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The map label for this gene is fliN [H]

Identifier: 94314218

GI number: 94314218

Start: 2002183

End: 2002653

Strand: Direct

Name: fliN [H]

Synonym: Rmet_5299

Alternate gene names: 94314218

Gene position: 2002183-2002653 (Clockwise)

Preceding gene: 94314217

Following gene: 94314219

Centisome position: 77.6

GC content: 64.97

Gene sequence:

>471_bases
ATGACTGACGGCACCCAGGACAAGCCGGTCGATCCAATGGACGACTGGGCCAGCGCCCTCGCGGAGCAGACCAGCGCCGA
GCAGGCAACGTCCACTGCCGCGCCGGCGCCCAGCGCTGCCGCACCGGCGGCGCAAGCCATGCCTGCCGCCACGCCGGCAG
CTTCCACGGTATTCCCGCCGCTGGCGCAGGAAGCGCCGCCGGGGTTCCACAACGATATCGGGATGATTCTCGATATTCCC
GTGCAGCTCACGGTGGAACTCGGCCGCACCAAGGTACCCATCAAGAACCTGCTGCAACTGGCGCAAGGTTCGGTCGTGGA
ACTCGACGGCCTGGCCGGCGAACCGATGGACGTGCTTGTCAACGGCTACCTGATCGCCCAGGGCGAAGTGGTGGTGGTCA
ATGACAAGTTCGGCATCCGCCTGACGGACATCATCACGCCGTCGGAACGTATCCGGAAGCTCAACAAATGA

Upstream 100 bases:

>100_bases
GTCATGCAGTGCGGCTTCGGCACCATGAACGGCCAGTACGCGCTGCGCGTGGAACGGATGCTCAATTACCAAGACAGCGA
TTCCAACAAGGAAACCGATC

Downstream 100 bases:

>100_bases
TGGTTGCCGCGCGCCGGCTTGCCGCGGGCATTGCCCTTGCGGGCGTTGCCGCTTCCGGCGCGCAGGCTGCCGACGTCGTA
CTCAGCACGGCCCAGGCGGG

Product: Flagellar motor switch FliN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTDGTQDKPVDPMDDWASALAEQTSAEQATSTAAPAPSAAAPAAQAMPAATPAASTVFPPLAQEAPPGFHNDIGMILDIP
VQLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK

Sequences:

>Translated_156_residues
MTDGTQDKPVDPMDDWASALAEQTSAEQATSTAAPAPSAAAPAAQAMPAATPAASTVFPPLAQEAPPGFHNDIGMILDIP
VQLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK
>Mature_155_residues
TDGTQDKPVDPMDDWASALAEQTSAEQATSTAAPAPSAAAPAAQAMPAATPAASTVFPPLAQEAPPGFHNDIGMILDIPV
QLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=145, Percent_Identity=54.4827586206897, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 16294; Mature: 16163

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGTQDKPVDPMDDWASALAEQTSAEQATSTAAPAPSAAAPAAQAMPAATPAASTVFPP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
LAQEAPPGFHNDIGMILDIPVQLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLV
CHHHCCCCCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCHHEEE
NGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK
ECEEEECCCEEEEECCCCEEEEEEECCHHHHHHCCC
>Mature Secondary Structure 
TDGTQDKPVDPMDDWASALAEQTSAEQATSTAAPAPSAAAPAAQAMPAATPAASTVFPP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
LAQEAPPGFHNDIGMILDIPVQLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLV
CHHHCCCCCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCHHEEE
NGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK
ECEEEECCCEEEEECCCCEEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]