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The map label for this gene is fliM [H]

Identifier: 94314217

GI number: 94314217

Start: 2001180

End: 2002190

Strand: Direct

Name: fliM [H]

Synonym: Rmet_5298

Alternate gene names: 94314217

Gene position: 2001180-2002190 (Clockwise)

Preceding gene: 94314216

Following gene: 94314218

Centisome position: 77.56

GC content: 63.4

Gene sequence:

>1011_bases
ATGGCCTACGACAAGTTCCTCTCGCAGGACGAGGTAGATGAACTACTGAAAGGCGTATCGGGCGAATCCGATGCGCCCGT
GGCAAGCGCGCCGGTCGCCGACGATGGCGGCGTGCGTCCGTACAACCTGGCCAACCAGGAGCGCATCGTCCGCGGCCGCC
TGCACACGCTCGAGATCATCAACGAGCGTTTTGCCCGCGCCCTGCGCACCGCGCTGTTCAACTTCATCCGCCGCGGCGCC
GACATCTCGGTAGGCACCGTGCGGATCGAGAAGTACGGCGATTTCGTGCGCAACCTGCCCGTCCCGACCAACCTGAACCT
GGTCCACATGAAGCCGCTGCGGGGCACCGCGCTATTCGTGTACGACCCGAACCTGGTGTTTCTCGTCGTCGACAACCTGT
TCGGCGGCGATGGCCGTTTCCACACCCGCGTGGAAGGCCGCGACTTTACCCAGACCGAGCAGCGCATCATCCGCCGCATG
CTTGACCTCACGCTTTCCAGCTACGGCCAGGCCTGGCGCACCGTGCATCCGATCGAGTTCGACTATGTCCGCTCGGAGAT
GCACACGAAGTTCGCCAACGTCGCCGGCCCGAACGATGCCGTGGTCACCACCGCGTTCCACATCGAACTGGGCGCCGTGG
GCGGGCAGCTTCATATCTGCATCCCGTTCTCGATGATCGAGCCACTGCGCGATCTGCTGATGAACCCGCTGCAGGACGAG
GTCGAGGTGGACAAGCGCTGGCTCGGCCAGCTCTCCAACCAGCTACGTGCCGCCGAAGTGGAACTGGTGGCCGAATTCAC
GCACCTGCGCAGCACCGTGGCCGAGGTCCTGGCAATGCGCGAAGGCGACGTGCTGCCGATCGAACTGCCGGAACACATCT
TCGGCAAGGTGAACGGCGTGCCCGTCATGCAGTGCGGCTTCGGCACCATGAACGGCCAGTACGCGCTGCGCGTGGAACGG
ATGCTCAATTACCAAGACAGCGATTCCAACAAGGAAACCGATCATGACTGA

Upstream 100 bases:

>100_bases
TGCCCGCCGAACTTCACCTCTGCCGCCGCATCGCGCGCGGCGCCTGAAGCCGGCCATGGACCGTTTCATCCTTAAAGCCG
TTCACGGGGCAGGACACTAG

Downstream 100 bases:

>100_bases
CGGCACCCAGGACAAGCCGGTCGATCCAATGGACGACTGGGCCAGCGCCCTCGCGGAGCAGACCAGCGCCGAGCAGGCAA
CGTCCACTGCCGCGCCGGCG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAYDKFLSQDEVDELLKGVSGESDAPVASAPVADDGGVRPYNLANQERIVRGRLHTLEIINERFARALRTALFNFIRRGA
DISVGTVRIEKYGDFVRNLPVPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIRRM
LDLTLSSYGQAWRTVHPIEFDYVRSEMHTKFANVAGPNDAVVTTAFHIELGAVGGQLHICIPFSMIEPLRDLLMNPLQDE
VEVDKRWLGQLSNQLRAAEVELVAEFTHLRSTVAEVLAMREGDVLPIELPEHIFGKVNGVPVMQCGFGTMNGQYALRVER
MLNYQDSDSNKETDHD

Sequences:

>Translated_336_residues
MAYDKFLSQDEVDELLKGVSGESDAPVASAPVADDGGVRPYNLANQERIVRGRLHTLEIINERFARALRTALFNFIRRGA
DISVGTVRIEKYGDFVRNLPVPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIRRM
LDLTLSSYGQAWRTVHPIEFDYVRSEMHTKFANVAGPNDAVVTTAFHIELGAVGGQLHICIPFSMIEPLRDLLMNPLQDE
VEVDKRWLGQLSNQLRAAEVELVAEFTHLRSTVAEVLAMREGDVLPIELPEHIFGKVNGVPVMQCGFGTMNGQYALRVER
MLNYQDSDSNKETDHD
>Mature_335_residues
AYDKFLSQDEVDELLKGVSGESDAPVASAPVADDGGVRPYNLANQERIVRGRLHTLEIINERFARALRTALFNFIRRGAD
ISVGTVRIEKYGDFVRNLPVPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIRRML
DLTLSSYGQAWRTVHPIEFDYVRSEMHTKFANVAGPNDAVVTTAFHIELGAVGGQLHICIPFSMIEPLRDLLMNPLQDEV
EVDKRWLGQLSNQLRAAEVELVAEFTHLRSTVAEVLAMREGDVLPIELPEHIFGKVNGVPVMQCGFGTMNGQYALRVERM
LNYQDSDSNKETDHD

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=331, Percent_Identity=53.7764350453172, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37852; Mature: 37720

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDKFLSQDEVDELLKGVSGESDAPVASAPVADDGGVRPYNLANQERIVRGRLHTLEII
CCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NERFARALRTALFNFIRRGADISVGTVRIEKYGDFVRNLPVPTNLNLVHMKPLRGTALFV
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCEEEEECCCCCCEEEE
YDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIRRMLDLTLSSYGQAWRTVHPIEF
ECCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DYVRSEMHTKFANVAGPNDAVVTTAFHIELGAVGGQLHICIPFSMIEPLRDLLMNPLQDE
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEEEECHHHHHHHHHHHHCCCHHH
VEVDKRWLGQLSNQLRAAEVELVAEFTHLRSTVAEVLAMREGDVLPIELPEHIFGKVNGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCC
PVMQCGFGTMNGQYALRVERMLNYQDSDSNKETDHD
EEEEECCCCCCCCCEEEHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYDKFLSQDEVDELLKGVSGESDAPVASAPVADDGGVRPYNLANQERIVRGRLHTLEII
CCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
NERFARALRTALFNFIRRGADISVGTVRIEKYGDFVRNLPVPTNLNLVHMKPLRGTALFV
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCEEEEECCCCCCEEEE
YDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIRRMLDLTLSSYGQAWRTVHPIEF
ECCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DYVRSEMHTKFANVAGPNDAVVTTAFHIELGAVGGQLHICIPFSMIEPLRDLLMNPLQDE
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEEEECHHHHHHHHHHHHCCCHHH
VEVDKRWLGQLSNQLRAAEVELVAEFTHLRSTVAEVLAMREGDVLPIELPEHIFGKVNGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCC
PVMQCGFGTMNGQYALRVERMLNYQDSDSNKETDHD
EEEEECCCCCCCCCEEEHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]