAASKRIAELERRAGTALLRRHGRGVEPTPAGAMLYQRAKAIVRSVQLAQDALASYSMDGIAKIRLASNRSTIL
QFLPTDISGFLKHDPDARIDLIEAFSFDIPRMVANGDADIGIYHARSPSPGVYSLPYRKDRVGLVVPRGHPLEAKGSLRL
EEALDYDFLGYFPRHTFDAFLTLVSGTISRPLTVKTQVSNFEARCRMVREGLGLAVVPEGIARNYLQMMGLSLLTLRDAW
AEREFYVCVRDKESLPSGVARLLEYLSRCEAMEQHFLKPR

Sequences:

>Translated_360_residues
MCLLSRNASHGTNGSRPSFDESRKLPRQNVYATRKRLVHTPAGTAIAPAFPNRARTPMDIASLAILIAAIEEKSLSRAAE
RENLVTSAASKRIAELERRAGTALLRRHGRGVEPTPAGAMLYQRAKAIVRSVQLAQDALASYSMDGIAKIRLASNRSTIL
QFLPTDISGFLKHDPDARIDLIEAFSFDIPRMVANGDADIGIYHARSPSPGVYSLPYRKDRVGLVVPRGHPLEAKGSLRL
EEALDYDFLGYFPRHTFDAFLTLVSGTISRPLTVKTQVSNFEARCRMVREGLGLAVVPEGIARNYLQMMGLSLLTLRDAW
AEREFYVCVRDKESLPSGVARLLEYLSRCEAMEQHFLKPR
>Mature_360_residues
MCLLSRNASHGTNGSRPSFDESRKLPRQNVYATRKRLVHTPAGTAIAPAFPNRARTPMDIASLAILIAAIEEKSLSRAAE
RENLVTSAASKRIAELERRAGTALLRRHGRGVEPTPAGAMLYQRAKAIVRSVQLAQDALASYSMDGIAKIRLASNRSTIL
QFLPTDISGFLKHDPDARIDLIEAFSFDIPRMVANGDADIGIYHARSPSPGVYSLPYRKDRVGLVVPRGHPLEAKGSLRL
EEALDYDFLGYFPRHTFDAFLTLVSGTISRPLTVKTQVSNFEARCRMVREGLGLAVVPEGIARNYLQMMGLSLLTLRDAW
AEREFYVCVRDKESLPSGVARLLEYLSRCEAMEQHFLKPR

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=278, Percent_Identity=24.8201438848921, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=26.3779527559055, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=275, Percent_Identity=26.5454545454545, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=296, Percent_Identity=21.2837837837838, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=197, Percent_Identity=27.4111675126904, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 39948; Mature: 39948

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLLSRNASHGTNGSRPSFDESRKLPRQNVYATRKRLVHTPAGTAIAPAFPNRARTPMDI
CEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHEECCCCCEECCCCCCCCCCCHHH
ASLAILIAAIEEKSLSRAAERENLVTSAASKRIAELERRAGTALLRRHGRGVEPTPAGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LYQRAKAIVRSVQLAQDALASYSMDGIAKIRLASNRSTILQFLPTDISGFLKHDPDARID
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCHHHHHCCCCCCHHH
LIEAFSFDIPRMVANGDADIGIYHARSPSPGVYSLPYRKDRVGLVVPRGHPLEAKGSLRL
HHHHHCCCCCHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCEEH
EEALDYDFLGYFPRHTFDAFLTLVSGTISRPLTVKTQVSNFEARCRMVREGLGLAVVPEG
HHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCEEEECHH
IARNYLQMMGLSLLTLRDAWAEREFYVCVRDKESLPSGVARLLEYLSRCEAMEQHFLKPR
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCLLSRNASHGTNGSRPSFDESRKLPRQNVYATRKRLVHTPAGTAIAPAFPNRARTPMDI
CEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHEECCCCCEECCCCCCCCCCCHHH
ASLAILIAAIEEKSLSRAAERENLVTSAASKRIAELERRAGTALLRRHGRGVEPTPAGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
LYQRAKAIVRSVQLAQDALASYSMDGIAKIRLASNRSTILQFLPTDISGFLKHDPDARID
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCHHHHHCCCCCCHHH
LIEAFSFDIPRMVANGDADIGIYHARSPSPGVYSLPYRKDRVGLVVPRGHPLEAKGSLRL
HHHHHCCCCCHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCEEECCCCCCCCCCCEEH
EEALDYDFLGYFPRHTFDAFLTLVSGTISRPLTVKTQVSNFEARCRMVREGLGLAVVPEG
HHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCEEEECHH
IARNYLQMMGLSLLTLRDAWAEREFYVCVRDKESLPSGVARLLEYLSRCEAMEQHFLKPR
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]